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IFMBE Proceedings IFMBE Proceedings Volume 53 Series Editor Ratko Magjarevic Deputy Editors Fatimah Binti Ibrahim Igor Lackovic´ Piotr Ładyzy˙ nski´ Emilio Sacristan Rock The International Federation for Medical and Biological Engineering, IFMBE, is a federation of national and transnational organizations representing internationally the interests of medical and biological engineering and sciences. The IFMBE is a non-profit organization fostering the creation, dissemination and application of medical and biological engineering knowledge and the management of technology for improved health and quality of life. Its activities include participation in the formulation of public policy and the dissemination of information through publications and forums. Within the field of medical, clinical, and biological engineering, IFMBE’s aims are to encourage research and the application of knowledge, and to disseminate information and promote collaboration. The objectives of the IFMBE are scientific, technological, literary, and educational. The IFMBE is a WHO accredited NGO covering the full range of biomedical and clinical engineering, healthcare, healthcare technology and management. It is representing through its 60 member societies some 120.000 professionals involved in the various issues of improved health and health care delivery. IFMBE Officers President: Ratko Magjarevic, Vice-President: James Goh Past-President: Herbert Voigt Treasurer: Marc Nyssen, Secretary-General: Shankhar M. Krishnan http://www.ifmbe.org More information about this series at http://www.springer.com/series/7403 Tomaž Jarm · Peter Kramar Editors 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies (WC 2015) Portorož, Slovenia, September 6–10, 2015 ABC Editors Tomaž Jarm Peter Kramar University of Ljubljana University of Ljubljana Faculty of Electrical Engineering Faculty of Electrical Engineering Ljubljana Ljubljana Slovenia Slovenia ISSN 1680-0737 ISSN 1433-9277 (electronic) IFMBE Proceedings ISBN 978-981-287-816-8 ISBN978-981-287-817-5 (eBook) DOI 10.1007/978-981-287-817-5 Library of Congress Control Number: 2015949816 Springer Singapore Heidelberg New York Dordrecht London c Springer Science+Business Media Singapore 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein orforany errors or omissions that may have been made. The IFMBE Proceedings is an Official Publication of the International Federation for Medical and Biological Engineering (IFMBE) Printed on acid-free paper Springer Science+Business Media Singapore Pte Ltd. is part of Springer Science+Business Media (www.springer.com) Preface Welcome to the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies (WC2015), the first scientific congress with the goal to bring together researchers and users of electroporation-based and related technologies from academic and medical institutions and the industry. This meeting presents an excellent opportunity to meet with people from different disciplines possessing diverse expertise, who are involved in basic or applied research or are developing applications based on electroporation and the use of pulsed electric fields of high intensity. The aim of the WC2015 is to create an environment stimulating interdisciplinary interactions, to present latest achievements and to demonstrate current knowledge. To achieve this, the Congress will consist of plenary sessions given by distinguished invited lecturers, poster-viewing sessions, and parallel scientific sessions, interspersed with generous coffee and lunch breaks to foster lively discussion and exchange of ideas. More than 360 scientific contributions will be presented over four days by authors from nearly 50 countries from all six continents. The following main areas will be covered: • Application of pulsed electric fields technology in food: challenges and opportunities • Electrical impedance measurement for assessment of electroporation yield • Electrochemistry and electroporation • Electroporation meets electrostimulation • Electrotechnologies for food and biomass treatment • Food and biotechnology applications • In vitro electroporation - basic mechanisms • Interfacial behaviour of lipid-assemblies, membranes and cells in electric fields • Irreversible electroporation in clinical use • Medical applications: electrochemotherapy • Medical applications: gene therapy • Non-electric field-based physical methods inducing cell poration and enhanced molecule transfer • Non-thermal plasmas for food safety, environmental applications and medical treatments • PEF for the food industry: fundamentals and applications • PEF process integration - complex process chains and process combinations in the food industry • Predictable animal models • Pulsed electric fields and electroporation technologies in bioeconomy • Veterinary medical applications The WC2015 is also dedicated to encouraging young researchers to develop their skills and expertise in electroporation- related areas through the Young Investigator Competition. YIC is organised in three categories: Biomedical Engineer- ing (sponsored by IFMBE - the International Federation for Medical and Biological Engineering); Food, Biotechnology and Environment (sponsored by DIL - German Institute of Food Technologies); and Medicine and Biology (sponsored by Frank Reidy). Two satellite events will be incorporated in the WC2015, namely BFE2015 - the 3rd International Bio & Food Electrotech- nologies Symposium; and Bioelectrics 2015 - The 12th International Bioelectrics Symposium. WC2015 is organized by COST TD1104 Action (European network for development of electroporation-based technologies and treatments). COST TD1104 Action is a large international consortium connecting 569 researchers from 235 institutions and 42 countries. For more information please visit the COST TD1104 Action’s website at www.electroporation.net. For more information about the Congress visit wc2015.electroporation.net. We hope that you will enjoy the WC2015 and we wish you a pleasant stay in Portorož Damijan Miklavciˇ cˇ The Chair of the COST TD1104 Action On behalf of the International Organising Committee and the Scientific Programme Committeee Satellite Events Incorporated in WC2015 BFE2015 The 3rd International Bio & Food Electrotechnologies Symposium Bioelectrics 2015 The 12th International Bioelectrics Symposium 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies (WC2015) September 6-10, 2015 Portorož, Slovenia Organisation Committees International Organising Committee Volker Heinz, Germany LluisM.Mir,France Richard Heller, USA Javier Raso, Spain Damijan Miklavciˇ c,ˇ Slovenia Scientific Advisory Board Gustavo V. Barbosa-Canovas, USA Boris Rubinsky, USA Dietrich Knorr, Germany Justin Teissié, France Eberhard Neumann, Germany Karl H. Schoenbach, USA Carlo Ricardo Rossi, Italy James C. Weaver, USA Scientific Programme Committee Hidenori Akiyama, Japan Guillermo Marshall, Argentina Sid Becker, New Zealand Olga Martín-Belloso, Spain Roman Buckow, Australia Mihaela G. Moisescu, Romania Maja Cemažar,ˇ Slovenia Marie-Pierre Rols, France Rafael V. Davalos, USA Gregor Serša, Slovenia David A. Dean, USA Declan Soden, Ireland Giovanna Ferrari, Italy Mounir Tarek, France Wolfgang Frey, Germany Stefan Töpfl, Germany Julie Gehl, Denmark P. Thomas Vernier, USA Mark J. Jaroszeski, USA Eugène Vorobiev, France Hao Lin, USA C.Y. Xiao, China James Lyng, Ireland Ken-Ichi Yano, Japan Organising Committee (University of Ljubljana) Tomaž Jarm, Slovenia Tadeja Forjanic,ˇ Slovenia Peter Kramar, Slovenia Saša Haberl Meglic,ˇ Slovenia Samo Mahnic-Kalamiza,ˇ Slovenia Alenka Macekˇ Lebar, Slovenia Damijan Miklavciˇ c,ˇ Slovenia Bor Kos, Slovenia Matej Kranjc, Slovenia and Lea Rems, Slovenia Tina Batista Napotnik, Slovenia Barbara Zorec, Slovenia Janja Dermol, Slovenia XII Committees Young Investigator Competition Committee Nikolai Lebovka, Ukraine Justin Teissié, France James Lyng, Ireland P. Thomas Vernier, USA Boris Rubinsky, USA Scientific Reviewers Oliver Bals, France Orjan Martinsen, Norway Farid Chemat, France Olga Martín-Belloso, Spain Maja Cemažar,ˇ Slovenia Damijan Miklavciˇ c,ˇ Slovenia Maria Laura Fernandez, Argentina Sea Min, Korea Giovanna Ferrari, Italy LluisM.Mir,France Wolfgang Frey, Germany Thomas Nacke, Germany Alexander Golberg, Israel Andrew Nelson, UK Muriel Golzio, France Gianpiero Pataro, Italy Nabil Grimi, France Uwe Pliquett, Germany Richard
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