Genetics: Published Articles Ahead of Print, published on October 22, 2006 as 10.1534/genetics.106.065102 Multilocus patterns of nucleotide diversity, linkage disequilibrium and demographic history of Norway spruce (Picea abies (L.) Karst). Myriam Heuertz*,¶,§,1, Emanuele De Paoli†,§, Thomas Källman*,§, Hanna Larsson*, Irena Jurman†, Michele Morgante†, Martin Lascoux* and Niclas Gyllenstrand*,§ * Program in Evolutionary Functional Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, 75326 Uppsala Sweden ¶Centre de Recherche Public-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Luxembourg † Dipartimento di Scienze Agrarie ed Ambientali, Università di Udine, Via delle Scienze 208, 33100 Udine , Italy 1Current address: Laboratoire d’Eco-Ethologie Evolutive - CP 160/12, Université Libre de Bruxelles. 50, Av. F.D. Roosevelt, B-1050 Bruxelles, Belgium §These authors contributed equally to the present study. Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AM267499 - AM268023, AM268895 - AM269360 1 Keywords: Norway spruce, coalescent, linkage disequilibrium, bottleneck short running head: DNA Polymorphism in Norway spruce Corresponding author: Martin Lascoux, Program in Evolutionary Functional Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, 75326 Uppsala Sweden Email:
[email protected] 2 ABSTRACT DNA polymorphism at 22 loci was studied in an average of 47 Norway spruce (Picea abies (L.) Karst.) haplotypes sampled in 7 populations representative of the natural range. The overall nucleotide variation was limited, being lower than that observed in most plant species so far studied. Linkage disequilibrium was also restricted and did not extend beyond a few hundred base pairs. All populations, with the exception of the Romanian population, could be divided into two main domains, a Baltico-Nordic and an Alpine one.