DOCSLIB.ORG

Phytochemical Characterization, in Vitro Antibacterial Activity, in Vivo Acute Toxicity Studies of the Seed Oil of Azadirachta Indica (Neem Oil) in Wistar Rats

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Phytochemical Characterization, in Vitro Antibacterial Activity, in Vivo Acute Toxicity Studies of the Seed Oil of Azadirachta Indica (Neem Oil) in Wistar Rats MOJ Toxicology Research Article Open Access Phytochemical characterization, in vitro antibacterial activity, in vivo acute toxicity studies of the seed oil of Azadirachta indica (neem oil) in Wistar rats Abstract Volume 5 Issue 1 - 2019 The aim of this study was to phytochemically characterize, evaluate in vitro the effect Wirsiy Leonel Ngum,1 Gonsu Hortense,2 of neem oil on some pathogenic bacteria and to investigate in vivo the acute oral toxic Ngameni Barthélémy,3 Tembe Estella,1 effects neem. The neem oil extracted from neem seeds was obtained from the University of 1 Maroua Student Centre (commercial production). Extraction was done by the mechanical Fokunang Charles Ntungwen 1 cold pressing method. The composition was analysed via Gas Chromatography/Mass Department of Pharmacotoxicology and Pharmacokinetics, University of Yaounde 1, Cameroon spectrometry (GC/MS). The in vitro antibacterial activity was conducted through Agar 2Department of Microbiology, and infectious Diseases, disc diffusion and broth macro dilution methods. Tested microorganisms included both University of Yaounde 1, Cameroon standard and clinical isolates: Staphylococcus aureus ATCC25923, Escherichia coli 3Department of Pharmacognosy and Pharmaceutical Chemistry, ATCC35218, Enterococcus faecalis ATCC51299, Aerococcusviridans ATCC11563, University of Yaounde 1, Cameroon Klebsiella. pneumoniae, Salmonella typhi, Shigellaspp, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Staphylococcus aureus. The acute oral toxicity test was Correspondence: Charles Fokunang, Department of performed according to the Organization for Economic Co-operation and Development Pharmacotoxicology and Pharmacokinetics, University of (OECD) guidelines 420 in Wistar rats. Fatty acid analysis resulted in the detection of 5 fatty Yaounde 1, Cameroon, Email acids. GC/MS identified 19 bioactive compounds. Inhibition zone diameters varied from 9 (P. mirabilis) to 14 mm (A. viridans ATCC11563 and E. faecalis ATCC51299). S. aureus Received: January 07, 2019 | Published: January 31, 2019 ATCC25923, E. coli ATCC35218, K. pneumoniae, S. typhi, Shigellaspp, and S. aureus were resistant. It was observed that, the ratio of the minimum inhibitory and bactericidal concentrations was equal to 2 (MBC/MIC=2), implying the bactericidal property of the oil. Oral administration of neem oil at a dose of 3mL/100g in rats caused no lethal effects Keywords: Azadirachta indica A Juss, neem oil, antibacterial activity, acute toxicity Introduction As disease causing bacteria continue to exhibit resistance to antibiotic therapy, essential oils represent a potential weapon against emerging Plant-based antimicrobials have immense potential to combat ‘’superbugs’’. In the Northern region of Cameroon, neem oil is used bacterial, fungal, protozoal and viral diseases without any known side to control constipation, rheumatism, skin infections and also as an effects. The use of plants and its products has a long history that began appetite suppressant. with folk medicine and through the years has been incorporated in traditional and allopathic medicine as well as to extend the shelf life of It is in this context that the seed oil of neem from the neem plant, foods, showing inhibition against bacteria, fungi and yeasts.1 Most of one of the world’s versatile plants will be screened for antibacterial their properties are due to essential oils produced by their secondary activity against pathogenic bacteria as a contribution in a new horizon metabolism. Essential oils also known as volatile oils are products of of combating bacterial antibiotic resistance. The objective of study secondary metabolism of aromatic plants.2 Essential oils are derived was to phytochemically characterize, evaluate in vitro the antibacterial from a variety of natural sources including plants or components activity of the essential of Azadirachta indica A. Juss (neem seed oil) of plants such as flowers, leaves, bark, roots, berries, seeds and/or on some pathogenic strains of bacterial and determine in vivo the fruit.3 Essential oils and extracts from several plant species are able acute oral toxicity of this oil. to control microorganisms related to skin, dental caries, and food spoilage, including Gram-negative and Gram-positive bacteria. Materials and methods Following the success of early antibiotic therapies such as penicillin, Plant material leaders in public health during the mid-20th century declared that the end of infectious diseases was approaching. However infectious 1.5L of the essential oil of neem was bought at the Maroua diseases currently are the second leading cause of death worldwide, University Student Center. A follow up on the method of extraction being responsible for approximately 15million deaths each year. for future extractions at the production site indicated the oil was This is because treatment by antibiotics faces major challenges of extracted through the mechanical method by cold pressing. drug resistance. The active principles present in plants appear to be one of the important alternatives when compared to many synthetic Test microorganisms medicines, because of their less or no side effects, cheap, readily Eleven bacterial strains were used. The microorganisms were available, and their better bioavailability.4 Hence, researchers have obtained from the bacteriology laboratory of the Yaoundé University recently paid attention to safer phytomedicines and biologically active Teaching Hospital (CHUY). They included four standard strains and compounds isolated from plant species used in herbal medicines with seven clinical strains. They are: Staphylococcus aureus ATCC25923, acceptable therapeutic index for the development of novel drugs.4,5 Escherichiacoli ATCC35218, Enterococcus faecalis ATCC51299, Submit Manuscript | http://medcraveonline.com MOJ Toxicol. 2019;5(1):31‒38. 31 © 2019 Ngum et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and build upon your work non-commercially. Copyright: Phytochemical characterization, in vitro antibacterial activity, in vivo acute toxicity studies of the seed oil 32 of Azadirachta indica (neem oil) in Wistar rats ©2019 Ngum et al. Aerococcusviridans ATCC11563, Klebsiella pneumoniae, Salmonella Preparation of inocula bacteria typhi, Shigella spp, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Staphylococcus aureus. Inoculum of test organism was prepared by the direct colony suspension method. From a pure bacterial culture not more than Experimental animals 24hours old, 1to 3 well isolated colonies are transferred in to 5ml of saline solution with the help of a platinum loop. The turbidity of the A total of 20 Wistar albino rats of both sexes (10 males and 10 bacterial suspension is adjusted to match the 0.5McFarland standard females), 6-8 weeks old and of average weight 100g were used for corresponding to 1-2 x108cfu/ml and further diluted to contain 106 the study. All the animals were obtained from the animal house of organisms by adding 100uL of saline solution to obtain bacteria the Faculty of Medicine and Biomedical Sciences (FMBS) -UY1.The density suitable for antimicrobial susceptibility testing.7 rats were maintained at room temperature with a 12h light/dark cycle. The rats were randomized into experimental and control groups of Inoculation of agar plates 5 animals each and housed in clean plastic cages (25cm long, 18cm wide and 14cm tall). Animals were given free access to standard diet To inoculate the agar plates, a sterile swab is dipped into the and water. All experimental procedures were performed according bacterial suspension and the agar inoculated by streaking with the to the Organization for Economic Co-operation and Development swab containing the inoculum. This is repeated two times by rotating (OECD) guidelines 420 in Wistar rats and were approved by the at 60° to ensure an even distribution of the inoculum. Faculty of Medicine and Biomedical Sciences Ethical Committee Application of neem oil impregnated disks with an approval number 0226/UY1/FMSB. Using a sterile forceps, an oil impregnated disk is placed on the Phytochemical characterisation surface of the inoculated plate. After the application of oil disks, the This was done in collaboration with an external laboratory- plates are incubated in an inverted position at 37°C for 18hours. Zones Council for Scientific and Industrial Research (CSIR), department of inhibition were observed and measure using a sliding callipers after of science and technology, Pretoria-South Africa. 50ml of neem the 18hours of incubation. seed oil was packaged and sent to the Centre for Science and Determination of minimal inhibitory concentration Industrial Research(CSIR), Bio-analytical laboratory for chemical characterization of Azadirachta indica oil from neem seed. The method (MIC) and minimal bactericidal concentration (MBC) used was gas chromatography coupled with mass spectrometry. The The broth macro dilution method was used. Only bacteria strains essential oil obtained was dried over anhydrous sodium sulphate and which showed susceptibility to our neem oil by the agar disk diffusion kept at 4°C for analysis. method with a diameter of zone of inhibition greater than or equal to 8mm were used to determine the MIC and MBC by the broth macro Analysis of the essential oils dilution method. GC/MS analysis of the essential oil was carried out on an Agilent 6890 equipped with amass spectrometric
Load more

Recommended publications
  • Pharmacology International No
    Pharmacology International No. 76 June 2011 The semi-annual newsletter from the International Union of Basic and Clinical Pharmacology In This Issue IUPHAR and BPS Collaborate on The Guide to Pharmacology: Coming Soon! Pg 1 Online Portal to Launch The Guide to Pharmacology Global Research in Pg 3 Autumn 2011 in Pharmacology Initiative The British Pharmacological Society (BPS) and the International Union of NIDA-IUPHAR Pg 4 Basic and Clinical Pharmacology (IUPHAR) have agreed to collaborate to Early Career Investigator bring core sets of pharmacological data together online, for the first time. Travel Awards 2011 Anniversaries Pg 6 The collaboration centres on the provision of Hong Kong 25th Pg 7 cross-referenced data from the BPS Guide to Malta 5th Pg 10 Receptors and Channels (GRAC), and the NC- IUPHAR online database (IUPHAR-DB) which Meeting Reports features classes of drug targets and their Week of Hospital Pg 13 ligands. A new, single BPS-IUPHAR online Clinical Pharmacology portal will give access to dynamically updated, in Serbia searchable versions of GRAC and IUPHAR-DB, which will be extensively hyperlinked to each Upcoming Events Pg 18 other and to other online resources, including Division of Clinical Pg 21 suppliers’ websites. Pharmacology Symposium at EACPT ECNP Congress Pg 22 It is intended that the creation of two complementary resources, Latin American Pg 23 consistent in content but different in focus, each carrying the Workshop in Pediatric authoritative backing of both IUPHAR and BPS, will be an immensely Pharmacology Research valuable tool to assist research in pharmacology and drug discovery, Pharmacologists of Pg 24 educate the next generation of biomedical and clinical scientists, and Ukraine National Congress provide the general public with accurate information on how drugs work.
    [Show full text]
  • Rare Diseases: Common Issues in Drug Development Guidance for Industry
    Rare Diseases: Common Issues in Drug Development Guidance for Industry DRAFT GUIDANCE This guidance document is being distributed for comment purposes only. Comments and suggestions regarding this draft document should be submitted within 60 days of publication in the Federal Register of the notice announcing the availability of the draft guidance. Submit electronic comments to https://www.regulations.gov. Submit written comments to the Dockets Management Staff (HFA-305), Food and Drug Administration, 5630 Fishers Lane, Rm. 1061, Rockville, MD 20852. All comments should be identified with the docket number listed in the notice of availability that publishes in the Federal Register. For questions regarding this draft document, contact (CDER) Lucas Kempf at 301-796-1140 or (CBER) Office of Communication, Outreach, and Development at 800-835-4709 or 240-402- 8010. U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Biologics Evaluation and Research (CBER) February 2019 Rare Diseases Revision 1 1212399dft.docx 01/30/19 Rare Diseases: Common Issues in Drug Development Guidance for Industry Additional copies are available from: Office of Communications, Division of Drug Information Center for Drug Evaluation and Research Food and Drug Administration 10001 New Hampshire Ave., Hillandale Bldg., 4th Floor Silver Spring, MD 20993-0002 Phone: 855-543-3784 or 301-796-3400; Fax: 301-431-6353; Email: [email protected] https://www.fda.gov/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/default.htm and/or Office of Communication, Outreach, and Development Center for Biologics Evaluation and Research Food and Drug Administration 10903 New Hampshire Ave., Bldg.
    [Show full text]
  • 202497Orig1s000
    CENTER FOR DRUG EVALUATION AND RESEARCH APPLICATION NUMBER: 202497Orig1s000 CROSS DISCIPLINE TEAM LEADER REVIEW 1. Introduction Marqibo is a liposomal formulation of vincristine, which has been developed with the intention to increase the tolerable dose of the active moiety, vincristine, while reducing its dose limiting neurotoxicity. Marqibo’s NDA 202497 is a 505(b)(2) submission, relying on the data from Vincristine Sulfate Injection as presented in the label for this drug or on published literature to address certain nonclinical sections of the label. Vincristine, a vinca alkaloid, is an approved drug for treatment of acute leukemia. The current NDA 202497 submission is seeking accelerated approval for Marqibo for the treatment of adult (age >18 years) patients with Philadelphia chromosome negative (Ph-) acute lymphoblastic leukemia (ALL) in second or greater relapse or whose disease has progressed following two or more treatment lines of anti- leukemia therapy. Marqibo is administered at a dose of 2.25 mg/m2 IV every 7 days as a 60 minute infusion for a 28-day course of treatment. The NDA was based on the results of a phase 2 single arm study, HBS407, supported by a phase 1/2 single arm dose finding study, VSLI-06. Study HBS407 was an international, multicenter, open-label, single-arm trial to evaluate the effect of Marqibo in adult patients with Ph- ALL in second or greater relapse, or Ph- ALL who failed two or greater number of treatment lines of anti-leukemia chemotherapy. The primary efficacy endpoint of Study HBS407 was the proportion of the patients who achieved CR+CRi.
    [Show full text]
  • Human Hepatocytes As a Tool for Studying Toxicity and Drug Metabolism
    292 Current Drug Metabolism, 2003, 4, 292-312 Human Hepatocytes as a Tool for Studying Toxicity and Drug Metabolism M.J. Gómez-Lechón*, M.T. Donato, J.V. Castell and R. Jover Centro de Investigación, Hospital La Fe, Avda Campanar 21, 46009-Valencia, Spain Abstract: Drugs are usually biotransformed into new chemical species that may have either toxic or therapeutic effects. Drug metabolism studies are routinely performed in laboratory animals but, due to metabolic interspecies differences when compared to man, they are not accurate enough to anticipate the metabolic profile of a drug in humans. Human hepatocytes in primary culture provide the closest in vitro model to human liver and the only model that can produce a metabolic profile of a given drug that is very similar to that found in vivo. However their availability is limited due to the restricted access to suitable tissue samples. The scarcity of human liver has led to optimising the cryopreservation of adult hepatocytes for long-term storage and regular supply. Human hepatocytes in primary culture express typical hepatic functions and express drug metabolising enzymes. Moreover, qualitative and quantitative similarities between in vitro and in vivo metabolism of drugs were observed. Different strategies have been envisaged to prolong cell survival and delay the spontaneous decay of the differentiated phenotype during culture. Thus, hepatocytes represent the most appropriate model for the evaluation of integrated drug metabolism, toxicity/metabolism correlations, mechanisms of hepatotoxicity, and the interactions (inhibition and induction) of xenobiotics and drug-metabolising enzymes. However, in view of limitations of primary hepatocytes, efforts are made to develop alternative cellular models (i.e.
    [Show full text]
  • DB-ALM Protocol N° 42 : Liver Slice Hepatotoxicity Screening System Résumé Experimental Description Test Compounds and Result
    © ECVAM DB-ALM: Protocol DB-ALM Protocol n° 42 : Liver Slice Hepatotoxicity Screening System Hepatotoxicity / Metabolism-mediated Toxicity Leakage of lactate dehydrogenase and alanine aminotransferase from rat and mouse liver slices exposed to the test compound is used as a measure of hepatotoxicity. Résumé The assessment of hepatotoxic effects plays an important role in toxicological studies of new drugs and environmental substances. The liver slice system, which has been widely used for the study of drug mechanisms and interactions, is proposed for routine testing of acute hepatotoxicity. Experimental Description Endpoint and Endpoint Measurement: ALANINE AMINOTRANSFERASE (ALT) LEAKAGE LACTATE DEHYDROGENASE (LDH) LEAKAGE Endpoint Value: EC 50 Experimental System(s): Freehand-cut rat liver slices Freehand-cut mouse liver slices Basic Procedure Liver lobes from rats or mice are sliced, incubated for 1 hour in Krebs Ringer Hepes (KRH) medium and then further divided into 100-120mg portions and incubated with 10 minutes oxygen aeration periods for 1 hour. The tissue fragments are exposed to the test compound for 2 hours. Medium is assayed for enzyme activity at regular intervals during the incubation. Finally, the tissue fragments are homogenized, centrifuged and the total enzyme activity is determined in the supernatant. Test Compounds and Results Summary Acetaminophen Phenacetine Amitriptyline HCl Quinidine sulphate Aspirin Theophylline Chlorpromazine HCl Valproic acid Cimetidine Verapamil HCl Furosemide Carbon tetrachloride Imipramine HCl Methyl alcohol Mechlorethamine (nitrogen mustard) Ethyl alcohol Nifedipine Isopropyl alcohol Pentobarbital sodium Cadmium chloride Discussion This assay is a relatively simple, inexpensive and rapid procedure. Various hepatotoxins can be examined at a wide range of concentrations using only a single animal, since one liver can provide a large number of tissue fragments.
    [Show full text]
  • Drug Metabolism Letters, 2019, 13, 53-63
    Send Orders for Reprints to [email protected] 53 Drug Metabolism Letters, 2019, 13, 53-63 RESEARCH ARTICLE ISSN: 1872-3128 eISSN: 1874-0758 Comparison of Rat and Human Pulmonary Metabolism Using Precision-cut Lung Slices (PCLS) Yildiz Yilmaz1,*, Gareth Williams1, Markus Walles1, Nenad Manevski1, Stephan Krähenbühl2 and Gian Camenisch1 1Pharmacokinetic Sciences, Novartis Institutes for Biomedical Research, Basel, Switzerland; 2Clinical Pharmacology and Toxicology, University Hospital, Basel, Switzerland Abstract: Background: Although the liver is the primary organ of drug metabolism, the lungs also contain drug-metabolizing enzymes and may, therefore, contribute to the elimination of drugs. In this investigation, the Precision-cut Lung Slice (PCLS) technique was standardized with the aims of charac- terizing and comparing rat and human pulmonary drug metabolizing activity. Method: Due to the limited availability of human lung tissue, standardization of the PCLS method was performed with rat lung tissue. Pulmonary enzymatic activity was found to vary significantly with rat A R T I C L E H I S T O R Y age and rat strain. The Dynamic Organ Culture (DOC) system was superior to well-plates for tissue in- cubations, while oxygen supply appeared to have a limited impact within the 4h incubation period used Received: August 03, 2018 here. Revised: September 28, 2018 Accepted: October 08, 2018 Results: The metabolism of a range of phase I and phase II probe substrates was assessed in rat and DOI: human lung preparations. Cytochrome P450 (CYP) activity was relatively low in both species, whereas 10.2174/1872312812666181022114622 phase II activity appeared to be more significant. Conclusion: PCLS is a promising tool for the investigation of pulmonary drug metabolism.
    [Show full text]
  • PAGE2011 Poster Abstracts
    PAGE2011 Poster Abstracts Poster: Absorption and physiology-based PK........................................................................ 13 I-10 Christian Bartels Population PK Model for Pooled Data of Different Oral Diclofenac Formulations .................................................................................................................... 13 I-35 Vicente G. Casabo Bioequivalence trials simulation to select the best analyte for acetylsalicylic acid ........................................................................................................... 14 I-62 Kristin Dickschen Pharmacogenomics of Tamoxifen In Female Patients: A PBPK Model-based Investigation Including The Three Main Metabolites ................................... 16 II-20 Ludivine Fronton Monoclonal Antibody Disposition beyond Target Binding: Impact of FcRn on Clearance and Derivation of Mechanistic Compartment Models ..................... 18 II-40 Emilie Hénin Meta-analysis of Magnetic Marker Monitoring data to characterize tablet movement through the gastrointestinal tract ...................................................................... 20 III-09 Donghwan Lee Development of a population model to describe diurnal and chronokinetic variation in cilostazol pharmacokinetics ..................................................... 22 III-26 Eugeniy Metelkin A Systems Pharmacology Model of Anandamide Dynamics After FAAH Inhibitor Administration ........................................................................................ 24 III-35 Christoph
    [Show full text]
  • Cytochrome P450 Isoform-Specific in Vitro Methods to Predict Drug Metabolism and Interactions
    CYTOCHROME P450 ISOFORM- PÄIVI SPECIFIC IN VITRO METHODS TAAVITSAINEN TO PREDICT DRUG Department of Pharmacology and To x i c o l o g y, METABOLISM AND University of Oulu INTERACTIONS OULU 2001 PÄIVI TAAVITSAINEN CYTOCHROME P450 ISOFORM- SPECIFIC IN VITRO METHODS TO PREDICT DRUG METABOLISM AND INTERACTIONS Academic Dissertation to be presented with the assent of the Faculty of Medicine, University of Oulu, for public discussion in the Auditorium of the Department of Pharmacology and Toxicology, on February 24th, 2001, at 12 noon. OULUN YLIOPISTO, OULU 2001 Copyright © 2001 University of Oulu, 2001 Manuscript received: 23 January 2001 Manuscript accepted: 13 February 2001 Communicated by Professor Eva Brittebo Professor Jyrki Taskinen ISBN 951-42-5900-9 (URL: http://herkules.oulu.fi/isbn9514259009/) ALSO AVAILABLE IN PRINTED FORMAT ISBN 951-42-5899-11 ISSN 0355-3221 (URL: http://herkules.oulu.fi/issn03553221/) OULU UNIVERSITY PRESS OULU 2001 Taavitsainen, Päivi, Cytochrome P450 isoform-specific in vitro methods to predict drug metabolism and interactions Department of Pharmacology and Toxicology, University of Oulu, P.O.Box 5000, FIN-90014 University of Oulu, Finland 2001 Oulu, Finland (Manuscript received: 23 January 2001) Abstract Cytochromes P450 (P450, CYP) are a superfamily of enzymes that participate especially in the oxi- dative metabolism of various xenobiotics and endogenous compounds. The major goal of this study was to characterise suitable methods for routine preclinical in vitro testing of new chemical entities (NCE) and to test the methods for the affinity screening of selected drugs. In vitro methods used involve the utilisation of human liver microsomes for studies with P450- selective reference inhibitors, inhibitory antibodies and cDNA-expressed enzymes in cytochrome P450-catalysed activities and for studying the reactions of selegiline and entacapone.
    [Show full text]
  • Toxicology and Applied Pharmacology July 15-16, 2020 Vienna, Austria
    conferenceseries.com 22nd Euro-Global Summit on Toxicology and Applied Pharmacology July 15-16, 2020 Vienna, Austria SCIENTIFIC PROGRAM SCIENTIFIC PROGRAM DAY 1 Wednesday 15th July 08:30-09:00 Registrations 09:00-09:30 Introduction 09:30-09:50 COFFEE BREAK 09:50-11:50 KEYNOTE LECTURES Meeting Hall 01 MEETING HALL 01 MEETING HALL 02 11:50-13:10 Talks On: Medical Toxicology | Clinical Talks On: Computational Toxicology | Toxicology | Food and Nutritional Toxicology Toxicogenomics | Forensic Toxicology Experimental Toxicology Environmental Toxicology Preclinical Drug Development Heavy Metal Toxicity Nano Toxicology Genotoxicity Assesment Preclinical Safety Assessment Stem Cells and Applications Regulatory Toxicology Analysis of Biomolecules 13:10-13:15 GROUP PHOTO 13:15-14:00 LUNCH BREAK MEETING HALL 01 MEETING HALL 02 14:00-16:00 Talks On: Biochemical and Molecular Talks On: Clinical Pharmacology | Pharmacoge- Toxicology | Analytical Toxicology netics | Molecular Pharmacology Biochemical Toxicology Chemical Toxicity Cellular Toxicology Toxic Chemical Management Molecular Toxicology Predictive Toxicology Toxicity Assessment Drug Safety Biomarkers Risk Charecterization Dose Response Assesment 16:00-16:20 COFFEE BREAK MEETING HALL 01 (16:20-17:00) MEETING HALL 01 (17:00-18:00) Young Research Forum Workshop https://europe.toxicologyconferences.com/ SCIENTIFIC PROGRAM DAY 2 Thursday 16th July 09:00-10:30 KEYNOTE LECTURES Meeting Hall 01 10:30-10:50 COFFEE BREAK MEETING HALL 01 MEETING HALL 02 10:50-12:50 Talks On: Immuno Pharmacology | Experimen-
    [Show full text]
  • Overview of Non-Steroidal Anti-Inflammatory Drugs (Nsaids) in Resource Limited Countries
    MOJ Toxicology Case Report Open Access Overview of non-steroidal anti-inflammatory drugs (nsaids) in resource limited countries Abstract Volume 4 Issue 1 - 2018 The non–steroidal anti-inflammatory drugs (NSAIDs) is a drug class that groups together Charles Ntungwen Fokunang,1 Estella Tembe drugs that provide analgesic (pain-killing) and antipyretic (fever-reducing) effects, and, in 1 2 higher doses, anti-inflammatory effects. The analgesic and anti-inflammatory properties Fokunang, Kechia Frederick, Bathelemy 3 3 acts by inhibiting two recognized isoenzymes of prostaglandin G/H synthase also known Ngameni, Bonaventure Ngadjui as cycl-oxygenase (COX), which are COX 1 and COX 2.The pharmacodynamic action 1Department of Pharmacotoxicology and Pharmacokinentics, of these drugs is mostly mediated by inhibition of COX2, while the adverse reactions are University of Yaoundé, Cameroon 2 largely due to COX1 inhibition.. The NSAID selectively inhibiting COX2 were developed Department of Microbiology, University of Yaoundé, Cameroon 3Department of Phytochemistry and Pharmacognosy, University in the 90s to reduce the risk of gastrointestinal toxicity. NSAID use has been found to be of Yaoundé, Cameroon associated with an increased risk of heart failure in several randomized clinical trials and observational studies. Nevertheless, there is still limited information on the risk of heart Correspondence: Charles Fokunang, Department of failure associated with the use of individual NSAIDs (both COX 2 inhibitors and traditional Pharmacotoxicology and Pharmacokinentics, Faculty of Medicine NSAIDs) in clinical practice in resource poor communities, and especially on their dose- and Biomedical Sciences, University of Yaoundé, Cameroon, Tel response associations. The most prominent members of this group of drugs, are aspirin, +237 670902446, Email [email protected] ibuprofen, naproxen, that are all available over the counter in most countries.
    [Show full text]
  • Alpinetin Inhibits Lung Cancer Progression and Elevates Sensitization Drug-Resistant Lung Cancer Cells to Cis-Diammined Dichloridoplatium
    Journal name: Drug Design, Development and Therapy Article Designation: Original Research Year: 2015 Volume: 9 Drug Design, Development and Therapy Dovepress Running head verso: Wu et al Running head recto: Antitumor activity of alpinetin in lung cancer cells open access to scientific and medical research DOI: http://dx.doi.org/10.2147/DDDT.S92702 Open Access Full Text Article ORIGINAL RESEARCH Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium Lin Wu Objective: Alpinetin is a novel flavonoid that has demonstrated potent antitumor activity in Wei Yang previous studies. However, the efficacy and mechanism of alpinetin in treating lung cancer Su-ning Zhang have not been determined. Ji-bin Lu Methods: We evaluated the impact of different doses and durations of alpinetin treatment on the cell proliferation, the apoptosis of lung cancer cells, as well as the drug-resistant lung cancer cells. Department of Thoracic Surgery, Sheng Jing Hospital of China Results: This study showed that the alpinetin inhibited the cell proliferation, enhanced the apop- Medical University, Shenyang, tosis, and inhibited the PI3K/Akt signaling in lung cancer cells. Moreover, alpinetin significantly People’s Republic of China increased the sensitivity of drug-resistant lung cancer cells to the chemotherapeutic effect of cis-diammined dichloridoplatium. Taken together, this study demonstrated that alpinetin signifi- For personal use only. cantly suppressed the development of human lung cancer possibly by influencing mitochondria and the PI3K/Akt signaling pathway and sensitized drug-resistant lung cancer cells. Conclusion: Alpinetin may be used as a potential compound for combinatorial therapy or as a complement to other chemotherapeutic agents when multiple lines of treatments have failed to reduce lung cancer.
    [Show full text]
  • Sillabus for Students of Pharmaceutical Faculty
    Ministerul Sănătăţii al Republicii Moldova IP Universitatea de Stat de Medicină şi Farmacie “Nicolae RED: 02 Testemiţanu” DATE: 21.12.2013 Catedra Farmacologie şi farmacie clinică PA 7.5.1 Pag. 1/7 SILLABUS Approved Approved At the meeting of the Committee At the meeting of the Pharmacology of the Faculty of Pharmacy and Clinical Pharmacy Department Minutes Nr.___ as of ____________ Minutes Nr.___ as of ____________ Dean of the Faculty of Pharmacy, Head of Department, Dr, associate professor _____________ N. Ciobanu Dr.Hab, professor, ___________V. Gonciar SILLABUS FOR STUDENTS OF PHARMACEUTICAL FACULTY Name of the course: PHARMATOXICOLOGY Code of the course: S.09.O.085 Type of course: Compulsory discipline Total number of hours – 68h, Out of which 17h – lectures, 51h – practical works; Number of credits allocated to the course: 3 credits Name of authors that teach the course: Dr, associate professor V. Cazacu CHISINAU, 2014 Ministerul Sănătăţii al Republicii Moldova IP Universitatea de Stat de Medicină şi Farmacie “Nicolae RED: 02 Testemiţanu” DATE: 21.12.2013 Catedra Farmacologie şi farmacie clinică PA 7.5.1 Pag. 2/7 SILLABUS I. The purpose of the subject of Pharmatoxicology: The formation of basic knowledge, assuming the main concepts and risks of the side effects and possible intoxications, etiology, pathogenesis and clinical manifestations of the most common intoxications which may meet a pharmacist in his practice; the determination of the principles and place of the drugs in pharmacotherapy of these intoxications. II. Training objectives for the subject of Pharmacotoxicology: At the level of knowledge and understanding: to learn the groups of toxic medical substances to determine the toxicokinetics of medical substance (absorption, distribution, biotransformation, elimination pathways) to understand the toxicodynamics of drugs (main effects, mechanism of action) to study side effects to appreciate the main indications and contraindications Upon completion of the subject the student will be able to know: Pharmatoxicology content and its tasks.
    [Show full text]