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Supplementary Material Localizing Regions in the Genome

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Supplementary Material Localizing Regions in the Genome Supplementary Material Localizing regions in the genome contributing to ADHD, aggressive and antisocial behavior Running title: Genetic overlap between ADHD, aggression and antisocial behavior Mariana Lizbeth Rodríguez López1, Barbara Franke1,2*, Marieke Klein1,3 1 Radboud university medical center, Donders Institute for Brain, Cognition and Behaviour, Department of Human Genetics, Nijmegen, The Netherlands 2 Radboud university medical center, Donders Institute for Brain, Cognition and Behaviour, Department of Psychiatry, Nijmegen, The Netherlands 3 University Medical Center Utrecht, UMC Utrecht Brain Center, Department of Psychiatry, Utrecht, the Netherlands Supplementary Tables: 5 Supplementary Figures: 2 Supplementary Table 1 | Category traits from LDHub GWAS-ss database. Category Number of traits Aging 3 Anthropometric 22 Autoimmune 11 Bone 5 Brain Volume 7 Cancer 5 Cardiometabolic 2 Cognitive 1 Education 5 Glycemic 8 Haemotological 3 Hormone 2 Kidney 6 Lipids 4 Lung Function 8 Metabolites 107 Metal 2 Neurological 3 Other 1 Personality 4 Psychiatric 11 Reproductive 4 Sleeping 5 Smoking 4 Behaviour Uric Acid 1 Total 234 A list of all categories from all the traits LDHub platform. We performed genetic correlation analyses for all traits with both AGG and ASB, giving a total of 234 rg scores for each one of our two traits. Supplementary Table 2 | Summary of data from GTEx project (https://gtexportal.org/home/). GTEx - Gene expression in 12 brain-related tissues Anterior Caudate Frontal cingulate (basal Cerebellar Cortex Nucleus Substantia Tissues Amygdala cortex ganglial) Hemisphere Cerebellum Cortex (BA9) Hippocampus Hypothalamus accumbens Putamen nigra Group 1 PBXIP1 83.295 92.11 136.3 42.495 50.13 86.42 72.98 51.38 83.98 135.9 95.535 103.7 PYGO2 19.865 23.33 22.93 58.905** 57.1** 30.665 30.97 19.49 28.93 26.55 18.26 20.235 KCNT2 1.2525 1.856 0.8475 0.3605 0.85 2.4595 3.221* 1.539 2.246 1.142 0.7494 1.1555 CFHR1 0.03493 0.0595 0.016485 0 0 0.029725 0.03561 0.05782 0.1552 0.01877 0.01452 0.09924 F13B 0 0 0 0 0 0 0 0 0 0 0 0 ZBTB41 4.0555 4.681 4.0645 21.005** 19.24** 5.2035 7.079 4.327 5.938 4.999 3.072 4.5755 ZC3H8 3.987 5.256 4.829 16.335** 12.05** 5.8525 7.851* 4.174 5.688 6.062 3.81 4.5175 ZC3H6 2.258 2.435 2.835 9.2065** 8.476* 3.323 3.629 2.639 2.979 3.42 2.303 2.648 TTL 6.8825 6.923 7.939 6.637 6.429 8.809** 9.624** 8.117* 6.115 8.315* 8.3165* 7.1635 TBC1D14 11.735 13.8 12.445 12.505 14.12 14.94 15.41 10.51 12.38 15.09 8.727 11.15 C5orf51 8.315 9.273 9.3295 23.25** 17.05 8.183 12.66 9.085 11.75 10.41 7.2935 10.67 FBXO4 2.5295 2.412 2.405 5.5165 4.776 2.481 2.712 2.412 3.399 2.987 2.1895 2.682 GHR 0.6335 0.8804 0.6436 0.3533 0.4992 0.77815 0.9152 0.6902 1.715 0.8455 0.507 1.1485 CCDC152 3.024 1.502 2.888 0.77565 0.9818 1.872 1.943 2.848 4.652 2.928 2.261 3.594 SEPP1 25.305 13.02 34.605 11.75 21.34 17.61 17.42 38.15 27.07 23.3 31.23 69.42 ZNF131 4.107 4.919 4.843 20.61** 16.16** 5.59 7.157 4.49 5.125 5.408 3.692 4.4385 HMGCS1 26.14 38.67 17.27 37.37 30.48 43.64 62.84* 25 44.03 22.86 13.29 21.655 NKAIN2 16.775* 15.88* 9.8155 13.125* 8.768 10.87* 17.97** 23.25** 13.4* 9.397 9.199 23.99** GATA4 0.01081 0.009618 0.01293 0.014995 0.02724 0.014875 0.01265 0.01436 0.01566 0.01179 0.010335 0.01081 TMEM71 0.38965 0.3923 0.443 0.2385 0.234 0.3438 0.3669 0.4123 0.584 0.3732 0.43025 0.47885 TG 1.02 0.5413 0.4633 0.5515 0.717 0.541 0.4967 0.7798 0.4466 0.4407 0.3571 0.4067 SLA 2.6845 2.208 2.0815 1.2075 1.307 1.253 1.376 2.968 2.232 2.725 1.494 3.165 CHUK 4.0345 3.989 4.9745 10.76* 10.03 3.918 4.755 4.255 6.679 4.882 4.2505 5.2315 CWF19L1 4.8505 6.225 5.869 21.69** 18.36* 8.19 9.456 5.533 7.615 7.45 5.029 6.0695 BLOC1S2 35.795 42.05* 28.775 49.665** 39.27 40.46 58.26** 33.11 48.46** 35.55 26.53 45.475* ATRNL1 5.4335* 10.27** 4.1875 0.85805 2.225 18.255** 25.43** 4.859 3.722 5.022 3.125 1.903 KCNK18 0 0.0276* 0.16495** 0 0 0.034725* 0.05654** 0 0.07626** 0.1824** 0.03221* 0 TMEM132B 7.937* 12.46** 8.6615* 1.7025 1.323 14.795** 17.8** 7.022* 4.371* 11.73** 6.917* 3.3505 MLLT6 33.17 41.72 27.875 96.45* 103.6* 52.765 46.42 28.87 26.22 31.24 24.98 25.86 PCGF2 12.515 16.2 12.455 54.53 54.1 21.69 20.86 11.7 18.3 15.2 11.02 10.7 ANKRD54 13.155 15.42 12.6 32.335** 32.58** 17.85 18.08 12.63 18.47 13.93 11.08 12.03 TMEM184B 33.285 47.09 30.875 58.175* 76.82** 66.08** 57.05 36.68 40.53 34.47 29.73 36.585 GTPBP1 12.1 13.6 12.655 39.09* 41.08** 18.73 17.15 13.16 14.21 15.6 10.955 12.095 SUN2 36.555 22.13 47.785 48.02 55.66 40.12 35.58 49.63 32.79 30.4 46.46 46.79 Group 2 DAB1 3.487* 4.671* 1.1335 24.035** 18.68 ** 3.73* 4.35* 4.86** 3.439* 1.432 0.8718 2.5095 F13B 0 0 0 0 0 0 0 0 0 0 0 0 RBM43 1.7745 1.542 2.121 0.7295 0.833 1.354 1.712 1.418 2.189 2.418 1.4555 1.7525 NMI 3.3705 2.543 3.1935 1.1825 1.421 2.403 2.298 2.922 3.216 2.854 2.817 3.987 SHOX2 0.01234 0.01641 0.010064 0.02929 0.02808 0.023865 0.0188 0.009096 0.136 0.02091 0.010615 0.074495 RSRC1 4.481 5.631 5.3415 17.26** 11.09** 5.625 9.453* 4.501 6.478 6.062 3.7855 5.326 HMGCS1 26.14 38.67 17.27 37.37 30.48 43.64 62.84* 25 44.03 22.86 13.29 21.655 CCL28 0.6247 0.6295 0.3163 0.100535 0.144 0.86005 1.027 0.4091 0.625 0.2951 0.174 0.4703 MSRA 6.2405 8.598 6.1115** 29.7** 28.09 10.08 11.05* 7.369 5.962 6.695 4.88 6.181 PRSS55 0.1154 0.1309 0.11825 0.57605 0.5789 0.14815 0.141 0.1179 0.05924 0.1043 0.11545 0.08649 FAM167A 14.395* 19.29** 14.09* 7.613 12.58 19.525** 16.77* 9.731 11.33 12.71 11.785 10.905 BLK 0.01749 0.01973 0.016935 0.08194 0.1098 0.026865 0.02019 0.03163 0.04851 0.01234 0.0171 0.035155 GATA4 0.01081 0.009618 0.01293 0.014995 0.02724 0.014875 0.01265 0.01436 0.01566 0.01179 0.010335 0.01081 NEIL2 20.405 21.24 18.465 28.14** 26.35* 24.005 27.17* 17.57 28.91** 24.13* 15.95 22.915 FDFT1 30.84 34.31 24.1 39.735 37.45 41.945 49.73 31.45 43.87 27.17 20.88 35.09 CTSB 65.295 89.44 88.39 104.6 93.59 119.65 142.7 68.3 104.2 91.24 70.485 98.18 DEFB136 0 0 0 0 0 0 0 0 0 0 0 0 DEFB134 0.075625 0.06506 0.11595 0.5516 0.5019 0.097625 0.08884 0.02926 0.08835 0.09981 0.058605 0 USP17L7 0 0.02502 0.04138 0.07753 0.1282 0.027565 0.02539 0.01203 0 0.03694 0.02931 0 KIAA1456 1.475 3.078 2.946 105.9** 92.84** 3.525* 4.485** 2.366 2.776 3.115* 1.705 1.6125 NOX4 0.1155 0.06863 0.15025 0.036925 0.04916 0.11765 0.1186 0.1321 0.159 0.09159 0.1528 0.2001 LRP1 0 0 0 0 0 0 0 0 0.02956 0 0 0.016335 NXPH4 1.5055 1.937 0.7042 28.445** 31.54** 1.563 1.725 0.9111 2.86* 0.4112 0.7665 1.0036 STAC3 1.6805 1.695 1.842 4.2185 4.353 1.7835 1.701 1.769 1.781 1.713 1.7585 1.8685 R3HDM2 21.13 27.33 21.83 83.45** 80.54** 42.855 43.62* 20.9 23.26 25.5 18.29 20.485 INHBC 0.06522 0.07656 0.059665 0.6836 0.7682 0.097185 0.09135 0.04705 0.0478 0.08805 0.065755 0.0623 INHBE 0.115 0.2444 0.1075 1.2605 1.29 0.2567 0.2883 0.07665 0.2749 0.2486 0.06807 0.2074 ARHGEF40 13.52 12.13 13.145 31.395 35.83 13.22 9.816 11.27 15.91 13.19 11.51 14.32 ATP5G1 80.29 98.44 94.955 100.7 88.54 99.605 132.3** 80.51 87.83 108.9* 81.8 81.525 UBE2Z 24.55 30.01 22.435 53.51 47.08 39.355 45.63 27.55 33.21 29.55 19.425 25.575 SNF8 14.515 17.22 16.65 27.275 24.38 19.185 21.23 14.52 17.62 18.5 15.22 17.215 GIP 0 0 0 0 0 0 0 0 0 0 0 0 C19orf66 25.295 31.29 29.63 54.805 57.75 45.24 41.33 25.97 37.12 31.77 25.76 21.31 BCL3 3.375 3.374 2.8825 1.7795 2.944 5.2995 4.1 2.908 5.248 2.613 2.266 5.2565 KLC3 0.07445 0.2325 0.40385 0.045445 0.05787 0.2108 0.2143 0.07716 0.1107 0.5094 0.2935 0.07667 ERCC2 8.8075 10.89 8.445 14.18* 16.12* 14.135* 12.93 7.96 9.207 10.52 7.2585 7.3945 IGLON5 12.49* 20.93* 6.529 127.8** 131.6** 27.85** 28.63** 15.6* 32.96** 9.206 6.0315 19.79* VSIG10L 1.728 2.634 1.8855 7.122 6.86 2.5915 3.004 2.167 3.497 2.411 1.4695 3.37 ETFB 24.075 26.57 28.12 21.525 20.28 25.56 28.38 24.41 24.93 33.8* 27.555 32.19 Group 3 SRSF10 1.36 1.55 1.812 5.4905 4.688 1.585 1.983 1.522 1.928 1.863 1.2575 1.666 RCAN3 1.331 1.176 2.1395 59.835** 39.96** 1.072 1.386 1.719 7.823 2.149 2.1295 4.5765 Results show the median Transcripts Per Million (TPM) for each gene from the candidate loci across 12 brain-related tissues.
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