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Akhilesh Pandey, M.D., Ph.D. Associate Professor Institute Of

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BIOGRAPHICAL SKETCH NAME POSITION TITLE Akhilesh Pandey, M.D., Ph.D. Associate Professor Institute of Genetic Medicine, Biological Chemistry, Oncology and Pathology EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) Armed Forces Medical College, Pune, India M.D. 1988 Medicine University of Michigan, Ann Arbor, MI Ph.D. 1995 Pathology PROFESSIONAL EXPERIENCE Associate Professor 2006-Present McKusick-Nathans Institute of Genetic Medicine, Departments of Biological Chemistry, Oncology and Pathology, Johns Hopkins University, Baltimore, MD Assistant Professor 2002-2006 McKusick-Nathans Institute of Genetic Medicine, Departments of Biological Chemistry, Oncology and Pathology, Johns Hopkins University, Baltimore, MD Instructor 1998-2002 Department of Pathology, Harvard Medical School, Boston, MA Visiting Scientist 1999-2002 Laboratory of Matthias Mann, Center for Experimental Bioinformatics, University of Southern Denmark, Odense, Denmark Postdoctoral Fellow 1996-1999 Laboratory of Harvey Lodish, Whitehead Institute for Biomedical Research, Cambridge, MA Postdoctoral Fellow 1995-1996 Laboratory of Vishva Dixit, University of Michigan, Ann Arbor, MI Residency 1996-1998 Department of Pathology, Brigham and Women’s Hospital, Harvard (Clinical Pathology) Medical School, Boston, MA Internship 1989-1990 Kilpauk Medical College, Madras, India PROFESSIONAL SOCIETIES American Society for Biochemistry and Molecular Biology American Society for Mass Spectrometry American Association for Cancer Research AWARDS AND HONORS First in Forensic Medicine, University of Pune, 1986 Second Place overall in Medical School Class of 1988 Experimental Pathologist-in-training Award, American Society for Investigative Pathology, 1996 Howard Hughes Medical Institute Physician Postdoctoral Fellowship, 1997 (Declined) Howard Temin Award, National Cancer Institute, 1997 Sidney Kimmel Foundation Scholar, 2003 Beckman Young Investigator Award, 2004 Era of Hope Scholar Award, Department of Defense, 2006 GRANT REVIEWER National Institutes of Health, USA: Proteomics Special Emphasis Panel; 2004, Biodata Management and Analysis Panel, 2005; EDRN Biomarker Panel, 2005 National Science Foundation, USA Science Foundation Ireland, Ireland Netherlands Organization for Scientific Research BBVA Foundation, Bioinformatics Grants Program, Spain Michael J. Fox Foundation for Parkinson’s Research EDITORIAL BOARD/REVIEWER: Editorial Board Member: Genome Research, Clinical Proteomics, Journal of Proteome Research Reviewer: Nature, Nature Genetics, Nature Biotechnology, Cell, Molecular Cell, PNAS, Genome Biology, Journal of Biological Chemistry, Molecular and Cellular Proteomics, Proteomics, Journal of Proteome Research, Analytical Chemistry, Trends in Genetics, Bioinformatics, Nucleic Acids Research Peer-Reviewed Scientific Articles: Pandey, A., Tjoelker, L.W. and Thompson, C.B. (1993). Restricted immunoglobulin junctional diversity in neonatal B cells results from developmental selection rather than homology-based V(D)J joining. Journal of Experimental Medicine. 177, 329-337. Pandey, A., Lazar, D.F., Saltiel, A.R. and Dixit, V.M. (1994). Activation of the Eck Receptor Protein Tyrosine Kinase Stimulates Phosphatidylinositol 3-Kinase Activity. Journal of Biological Chemistry. 269, 30154-30157. Shao, H., Lou, L., Pandey, A., Pasquale, E.B. and Dixit, V.M. (1994). cDNA cloning and characterization of a ligand for the Cek5 receptor protein-tyrosine kinase. Journal of Biological Chemistry. 269, 26606-26609. Shao, H., Pandey, A., Seldin, M., O'Shea, K.S. and Dixit, V.M. (1995). Characterization of B61, the ligand for the Eck receptor protein-tyrosine kinase. Journal of Biological Chemistry. 270, 5636-5641. Pandey, A., Duan, H. and Dixit, V.M. (1995). Characterization of a novel Src-like adapter protein that associates with the Eck receptor tyrosine kinase. Journal of Biological Chemistry. 270, 19201-19204. Pandey, A., Shao, H., Marks, R.M., Polverini, P.J. and Dixit, V.M. (1995). Role of B61, the ligand for the Eck receptor tyrosine kinase, in TNF-α induced angiogenesis. Science. 268, 567-569. Pandey, A., Duan, H., Di Fiore, P. P. and Dixit, V.M. (1995). The Ret receptor protein tyrosine kinase associates with the SH2-containing adapter protein Grb10. Journal of Biological Chemistry. 270, 21461-21463. Angrist, M., Wells, D. E., Chakravarti, A. and Pandey, A. (1995). Chromosomal localization of the mouse Src- like adapter protein (SLAP) gene and its putative human homolog SLA. Genomics. 30, 623-625. Pandey, A., Liu, X., Dixon, J.E., Di Fiore, P. P. and Dixit, V.M. (1996). Direct association between the Ret receptor tyrosine kinase and the Src homology 2-containing adapter protein Grb7. Journal of Biological Chemistry. 271, 10607-10610. Roche, S., Alonso, G., Kazlauskas, A., Dixit, V. M., Courtneidge, S. A. and Pandey, A. (1998). Src-like adaptor protein (Slap) is a negative regulator of mitogenesis. Current Biology. 8, 975-978. Pandey, A., Podtelejnikov, A. V., Blagoev, B., Bustelo, X. R., Mann, M. and Lodish, H. F. (2000). Analysis of receptor signaling pathways by mass spectrometry: Identification of Vav-2 as a substrate of the epidermal and platelet-derived growth factor receptors. Proceedings of the National Academy of Sciences. 97, 179-184. Sosinowski T., Pandey, A., Dixit, V. M. and Weiss, A. (2000). Src-like adaptor Protein (SLAP) is a negative regulator of T cell receptor signaling. Journal of Experimental Medicine 191, 463-474. Pandey, A., Ozaki, K., Baumann, H., Levin, S. D., Puel, A., Farr, A. G., Ziegler, S. F., Leonard, W. J. and Lodish, H. F. (2000). Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nature Immunology 1, 59-64. Pandey, A., Fernandez, M. M., Steen, H., Blagoev, B., Nielsen, M. M., Mann, M. and Lodish, H. F. (2000). Identification of a novel immunoreceptor tyrosine-based activation motif (ITAM) - containing molecule, STAM2, by mass spectrometry and its involvement in growth factor and cytokine receptor signaling pathways. Journal of Biological Chemistry. 275, 38633-38639. Kratchmarova, I., Sosinowski, T., Weiss, A., Witter, K., Vincenz, C. and Pandey, A. (2001). Characterization of the promoter region and genomic structure of murine and human Src like adapter protein (SLAP) genes. Gene. 262, 267-273. Peri, S. and Pandey, A. (2001). A reassessment of the translation initiation codon in vertebrates. Trends in Genetics. 17, 685-687. Steen, H., Küster, B., Fernandez, M., Pandey, A. and Mann, M. (2001). Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Analytical Chemistry. 73, 1440-1448. Steen, H., Kuster, B., Fernandez, M. M., Pandey, A.* and Mann, M. (2002). Tyrosine phosphorylation mapping of the EGF receptor signaling pathway. Journal of Biological Chemistry. 277, 1031-1039. *Corresponding author Pandey, A.,* Ibarrola, N., Kratchmarova, I., Fernandez, M. M., Constantinescu, S. N., Ohara, O., Sawasdikosol, S., Lodish, H. F. and Mann, M. (2002). A novel Src homology 2 domain-containing molecule, Src-like adapter protein-2 (SLAP-2), which negatively regulates T cell receptor signaling. Journal of Biological Chemistry. 277, 19131-19138. *Corresponding author Blagoev, B., Nielsen, M. M., Angrist, M., Chakravarti, A. and Pandey, A. (2002). Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr gene. Gene. 284, 161-168. Ong S. E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., Steen, H., Pandey, A.* and Mann, M. (2002). Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Molecular and Cellular Proteomics. 1, 376-386. *Corresponding author Pandey, A.,* Dan, I., Kristiansen, T. Z., Watanabe, N. W., Voldby, J., Kajikawa, E., Khosravi-Far, R., Blagoev, B. and Mann, M. (2002). Cloning and characterisation of PAK5, a novel member of mammalian p21-activated kinase-II subfamily that is predominantly expressed in brain. Oncogene. 21, 3939-3948. *Corresponding author Schiemann, W. P., Blobe, G. C., Kalume, D. E., Pandey, A. and Lodish, H. F. (2002). Context-specific effects of fibulin-5 (DANCE/ EVEC) on cell proliferation, motility, and invasion: Fibulin-5 is induced by TGF-beta and affects protein kinase cascades. Journal of Biological Chemistry. 277, 27367-27377. Dan, I., Watanabe, N. M., Kajikawa, E., Ishida, T., Pandey, A. and Kusumi, A. (2002). Overlapping of MINK and CHRNE gene loci in the course of mammalian evolution. Nucleic Acids Research. 30, 2906-2910. Blagoev, B., Kratchmarova, I., Nielsen, M. M., Fernandez, M. M., Voldby, J., Andersen, J. S., Kristiansen, K. Pandey, A.* and Mann M. (2002). Inhibition of adipocyte differentiation by resistin-like molecule alpha. Biochemical characterization of its oligomeric nature. Journal of Biological Chemistry. 277, 42011-42016. *Corresponding author Blagoev, B., Nielsen, M. M., Angrist, M., Chakravarti, A. and Pandey, A. (2002). Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr gene. Gene. 284, 161-168. Dan, I., Ong, S. E., Watanabe, N. M., Blagoev, B., Nielsen, M. M., Kajikawa, E., Kristiansen, T. Z., Mann, M. and Pandey, A. (2002). Cloning of MASK, a novel member of the mammalian germinal center kinase-III subfamily, with apoptosis-inducing properties.
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  • Lecture Slides

    Lecture Slides

    (J. American Chemical Association, 78, 3458-3459) The Secondary Structure of Complementary RNA E. Peter Geiduschek, John W. Moohr, and Smauel B. Weiss, Proceedings of The National Academy of Sciences, 48, 1078-1086, 1962. R.H. DOI RH, and S. SPIEGELMAN Homology test between the nucleic acid of an RNA virus and the DNA in the host cell. Science 1962 Dec 14 1270-2. MONTAGNIER L, SANDERS FK. REPLICATIVE FORM OF ENCEPHALOMYOCARDITIS VIRUS RIBONUCLEIC ACID. Nature. 1963 Aug 17;199:664-7. (Science 143, 1034-1036, March 6, 1964) WARNER RC, SAMUELS HH, ABBOTT MT, KRAKOW JS. (1963) Ribonucleic acid polymerase of Azotobacter vinelandii, II. Formation of DNA- RNA hybrids with single-stranded DNA as primer. Proc Natl Acad Sci U S A. 49:533-8. Double Stranded RNA as a Specific Biological Effector December 8, 2006 Karolinska Institute, Stockholm, Sweden Viral interference (Interferon) effects in animals M. Hoskins (1935) A protective action of neurotropic against viscerotropic yellow fever virus in Macacus rhesus. American Journal of Tropical Medicine, 15, 675-680 G. Findlay and F. MacCallum (1937) An interference phenomenon in relation to yellow fever and other viruses. J. Path. Bact. 44, 405-424. A. Isaacs and J. Lindenmann (1957) Virus Interference. I. The Interferon Proc. Royal Soc. B 147, 268-273. Proceedings of the National Academy of Sciences, USA, Volume 58, Pages 782-789. 1967 Promoter Make transgenic worms geneX Antisense Transcripts Interference (Development 113:503 [1991]) geneX Promoter Make transgeneic worms geneX SENSE Transcripts Also Interference! (Development 113:503 [1991]) In Vitro Promoter Make RNA in vitro geneX Antisense RNA Inject worm gonad Interference! (Guo and Kemphues, 1995) In Vitro geneX Promoter Make RNA in vitro geneX SENSE RNA Inject worm gonad Also Interference! (Guo and Kemphues, 1995) Craig Mello's RNAi Workshop: 1997 C.