Recombinant Human CHRNB3 (C-6His)

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Catalog # CI32 Recombinant Human CHRNB3 (C-6His) Derived from Human Cells Recombinant Human Neuronal Acetylcholine Receptor Subunit Beta-3 is produced by our Mammalian expression system and the target gene encoding Ile25-Leu232 is expressed with a 6His tag at the C- terminus. DESCRIPTION Accession Q05901 Known as Neuronal acetylcholine receptor subunit beta-3 Mol Mass 25.3kDa AP Mol Mass 30-40 kDa, reducing conditions. QUALITY CONTROL Purity Greater than 95% as determined by reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. FORMULATION Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. RECONSTITUTION Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. The product is shipped at ambient temperature. SHIPPING Upon receipt, store it immediately at the temperature listed below. Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. STORAGE Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. Neuronal acetylcholine receptor subunit beta-3(CHRNB3) is a cell membrane protein and belongs to the ligand-gated ion channel (TC 1.A.9) family. CHRNB3 seems to be composed of two different type of subunits: alpha and beta. The CHRNB3 are (hetero) pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of CHRNB3 are encoded by separate genes and have BACKGROUND different primary structure. There are several subtypes of neuronal CHRNB3 that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if like muscle alpha-1, they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits. SDS-PAGE.

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Supplementary Data
Supplementary Methods Mutation and microdeletion screening by high resolution melting High-throughput mutation screening of DIS3L2 exons 1-16 and HDAC4 was performed by Lightscanner high resolution melting analysis (Idaho Technology, Salt Lake City, UT). Exons 17-21 of DIS3L2 were not sequenced due to an apparent genomic duplication and consequent inability to uniquely amplify these exons. DNA samples were amplified using LightScanner mastermix under the manufacturer’s guidelines (Idaho Technology). After PCR, samples were heated at 0.1°C/s in the Lightscanner instrument and fluorescence was collected from 60 to 95°C. Melting curves were analyzed using LightScanner software (v2.0, Idaho Technology). Microdeletion screening across DIS3L2 was performed on paired normal- tumor samples using Lightscanner Lunaprobe SNP genotyping. Seven SNPs, ~60 Kb apart (rs2679184, rs12988522, rs4973500, rs3100586, rs3116179, rs923333 and rs2633254) were amplified in separate reactions and analyzed as above. Detailed conditions and primer/probe sequences are available on request. Variant amplicons were sequenced as described below. Direct sequencing was also performed for all exons where a common polymorphism might mask detection of a mutation by Lightscanner. Samples with known LOH were analyzed entirely by direct sequencing, since Lightscanner detects altered melting profiles of DNA heteroduplexes and these cannot exist in hemizygous samples. Sequencing of candidate genes Direct sequencing of exons and flanking consensus splice signals was performed for DIS3L2, GIGYF2, NPPC, HDAC4, TWIST2 and miR-562. PCR amplification was performed using HotStarTaq Mastermix and Q solution (Qiagen, Valencia, CA); all conditions and primers are available on request. PCR products were treated with shrimp alkaline phosphatase and exonuclease-I (New England Biolabs, Ipswich, MA) and sequenced using BigDye terminator chemistry on a 3730xl sequencer (Applied Biosystems, Foster City, CA).
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HHS Public Access Author manuscript Author ManuscriptAuthor Manuscript Author Alcohol Manuscript Author Clin Exp Res. Author Manuscript Author manuscript; available in PMC 2017 May 01. Published in final edited form as: Alcohol Clin Exp Res. 2016 May ; 40(5): 955–968. doi:10.1111/acer.13056. Gene expression changes in glutamate and GABA-A receptors, neuropeptides, ion channels and cholesterol synthesis in the periaqueductal gray following binge-like alcohol drinking by adolescent alcohol-preferring (P) rats Jeanette N. McClinticka,b, William J. McBridec, Richard L. Bellc, Zheng-Ming Dingc, Yunlong Liud, Xiaoling Xueia,b, and Howard J. Edenberga,b,d,* aDepartment of Biochemistry & Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, United States bCenter for Medical Genomics, Indiana University School of Medicine, Indianapolis, IN 46202, United States cInstitute of Psychiatric Research, Department of Psychiatry, Indiana University School of Medicine, Indianapolis, IN 46202, United States dDepartment of Medical & Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, United States Abstract Background—Binge-drinking of alcohol during adolescence is a serious public health concern with long-term consequences, including increased pain, fear and anxiety. The periaqueductal gray (PAG) is involved in processing pain, fear and anxiety. The effects of adolescent binge drinking on gene expression in this region have yet to be studied. Methods—Male adolescent P (alcohol preferring) rats were exposed to repeated binge-drinking (three 1-h sessions/day during the dark-cycle, 5 days/week for 3 weeks starting at 28 days of age; ethanol intakes of 2.5 – 3 g/kg/session). We used RNA sequencing to assess the effects of ethanol intake on gene expression.
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