49 89 2 33 ( )

Graphosoma lineatum (L.) (Hem., Scutelleridae) ٭ 5 5 4 3 2 1

٭ : 1 ([email protected] ) 2

3 4

5

89 /8/20 : 88 /8/6 :

. . ( ) lineatum (L.) . . 5/3 5/4 30 30 4 ) . 48 . ( ( ( 14 13/ ) 36 ( 12 32/ ) 24 . . . . ( 7/4 ) . . Graphosoma lineatum :

glucan 1 16 16 ) α-(1,4)-D- 2 . 17(

1- α-amylases 2- Endoamylases ...... : 50

.( 25 24 1723 ) . 1 .( 12 )9 20(. 17 9 ) (2 ) )9.( . . ( 13 ) 10 )6 .( 22 . . Graphosoma lineatum .( 23) (L.) ( 19) Tenebrio (18) 22 10 ) ( ) molitor L. . 75 × 46 × 40 3 . ( 24 ( 3 )2 . ) . ( . .( 24 22) 2.( )1 .( 26) ) ( 4 . . .( 16 )7 1- Glycosyl hydrolases 2- Prokaryotes and Eukaryotes G. lineatum 3- Monomeric enzyme 4- growth regulators (IGRs) 51 89 2 33 ( )

10 . . ) 10 ( . . . 2( )1 ) 28 ±2 )5 10 ( 70 60 5 5 ( 5 . 16:8 . 10 ) 5/0 . ( . . ( s) 5 5 ( )5 . ( 13 ) . . . 1 10 . 2 24 3 30 27 25 20 15 4 4(. ) . 10 ( ) : . . ( ) ) 10 . ( 48 48 36 24 12 6 3 ( )

. 1- Bicinchoninic acid 2- Bovine serum albomin 3- U/mg protein ...... : 52

( ) 10 10 . . . . ( A) . 20 MSTAT-C ) A1 ( ) ( ) A2 ( . ( B) . . ( ) 20 ) B2 B1 . . ( 10 Excel ( s) . . :

[ (1 / ×) 100 ] B2 B1 A2 A1 ) ( .( 13) ( . ( p > 05/0 ) . . . 1 . . 8 10 12 6 12 ( ) 6 ( ) ) . 1 .( 10 ( ) . 10 1- Substrate 53 89 2 33 ( )

7 6

5

4

3

2 ( ) ) (

1

0

Graphosoma 1 .( pH 95/6 37 ) lineatum

14.( 13 ) . .( 21 ) 52/3 55/4 27(. ) . 23/3 65/2 ) 5/3 5/4 ( . . . . Lygus disponsi Linnavuori . . 5/3 . ...... : 54

L. disponsi ( p > 05/0 ) .( 15 13) . . 2 Stomoxys calcitrans (L.) ( 13 ) 4 Locusta migratoria (L.) Tetraneura ulmi (15 13) 1 .( 10 ) . (L.) 30 21 Bombyx mori (L.) . B. mori . . ) 30 13 23 ( 4 4 T. molitor L . ) .( 13 ) ( 2 13 ( 13 ) 5 . . Spodoptera littoralis 32 10 (Boisduval) . 15( 13 ) L. disponsi . ( ) ( 11) 3 23 23 20 )2 . ( 30 27 25 63/2 63/2 ) . 05/2 05/2 13/4 46/3 91/3 ( 11/3 94/4 15/5 2

1- Hori 4- Ken 2- Lumen 5- Ishaaya et al. 3- Fusé et al. 55 89 2 33 ( )

10 9 8

7

6

5

4

3

( ) ) ( 2

1

0

4 15 20 25 27 30

( )

Graphosoma 2 (pH 95/6 37 ) lineatum

20 18 16

14

12

10

8

6

( ) ) ( 4

2

0

0 3 6 12 24 36 48

()

Graphosoma 3 (pH 95/6 37 ) lineatum

20 3 3 . . . . 36 36 24 L. disponsi ( 15 13 ) . . 48 12 ...... : 56

( )8 . 24 24 ) . 48 6 ( . ( 15 ) . 3 . . ( ) . 3 36 2 L. disponsi ( 15 ) 24 11 9 7 5 3 1 ( ) . ) 12 . ( . 3 74/3 72/3 ) 3 ( ) ( 37/3 . 24 ( 51/3 ) 48 )2/2( . ( 13 ) 1 . Chrysomphalus aonidium (L.) . . 24 Aonidiella aurantii (Maskell) . 21/9 16 67/ . 3/0 /0 556

2- Stylet 1- Svirski 57 89 2 33 ( )

64 7/ 63 33/ .( .( 15 13) /1 776 . /2 913 8/3 02/7 . . ( ) L. disponsi . 4 6 12 .( 12 3/ 26 )8/ . ( 19 37/ ) 6 8 12 .( 15 13) ( )2/6 6 12 . 6 12 8 . 6 8 6 12 .

40 35 30 25 20

15

10 ( ) ) (

5

0

Graphosoma 4 (pH 95/6 37 ) lineatum ...... : 58

. . ) ( . . . . . ( p > 05/0 ) 5 . ( ) . . .

6

5

4 3 2 ( ) ) ( 1

0

10 11 12 13 14 15 16 17

Graphosoma 5 ( ( pH 95/6 37 ) lineatum

59 89 2 33 ( )

) G. lineatum ( . . . .

Trissolcus grandis . 1376 . . 1 Graphosoma lineatum (Hym., Scelionidae) . Eurygaster integriceps (Hem., Scutelleridae) . . 110 .

Trissolcus spp. . 1374 . . 2 .Graphosoma lineatum . . 220 .

. . 1380 . . 3 . . 429 . . in vitro . 1382 . . 4 . . 98 .

. 1385 . . . . . . . 5 . Graphosoma lineatum (Het., Scutelleridae) 106. 106. 91 2 16

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25. Titarenko, E., and Chrispeels, M.J. 2000. cDNA Cloning, biochemical characteriz- ation and inhibition by plant inhibitors of the α-amylases of the western corn rootworm , Diabrotica virgifera virgifera . Insect Biochemistry and Molecular Biology, 30:979-990.

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