1 First genetic evidence that invasive bullhead (Cottus L. 1758) in Scotland is of English 2 origin and the difficulty of resolving the European Cottus species taxonomy 3 J. McLeish, R. A. Briers, J. A. Dodd, S. Rueckert 4 Edinburgh Napier University, School of Applied Sciences, Edinburgh, UK 5 6 Correspondence 7 Jenny McLeish, Jacobs, 160 Dundee Street, Edinburgh, EH11 1DQ, UK. 8 Email:
[email protected];
[email protected] 9 Telephone: 0131 455 2490 10 11 Funding information 12 This work received funding from the MASTS pooling initiative (The Marine Alliance for 13 Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS 14 is funded by the Scottish Funding Council (grant reference HR09011) and contributing 15 institutions. 16 17 18 19 20 21 22 23 24 1 25 Abstract 26 The European bullhead (Cottus gobio) is widely distributed across Europe and within the UK 27 is native to England and Wales, where it is protected under the Habitats Directive. In 28 Scotland, however, the species is considered invasive and thriving populations are recorded 29 in the Forth and Clyde river catchments, and the Ale Water in the Scottish Borders. The 30 genetic identity of the Scottish populations has not been established. There is also debate 31 about the status of the European bullhead and its validity as single species, a species 32 complex with several unresolved species, or distinct different species in its European 33 distribution range. There is therefore a need to determine the taxonomy and likely source of 34 the novel Scottish populations.