Product Data Sheet IMQ-84600_2015.09-v1 CD11/CD18 (24) Mouse Monoclonal Antibody Catalog Number: IMQ-84600 Size: 0.1 ml (1 mg/ml) Class: Monoclonal Type: Antibody Clone: 24 Host / Isotype: Mouse / IgG1 Immunogen: Fibronectin-purified human monocytes Myeloma / fusion Cells from immunized Balb/c mice were fused with the Sp2/0-Ag.14 myeloma partners: cell line Species Reactivity: Human Specificity: This antibody recognises integrin alpha L,M,X/Beta 2 (CD11/CD18). Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits which mediate cell-cell and cell-extracellular matrix attachments. Integrin beta2 (CD18) associates with integrin alpha L (CD11a) to form the leukocyte function- associated antigen-1 (LFA-1) with integrin alpha M (CD11b) to form Complement Receptor 3 (CR3) and with integrin alpha X (CD11c) to form Complement Receptor 4 (CR4). Each integrin receptor can bind various Intercellular adhesion molecules (ICAMs). Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Purification: Purified on protein A from tissue culture supernatant. Format: Purified IgG supplied in Phosphate buffered saline (PBS) containing 0.09% Sodium azide Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation,RIA, Western Blot Dilutions: Optimal antibody dilution should be determined by titration Storage: Store at 4°C for one month, or in small aliquots at -20°C for longer periods. Avoid repeated freezing and thawing as this may denature the antibody. Do not store antibodies in frost-free freezers. For long-term storage aliquote all or part of the unit in volumes not less than 20 µl. In small aliquots antibodies tend to adsorb to the sides of the vials. Before aliquoting predilution of 1:2 - 1:20 with a solution containing other proteins such as 5% BSA is recommendable. References: Hogg N, et al. An anti-human monocyte/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue. Cell Immunol. 1985. 92(2):247-53. PMID:2581704 Also known as: Integrin alpha (L,M,X)/beta 2 dianova GmbH ● Warburgstrasse 45 ● 20354 Hamburg, Germany Phone: +49 (0)40 45 06 70 ● Email: [email protected]● www.dianova.com Page 1/2 Product Data Sheet IMQ-84600_2015.09-v1 CD11/CD18 (24) Mouse Monoclonal Antibody Safety Information: This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. This reagent contains 0.09% sodium azide (a poisonous and hazardous substance) as preservative. Although this concentration is not regarded as dangerous to health, appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. This product is for In Vitro experimental use only. Not for Therapeutic or Diagnostic use. This product ("Product") is warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts ("Documentation") and to be free from defects in material and workmanship. Unless otherwise expressly authorized in writing, Products are supplied for research use only. No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the original purchaser of the Product ("Buyer"). No other warranties, express or implied, are granted, including without limitation, implied warranties of merchantability, fitness for any particular purpose, or non infringement. Buyer's exclusive remedy for non- conforming Products during the warranty period is limited to replacement of or refund for the non-conforming Product(s). There is no obligation to replace Products as the result of (i) accident, disaster or event of force majeure, (ii) misuse, fault or negligence of or by Buyer, (iii) use of the Products in a manner for which they were not designed, or (iv) improper storage and handling of the Products. For Research Use Only Temperature Limitation Manufacturer Catalog Number Expiry Consult Instructions for Use Lot Number dianova GmbH ● Warburgstrasse 45 ● 20354 Hamburg, Germany Phone: +49 (0)40 45 06 70 ● Email: [email protected]● www.dianova.com Page 2/2 .