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NPDN Fifth NationalBugwood.org Manoa, at Hawaii of MeetingUniversity Brooks, Fred © AGENDA AT A GLANCE Monday Tuesday Wednesday Thursday Registration opens 7:30 am Registration opens 7:30 am The importance of diagnostics General sessions 8:00–9:30 8:00–10:00 Workshops 8:00–noon Refreshment & networking break Tours Fungal workshop, part 1 Refreshment & networking break 8:00–1:00 Bacterial workshop, part 1 Diagnostic impacts of Meet in the hotel lobby Strategic planning revised taxonomy Box lunch provided Reporting and discussion 10:00–noon 10:30–noon Lunch & poster/exhibit Lunch Lunch & NPDN awards noon-1:00 viewing noon–1:15 noon–1:15 Regional meetings* Techniques, taxonomy and 1:30–3:00 technology *see detailed agenda for more Workshops 1:30–3:00 1:00–5:00 information Fungal workshop, part 2 Refreshment & networking break Refreshment & networking break Bacterial workshop, part 2 Breakout session Validation and verification Discussions and problem solving 3:30–4:30 3:30–5:00 Meeting wrap up Operations Committee meeting* Reception 5:00–7:30 5:30–7:30 *committee members only Exhibitor presentations, networking & poster viewing 8 am 9 am 10 am 11 am noon 1 pm 2 pm 3 pm 4 pm 5 pm 6 pm 7 pm agenda at a glance_2019.indd 1 3/29/2019 12:50:11 PM NPDN Fifth National Meeting AGENDA AT A GLANCE Monday Tuesday Wednesday Thursday Registration opens 7:30 am Registration opens 7:30 am The importance of diagnostics General sessions 8:00–9:30 8:00–10:00 Workshops 8:00–noon Refreshment & networking break Tours Fungal workshop, part 1 Refreshment & networking break 8:00–1:00 Bacterial workshop, part 1 Diagnostic impacts of Meet in the hotel lobby Strategic planning revised taxonomy Box lunch provided Reporting and discussion 10:00–noon 10:30–noon Lunch & poster/exhibit Lunch Lunch & NPDN awards noon-1:00 viewing noon–1:15 noon–1:15 Regional meetings* Techniques, taxonomy and 1:30–3:00 technology *see detailed agenda for more Workshops 1:30–3:00 1:00–5:00 information Fungal workshop, part 2 Refreshment & networking break Refreshment & networking break Bacterial workshop, part 2 Breakout session Validation and verification Discussions and problem solving 3:30–4:30 3:30–5:00 Meeting wrap up Operations Committee meeting* Reception 5:00–7:30 5:30–7:30 *committee members only Exhibitor presentations, networking & poster viewing agenda at a glance_2019.indd 1 3/29/2019 12:50:11 PM DIAGNOSTICS PAST, PRESENT & FUTURE & PRESENT PAST, © Fred Brooks, University of Hawaii at Manoa, Bugwood.org Manoa, at Hawaii of University Brooks, Fred © Executive Director, National Plant Diagnostic Network Diagnostic Plant National Director, Executive Stack Jim meeting! your andenjoy WELCOME planthealthlocallyandnationally. toprotect manycontributions your of NPDNandfor doinsupport allyou for appreciation oursincere Iextendtoyou Committee, behalfoftheNPDNExecutive On Conferences). (Purdue Amanda Shields coordinator andtheconference Goswami(NIFA) Rubella (CERIS), Hill (WPDN),Mike Brenes-Arguedas Tania (NEPDN), Snover-Clift Karen, (GPDN), ChristianWebb (NCPDN), Byrne (SPDN),Jan Chairs, CarrieHarmon Committee Special thankstothe for everyone. experience toensuringanexcellent given effort the tremendous for pages 48–49) (see committees Meeting thetimetothank membersoftheNPDNNational take Please valuetoNPDNdiagnosticians. and providing amongNPDNlabs diversity thegreat for whileaccounting andperformance, documentsprogress improvement, constant facilitates that inaframework NIFA USDA from therequirements addresses that future NPDN’s for avision Plan offers Strategic TheNPDN oftheSP. comments tohelpguidetheimplementation your offer Please Groups. the SPWorking from postersandreports SPthrough onthat andcomment an opportunitytoreview willhave thismeeting,you At planningtoimplementation. nowtransitioningfrom are than tobequick.We importanttogetitright itismore longerthanimagined,butthedrivingsentimenthasbeenthat has taken in2016.TheSPprocess planning(SP)process undertookastrategic we NIFA, ofUSDA theencouragement At YOU! groups—THANK working committees and attitude amongallNPDN isthe identified.That andresolutions which eachissuewasaddressed ofengagementandtheseriousnesswith bythelevel nothelpbutbeimpressed meeting, Icould Diagnosticians IT- themostrecent At oftheNPDNmembership—allyou. dedication andcontinued work ofthehard result adirect ofNPDNare andthesolidreputation criticalrelationships thestrong The manyaccomplishments, pestsandpathogens. emerging,andintroduced recurrent, plantsystemsfrom joint goalofprotecting agencieswiththe regulatory andfederal withstate relationships working strong forged have NPDN.We emulate lookingto nations withseveral andinternationally nationally respected NPDNiswell biosecurity infrastructure. toournational andcontributions toaccomplishments ofwithrespect the NPDNerawithmuchtobeproud years into 17 now We are regions today. five similartoNPDN’s remarkably were activities; thosesixregions andoutreach research coordinate regions to systems.Theyidentifiedsix production ourfood to safeguard U.S. inthe theleadershipofAPSfirst suggestedaplanthealthnetwork since 100years isapproximately It Network. Diagnostic Plant National Meeting ofthe National tothefifth Welcome NPDN fifth national meeting fifthnational NPDN WELCOME Diagnostics: Past, Present & Future Present Past, Diagnostics: 3 8 am 9 am 10 am 11 am noon 1 pm 2 pm 3 pm 4 pm 5 pm 6 pm 7 pm THANK YOU TO OUR SPONSORS! platinum sponsors gold sponsors silver sponsors non-profit sponsors 4 National Plant Diagnostic Network • Fifth National Meeting NPDN fifth national meeting TABLE OF CONTENTS 2 agenda at a glance 3 welcome letter 6 program agenda 10 oral abstracts 17 award recepients 21 poster abstracts 48 meeting committees 50 author index 55 hotel map The 2019 program book was designed and edited by Rachel McCarthy & Dawn Dailey O’Brien, Cornell University. The front cover design was done by Matt Bertone, North Carolina State University. FRONT COVER LEGEND 1. Advanced laboratory, top floor of Agronomy Building, Cornell University (CU) ©Plant Pathology Herbarium 2.Tomato spotted wilt virus (TSWV) infection in Lysimachia procumbens ©Mike Munster, North Carolina State University (NCSU) 3. Gypsy moth caterpillar (Lymantria dispar) ©Matt Bertone, NCSU 4. Hyperspectral image sensing of regions of interest (ROI) for chlorophyll content (REIP) and the xanthophyll cycle (PRI) in non-stress acclimated and stress acclimated Arapidopsis plants ©Behmann et al. (2018) Sensors, 18, 441; doi:10.3390/s18020441 5. Gloeosporium fructigenum circa 1892–1918 ©Atkinson, George Francis, 1854– 1918, CU. Plant Pathology Herbarium, G.F. Atkinson Herbarium 6. Galleries made by emerald ash borer (Agrilus planipennis) in ash (Fraxinus) ©Matt Bertone, NCSU 7. Cedar apple rust (Gymnosporangium juniperi-virginianae) ©Matt Bertone, NCSU 8. Dutch elm disease (Ophiostoma novo-ulmi) fruiting bodies ©Sandra Jensen, CU 9. Flatid planthopper nymph (Flatidae) on wild blackberry (Rubus) ©Matt Bertone, NCSU 10. Powdery mildew on red clover ©Matt Bertone, NCSU 11. Confocal laser scanning microscopy (CLSM) image showing the site of attachments of mandibular muscles trough the larval head cuticle of the fall armyworm (Spodoptera frugiperda) ©Flor Edith Acevedo & István Mikó 12. Illustration of codling moth (Cydia pomonella) life history in apple ©Saunders (1883) Insects Injurious to Fruits 13. Botryosphaeria dothidea canker in maple (Acer) stem ©Matt Bertone, NCSU 14. Whitefly pupa (Trialeurodes ruborum) on blackberry (Rubus) leaf ©Matt Bertone, NCSU 15. Nun moth (Lymantria monacha) and pine-tree lappet (Dendrolimus pini) ©Rachel McCarthy, CU Diagnostics: Past, Present & Future 5 NPDN fifth national meeting PROGRAM AGENDA Monday, April 15 WORKSHOPS 7:30am Registration LOCATION: Grand Hall Foyer 8:00 – 12noon Fungal Workshop Part 1, Megan Romberg, USDA-APHIS-PPQ-PHP-NIS LOCATION: Baltimore & Ohio (B&O) Bacterial Workshop Part 1, Jeff Jones, University of Florida LOCATION: Chesapeake & Ohio (C&O) 12noon – 1:00pm Lunch provided LOCATION: Grand Hall including Northeast and Southeast 1:00pm – 5:00pm Fungal Workshop Part 2, Megan Romberg, USDA-APHIS-PPQ-PHP-NIS LOCATION: Baltimore & Ohio (B&O) Bacterial Workshop Part 2, Sujan Timilsina, University of Florida LOCATION: Milwaukee Tuesday, April 16 7:30am Registration LOCATION: Grand Hall Foyer The importance of diagnostics LOCATION: Illinois Street Ballroom East and West Moderator: Cheryl Smith 8:00am – 8:30am Ray Hammerschmidt, NCPDN Director, Michigan State University Welcome — NPDN: building on past success and looking forward 8:30am – 9:00am Lindsay DuToit, Washington State University From diagnostician to professor and extension plant pathologist: A foundation in diagnostics for developing a research and extension program to address the Land Grant mission 9:00am – 9:30am Steve Koike, Trical Diagnostics A perspective on plant disease diagnostics in California, past-present-future considerations, and the continuing need for agricultural problem-solving in the golden state 9:30am – 10:00am Refreshment and networking break LOCATION: Grand Hall including Northeast and Southeast 6 National Plant Diagnostic Network • Fifth National Meeting Diagnostic impacts of revised taxonomy LOCATION: Illinois Street Ballroom East and West Moderator: Carrie Harmon 10:00am – 12noon Sujan Timilsina, University of Florida Updating diagnostic approach with changing bacterial taxonomy Megan Romberg, USDA-APHIS-PPQ-PHP-NIS Down the rabbit hole: the relationship between fungal taxonomy and diagnostics Judith Brown, University