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SAN TA C RUZ BI OTEC HNOL OG Y, INC . KCTD8 (N-17): sc-247240 BACKGROUND APPLICATIONS The BTB (broad-complex, tramtrack and bric a brac) domain, also known as KCTD8 (N-17) is recommended for detection of KCTD8 of mouse, rat and the POZ (POxvirus and Zinc finger) domain, is an N-terminal homodimeriza - human origin by Western Blotting (starting dilution 1:200, dilution range tion domain that contains multiple copies of kelch repeats and/or C 2H2-type 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range zinc fingers. Proteins that contain BTB domains are thought to be involved in 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range transcriptional regulation via control of chromatin structure and function. 1:30-1:3000); non cross-reactive with KCTD family members. KCTD8 (potassium channel tetramerisation domain containing 8) is a 473 Suitable for use as control antibody for KCTD8 siRNA (h): sc-89042, KCTD8 amino acid protein that contains one BTB domain, suggesting a possible siRNA (m): sc-146399, KCTD8 shRNA Plasmid (h): sc-89042-SH, KCTD8 role as a transcriptional regulator. KCTD8 is encoded by a gene located on shRNA Plasmid (m): sc-146399-SH, KCTD8 shRNA (h) Lentiviral Particles: human chromosome 4, which encodes nearly 6% of the human genome and sc-89042-V and KCTD8 shRNA (m) Lentiviral Particles: sc-146399-V. has the largest gene deserts (regions of the genome with no protein encod - ing genes) of all of the human chromosomes. Defects in some of the genes Molecular Weight of KCTD8: 52 kDa. located on chromosome 4 are associated with Huntington’s disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. RECOMMENDED SECONDARY REAGENTS To ensure optimal results, the following support (secondary) reagents are REFERENCES recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 1. Bardwell, V.J. and Treisman, R. 1994. The POZ domain: a conserved pro - (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey tein-protein interaction motif. Genes Dev. 8: 1664-1677. anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 2. Zollman, S., et al. 1994. The BTB domain, found primarily in zinc finger sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- proteins, defines an evolutionarily conserved family that includes several rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- developmentally regulated genes in Drosophila. Proc. Natl. Acad. Sci. USA 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) 91: 10717-10721. with UltraCruz™ Mounting Medium: sc-24941. 3. Chen, C.S., et al. 1995. A mapping study of 13 genes on human chromo - some bands 4q11 →q25. Cytogenet. Cell. Genet. 69: 260-265. STORAGE 4. Ahmad, K.F., et al. 1998. Crystal structure of the BTB domain from PLZF. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of Proc. Natl. Acad. Sci. USA 95: 12123-12128. shipment. Non-hazardous. No MSDS required. 5. Zhou, J., et al. 2005. A novel PDIP1-related protein, KCTD10, that inter - RESEARCH USE acts with proliferating cell nuclear antigen and DNA polymerase δ. Biochim. Biophys. Acta 1729: 200-203. For research use only, not for use in diagnostic procedures. 6. Hillier, L.W., et. al. 2005. Generation and annotation of the DNA PROTOCOLS sequences of human chromosomes 2 and 4. Nature 434: 724-731. See our web site at www.scbt.com or our catalog for detailed protocols and 7. Wang, Y., et al. 2009. KCTD10 interacts with proliferating cell nuclear support products. antigen and its down-regulation could inhibit cell proliferation. J. Cell. Biochem. 106: 409-413. CHROMOSOMAL LOCATION Genetic locus: KCTD8 (human) mapping to 4p13; Kctd8 (mouse) mapping to 5 C3.1. SOURCE KCTD8 (N-17) is an affinity purified goat polyclonal antibody raised against a peptide mapping at the N-terminus of KCTD8 of human origin. PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-247240 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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