Improvement of the Nutritional Value of a Cereal Fermented Milk: 2-Dried Kishk Like
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cess Pro ing d & o o T F e c f h Nassar et al., J Food Process Technol 2016, 7:11 o n l o a l n o r Journal of Food DOI: 10.4172/2157-7110.1000638 g u y o J Processing & Technology ISSN: 2157-7110 Research Article Open Access Improvement of the Nutritional Value of a Cereal Fermented Milk: 2-Dried Kishk Like Nassar KS1*, Shamsia SM1 and Attia IA2 1Department of Food and Dairy Science and Technology, Damanhour University, Egypt 2Department of Dairy Science and Technology, Alexandria University, Egypt Abstract The present study has been conducted to produce fermented milk fortified with different cereals like whole wheat, barley and freek (green wheat) burghul have been selected for their known nutrition benefits. The fermentation was occurred by using three types of cultures, yogurt starter, yogurt starter + Bio-yogurt or yogurt starter + Lactobacillus plantarum. All samples were stored at room temperature (25 ± 2°C) for three months and have been subjected to consumer sensory testing; dried kishk-like products were highly accepted by the tasting panel, furthermore fermented dairy products containing Freek gained the highest score of judging followed by wheat. Proximate composition, Colour, Organic acids and microbiologically analysis have been monitored in the fresh soft product and during storage. Nevertheless, the combined levels of organic acids, low pH, salt additive and low moisture content in the kishk samples were sufficient to ensure the microbial safety of the product. Thus, long shelf-life of all samples without changing in their chemical during the storage period has been noticed. Keywords: Burghul; Probiotic bacteria; Dried kishk; Organic acids; paste was filled in polystyrene cups and covered then incubated at Colour; Skim milk (43°C for W1, B1 and F1) and (37 C for W2, W3, B2, B3, F2 and F3) to 6 hours (Table 1). After that, the○ fermented paste was formed into Introduction nuggets (3-5 cm), placed into stainless steel trays and dried in air oven Fermentation is one of the oldest and most economical at 50 C for 15-18 h. the dried nuggets were milled by using a hammer ○ methods of producing and preserving food. Also, leads to a general mill. After that, the dried kishk were stored in airtight glass containers improvement in the shelf life, texture, taste and aroma of the final and kept at room temperature until tested (Figure 1). product. Fermented foods are produced world-wide using various Chemical analysis manufacturing techniques, raw materials and microorganisms [1]. Kishk is an extremely popular fermented food in many parts of the In the previous paper, the same methods were adopted for cereal Middle East. In Egypt, Kishk is one of the traditional food products and dairy base analysis as described in Nassar et al. [9]. in Upper Egypt [2,3]. There are some other products similar to kishk Dried Kishk-like analysis such as tarhana (mixing yogurt, wheat flour, baker's yeast and variety of vegetables and spices in turkey), kushuk (milk- sour dough mixture Proximate composition: Total solids, protein, ash and fiber with turnips in Iraq), atole (fermented cereal–milk porridge in Scotland content were determined according to procedures described by and Greece) and tahonya/talkuna (fermented cereal mixture with AOAC [10]. The fat content, salt percentage, acidity and pH value vegetables in Finland and Hungary) [4]. Kishk is a natural, healthy, were determined according to Ling [11]. Finally, carbohydrate was respect the environment and have great taste and cultural values that calculated as follows [12]: are increasingly attractive to the Egyptian consumers. Kishk made by Carbohydrate = total solids - (protein + fat + ash) mixing wheat with fermented milk (Laban zeer) and sun-drying the mixture to 8% to 12% moisture content [2,5] or made from different Colour of kishk-like samples: Colour of dried kishk samples cereal products and fermented milk base by traditional methods of was evaluated by Lovibond Schofield Tintometer (the Tintometer manufacture [6,7]. Kishk is usually reconstituted with water and served Ltd. Salisbury, England). Colours of samples were assessed. Reading as a hot gruel, but with the incorporation of vegetables, spices, garlic or obtained was further converted into C.I.E. (Commission International herbs, can form the base of savory and sweet dishes [8]. The aim of the de L'E Clairage) units using the visual density graphs and direction present study was to focus some light on the chemical, biochemical, booklet supplied with apparatus as described in AOCS [13]. physical and sensory aspects of dried fermented milk made from various types of cereal and starter cultures. *Corresponding author: Khaled S Nassar, Department of Food and Dairy Materials and Methods Science and Technology, Damanhour University, Egypt, Tel: +20-453368069; In the previous paper, the preparation kishk-like from reconstituted E-mail: [email protected] skim milk and burghul from whole wheat, whole barley and fereek was Received November 04, 2016; Accepted November 22, 2016; Published described in Nassar et al. [9]. November 29, 2016 Citation: Nassar KS, Shamsia SM, Attia IA (2016) Improvement of the Nutritional Dried Kishk-like manufacturing Value of a Cereal Fermented Milk: 2-Dried Kishk Like. J Food Process Technol 7: 638. doi: 10.4172/2157-7110.1000638 Each type of burghul was mixed with reconstituted skim milk in a ratio of 1:4 (w/w) in addition to, 2% sodium chloride and then mixed Copyright: © 2016 Nassar KS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits thoroughly each 26%. Mixture was heated to 95°C for 10 seconds, and unrestricted use, distribution, and reproduction in any medium, provided the then rapidly cooled to 45°C, addition 3% of each Starter. The resultant original author and source are credited. J Food Process Technol, an open access journal Volume 7 • Issue 11 • 1000638 ISSN: 2157-7110 Citation: Nassar KS, Shamsia SM, Attia IA (2016) Improvement of the Nutritional Value of a Cereal Fermented Milk: 2-Dried Kishk Like. J Food Process Technol 7: 638. doi: 10.4172/2157-7110.1000638 Page 2 of 7 Cereals Dairy base Salt 2% Treatments Whole Wheat Burghul (W) Whole Barley Burghul (B) Freek Burghul (F) Re-constituted Skim milk (15%) √ √ √ √ √ √ 1 √ √ √ √ √ √ √ √ √ 2 √ √ √ √ √ √ √ √ √ 3 √ √ √ 1: (3% Yoghurt starter) 2: (2% Yoghurt starter + 1% Bio-yoghurt starter) 3: (2% Yoghurt starter + 1% Lactobacillus plantarum) Table 1: Experimental treatments. Reconstituted skim milk (15 W/W) Wheat Burghul Barley Burghul Freek Burghul Mixture (1:4 w/w) (cereal/dairy Base) + 2% sodium chloride Heat treatment at 95°C/10 Sec cooled to 45°C Addition of different Starter cultures (3%) Filling in plastic cups Incubation at suitable temperature until desired pH Cooling to room temperature Shaped into small balls and spread into stainless steel trays Drying at 60°C for 15-18 hrs in an air oven Milled the dried kishk by using a hammer mill Packed and stored in airtight glass containers at room temperature until used. Figure 1: Dried kishk-like preparation by using different substrates and starters. Organic acid determination: The concentrations of organic acids Sensory evaluation (Lactic, Propionic, acetic, and formic) in different dried Kishk samples were determined by HPLC (Spectra-Physics system, San Jose, CA, Organoleptic evaluation was carried out according to Abou- USA) method as described by Barrantes et al. [14]. Organic acids were Donia et al. [16]. The samples were subjected to organoleptic analysis extracted from dried Kishk (5 g) in a 50 mL beaker using 25 mL water- by 15 well-trained members of the Dairy Science and Technology acetonitrile (1:4 v/v) (analytical grade, BDH Chemicals Ltd., Poole, Department (Fac. Agric. Alexandria Univ., Egypt). The sensory UK). The extract after filtration through a Whatman No. 1 filter paper attributes evaluated were: The Flavor (1-45 points), Body and Texture (Whatman Ltd., Maidstone, UK) was injected (20 μL) into the HPLC (1-30 points), appearance and Colour (1-15 points) and acidity (1- 10 column. The flow rate of the solvent was 0.7 μL.min-1 at 65°C and the points). Soups were prepared by adding 20 g dried kishk to 170 mL of wave length of the detector was 220 nm [15]. water and heating with gentle stirring to boiling, simmering for few J Food Process Technol, an open access journal Volume 7 • Issue 11 • 1000638 ISSN: 2157-7110 Citation: Nassar KS, Shamsia SM, Attia IA (2016) Improvement of the Nutritional Value of a Cereal Fermented Milk: 2-Dried Kishk Like. J Food Process Technol 7: 638. doi: 10.4172/2157-7110.1000638 Page 3 of 7 min and cooling to 40°C. The samples (20 g) were placed in identical and multiple comparisons of means of each treatment (cereals, starter glass containers (8 cm diameter, 3.5 cm height) and served at 40°C [17]. cultures and storage time) using the Least Significant Difference (LSD) test at the confidence level of 95% [24]. Enumeration of microorganisms Results and Discussion The counts of lactic acid bacteria were enumerated as (CFU/g) using MRS agar according to De man et al. [18]. Proteolytic bacteria, Proximate composition coliforms, yeast and mould were enumerated respectively [19-21]. Whereas aerobic spore forming bacteria were enumerated according Proximate analysis of kishk-like products is presented in Tables to the method described by Harrigan and McCance [22]. Lactobacillus 2-4. The results revealed that the effect of cereal type on the proximate acidophilus was enumerated according to the method described by analysis of the resultant dried kishk-like products was more pronounced Lapierra [23]. (P ≤ 0.05) than that of type of starter culture used.