Conservation Genetics (2005) 6:511–525 Ó Springer 2005 DOI 10.1007/s10592-005-9006-8 Genetic management of the red squirrel, Sciurus vulgaris: a practical approach to regional conservation Rob Ogden1,*, Craig Shuttleworth2, Ross McEwing1 & Silvana Cesarini3 1Wildlife DNA Services, 9th Floor Alun Roberts Building, University of Wales, Bangor, LL57 2UW, UK; 2Menter Moˆn, Llys Goferydd, Staˆd Ddiwydiannol Bryn Cefni, Llangefni, Ynys Moˆn, LL77 7XA, UK; 3Cardiff School of Biosciences, Cardiff University, P.O. Box 915, Cardiff, CF10 3TL, UK (*Corresponding author: phone: +44-(0)-1248-388479; fax: +44-(0)-1248-388484; e-mail:
[email protected]) Received 15 April 2004; accepted 5 August 2004 Key words: genetic diversity, native species, population fragmentation, reintroduction Abstract The progressive decline in red squirrel (Sciurus vulgaris) numbers in Wales has led to conservation and reintroduction projects being established on the island of Anglesey. The recovery of the island’s remnant wild population was initially successful, however concern remained over potential loss of genetic diversity resulting from an observed demographic bottleneck. We used mitochondrial DNA (mtDNA) control region sequences and six microsatellite loci to assess current levels of genetic variation in the population. Samples were monomorphic for control region sequences and a historic specimen from the same area carrying a different haplotype demonstrated a loss of mtDNA diversity during the last 20 years. Inclusion of other Welsh haplotypes indicated phylogeographic structure in the region, in contrast to previous UK studies. Genotyping results showed allelic diversity and heterozygosity to be less than 50% of that re- corded in other UK populations, with strong evidence for a recent genetic bottleneck.