DOCSLIB.ORG

Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems Sarah Weeda1, Na Zhang2, Xiaolei Zhao3, Grace Ndip4, Yangdong Guo2*, Gregory A. Buck5, Conggui Fu3, Shuxin Ren1* 1 Agriculture Research Station, Virginia State University, Petersburg, Virginia, United States of America, 2 College of Agriculture and Biotechnology, China Agricultural University, Beijing, P. R. China, 3 Tianjin Crop Science Institute, Tianjin Academy of Agricultural Science, Tianjin, P. R. China, 4 Department of Chemistry, Virginia State University, Petersburg, Virginia, United States of America, 5 Center of the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia, United States of America Abstract Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression. To comprehensively investigate the role(s) of melatonin at the genomics level, we utilized mRNA-seq technology to analyze Arabidopsis plants subjected to a 16-hour 100 pM (low) and 1 mM (high) melatonin treatment. The expression profiles were analyzed to identify differentially expressed genes. 100 pM melatonin treatment significantly affected the expression of only 81 genes with 51 down-regulated and 30 up-regulated. However, 1 mM melatonin significantly altered 1308 genes with 566 up-regulated and 742 down-regulated. Not all genes altered by low melatonin were affected by high melatonin, indicating different roles of melatonin in regulation of plant growth and development under low and high concentrations. Furthermore, a large number of genes altered by melatonin were involved in plant stress defense. Transcript levels for many stress receptors, kinases, and stress-associated calcium signals were up-regulated. The majority of transcription factors identified were also involved in plant stress defense. Additionally, most identified genes in ABA, ET, SA and JA pathways were up-regulated, while genes pertaining to auxin responses and signaling, peroxidases, and those associated with cell wall synthesis and modifications were mostly down- regulated. Our results indicate critical roles of melatonin in plant defense against various environmental stresses, and provide a framework for functional analysis of genes in melatonin-mediated signaling pathways. Citation: Weeda S, Zhang N, Zhao X, Ndip G, Guo Y, et al. (2014) Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems. PLoS ONE 9(3): e93462. doi:10.1371/journal.pone.0093462 Editor: Cynthia Gibas, University of North Carolina at Charlotte, United States of America Received January 8, 2014; Accepted March 6, 2014; Published March 28, 2014 Copyright: ß 2014 Weeda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work is partially supported by USDA Evans Allen Fund. No additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: [email protected] (YG); [email protected] (SR) Introduction demonstrated a peak in melatonin levels late in the day [19], indicating its biosynthesis in light. Furthermore, melatonin Melatonin (N-acetyl-5-methoxytryptamine) is a versatile mole- biosynthesis occurred under constant light in senescent rice leaves cule that, since its 1958 discovery in bovine pineal glands [1], has and was nearly undetectable under constant darkness [22]. Other been found across kingdom lines including bacteria, fungi, and reports found no significant correlation with melatonin levels and plants [2–5]. Its numerous and diverse functions include regulating day/night cycles [18]. Interestingly, developing sweet cherries circadian rhythms and seasonal reproduction cycles, promoting exhibited a dual peak of melatonin levels, one nocturnal and one sleep, preventing diabetes, attenuating cancer cell proliferation, in late day [20]. Contradicting reports of melatonin levels in and enhancing immunity [6–10]. Melatonin has also been ripening fruits add to the variation observed among plant species; characterized as a potent antioxidant capable of scavenging melatonin levels decreased in ripening cherries [20], but increased reactive oxygen species (ROS) and reactive nitrogen species (RNS) in ripening tomatoes [18]. The possible role of melatonin in [11–14]. regulating flowering has also been investigated [23–25]; however Since the relatively recent discovery of melatonin in plants, an unequivocal role of melatonin in photoperiod-dependent investigations to elucidate its function in plants has been driven by processes in plants has not yet been established what is known in animals. One such area of focus is the Melatonin has been studied extensively as an antioxidant in involvement of melatonin in modulating circadian rhythms and mammals. Many studies demonstrate the ability of melatonin to photoperiod-dependent processes. While melatonin levels do protect against many human diseases, including those linked to appear to be affected by light/dark cycles in some plants, the oxidative stress [26–27]. Melatonin was able to attenuate pattern varies among species, tissues, and organs [15–21]. The nocturnal peak of melatonin characteristic of humans and animals paraquat-induced lung and liver damage in rats [28–29] and has been observed in Chenopodium rubrum [16], grape skins [17] and Parkinson’s disease in mice [30]. Furthermore, exogenously Ulva sp [21]. Conversely, studies conducted in water hyacinth applied melatonin can enhance the production of antioxidative PLOS ONE | www.plosone.org 1 March 2014 | Volume 9 | Issue 3 | e93462 MT-Mediated Stress Defense in Arabidopsis enzymes such as glutathione peroxidase and superoxide dismutase Arabidopsis will provide us basic information to genetically dissect [31]. Melatonin may similarly play a protective role against melatonin-mediated signaling pathway(s) in regulating plant oxidative stress in plants. Oxidative stress is capable of inducing growth and development. elevated melatonin levels in various plant species [17,32–34]. Indeed, the daytime peak of melatonin levels found in sweet cherry Materials and Methods was associated with high temperature and light intensity, suggesting melatonin was synthesized in response to oxidative Plant materials and growth condition stress [17]. Transgenic rice seedlings with elevated levels of Arabidopsis thaliana ecotype Columbia (Col-0) was used for RNA- melatonin were more resistant to herbicide induced oxidative seq experiments and oxidative stress experiments. All plants were stress than their wild type counterparts [35]. Furthermore, grown in a growth-chamber under 23uC and 14/10 hours light/ oxidative stress induced the expression of genes involved in dark cycle. Three-week-old seedlings were removed from soil, melatonin biosynthesis, leading to increased melatonin production rinsed, and submerged in100 pM, or 1 mM melatonin for in both wild type and transgenic rice [35]. Melatonin also appears 16 hours with gentle shaking. Mock solution was used as control. to protect plants against UV and ozone damage [36–40], All experiments were duplicated for statistical analysis. attenuate photo-oxidation of the photosynthetic system, and, at moderate levels, protect chlorophyll during senescence [39–42]. In RNA extraction, library construction and sequencing addition, melatonin can promote low temperature and osmotic After melatonin treatment, total RNA was extracted using the stress tolerance [43–48], alleviate copper damage [49–50], and QIAGEN RNeasy Mini Kit according to the manufacturer’s improve salt tolerance [51] and fungal disease resistance [52] in a instructions (QIAGEN). Purification of mRNA from total RNA diversity of plant species. was conducted using an Oligotex mRNA Mini Kit (QIAGEN). The structure of melatonin is another feature that has driven The mRNA was then used to construct cDNA libraries using the investigations into its function in plants. Melatonin is structurally mRNA-Seq Sample Preparation KitTM (Illumina) following similar to the plant hormone indole-3-acetic acid (IAA) and has standard protocols. Briefly, the mRNA was fragmented by many features that make it a candidate for a functional auxin [53– exposure to divalent cations at 94uC and the fragmented mRNA 54]. In addition, melatonin and auxin biosynthetic pathways share was converted into double stranded cDNA. The cDNA ends were the same precursor, tryptophan [55]. Since auxins play critical polished, the 39-hydroxls extended with A bases, and ligated to roles as growth regulators during plant development such as shoot Illumina-specific adapter-primers. The adaptor ligated DNA was elongation, lateral root formation, and cell expansion, much work amplified by 15 cycles of PCR followed by purification using has focused on the effect of melatonin on these processes QiagenTM PCR purification kit to obtain the final library for [42,48,56–63]. Investigations have shown that melatonin and its sequencing. The DNA yield and fragment insert size distribution precursor serotonin
Load more

Recommended publications
  • The Janus-Like Role of Proline Metabolism in Cancer Lynsey Burke1,Innaguterman1, Raquel Palacios Gallego1, Robert G
    Burke et al. Cell Death Discovery (2020) 6:104 https://doi.org/10.1038/s41420-020-00341-8 Cell Death Discovery REVIEW ARTICLE Open Access The Janus-like role of proline metabolism in cancer Lynsey Burke1,InnaGuterman1, Raquel Palacios Gallego1, Robert G. Britton1, Daniel Burschowsky2, Cristina Tufarelli1 and Alessandro Rufini1 Abstract The metabolism of the non-essential amino acid L-proline is emerging as a key pathway in the metabolic rewiring that sustains cancer cells proliferation, survival and metastatic spread. Pyrroline-5-carboxylate reductase (PYCR) and proline dehydrogenase (PRODH) enzymes, which catalyze the last step in proline biosynthesis and the first step of its catabolism, respectively, have been extensively associated with the progression of several malignancies, and have been exposed as potential targets for anticancer drug development. As investigations into the links between proline metabolism and cancer accumulate, the complexity, and sometimes contradictory nature of this interaction emerge. It is clear that the role of proline metabolism enzymes in cancer depends on tumor type, with different cancers and cancer-related phenotypes displaying different dependencies on these enzymes. Unexpectedly, the outcome of rewiring proline metabolism also differs between conditions of nutrient and oxygen limitation. Here, we provide a comprehensive review of proline metabolism in cancer; we collate the experimental evidence that links proline metabolism with the different aspects of cancer progression and critically discuss the potential mechanisms involved. ● How is the rewiring of proline metabolism regulated Facts depending on cancer type and cancer subtype? 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; ● Is it possible to develop successful pharmacological ● Proline metabolism is widely rewired during cancer inhibitor of proline metabolism enzymes for development.
    [Show full text]
  • A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
    biomolecules Review S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains Krzysztof Brzezinski Laboratory of Structural Microbiology, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland; [email protected] Received: 16 November 2020; Accepted: 15 December 2020; Published: 16 December 2020 Abstract: S-adenosyl-l-homocysteine hydrolase (SAHase) is a major regulator of cellular methylation reactions that occur in eukaryotic and prokaryotic organisms. SAHase activity is also a significant source of l-homocysteine and adenosine, two compounds involved in numerous vital, as well as pathological processes. Therefore, apart from cellular methylation, the enzyme may also influence other processes important for the physiology of particular organisms. Herein, presented is the structural characterization and comparison of SAHases of eukaryotic and prokaryotic origin, with an emphasis on the two principal domains of SAHase subunit based on the Rossmann motif. The first domain is involved in the binding of a substrate, e.g., S-adenosyl-l-homocysteine or adenosine and the second domain binds the NAD+ cofactor. Despite their structural similarity, the molecular interactions between an adenosine-based ligand molecule and macromolecular environment are different in each domain. As a consequence, significant differences in the conformation of d-ribofuranose rings of nucleoside and nucleotide ligands, especially those attached to adenosine moiety, are observed. On the other hand, the chemical nature of adenine ring recognition, as well as an orientation of the adenine ring around the N-glycosidic bond are of high similarity for the ligands bound in the substrate- and cofactor-binding domains.
    [Show full text]
  • 02 Whole.Pdf (8.685Mb)
    Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the Author. X-RAY CRYSTALLOGRAPHIC INVESTIGATIONS OF THE STRUCTURES OF ENZYMES OF MEDICAL AND BIOTECHNOLOGICA L IMPORTANCE by Richard Lawrence Kingston A dissertation submitted in partial satisfaction of the requirements fo r the degree of Doctor of Philosophy in the Department of Biochemistry at MASSEY UNIVERSITY, NEW ZEALAND November, 1996 ABSTRACT This thesis is broadly in three parts. In the first, the problem of identifying conditions under which a protein will crystallize is considered. Then structural studies on two enzymes are reported, glucose-fructose oxidoreductase from the bacterium Zymomonas mobilis, and the human bile salt dependent lipase (carboxyl ester hydrolase). The ability of protein crystals to diffract X-rays provides the experimental data required to determine their three dimensional structures at atomic resolution. However the crystalliza­ tion of proteins is not always straightforward. A systematic procedure to search for protein crystallization conditions has been developed. This procedure is based on the use of orthog­ onal arrays (matrices whose columns possess certain balancing properties). The theoretical and practical background to the problem is discussed, and the relationship of the presented procedure to other published search methods is considered. The anaerobic Gram-negative bacterium Zymomonas mobilis occurs naturally in sugar-rich growth media, and has attracted much interest because of its potential for industrial ethanol production. In this organism the periplasmic enzyme glucose-fructose oxidoreductase (GFOR) is involved in a protective mechanism to counter osmotic stress.
    [Show full text]
  • PREDICTION REPORTS Successful Recognition of Protein Folds Using Threading Methods Biased by Sequence Similari
    PROTEINS: Structure, Function, and Genetics Suppl 3:104–111 (1999) FOLD RECOGNITION: PREDICTION REPORTS Successful Recognition of Protein Folds Using Threading Methods Biased by Sequence Similarity and Predicted Secondary Structure David T. Jones,* Michael Tress, Kevin Bryson, and Caroline Hadley Department of Biological Sciences, University of Warwick, Coventry, United Kingdom ABSTRACT Analysis of our fold recognition sequence families includes a member of known 3D struc- results in the 3rd Critical Assessment in Structure ture. Sensitive sequence profile methods can extend the Prediction (CASP3) experiment, using the programs range of comparative modeling to the point where around THREADER 2 and GenTHREADER, shows an en- 12% of the families can be assigned to a known 3D couraging level of overall success. Of the 23 submit- structural superfamily, but in contrast to this, it is esti- ted predictions, 20 targets showed no clear se- mated that the probability of a novel protein having a quence similarity to proteins of known 3D structure. similar fold to a known structure is currently as high as These 20 targets can be divided into 22 domains, of 60–70%. There is, therefore, a great deal to be gained from which, 20 domains either entirely match a previ- attempting to solve the problem of building models for very ously known fold, or partially match a substantial remotely related proteins, and for proteins which have region of a known fold. Of these 20 domains, we merely analogous folds, and so are beyond the scope of correctly assigned the folds in 10 cases. Proteins normal comparative modeling strategies. To date the most Suppl 1999:3:104–111.
    [Show full text]
  • That Is the Question
    polymers Review To Tie or Not to Tie? That Is the Question Pawel Dabrowski-Tumanski 1,2 and Joanna I. Sulkowska 1,2,* 1 Centre of New Technologies, University of Warsaw, Warsaw 02-097, Poland; [email protected] 2 Faculty of Chemistry, University of Warsaw, Warsaw 02-093, Poland * Correspondence: [email protected]; Tel.: +48-22-55-43678 Received: 26 August 2017; Accepted: 12 September 2017; Published: 16 September 2017 Abstract: In this review, we provide an overview of entangled proteins. Around 6% of protein structures deposited in the PBD are entangled, forming knots, slipknots, lassos and links. We present theoretical methods and tools that enabled discovering and classifying such structures. We discuss the advantages and disadvantages of the non-trivial topology in proteins, based on available data about folding, stability, biological properties and evolutionary conservation. We also formulate intriguing and challenging questions on the border of biophysics, bioinformatics, biology and mathematics, which arise from the discovery of an entanglement in proteins. Finally, we discuss possible applications of entangled proteins in medicine and nanotechnology, such as the chance to design super stable proteins, whose stability could be controlled by chemical potential. Keywords: knots; link; lasso; topology; polymer; disulfide 1. Introduction Self-tying of proteins was long considered as impossible, as it requires the threading of a chain through a twisted loop, which in principle is an entropically unfavorable event. However, similar structures have been known to exist in other biopolymers (such as DNA) for many years [1,2], and their self-tying was attributed to, e.g., better packing [3].
    [Show full text]
  • Α+Β 31 37 28 18 16 12 40 3
    class Fold# EC SC HI SS HP MJ MP MG total Fam. PDB Rep. Struc. Name α/β 18 60 46 23 40 19 743 202 16 183 1xel - NAD(P)-binding Rossmann Fold α/β 24 20 69 17 19 17 16 10 11 179 13 132 1gky - P-loop Containing NTP Hydrolases α+β 31 37 28 18 16 12 40 33 157 23 160 1fxd - like Ferrodoxin α/β 01 45 36 13 22 11 10 54 146 37 399 1byb - TIM-barrel α/β 23 18 17 794822 67 5 36 1pyd a:2-181 Thiamin-binding α/β 04 15 11 7 10 1955 63 13 132 2tmd a:490-645 FAD/NAD(P)-binding α+β 55 89789366 56 4 23 1sry a:111-421 Class-II-aaRS/Biotin Synthetases β 27 7 10 884433 47 5 19 1fnb 19-154 Reductase/Elongation Factor Domain β 24 13 7433333 39 18 177 1snc - OB-fold α+β 11 10 8482221 37 11 48 1igd - beta-Grasp β 55 9 10 552222 37 7 19 1bdo - Barrel-sandwich hybrid α/β 15 55445633 35 3 22 2ts1 1-217 ATP pyrophoshatases α/β 05 10 4242223 29 4 35 1zym a: The "swivelling" beta/beta/alpha domain α/β 60 57463211 29 3 18 3pmg a:1-190 Phosphoglucomutase, first 3 domains α+β 68 42364243 28 2 3 1mat - Creatinase/methionine aminopeptidase α+β 39 64344111 24 3 42 1gad o:149-312 like G3P dehydrogenase, Ct-dom α+β 18 54412212 21 3 23 1fkd - FKBP-like α/β 41 33331311 18 3 16 1opr - Phosphoribosyltransferases (PRTases) α 78 19121111 17 1 23 1oel a:(*) GroEL, the ATPase domain α+β 10 22242122 17 2 5 1dar 477-599 Ribosomal protein S5 domain 2-like α+β 43 43221122 17 4 50 3grs 364-478 FAD/NAD-linked reductases, dimer-dom.
    [Show full text]
  • Supporting Information
    Supporting Information Rackovsky 10.1073/pnas.0903433106 Table S1. The 10 property factors 1. Alpha-helix/bend preference 2. Side-chain size 3. Extended structure preference 4. Hydrophobicity 5. Double-bend preference 6. Amino acid composition 7. Flat extended preference 8. Occurrence in ␣ region 9. pK 10. Surrounding hydrophobicity The first 4 property factors correspond essentially to single amino acid physical properties. The remaining 6 factors are superpositions of several physical properties and for convenience are identified by the property making the greatest contribution to the factor. Rackovsky www.pnas.org/cgi/content/short/0903433106 1of4 Table S2. The 59 CAT groups C A T Number of sequences CATH group name 1 20 5 39 Single alpha-helices involved in coiled coils or other helix–helix interfaces 1 50 10 31 Glycosyltransferase 1 20 58 38 Methane monooxygenase Hydroxylase, chain G, domain 1 1 10 238 67 Recoverin, domain 1 1 10 490 32 Globin-like 1 10 287 53 Helix hairpins 1 10 8 40 Helicase, ruva protein, domain 3 1 20 1,050 29 Glutathione S-transferase Yfyf (class Pi), chain A, domain 2 1 20 1,250 27 Growth hormone, chain A 1 10 472 20 Cyclin A, domain 1 1 10 760 33 Cytochrome Bc1 complex, chain D, domain 2 1 20 120 54 Four-helix bundle [hemerythrin (Met), subunit A] 1 10 10 150 Arc repressor mutant, subunit A 1 10 150 54 DNA polymerase, domain 1 1 25 40 67 Serine threonine protein phosphatase 5, tetratricopeptide repeat 1 10 510 28 Transferase (phosphotransferase), domain 1 2 40 50 125 OB fold (dihydrolipoamide acetyltransferase,
    [Show full text]
  • Deep Evolutionary Analysis Reveals the Design Principles of Fold A
    RESEARCH ARTICLE Deep evolutionary analysis reveals the design principles of fold A glycosyltransferases Rahil Taujale1,2, Aarya Venkat3, Liang-Chin Huang1, Zhongliang Zhou4, Wayland Yeung1, Khaled M Rasheed4, Sheng Li4, Arthur S Edison1,2,3, Kelley W Moremen2,3, Natarajan Kannan1,3* 1Institute of Bioinformatics, University of Georgia, Athens, Georgia; 2Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia; 3Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia; 4Department of Computer Science, University of Georgia, Athens, Georgia Abstract Glycosyltransferases (GTs) are prevalent across the tree of life and regulate nearly all aspects of cellular functions. The evolutionary basis for their complex and diverse modes of catalytic functions remain enigmatic. Here, based on deep mining of over half million GT-A fold sequences, we define a minimal core component shared among functionally diverse enzymes. We find that variations in the common core and emergence of hypervariable loops extending from the core contributed to GT-A diversity. We provide a phylogenetic framework relating diverse GT-A fold families for the first time and show that inverting and retaining mechanisms emerged multiple times independently during evolution. Using evolutionary information encoded in primary sequences, we trained a machine learning classifier to predict donor specificity with nearly 90% accuracy and deployed it for the annotation of understudied GTs. Our studies provide an evolutionary framework for investigating complex relationships connecting GT-A fold sequence, structure, function and regulation. *For correspondence: [email protected] Introduction Competing interests: The Complex carbohydrates make up a large bulk of the biomass of any living cell and play essential authors declare that no roles in biological processes ranging from cellular interactions, pathogenesis, immunity, quality con- competing interests exist.
    [Show full text]
  • IDH1 Targeting As a New Potential Option for Intrahepatic Cholangiocarcinoma Treatment—Current State and Future Perspectives
    molecules Review IDH1 Targeting as a New Potential Option for Intrahepatic Cholangiocarcinoma Treatment—Current State and Future Perspectives 1, 1, 1 1 Fabiana Crispo y , Michele Pietrafesa y , Valentina Condelli , Francesca Maddalena , Giuseppina Bruno 2, Annamaria Piscazzi 2, Alessandro Sgambato 1, Franca Esposito 3,* and Matteo Landriscina 1,2,* 1 Laboratory of Pre-Clinical and Translational Research, IRCCS, Referral Cancer Center of Basilicata, 85028 Rionero in Vulture (PZ), Italy; [email protected] (F.C.); [email protected] (M.P.); [email protected] (V.C.); [email protected] (F.M.); [email protected] (A.S.) 2 Medical Oncology Unit, Department of Medical and Surgical Sciences, University of Foggia, 71100 Foggia, Italy; [email protected] (G.B.); [email protected] (A.P.) 3 Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, Italy * Correspondence: [email protected] (F.E.); [email protected] (M.L.); Tel.: +39-081-746-3145 (F.E.); +39-088-173-6426 (M.L.) These authors have contributed equally to this work. y Received: 29 July 2020; Accepted: 17 August 2020; Published: 18 August 2020 Abstract: Cholangiocarcinoma is a primary malignancy of the biliary tract characterized by late and unspecific symptoms, unfavorable prognosis, and few treatment options. The advent of next-generation sequencing has revealed potential targetable or actionable molecular alterations in biliary tumors. Among several identified genetic alterations, the IDH1 mutation is arousing interest due to its role in epigenetic and metabolic remodeling. Indeed, some IDH1 point mutations induce widespread epigenetic alterations by means of a gain-of-function of the enzyme, which becomes able to produce the oncometabolite 2-hydroxyglutarate, with inhibitory activity on α-ketoglutarate-dependent enzymes, such as DNA and histone demethylases.
    [Show full text]
  • Significance of Knotted Structures for Function of Proteins and Nucleic Acids SEPTEMBER 17–21, 2014 | WARSAW, POLAND
    Biophysical Society Thematic Meeting | PROGRAM AND ABSTRACTS Significance of Knotted Structures for Function of Proteins and Nucleic Acids SEPTEMBER 17–21, 2014 | WARSAW, POLAND Ministry of Science and Higher Education Republic of Poland Thank You to the Programming Committee and Local Organizers Program Committee Wilma Olson, Rutgers University, USA Jose Onuchic, Rice University, USA Matthias Rief, Technical University of Munich, Germany Joanna Sulkowska, University of Warsaw, Poland Sarah Woodson, Johns Hopkins University, USA Local Organizers Krsysztof Bryl, University of Warmia and Mazury, Poland Sebastian Kmiecik, University of Warsaw, Poland Andrzej Kolinski, University of Warsaw, Poland Alicja Kurcinska, Secretary, University of Warsaw, Poland Monika Pietrzak, University of Warmia and Mazury, Poland Joanna Sulkowska, University of Warsaw, Poland Mariusz Szabelski, University of Warmia and Mazury, Poland Zbigniew Wieczorek, University of Warmia and Mazury, Poland - 1 - This meeting is financially supported by Ministry of Science and Higher Education University of Warsaw, Center for New Technology (CeNT), Foundation for Polish Science (Inter) and National Science Center Poland (Sonata BIS) Additional support provided by European Molecular Biology Organization (EMBO) Centrum Nowych Technologii - 2 - Significance of Knotted Structures for Function of Proteins and Nucleic Acids Welcome Letter September 2014 Dear Colleagues, We would like to welcome you to the conference on the “Significance of Knotted Structures for Function of Proteins and Nucleic Acids”. During this meeting we will focus on recent progress in identifying a biological role of knots in proteins and nucleic acids. This is a new, interdisciplinary field, which has recently been extensively explored by many groups. One important motivation to study these topics is an increasing number of proteins with knots being discovered.
    [Show full text]
  • Proteins Eugene Shakhnovich Harvard University Boulder, CO July 25-27 2012 Outline
    Proteins Eugene Shakhnovich Harvard University Boulder, CO July 25-27 2012 Outline Lecture 1. Introduction: Principles of structural organization of proteins and Protein Universe. Concepts of convergent and divergent molecular evolution. Lecture 2. Protein folding – Experiment and Theory and Simulations Lecture 3: Bridging scales: From Protein Biophysics to Population Genetics (‘’From Crick to Darwin and back’’) Marriage made in heaven: genomics and protein folding/evolution Proteins are folded on various scales As of now we know hundreds of thousands of sequences (Swissprot) and a few thousand of Structures (protein data bank) Proteins are tightly packed Structural organization of globular proteins Fold, Folding Pattern and Packing Simplified models of protein structures. a), A detailed fold describing positions of secondary structures in the protein hain and in space (see also Fig. 13-1e). (b), The folding pattern of the protein chain with omitted details of loop pathways, the size and exact orientation of α-helices (shown as cylinders) and β-strands (shown as arrows). (c), Packing: a stack of structural segments with no loop shown and omitted details of size, orientation and direction of α-helices and β-strands (which are therefore presented as ribbons rather than as arrows). Similarities at the fold level, differences at the detailed structural level Two close relatives: horse hemoglobin α and horse hemoglobin β (both possessing a heme shown as a wire model with iron in the center). Find similarities and differences. (For tips: they are highly similar although have some differences in details of loop conformations, in details of orientation of some helices, and in one additional helical turn available in the β globin, on the right).
    [Show full text]
  • Bhanupratap Singh Chouhan – Integrin Evolution
    Bhanupratap Singh Chouhan | Integrin evolution: From prokaryotes | 2016 within chordates the diversification to Bhanupratap Singh Chouhan | Integrin From evolution: Bhanupratap Singh Chouhan Integrin evolution: from prokaryotes to the diversification within chordates 9 789521 234484 ISBN 978-952-12-3448-4 Bhanupratap Singh Chouhan was born on September 04,1985 in Indore, M.P., India. He did his Bache- lor of Science in Bioinformatics from Government Autonomous Holkar Science College, Devi Ahilya University in Indore. He then graduated from the University of Turku in 2009 with a Master of Science in Bioinformatics. This Ph.D. thesis work has taken place under the supervision of Professor Mark S. Johnson and Docent Konstantin Denessiouk at the Structural Bioinformatics Laboratory (SBL), Åbo Akademi University, Turku, Finland during 2010-2015. Integrin evolution: From prokaryotes to the diversification within chordates Bhanupratap Singh Chouhan Biochemistry Faculty of Science and Engineering Åbo Akademi University Åbo, Finland 2016 Supervised by: Professor Mark S. Johnson Docent Konstantin Denessiouk Dr. Alexander Denesyuk Åbo Akademi University Åbo, Finland Reviewed by: Professor Donald Gullberg, University of Bergen, Norway. Docent Janne Ravantti, University of Helsinki, Finland. Opponent: Docent Olli Pentikäinen, University of Jyväskylä, Finland. ISBN 978-952-12-3448-4 Painosalama Oy – Turku, Finland 2016 Table of contents 1. Review of the literature 1 1.1 Introduction 1 1.2 Integrin bidirectional signaling 6 1.3 Relationship among integrin α and β subunits 12 1.4 N-terminal region: The β-propeller domain 15 1.5 N-terminal region: The vWA domain 17 1.6 Collagens and vertebrate collagen receptors 19 1.7 Structure of collagen-binding and ICAM-binding integrins 21 2.
    [Show full text]