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BioMed Research International Molecular Biomarkers: Tools of Medicine Guest Editors: Prabir K. Mandal, Shivani Soni, R. Renee Reams, Tiziano Verri, and Sudhish Mishra Molecular Biomarkers: Tools of Medicine BioMed Research International Molecular Biomarkers: Tools of Medicine Guest Editors: Prabir K. Mandal, Shivani Soni, R. Renee Reams, Tiziano Verri, and Sudhish Mishra Copyright © 2013 Hindawi Publishing Corporation. All rights reserved. This is a special issue published in “BioMed Research International.” All articles are open access articles distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Contents Molecular Biomarkers: Tools of Medicine, Prabir K. Mandal, Shivani Soni, R. Renee Reams, Tiziano Verri, Anita Mandal, and Sudhish Mishra Volume 2013, Article ID 595496, 2 pages Defects in Base Excision Repair Sensitize Cells to Manganese in S. cerevisiae, Adrienne P. Stephenson, Tryphon K. Mazu, Jana S. Miles, Miles D. Freeman, R. Renee Reams, and Hernan Flores-Rozas Volume 2013, Article ID 295635, 9 pages Practical Guidance for Implementing Predictive Biomarkers into Early Phase Clinical Studies, Matthew J. Marton and Russell Weiner Volume 2013, Article ID 891391, 9 pages Circulating microRNAs and Kallikreins before and after Radical Prostatectomy: Are They Really Prostate Cancer Markers?, Maria Giulia Egidi, Giovanni Cochetti, Maria Rita Serva, Gabriella Guelfi, Danilo Zampini, Luca Mechelli, and Ettore Mearini Volume 2013, Article ID 241780, 11 pages ANovelDifferential Predict Model Based on Matrix-Assisted Laser Ionization Time-of-Flight Mass Spectrometry and Serum Ferritin for Acute Graft-versus-Host Disease, Chun-yan Zhang, Shu-hong Wang, Wen-rong Huang, Guang-hong Guo, Zhu-hong Zhang, Wen-jun Mou, Li Yu, and Ya-Ping Tian Volume 2013, Article ID 563751, 14 pages The use of Multidimensional Data to Identify the Molecular Biomarker for Pancreatic Ductal Adenocarcinoma, Liwei Zhuang, Yue Qi, Yun Wu, Nannan Liu, and Yili Fu Volume 2013, Article ID 798054, 6 pages Clinical Evaluation and Cost-Effectiveness Analysis of Serum Tumor Markers in Lung Cancer, Rong Wang, Guoqing Wang, Nan Zhang, Xue Li, and Yunde Liu Volume 2013, Article ID 195692, 7 pages Immune Parameters in The Prognosis and Therapy Monitoring of Cutaneous Melanoma Patients: Experience, Role, and Limitations, Monica Neagu, Carolina Constantin, and Sabina Zurac Volume 2013, Article ID 107940, 13 pages Comparative Gene Expression Profiling in Human Cumulus Cells according to Ovarian Gonadotropin Treatments, Said Assou, Delphine Haouzi, Herve´ Dechaud, Anna Gala, Alice Ferrieres,` and Samir Hamamah Volume 2013, Article ID 354582, 13 pages Aptamers: Novel Molecules as Diagnostic Markers in Bacterial and Viral Infections?,Flavia´ M. Zimbres, Attila Tarnok,´ Henning Ulrich, and Carsten Wrenger Volume 2013, Article ID 731516, 7 pages Distribution of ABO Blood Group and Major Cardiovascular Risk Factors with Coronary Heart Disease, Santanu Biswas, Pradip K. Ghoshal, Bhubaneswar Halder, and Nripendranath Mandal Volume 2013, Article ID 782941, 5 pages Immunomodulatory Effect of Continuous Venovenous Hemofiltration during Sepsis: Preliminary Data, Giuseppe Servillo, Maria Vargas, Antonio Pastore, Alfredo Procino, Michele Iannuzzi, Alfredo Capuano, Andrea Memoli, Eleonora Riccio, and Bruno Memoli Volume 2013, Article ID 108951, 6 pages Acetylcholinesterase as a Biomarker in Environmental and Occupational Medicine: New Insights and Future Perspectives, Maria Giulia Lionetto, Roberto Caricato, Antonio Calisi, Maria Elena Giordano, and Trifone Schettino Volume 2013, Article ID 321213, 8 pages Polyisoprenylated Methylated Protein Methyl Esterase Is Both Sensitive to Curcumin and Overexpressed in Colorectal Cancer: Implications for Chemoprevention and Treatment, Felix Amissah, Randolph Duverna, Byron J. Aguilar, Rosemary A. Poku, and Nazarius S. Lamango Volume 2013, Article ID 416534, 13 pages Emerging Therapeutic Biomarkers in Endometrial Cancer, Peixin Dong, Masanori Kaneuchi, Yosuke Konno, Hidemichi Watari, Satoko Sudo, and Noriaki Sakuragi Volume 2013, Article ID 130362, 11 pages Mouse Prostate Epithelial Luminal Cells Lineage Originate in the Basal Layer Where the Primitive Stem/Early Progenitor Cells Reside: Implications for Identifying Prostate Cancer Stem Cells, Jianjun Zhou, Lionel Feigenbaum, Carole Yee, Hongbin Song, and Clayton Yates Volume 2013, Article ID 913179, 8 pages Hindawi Publishing Corporation BioMed Research International Volume 2013, Article ID 595496, 2 pages http://dx.doi.org/10.1155/2013/595496 Editorial Molecular Biomarkers: Tools of Medicine Prabir K. Mandal,1 Shivani Soni,2 R. Renee Reams,3 Tiziano Verri,4 Anita Mandal,1 and Sudhish Mishra5 1 DepartmentofBiology,EdwardWatersCollege,1658KingsRoad,Jacksonville,FL32209,USA 2 Department of Biological Sciences, Alabama State University, Room No. 325, Life Science Building 1627, Hall Street, Montgomery, AL 36104, USA 3 College of Pharmacy & Pharmaceutical Sciences, Florida A&M University, 1520 M L King Boulevard, Tallahassee, FL 32307, USA 4 Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, I-73100 Lecce, Italy 5 Department of Translational Science and Molecular Medicine, MSU College of Human Medicine and Van Andel Institute, 333BostwickAvenueNE,GrandRapids,MI49503,USA Correspondence should be addressed to Prabir K. Mandal; [email protected] Received 11 October 2013; Accepted 11 October 2013 Copyright © 2013 Prabir K. Mandal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Molecular biomarkers are emerging as the key indices for the showed a significant decrease, highlighting the potential management of patients with significant diseases. Motivated usefulness of kallikreins apart from PSA as potential prostate by the systematic effort to define the human genome, the cre- cancer markers. ation of rapid analytic technologies for evaluating nucleic In the paper entitled “A novel differential predict model acidsandproteinshasprovidedthetechnological“boom” based on matrixx-assisted laser ionization time of flight mass for the development of molecular biomarkers. Collaboration spectrometry and serum ferritin for acute graft-versus-host and cooperation between stakeholders involved in biomarker disease,”. C.-Y. Zhang et al. investigated the possibility of development, application, and regulation may be the most prewarningtheriskofanacutegraft-versus-hostdisease expeditiousroutetowardthetranslationoflaboratorydiscov- (aGVHD) before and after allogeneic hematopoietic stem cell ery into patient management. In summary, intensive research transplantation (allo-HSCT) by serum profiling combining has originated multiple factors or biomarkers that are likely to with serum ferritinin. Their joint prewarning model could be helpful in diagnosis, characterization, and therapy selec- predict the risk of aGVHD, especially severe aGVHD before tion of different patients. A thorough understanding of the transplant which provide a reliable method to continuously relevance of each biomarker will be the key to efficiently monitor the condition of patients. diagnose a disease and direct the patients towards the drugs In the paper entitled “The use of multidimensional data more likely to be of benefit based on their particular profile. to identify the molecular biomarker for pancreatic ductal The papers selected for this special issue represent an adenocarcinoma,” L. Zhuang et al. presented that they have excellent panel for addressing the molecular biomarkers as adopted an integrative approach to simultaneously identify the future tools of medicine. This special issue contains thir- biomarker and generate testable hypothesis from multidi- teen papers. mensional omics data. They have found that PER2 expression In the paper entitled “Circulating microRNAs and kallik- washighlyassociatedwiththesurvivaldata,thusrepresent- reins before and after radical prostatectomy: are they really ing a novel biomarker for earlier detection of pancreatic prostate cancer markers?,” M. G. Egidi et al. presented 38 ductal adenocarcinoma (PDAC). patients with prostate cancer. They suggested that two miR- In the paper entitled “Clinical evaluation and cost- NAs (miR-21 and miR-141) could be involved in post surgi- effectiveness analysis of serum tumor markers in lung cancer,” cal inflammatory processes. Postoperative serum kallikreins R. Wang et al. showed that combinations of four tumor 2 BioMed Research International markers (SCCA, NSE, CEA, and CYFRA21-1) improved the and inhibition are a human biological marker of pesticide sensitivity for lung cancer and different combination panels poisoning. Thus, it is used to study the effects of the exposure had their own usefulness. NSE, CEA, and CYFRA21-1 were to organophosphate and carbamate pesticides on NS in occu- the optimal combination panel with highest Youden’s index pational and environmental medicine. This paper reviews (0.64), higher sensitivity (75.76%), and specificity (88.57%), and discusses the recent findings about AChE, including its which can aid in clinical diagnosis of lung cancer. sensitivity to other pollutants and expression of different In the paper entitled “Immune parameters in the prognosis splice variants.
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  • Cinobufagin, a Bufadienolide, Activates ROS-Mediated Pathways to Trigger Human Lung Cancer Cell Apoptosis in Vivo

    Cinobufagin, a Bufadienolide, Activates ROS-Mediated Pathways to Trigger Human Lung Cancer Cell Apoptosis in Vivo

    RSC Advances View Article Online PAPER View Journal | View Issue Cinobufagin, a bufadienolide, activates ROS- mediated pathways to trigger human lung cancer Cite this: RSC Adv.,2017,7,25175 cell apoptosis in vivo† Panli Peng, ab Junhong Lv,c Changqing Cai,b Shaohuan Lin,c Enqing Zhuob and Senming Wang*a Lung cancer, as the most common malignancy worldwide, is one of the most threatening diseases for human beings. Chan Su, an ethanolic extract from skin and parotid venom glands of the Bufo bufo gargarizans Cantor, is widely used as a traditional Chinese medicine in cancer therapy. Bufadienolides are cardiotonic steroids isolated from the skin and parotid venom glands of toad Bufo bufo gargarizans Cantor with excellent anticancer activity. Unfortunately, little information about the in vivo effects and action mechanisms of bufadienolides on human lung cancer cells is available. Therefore, in this study, the anticancer activities of Cinobufagin (CnBu), bufalin (Bu) and arenobufagin (ArBu) were evaluated in Creative Commons Attribution 3.0 Unported Licence. vivo and in vitro and the underlying mechanisms were elucidated. The results showed that CnBu exhibited higher anticancer efficacy than Bu and AuBu against a panel of five lung cancer cells (A549, NCI-H460, H1299, Sk-mes-1 and Calu-3) with IC50 values ranging from 2.3–6.7 mM. Moreover, CnBu showed much higher selectivity between cancer and normal cells, as suggested by its IC50 value towards BEAS-2B human normal bronchial epithelial cells reaching 22.3 mM. CnBu also significantly inhibited the growth of A549 cells in a dose-dependent manner through anti-migration and anti-invasion.
  • ARTICLE Doi: 10.12032/ATR20200603

    ARTICLE Doi: 10.12032/ATR20200603

    ARTICLE doi: 10.12032/ATR20200603 Asian Toxicology Research A network pharmacology approach combined with animal experiment to investigate the blood enriching effect of Gei herba Wen-Bi Mu1, 2#, Can-Can Duan1, 2#, Zhi-Ping Zhong1, 2, Kuan Chen1, 2, Jian-Yong Zhang1, 2* 1School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; 2Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China. #Authors contributed equally to this article. *Corresponding to: Jian-Yong Zhang. Zunyi Medical University, No. 6 Xuefu West Road, Xinpu District, Zunyi 563000, China. Email: [email protected]. Highlights (1) A network pharmacology approach and animal experiments were established to explore the nourishing blood effect of Lanbuzheng (Gei herba). (2) The main active components, targets and pathways of Lanbuzheng (Gei herba) of blood deficiency were predicted by network pharmacology. (3) It’s verified that Lanbuzheng (Gei herba) can treat blood deficiency by improving the peripheral blood routine index and organ index in animal experiment. Submit a manuscript: https://www.tmrjournals.com/atr ATR | August 2020 | vol. 2 | no. 3 | 109 doi: 10.12032/ATR20200603 ARTICLE Abstract Background: To explore active components of Lanbuzheng (Gei herba) and its underlying complex mechanism in treating blood deficiency induced by chemotherapy drug based on network pharmacology and mice experimental validation. Methods: Active components of Lanbuzheng (Gei herba) were screened by Lipinski’s rule of five. Targets acted with active components were predicted by PharmMapper database, and targets whose function associated with blood deficiency were screened by Therapeutic Target Database and UniProt.