VPS52 Antibody / SACM2L (F54565)

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VPS52 Antibody / SACM2L (F54565) Catalog No. Formulation Size F54565-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml F54565-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml Bulk quote request Availability 1-3 business days Species Reactivity Human Format Purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Antigen affinity purified UniProt Q8N1B4 Localization Cytoplasmic Applications Immunofluorescence : 1:25 Flow cytometry : 1:25 (1x10e6 cells) Immunohistochemistry (FFPE) : 1:25 Western blot : 1:500-1:2000 Limitations This VPS52 antibody is available for research use only. Western blot testing of human MDA-MB-435 cell lysate with VPS52 antibody. Predicted molecular weight ~82 kDa (isoform 1) and 68 kDa (isoform 2). IHC testing of FFPE human colon carcinoma tissue with VPS52 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining. Immunofluorescent staining of human MCF7 cells with VPS52 antibody (green) and DAPI nuclear stain (blue). Flow cytometry testing of human MDA-MB-435 cells with VPS52 antibody; Blue=isotype control, Green= VPS52 antibody. Description This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. Application Notes The stated application concentrations are suggested starting points. Titration of the VPS52 antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen A portion of amino acids 610-637 from the human protein was used as the immunogen for the VPS52 antibody. Storage Aliquot the VPS52 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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Genome-Wide Approach to Identify Risk Factors for Therapy-Related Myeloid Leukemia
Leukemia (2006) 20, 239–246 & 2006 Nature Publishing Group All rights reserved 0887-6924/06 $30.00 www.nature.com/leu ORIGINAL ARTICLE Genome-wide approach to identify risk factors for therapy-related myeloid leukemia A Bogni1, C Cheng2, W Liu2, W Yang1, J Pfeffer1, S Mukatira3, D French1, JR Downing4, C-H Pui4,5,6 and MV Relling1,6 1Department of Pharmaceutical Sciences, The University of Tennessee, Memphis, TN, USA; 2Department of Biostatistics, The University of Tennessee, Memphis, TN, USA; 3Hartwell Center, The University of Tennessee, Memphis, TN, USA; 4Department of Pathology, The University of Tennessee, Memphis, TN, USA; 5Department of Hematology/Oncology St Jude Children’s Research Hospital, The University of Tennessee, Memphis, TN, USA; and 6Colleges of Medicine and Pharmacy, The University of Tennessee, Memphis, TN, USA Using a target gene approach, only a few host genetic risk therapy increases, the importance of identifying host factors for factors for treatment-related myeloid leukemia (t-ML) have been secondary neoplasms increases. defined. Gene expression microarrays allow for a more 4 genome-wide approach to assess possible genetic risk factors Because DNA microarrays interrogate multiple ( 10 000) for t-ML. We assessed gene expression profiles (n ¼ 12 625 genes in one experiment, they allow for a ‘genome-wide’ probe sets) in diagnostic acute lymphoblastic leukemic cells assessment of genes that may predispose to leukemogenesis. from 228 children treated on protocols that included leukemo- DNA microarray analysis of gene expression has been used to genic agents such as etoposide, 13 of whom developed t-ML. identify distinct expression profiles that are characteristic of Expression of 68 probes, corresponding to 63 genes, was different leukemia subtypes.13,14 Studies using this method have significantly related to risk of t-ML.
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A Yeast Phenomic Model for the Influence of Warburg Metabolism on Genetic Buffering of Doxorubicin Sean M
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VPS52 Antibody (F54565)
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