Convulxin a Heterodimeric C-Type Lectin Shown to Activate Mammalian Platelets Product Description

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FT-R8369A Convulxin A heterodimeric C-type lectin shown to activate mammalian platelets Product Description Name : Convulxin from Crotalus durissus terrificus snake venom Catalog Number : R8369A, 50 µg Structure : CAS: [ 37206-04-5] Molecular Weight : ~84kDa. Properties : Purity: >90% Solubility: Soluble in HEPES Appearance: Lyophilized solid Storage: –20°C Introduction Convulxin is a 72-kDa glycoprotein and a member of the heterodimeric C-type lectin family. The compound acts as a potent platelet activator and has been described to induce platelet prothrombinase activity. Data suggests that Convulxin induces the collagen signaling pathway to activate platelets by interacting with the p62/GPV receptor. Additional studies suggest that Convulxin has the ability to enhance the tryosine phosphorylation of the platelet endothelial cell adhesion molecule-1 (CD31), and binds to native GPIb and GPVI. Technical and Scientific Information Reconstitution Reconstitute in 10mM HEPES buffer, pH 7.2. (We recommend adding a carrier protein such as BSA to the reconstitution buffer and any subsequent dilution buffers to prevent absorption on plastic surfaces.) Assay performed in aggregometer: - Incubate 485µl platelets suspension (5x108 platelets/ml) for 1-2 minutes at 37°C until base line is stable. - Add 5µl 200mM CaCl2. - Add 5µl 200mM MgCl2. - Wait until baseline is stable. - Add 5µl collagen solution (0.5mg/ml) for positive control OR add 5µl convulxin (0.1µg/ml in HEPES). - Measure aggregation at 37°C. P.1 FT-R8369A References - Polgár J. et al., Platelet Activation and Signal Transduction by Convulxin, a C-type Lectin from Crotalus durissus terrificus (Tropical Rattlesnake) Venom via the p62/GPVI Collagen Receptor, J. Biol. Chem., 272: 13576 - 13583 (1997) Article - Fidler T. et al., Abstract 15748: Platelet Specific Knockout of Mitochondrial Superoxide Dismutase (Sod2) Impairs Mitochondrial Function and Increases Convulxin-Stimulated Platelet Activation, Circulation, 128: A15748 (2013) Ordering information Catalog size quantities and prices may be found at http://www.interchim.com. Please inquire for higher quantities (availability, shipment conditions). For any information, please ask : Uptima / Interchim; Hotline : +33(0)4 70 03 73 06 Order on-line or Contact your local distributor Disclaimer : Materials from Uptima are sold for research use only, and are not intended for food, drug, household, or cosmetic uses. Uptima is not liable for any damage resulting from handling or contact with this product. P.2.

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