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Antiprotozoa Activity of Some Essential Oils

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Antiprotozoa Activity of Some Essential Oils Journal of Medicinal Plants Research Vol. 6(15), pp. 2901-2908, 23 April, 2012 Available online at http://www.academicjournals.org/JMPR DOI: 10.5897/JMPR11.1572 ISSN 1996-0875 ©2012 Academic Journals Review Antiprotozoa activity of some essential oils S. PÉREZ G., M. A. RAMOS-LÓPEZ, E. SÁNCHEZ-MIRANDA, M. C. FRESÁN-OROZCO and J. PÉREZ-RAMOS* Universidad Autónoma Metropolitana-Xochimilco, Departamento de Sistemas Biológicos, Calzada del Hueso 1100, Col. Villa Quietud, C.P. 04960, México. Accepted 1 March, 2012 Protozoa cause many diseases such as leishmaniasis, giardiasis, amebiasis, trichomoniasis and Chagas disease. Metronidazole is the most commonly used drug in antiprotozoal therapy, but metronidazole, as well as other antiprotozoal drugs, can have several secondary effects. Essential oils have been successfully used in the treatment of diseases caused by protozoa. This is a review covering such research from the last 10 years. The main essential oils from 42 plant species demonstrate activity against protozoa, and those from the Lamiaceae family were the most studied with 16 species showing antiprotozoal effects. Other plant families containing species that demonstrate therapeutic effects include Verbenaceae with seven species, Asteraceae with four, Piperaceae with three and Annonaceae with two; the others nine families each had one species. Thymol was the main component found in eight species, followed by eugenol and terpinen-4-ol, which were found in four species, and carvacrol and camphor, which were identified in three and two species, respectively. Key words: Essential oil, diseases, Protozoa. INTRODUCTION Protozoa are single-celled organisms found as parasite generally causes dysentery, and invasive ubiquitous, free-living organisms in the environment. As extraintestinal diseases can occur, resulting in abscesses eukaryotes, protozoa have a membrane-bound nucleus in the liver and, in some cases, the involvement of other with well-defined chromosomes. Protozoal diseases, organs. Amebiasis ranks third worldwide in lethal such as amebiasis, giardiasis, trichomoniasis, infection (Walsh, 1988; Petri and Mann, 1993). leishmaniasis and trypanosomiasis, constitute a major G. lamblia has two morphologic forms: Cysts and public health problem in Latin American countries. trophozoites (Bernard et al., 2001). Cysts, in particular, Protozoa are classified as sporozoa (e.g., Plasmodium are responsible for fecal-oral transmission, as they are species) which are intracellular parasites; flagellates viable in cold water for several months (Bingham et al., (e.g., Giardia lamblia, Leishmania infantum, Trichomonas 1979). This protozoon is responsible for intestinal vaginalis and Trypanosoma cruzi) that possess tail-like infection and diarrhea that may lead to nutritional structures for movement; amoeba (e.g., Entamoeba deficiencies, especially in children. Its disease incidence histolytica) that move using temporary cell body was reported to be 4.6 million episodes, with 2.2 million projections termed pseudopods or ciliates, which move deaths due to diarrhea per year; of these, 1.8 million by beating multiple hair-like structures called cilia. deaths were in developing countries (World Health Infections caused by protozoa can occur through Organization (WHO), 2004). The abundance of Giardia in ingestion of cysts, sexual transmission, or through insect people, animals, and environmental sources suggests vectors. that eradication is not an option. The highest prevalence of E. histolytica is in developing Trichomoniasis is caused by T. vaginalis and is the countries. Although amebiasis can be asymptomatic, this most common non-viral, asexually transmitted infection, with approximately 170 million newly acquired infections worldwide per year. It has been linked to an increase in the risk of HIV transmission (Petrin et al., 1998) as well *Corresponding author. E-mail: [email protected]. as adverse pregnancy outcome, low birth weight, Tel/Fax: 52 55 5483 7253. infertility, postoperative infections and cervical neoplasia 2902 J. Med. Plants Res. (Patel et al., 2000; Schwebke and Burgess, 2004). Some 1988). When they enter a human cell, the T. cruzi men who are infected with T. vaginalis may not display become amastigotes and reproduce within the cell. symptoms but are still infectious carriers of this parasite. Chagas disease also can be acquired by humans through The most effective and commonly used drug in the blood transfusions and organ transplantation (Nda et al., treatment of these three protozoans is metronidazole. 2010), congenitally (that is, from a pregnant woman to her However, this compound has unpleasant side effects baby) (Gürtler et al., 2003), and through oral such as a metallic taste, headache, dry mouth, urticaria, contamination (e.g., foodborne parasite transfer) pruritus, and dark-colored urine. In addition, carcinogenic, (Nóbrega et al., 2009). Acute infection can be lethal, and and teratogenic effects have been documented (Upcroft cardiomyopathy develops in 25 to 30% of infected et al., 1999; Upcroft and Upcroft, 2001). These potential persons (PAHO, 2010). The treatment of this disease toxic and mutagenic effects are the main reasons why it has, until recently, utilized nifurtimox and benznidazole is essential to develop alternative antiprotozoal agents (Rassi and Luquetti, 1992). Treatment of T. cruzi is not with high activity, low toxicity, and high efficacy. very effective, and the drugs often produce side effects. The Leishmania life cycle begins when an infected fly Plants and their extracts and essential oils have been bites and injects the protozoa promastigotes into the skin used for many years in the treatment of several diseases, of the new host. These promastigotes are then including parasite infections (Jones, 1996). The essential phagocytosed by macrophages and transform into oils can be extracted from different parts of the plant, amastigotes, which are able to divide. When the obtained by crushing or by distillation. macrophages burst, they release more amastigotes that We performed a literature review of the essential oils are again phagocytosed to continue the process of that have been shown to possess activity against E. infection. histolytica, G. lamblia, T. vaginalis, T. cruzi, and/or Leishmaniasis includes two major diseases: Cutaneous Leishmania sp. leishmaniasis and visceral leishmaniasis. Cutaneous leishmaniasis, the most common form of the disease, produces skin ulcers and is caused by more than 20 ESSENTIAL OILS AGAINST ENTAMOEBA Leishmania species. Visceral leishmaniasis causes a HISTOLYTICA severe systemic disease that is typically fatal when left untreated. This type of leishmaniasis is caused by Thymus vulgaris L. (Lamiaceae) Leishmania chagasi/infantum, which is an emerging The antiamoebic effects of hydroalcoholic and n-hexane opportunistic disease in patients with AIDS (Desjeux, extracts, as well as of the essential oil, of T. vulgaris 2004). against E. histolytica have been tested. The minimal The treatment of leishmaniasis depends on the form of inhibitory concentration (MIC) of this plant’s the disease. Cutaneous leishmaniasis sometimes heals hydroalcoholic extract, hexane extract, and essential oil on its own and may not require treatment, while visceral after 24 h was 4.0, 4.0, and 0.7 mg/ml, respectively leishmaniasis is treated with pentavalent antimonial drugs (Behnia et al., 2008a). The main components of the or amphotericin B that may produce side effects, such as essential oil are thymol, carvacrol, borneol, and linalool weakness, headache, dizziness, cardiotoxicity, etc. (Goodner et al., 2006), and the antiamoebic activity of the Another parasitosis of major importance on the plant appears to be attributable to these compounds. American continent is caused by T. cruzi. Chagas disease is named after the Brazilian physician Carlos Chagas, who discovered the disease in 1909. It is caused Allium sativum L. (Amaryllidaceae) by the T. cruzi protozoa, which is transmitted to animals and people through insect vectors, and is also referred to The antiamoebic properties of the hydroalcoholic and as American trypanosomiasis. hexanic extracts and the essential oil of Iranian A. The T. cruzi life cycle starts in an animal reservoir, sativum (garlic) were studied, with MICs after 24 h of usually wild or domestic mammals, including humans. A 60.0, 4.0, and 0.4 mg/ml, respectively. After 48 h, the reduviid bug serves as vector. Inside of this vector, the MICs for the hexane extract and essential oil were 3 and protozoa enter the epimastigote stage. After 0.3 mg/ml, respectively, whereas metronidazole had a reproduction, the epimastigotes move onto the rectal cell MIC of 2 µg/ml after 24 h and 1.5 µg/ml after 48 h. These wall, where they then transform into infectious results indicate that longer periods of exposure to the tripomastigotes. Then, when the reduviid bug drinks essential oil decreased the number of viable trophozoites blood from a human, it defecates, releasing the (Behnia et al., 2008b). tripomastigotes contained in its feces and allowing the protozoa to enter the human host through the bite wound or by crossing mucus membranes. The molecules and ESSENTIAL OILS AGAINST G. LAMBLIA proteins on the cytoskeleton of the host cell bind to the surface of the parasite and initiate invasion (Ley et al., The antigiardial activity of the phenol-rich essential oils of Perez et al. 2903 Thymbra capitata L. (Lamiaceae), Origanum virens Ocimum basilicum L. (Lamiaceae) Hoffmanns
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