Oral and Posters Presentation XXII National Meeting of Virology & VI Mercosur Meeting of Virology

October, 23 – 26, 2011 – Atibaia, São Paulo, Brazil

62 Oral Presentation - Virology mutations would influence in DAA therapy successful in the FMRP-USP; Av. Bandeirantes, 3900 near future. The profile for this kind of resistance mutation Arthropode-borne are widely spread in the in genotypes needs to be more understood. Americas and are responsible for large outbreaks. Mostly of them, belong to family, genus . In HV30 - LABORATORIAL SURVEILLANCE Brazil eleven flavivirus are described, and among them, OF ANIMAL IN PARÁ STATE (DENV-1, 2, 3 and 4) have been related with the largest outbreaks. Therefore, during the 70’s, Rocio THROUGH CNS SAMPLES RECEIVED, virus (ROCV) caused a large outbreak in the Ribeira Valley- BETWEEN JANUARY AND AUGUST 2011. SP with severe cases of neurological manifestation. Also Barbosa, T.F.S.1, Casseb, L.M.N.1, Pereira, A.S.1, Ferro, ROCV activity was found in Bahia state although no CNS M.N.P.1, Begot, A.L.1,2, Brito, R.M.O.3, Lopes, N.R.S.1, impairment was associated with ROCV infection. Saint Louis Vasconcelos, P.F.C.1, Travassos Da Rosa, E.S.1 virus (SLEV) is also responsible for disease with 1. Instituto Evandro Chagas; IEC; Rod. BR 316 Km 7 s/nº neurological manifestations in Brazil and North America. In CEP:67030-000 Levilândia, Ananindeua/Pa 2006, during a dengue outbreak, SLEV was isolated from a 2. Núcleo de Doenças Endêmicas do Estado do Pará; patient having acute febrile disease. The virus has also been SESPA; Rua Presidente Pernambuco, 489 – Batista detected from encephalitis cases in São José do Rio Preto Campos – CEP: 66015-200 – Belém/PA City. The aim of this study was to perform a serologic survey 3. Centro de Controle de Zoonoses; CCZ; Rod. Aug. to ROCV and SLEV in cases of acute febrile illness from Montenegro, Km 11 s/nº - CEP: 66820-000 – Belém/PA Ribeirão Preto City, 2006. Sera were tested by an IgG-ELISA using recombinant peptides of the domain III of E protein Rabies is an infectious disease with severe involvement (rDIII) from ROCV and SLEV. A number of 635 samples of the central nervous system (CNS) which results in were tested, 21 sera presented monotypic IgG antibodies to encephalitis. It is also a zoonotic disease and therefore the ROCV (3.3% positivity) and 15 (2.36%) to SLEV. Further hosts and reservoirs are sylvatic and domestic animals. studies including neutralization test are necessary in order The objective of the study was to detect the to confirm the specificity of antibodies to ROCV and SLEV. (RabV) in biological materials of different animal species Financial Support: FAPESP from several municipalities of Pará State and which were received in the facilities Rabies Laboratory at the Instituto Evandro Chagas during surveillance activities for the HV31 - SPECIFIC IGG-ANTIBODIES TO ROCIO control of rabies carried out by State Healthy Secretariat AND SAINT LOUIS ENCEPHALITIS VIRUS and Municipal Health Secretariats of Pará Sate, between DETECTED IN PATIENTS DURING A DENGUE January and July 2001. It was received and analyzed a total of 521 CNS samples, 488 canines, 24 felines, 8 bovines e OUTBREAK IN RIBEIRÃO PRETO, SP, 2006 1 equine. RabV was searched in all samples by indirect Reis, V.P., Faringnoli, M., Carvalho, A.C., Delsin, D.L., fluorescent assay (IFA) and by intracerebral inoculation into Badra, J.B., Figueiredo, L.T.M. newborn mice. Seven (1.34%) resulted positive to RabV, all Ribeirão Preto School of Medicine, University of São Paulo; of them from canine, and all proceeded from urban area of FMRP-USP; Av. Bandeirantes, 3900 Marabá (8,75%) in the southern of the state, and only two Arthropode-borne viruses are widely spread in the of them were rover dogs. The result obtained suggest an Americas and are responsible for large outbreaks. Mostly intense circulation of RabV in the municipality of Marabá of them, belong to Flaviviridae family, genus Flavivirus. In and indicate a need of an urgent necessity of improvement Brazil eleven flavivirus are described, and among them, of the control measures including a robust campaign of Dengue virus (DENV-1, 2, 3 and 4) have been related with vaccination of domestic animals in the municipality in order the largest outbreaks. Therefore, during the 70’s, Rocio to avoid the spread of rabies in the region and decreasing of virus (ROCV) caused a large outbreak in the Ribeira Valley- risk an outbreak and the expensive costs of post exposition SP with severe cases of neurological manifestation. Also treatment. ROCV activity was found in Bahia state although no CNS Financial support: IEC and CNPq (process 578094/2008-8) impairment was associated with ROCV infection. Saint Louis encephalitis virus (SLEV) is also responsible for disease with HV31 - SPECIFIC IGG-ANTIBODIES TO ROCIO neurological manifestations in Brazil and North America. In 2006, during a dengue outbreak, SLEV was isolated from a AND SAINT LOUIS ENCEPHALITIS VIRUS patient having acute febrile disease. The virus has also been DETECTED IN PATIENTS DURING A DENGUE detected from encephalitis cases in São José do Rio Preto OUTBREAK IN RIBEIRÃO PRETO, SP, 2006 City. The aim of this study was to perform a serologic survey Reis, V.P., Faringnoli, M., Carvalho, A.C., Delsin, D.L., to ROCV and SLEV in cases of acute febrile illness from Badra, J.B., Figueiredo, L.T.M. Ribeirão Preto City, 2006. Sera were tested by an IgG-ELISA Ribeirão Preto School of Medicine, University of São Paulo; using recombinant peptides of the domain III of E protein

October 2011 Volume 16 – Supplement 1 – Oral and Posters Presentation