Jian-Xin Qi, Ming-Sheng Zhu & Wilson R. Lourenço
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Studies on the Hyaluronidase Enzyme Purified from the Venom of Chinese Red Scorpion Buthus Martensi Karsch
STUDIES ON THE HYALURONIDASE ENZYME PURIFIED FROM THE VENOM OF CHINESE RED SCORPION BUTHUS MARTENSI KARSCH FENG LUO NATIONAL UNIVERSITY OF SINGAPORE 2010 STUDIES ON THE HYALURONIDASE ENZYME PURIFIED FROM THE VENOM OF CHINESE RED SCORPION BUTHUS MARTENSI KARSCH A thesis submitted by FENG LUO (B.Med., M.Med.) for the degree of DOCTOR OF PHILOSOPHY in the NATIONAL UNIVERSITY OF SINGAPORE Department of Anatomy Yong Loo Lin School of Medicine National University of Singapore 2010 Acknowledgements I would like to take this opportunity to express my sincere appreciation to my supervisor Prof. P. Gopalakrishnakone, Department of Anatomy, National University of Singapore. During my study in Anatomy, I owed much to his great patience, academic guidance and endlessly encouragement. It is my luck to study under his supervision. I’d also like to thank Prof. Bay Boon Huat, the head of Department of Anatomy, National University of Singapore, for his management to make the whole department as a big family and hence I could enjoy the stay in the department. I will not forget the great support from Dr. Gao Rong. I have learned much from him, from the understanding of the science to the techniques of the experiments. I felt so happy to meet such a big brother in the lab. I would also thank Dr. M.M.Thwin, the senior member of Venom and Toxin Research Programme, who helped me in writing the manuscript, patiently listened to my queries and unselfishly shared his experience. He was always available when I needed the help. I highly appreciate the kindly help of Mr. -
Honeybee (Apis Mellifera) and Bumblebee (Bombus Terrestris) Venom: Analysis and Immunological Importance of the Proteome
Department of Physiology (WE15) Laboratory of Zoophysiology Honeybee (Apis mellifera) and bumblebee (Bombus terrestris) venom: analysis and immunological importance of the proteome Het gif van de honingbij (Apis mellifera) en de aardhommel (Bombus terrestris): analyse en immunologisch belang van het proteoom Matthias Van Vaerenbergh Ghent University, 2013 Thesis submitted to obtain the academic degree of Doctor in Science: Biochemistry and Biotechnology Proefschrift voorgelegd tot het behalen van de graad van Doctor in de Wetenschappen, Biochemie en Biotechnologie Supervisors: Promotor: Prof. Dr. Dirk C. de Graaf Laboratory of Zoophysiology Department of Physiology Faculty of Sciences Ghent University Co-promotor: Prof. Dr. Bart Devreese Laboratory for Protein Biochemistry and Biomolecular Engineering Department of Biochemistry and Microbiology Faculty of Sciences Ghent University Reading Committee: Prof. Dr. Geert Baggerman (University of Antwerp) Dr. Simon Blank (University of Hamburg) Prof. Dr. Bart Braeckman (Ghent University) Prof. Dr. Didier Ebo (University of Antwerp) Examination Committee: Prof. Dr. Johan Grooten (Ghent University, chairman) Prof. Dr. Dirk C. de Graaf (Ghent University, promotor) Prof. Dr. Bart Devreese (Ghent University, co-promotor) Prof. Dr. Geert Baggerman (University of Antwerp) Dr. Simon Blank (University of Hamburg) Prof. Dr. Bart Braeckman (Ghent University) Prof. Dr. Didier Ebo (University of Antwerp) Dr. Maarten Aerts (Ghent University) Prof. Dr. Guy Smagghe (Ghent University) Dean: Prof. Dr. Herwig Dejonghe Rector: Prof. Dr. Anne De Paepe The author and the promotor give the permission to use this thesis for consultation and to copy parts of it for personal use. Every other use is subject to the copyright laws, more specifically the source must be extensively specified when using results from this thesis. -
The Genus Hottentotta Birula, 1908, with the Description of a New Subgenus and Species from India (Scorpiones, Buthidae)
©Zoologisches Museum Hamburg, www.zobodat.at Entomol. Mitt. zool. Mus. Hamburg 13(162): 191-195 Hamburg, 1. Oktober 2000 ISSN 0044-5223 The genus Hottentotta Birula, 1908, with the description of a new subgenus and species from India (Scorpiones, Buthidae) W il s o n R . Lo u r e n ç o (With 7 figures) Abstract A new subgenus and species of scorpion,Hottentotta (Deccanobuthus) geffardi sp. n. (Buthidae), are described. The type specimen was collected in Kurduvadi, Deccan Province, India. This specimen had been examined previously by Vachon (pers. comm.), who suggested that it represented a new genus closely allied toButhotus Vachon (= Hottentotta Birula). However, because the precise compositionHottentotta of remains unclear, only a subgenus is proposed at present for this new species. Introduction In the mid-1940s, Vachon started some general studies on the scorpions of North of Africa (see Vachon 1952). One of his main preoccupations was to better define several groups within the family Buthidae, which lead to the division of the genusButhus Leach, 1815 into about 10 different genera. One of the genera proposed by Vachon (1949) was Buthotus, which grouped the majority of the species previously assigned to the subgenus Hottentotta Birula, 1908 (see Vachon & Stockmann 1968). Kraepelin (1891) was the first to distinguish a hottentotta“ group” (species-group) withinButhus. This mainly included species allied Buthusto Hottentotta (Fabricius, 1787). Birula (1908) created the subgenusHottentotta , but Vachon (1949), without explanation, discarded both Hottentotta Birula, 1908 and Dasyscorpio Pallary, 1938 establishing a new name, Buthotus, instead. Hottentotta is, however, a valid senior synonym and was re established by Francke (1985). -
Review on Bioactive Peptides and Pharmacological Activities Of
mac har olo P gy Wang et al., Biochem Pharmacol (Los Angel) 2015, 4:2 : & O y r p t e s DOI: 10.4172/2167-0501.1000166 i n A m c e c h e c s Open Access o i s Biochemistry & Pharmacology: B ISSN: 2167-0501 Review Article Open Access Review on Bioactive Peptides and Pharmacological Activities of Buthus martensii Karsch Jihui Wang*, Hui Zhang, Shijie Gao, Junxiang Wang School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, People’s Republic of China Abstract The Asian scorpion Buthus martensii Karsch has been used in Chinese Traditional Medicine for thousands of years. The scorpion, venom of scorpion and their extracts are effective in treating a variety of nervous system diseases such as epilepsy, apoplexy pains and facial paralysis. Apart from these therapeutic effects, the peptides purified from scorpion also showed antitumor activities, antimicrobial effects and protective effects on vascular. In this review, we reported various pharmacological effects of bioactive peptides purified from scorpion Buthus martensii Karsch so that we can use different effects of these bioactive peptides to treat different diseases and provide reference for the study of scorpion Buthus martensii Karsch in future. Keywords: Buthus martensii Karsch; Bioactive peptides; vascular disease and so on (Table 1). Although the clinical researches Pharmacological activities about pharmacological effects of BmK is less, BmK as a alternative and complementary medicine has been widely used. Introduction Pharmacological Activities of Buthus martensii Karsch Scorpion Buthus martensii Karsch (BmK) is one of the most ancient animals widely distributed in Mongolia, Korea and China. -
The First Data on the Fauna and Geographical Distribution of Medically Important Scorpions in Golestan Province, Northeast of Iran
The First Data on the Fauna and Geographical Distribution of Medically Important Scorpions in Golestan Province, Northeast of Iran Aioub Sozadeh Golestan University of Medical Sciences and Health Services Ehsan Allah Kalteh Golestan University of Medical Sciences and Health Services Shahin Saeedi Golestan University of Medical Sciences and Health Services Mulood Mohammmadi Bavani ( [email protected] ) Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences Research note Keywords: Scorpion, fauna, spatial distribution, Iran, Golestan Posted Date: December 3rd, 2020 DOI: https://doi.org/10.21203/rs.3.rs-117232/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/14 Abstract Objectives: this study was conducted to determine the medically relevant scorpion’s species and produce their geographical distribution in Golestan Province for the rst time, to collect basic information to produce regional antivenom. Because for scorpion treatment a polyvalent antivenom is use in Iran, and some time it failed to treatment, for solve this problem govement decide to produce regional antivenom. Scorpions were captured at day and night time using ruck rolling and Ultra Violet methods during 2019. Then specimens transferred to a 75% alcohol-containing plastic bottle. Finally the specimens under a stereomicroscope using a valid identication key were identied. Distribution maps were introduced using GIS 10.4. Results: A total of 111 scorpion samples were captured from the province, all belonging to the Buthidae family, including Mesobuthus eupeus (97.3%), Orthochirus farzanpayi (0.9%) and Mesobuthus caucasicus (1.8%) species. -
Sequence Analysis of Lysozyme C from the Scorpion Mesobuthus Eupeus Venom Glands Using Semi- Nested RT-PCR
Iranian Red Crescent Medical Journal ORIGINAL ARTICLE Sequence Analysis of Lysozyme C from the Scorpion Mesobuthus Eupeus Venom Glands Using Semi- Nested RT-PCR M Baradaran1*, A Jolodar2, A Jalali3, Sh Navidpour4, F Kafilzadeh5 1Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran; 3Department of Pharmacology and Toxi- cology, School of Pharmacy, Toxicology Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran; 4Veterinary Parasitology Department of Razi Vaccine and Serum Research Institute, Karaj, Iran; 5Azad Islamic University, Jahrom Branch, Jahrom , Iran Abstract Background: Lysozyme is an antimicrobial protein widely distributed among eukaryotes and prokaryotes and take part in protecting microbial infection. Here, we amplified cDNA of MesoLys-C, a c-type lysozyme from the most common scorpion in Khuzestan Province, Southern Iran. Methods: Scorpions of Mesobuthus eupeus were collected from the Khuzestan Province. Using RNXTM solu- tion, the total RNA was extracted from the twenty separated venom glands. cDNA was synthesized with extract- ed total RNA as template and modified oligo(dT) as primer. In order to amplify cDNA encoding a lysozyme C, semi-nested RT-PCR was done with the specific primers. Follow amplification, the fragment was sequenced. Results: Sequence determination of amplified fragment revealed that MesoLys-C cDNA had 438 bp, encoding for 144 aa residues peptide with molecular weight of 16.702 kDa and theoretical pI of 7.54. A putative 22-amino- acids signal peptide was identified. MesoLys-C protein was composed of one domain belonged to c-type lyso- syme/alphalactalbumin. -
Scorpiones: Buthidae: Hottentotta Tamulus) from India
Research Note Haplotype diversity in the medically important red scorpion (Scorpiones: Buthidae: Hottentotta tamulus) from India Vivek Suranse1, Nitin S. Sawant2, D. B. Bastawade3 and Neelesh Dahanukar1,* 1Indian Institute of Science Education and Research (IISER), G1 Block, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India. 2Wildlife Information Liaison Development (WILD) Society, No. 12 Thiruvannamalai Nagar, Saravanampatti - Kalapatti Road, Saravanampatti, Coimbatore 641 035, Tamil Nadu, India. 3Institute of Natural History Education and Research (INHER), C26/9 Ketan Heights, Kothrud, Pune, Maharashtra 411038, India. *For correspondence: [email protected] 1 Abstract The medically important Indian red scorpion, Hottentotta tamulus, is one of the most poisonous scorpions of Indian subcontinent. We studied the haplotype diversity in eight populations of H. tamulus based on mitochondrial cytochrome oxidase subunit I (COI) partial gene sequence. Analysis revealed 22 haplotypes with a haplotype diversity of 0.941 and nucleotide diversity of 0.023. For the first two codon positions both transition and transversion types of substitutions were equally likely and the test for neutrality was not rejected. However, codon substitution pattern indicated that the gene has experienced purifying selection. Model-based clustering method indicated that the eight populations form three groups that correspond to high, moderate and low rainfall areas, indicating that there is biogeographical separation of haplotypes. Populations from three groups formed distinct clades in maximum likelihood analysis and median joining genetic network and were statistically supported by low within group and high among group variation in analysis of molecular variance. We provide the first account of haplotype diversity in Indian red scorpions and their biogeographical separation. -
A Global Accounting of Medically Significant Scorpions
Toxicon 151 (2018) 137–155 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon A global accounting of medically significant scorpions: Epidemiology, major toxins, and comparative resources in harmless counterparts T ∗ Micaiah J. Ward , Schyler A. Ellsworth1, Gunnar S. Nystrom1 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA ARTICLE INFO ABSTRACT Keywords: Scorpions are an ancient and diverse venomous lineage, with over 2200 currently recognized species. Only a Scorpion small fraction of scorpion species are considered harmful to humans, but the often life-threatening symptoms Venom caused by a single sting are significant enough to recognize scorpionism as a global health problem. The con- Scorpionism tinued discovery and classification of new species has led to a steady increase in the number of both harmful and Scorpion envenomation harmless scorpion species. The purpose of this review is to update the global record of medically significant Scorpion distribution scorpion species, assigning each to a recognized sting class based on reported symptoms, and provide the major toxin classes identified in their venoms. We also aim to shed light on the harmless species that, although not a threat to human health, should still be considered medically relevant for their potential in therapeutic devel- opment. Included in our review is discussion of the many contributing factors that may cause error in epide- miological estimations and in the determination of medically significant scorpion species, and we provide suggestions for future scorpion research that will aid in overcoming these errors. 1. Introduction toxins (Possani et al., 1999; de la Vega and Possani, 2004; de la Vega et al., 2010; Quintero-Hernández et al., 2013). -
A New Locality of Mesobuthus Gibbosus (Brullé, 1832) from Montenegro (Scorpiones: Buthidae)
A New Locality of Mesobuthus gibbosus (Brullé, 1832) from Montenegro (Scorpiones: Buthidae) Oskar Wiśniewski & Barbara Olech August 2015 — No. 205 Euscorpius Occasional Publications in Scorpiology EDITOR: Victor Fet, Marshall University, ‘[email protected]’ ASSOCIATE EDITOR: Michael E. Soleglad, ‘[email protected]’ Euscorpius is the first research publication completely devoted to scorpions (Arachnida: Scorpiones). Euscorpius takes advantage of the rapidly evolving medium of quick online publication, at the same time maintaining high research standards for the burgeoning field of scorpion science (scorpiology). Euscorpius is an expedient and viable medium for the publication of serious papers in scorpiology, including (but not limited to): systematics, evolution, ecology, biogeography, and general biology of scorpions. Review papers, descriptions of new taxa, faunistic surveys, lists of museum collections, and book reviews are welcome. Derivatio Nominis The name Euscorpius Thorell, 1876 refers to the most common genus of scorpions in the Mediterranean region and southern Europe (family Euscorpiidae). Euscorpius is located at: http://www.science.marshall.edu/fet/Euscorpius (Marshall University, Huntington, West Virginia 25755-2510, USA) ICZN COMPLIANCE OF ELECTRONIC PUBLICATIONS: Electronic (“e-only”) publications are fully compliant with ICZN (International Code of Zoological Nomenclature) (i.e. for the purposes of new names and new nomenclatural acts) when properly archived and registered. All Euscorpius issues starting from No. 156 (2013) are archived in two electronic archives: Biotaxa, http://biotaxa.org/Euscorpius (ICZN-approved and ZooBank-enabled) Marshall Digital Scholar, http://mds.marshall.edu/euscorpius/. (This website also archives all Euscorpius issues previously published on CD-ROMs.) Between 2000 and 2013, ICZN did not accept online texts as "published work" (Article 9.8). -
On the Genus Hottentotta Birula 1908, with the Description of a New Species from Chad (Scorpiones, Buthidae) 211-218 © Zoologisches Museum Hamburg;
ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Entomologische Mitteilungen aus dem Zoologischen Museum Hamburg Jahr/Year: 2007 Band/Volume: 14 Autor(en)/Author(s): Lourenco Wilson R. Artikel/Article: On the genus Hottentotta Birula 1908, with the description of a new species from Chad (Scorpiones, Buthidae) 211-218 © Zoologisches Museum Hamburg; www.zobodat.at Hamburg, 15. Oktober 2004 Entomol. Mitt. zool. Mus. Hamburg 14 (170): 211-218 ISSN 0044-5223 On the genus Hottentotta Birula 1908, with the description of a new species from Chad (Scorpiones, Buthidae) W il s o n R. Lo u r e n ç o (With 9 figures) Abstract A new species of scorpion, Hottentotta acostai sp. n. (Buthidae) is described. The type material was collected in a steppe formation South of Tibesti Mountains, the North of Chad. The new species is unquestionably associated with Hottentotta minax (L. Koch, 1875) and related species. Its distribution is located where two of the territories defined by Vachon & Stockmann (1968): ‘Eastern sub-equatorial’ and ‘Western sub-equatorial’, overlap. Keywords: Scorpiones, taxonomy, Hottentotta acostai sp. n., Chad, Tibesti, steppe. Introduction About the middle of the 1940s, Vachon (1952) began a series of studies on the scorpions of North of Africa. One of his main preoccupations was to define the various groups within the family Buthidae. This led to the subdivision of what was then the genus Buthus Leach, 1815 into about 10 separate genera. One of the genera proposed by Vachon (1949) was Buthotus. This comprised the majority of species in the old subgenus Hottentotta Birula, 1908 (Vachon & Stockmann 1968). -
It Was Seen Increase in Scorpion Stings, in Kurak and Yarı Kurak Regions
Received: December 7, 2004 J. Venom. Anim. Toxins incl. Trop. Dis. Accepted: May 30, 2005 V.11, n.4, p.479-491, 2005. Published online: October 30, 2005 Original paper - ISSN 1678-9199. Mesobuthus eupeus SCORPIONISM IN SANLIURFA REGION OF TURKEY OZKAN O. (1), KAT I. (2) (1) Refik Saydam Hygiene Center, Poison Research Center, Turkey; (2) Department of Infectious Diseases, Health Center of Sanliurfa, Turkey. ABSTRACT: The epidemiology and clinical findings of scorpion stings in Sanliurfa region of Turkey, from May to September 2003, were evaluated in this study. Mesobuthus eupeus (M. eupeus) plays a role on 25.8% of the scorpionism cases. This study also showed that intoxications caused by M. eupeus in the southeast of Anatolia region were seen in hot months of the summer, especially on July. Females and people above 15 years old were mostly affected and stung on extremities. Intense pain in the affected area was observed in 98.7% cases, hyperemia in 88.8%, swelling in 54.6%, burning in 19.7%, while numbness and itching were seen less frequently. In our study, the six most frequently observed symptoms were local pain, hyperemia, swelling, burning, dry mouth, thirst, sweating, and hypotension. In this study involving 152 M. eupeus toxicity cases, patients showed local and systemic clinical effects but no death was seen. Autonomic system and local effects characterized by severe pain, hyperemia and edema were dominantly seen in toxicity cases. KEY WORDS: Mesobuthus eupeus, Turkey, scorpionism, epidemiology, clinical symptoms. CORRESPONDENCE TO: OZCAN OZKAN, Veterinary Medicine Laboratory, Refik Saydam Hygiene Center, Poison Research Center, 06100 Ankara, Turkey. -
Genetic Analysis and Ecological Niche Modeling Delimit Species Boundary
bioRxiv preprint doi: https://doi.org/10.1101/652024; this version posted April 18, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Genetic analysis and ecological niche modeling delimit species boundary of the 2 Przewalski’s scorpion (Scorpiones: Buthidae) in arid Asian inland 3 1* 1* 1 1 1,2 4 Xue-Shu Zhang , Gao-Ming Liu , Yu Feng , De-Xing Zhang , Cheng-Min Shi 5 1 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, 6 Chinese Academy of Sciences, Beijing 100101, China 7 2 CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese 8 Academy of Sciences, Beijing 100101, China 9 * Contributed equally 10 Corresponding author: Cheng-Min Shi, [email protected] 11 12 Abstract Reliable delimitation of venomous scorpions is not only consequential to toxicological 13 studies but also instructive to conservation and exploration of these important medical resources. In 14 the present study, we delimited species boundary for the the Przewalski’s scorpion from arid 15 northwest China through a combined approach employing phylogenetic analysis, ecological niche 16 modeling and morphological comparison. Our results indicate that the the Przewalski’s scorpion 17 represent an independent taxonomic unit and should be recognized as full species rank, Mesobuthus 18 przewalsii stat. n.. This species and the Chinese scorpion M. martensii represent the eastern 19 members of the M.