DOCSLIB.ORG

Characterisation of the Response of Sphingopyxis Granuli Strain TFA to Anaerobiosis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Characterisation of the Response of Sphingopyxis Granuli Strain TFA to Anaerobiosis Abstract TESIS DOCTORAL Characterisation of the response of Sphingopyxis granuli strain TFA to anaerobiosis YOLANDA ELISABET GONZÁLEZ FLORES 17 TESIS DOCTORAL Characterisation of the response of Sphingopyxis granuli strain TFA to anaerobiosis Memoria presentada por Yolanda Elisabet González Flores para optar al grado de Doctora en Biotecnología, Ingeniería y Tecnología Química Sevilla, noviembre de 2019 El director de la tesis La directora de la tesis Dr. Eduardo Santero Santurino Dr. Francisca Reyes Ramírez Catedrático Profesora contratado doctor Dpto. Biología Molecular e Ingeniería Bioquímica Dpto. Biología Molecular e Ingeniería Bioquímica Universidad Pablo de Olavide Universidad Pablo de Olavide Departamento de Biología Molecular e Ingeniería Bioquímica Programa de Doctorado en Biotecnología, Ingeniería y Tecnología Química (RD: 99/2011) FUNDING AND GRANTS First, I want to thank the institutions that have made possible this PhD project held in Centro Andaluz del Biología del Desarrollo (CABD-CSIC-UPO), in the Departamento de Biología Molecular e Ingeniería Bioquímica at Pablo de Olavide University. This PhD project has been funded by the "Ayuda para Investigación Tutorizada" grant (Reference PPI1402) awarded by Pablo de Olavide University and the FPU grant (Reference FPU16/04203) awarded by the Spanish Government (Ministerio de Educación, Cultura y Deporte). During my PhD, I worked for three months in the Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University (The Netherlands), funded by the EMBO short-term fellowship awarded by EMBO organisation. Finally, this PhD project was also funded by a project awarded by the Spanish Government (Ministerio de Economía y Competitividad) in 2014 (Reference BIO2014-57545-R, "Modelos de regulación global y específica en bacterias degradadoras de contaminantes ambientales"), whose PI was Dr. Eduardo Santero Santurino. AKNOWLEDGMENTS/AGRADECIMIENTOS First of all, I want to apologise, as most of my aknowledments are going to be in Spanish because, as it is my mother language, I think this way I can express better what I have felt and I still feel about this 5-years adventure. Como creo que toda buena historia debe contarse desde el principio, me gustaría empezar por agradecer a las personas y eventos de mi vida que me hicieron decidirme por este camino y no otro durante la carrera, un periodo de mi vida donde estaba siendo bombardeada con tantas cosas que me parecían súper interesantes, que no hubiera sabido donde iba a terminar. Pero en segundo de carrera empecé a ver la luz, cuando comencé a enamorarme de la Microbiología en las clases sobre genética bacteriana de Paqui. ¿Quién me iba a decir que luego iba a ser mi directora de tesis? ¡Qué cosas tiene la vida! Sin embargo, aunque esta fue la semillita de mi decisión, no fue lo único que me motivó a seguir este camino. En tercero de carrera, hubo dos grandes acontecimientos que me harían decantarme definitivamente por este campo. El primero fue participar con compañeros y profesores de esta universidad en el Concurso Internacional de Biología Sintética Igem, que me hizo experimentar por primera vez como era realmente trabajar en un proyecto de investigación en microbiología. Quiero agradecer a los profesores Fernan, Victor, Rafa, Antonio y Luis por confiar en mí para este proyecto y a todos mis compañeros del Igem, por poder compartir con ellos esta experiencia tan enriquecedora. El segundo evento que confirmó mi decisión fueron los 2 meses que pude trabajar en el laboratorio de la doctora Matxalen Llosa Blas en la Universidad de Cantabria, gracias a la beca JAE intro. A Matxalen no solo tengo que agradecerle a nivel profesional, sino también a nivel personal, el trato que recibí allí, donde me hizo sentir muy acogida e integrada. Guardo preciosos recuerdos de ella y de las chicas del laboratorio, Anabel, Coral y Delfina. Así que quiero empezar agradeciendo a todas estas personas que, puede que sin saberlo, me hicieron tomar la decisión de realizar mi tesis doctoral en este precioso campo que es la Microbiología. Pero no basta esta determinación para empezar una tesis. Esto no podría haber sido posible sin la confianza que depositaron en mi mis directores de tesis, Eduardo y Paqui, aquel día hace ya parece que tantos años en los que les pedí que me aceptaran como alumna interna porque quería hacer la tesis en su laboratorio. Gracias a su confianza, estoy aquí escribiendo esto. Les quiero agradecer además haberme apoyado y guiado durante estos 5 años, por su dedicación y esfuerzo, a pesar de tener muchísimo más trabajo que hacer. Hasta el final han estado dándolo todo con las correcciones, para que mi tesis pudiera estar lista a tiempo, Paqui incluso teniéndose que preparar su oposición. Muchísimas gracias a los dos de corazón. Durante estos años de master y tesis he conocido a mucha gente maravillosa en el laboratorio y en el CABD en general. Quisiera agradecer a todos los "grandes", Eva, Inés, Amando, Carlos, Fernan, Aroa, Belén, Laura Tomás, Guada y Nuria, y también a los "pequeños", Rubén, Rocío, Eli, Magali, Inma, Elena, Laura Terrón y Tony. Voy a echar mucho de menos nuestras conversaciones en los sofás después de comer, descubriendo los horóscopos de todas las culturas habidas y por haber, contándonos los cotilleos del centro o organizando las futuras clases de Yoga en el CABD. También echaré de menos nuestras cervezas en el Santa Clara celebrando papers, becas y demás; gracias por acordaros siempre de pedir al menos una carbonara para mí. Con Eva he compartido la afición por la danza, además de por la ciencia; con Carlos y Laura, por otro lado, he compartido muchos gustos frikis (con Carlos incluso un diseño de camiseta). Con Amando recuerdo alguna conversación filosófica sobre la felicidad después de una comida de Navidad y lo feliz que se puso cuando descubrió que me gustaba la cerveza. Fernan por tu apoyo durante toda la tesis, diciéndome que siempre te encantaban mis charlas, no sabes lo que me animaba eso. De Belén, recuerdo con cariño aquellas tardes-noche de curvas de crecimiento interminables en las que solo quedábamos nosotras dos en el laboratorio y antes de irse me preguntaba con cara de preocupación si me quedaba mucho. Y qué decir de Guada, sin ella nuestro laboratorio no podría funcionar. Muchas gracias por estar siempre dispuesta a ayudarnos, a explicarnos todo con paciencia infinita, a encargarte de tantísimas cosas. Creo que no exagero al decir que gran parte de lo que sé hacer en el laboratorio lo aprendí de ella. En cuanto a los "pequeños", en primer lugar agradecer a mi compañero de laboratorio durante todos estos años, Rubén. Lo que nos hemos reído jugando con el hielo seco, o bromeando. Responsable, sin duda, de la mejor banda sonora que haya tenido un laboratorio. Y muchísimas gracias por ayudarme con esas cuantitativas y con los temas bioinformáticos. A Rocío la he echado mucho de menos estos meses, sobretodo cuando se acercan los carnavales. Muchas gracias por todo lo que me has enseñado, no solo de cuantitativas (que también), sino de chirigotas. Disfrutaba muchísimo con nuestras conversaciones carnavaleras ("Se fue Manuel..."). A Inma también la estoy echando mucho de menos, sus conversaciones, sus ataques de risa, Villarriba y Villabajo... Muchas gracias a Magaly por alegrarnos las post-Navidades con esos riquísimos dulces y chocolates que nos traía de su tierra. A Elena también agradecerle todas sus enseñanzas y consejos, además de muchas conversaciones divertidas. De todos vosotros me llevo muchos recuerdos preciosos. También una mención especial a todos los alumnos internos que han pasado por nuestros laboratorios y que recuerdo con mucho cariño, Marina, Jorge, Cristian, Isa, Manu... y muchísimos más, que me marcaron de alguna manera. Fuera de Micro, quería agradecer a los genéticos y gusaneros, con los que también he compartido muy buenos momentos y almuerzos muy divertidos. A mi amiga Marina de Biología celular, que me ha apoyado durante toda la tesis y me ayudó muchísimo con los temas de papeleo de la beca de estancia... ¡Qué habría hecho sin ella! I also want to thank all the people of the Campy lab, that I met during my three months visit in Utrecht. Thanks to Jos van Putten for accepting me in his lab. Thanks to Marc Wösten for guiding my through my experiments there, always attentive, following all my advances and helping me with a wide smile. He and the rest of the lab members made me feel home, including me in all the activities, even the the birthday parties and the Lab Outje. They even made me an goodbye party and a present! Special thanks to Linda, for helping me with the stainings and optical microscopy, being almost more excited than me to see the results. And thanks to Melanie for helping me get those great EM pictures that I'm using for my PhD. I also miss all the other lab members: Nancy, Karin, Alexia, Ana, Danique, Caren, Yvette, Jiannan, Xuefeng, Guus, Xinyue, Carlos, Yaro, Roel, Edwin, Marcel and Yanyi. You are all fantastic!! Y por último, quería agradecer a los que habeís sido mi mayor apoyo durante estos años de tesis, mi familia. Gracias a mis padres, Yolanda y Sergio, y mi tía Eva, por estar siempre ahí, por animarme cuando lo pasaba mal, aguantar mis estreses y mis agobios. A mi novio, Dani, que creo que eres el que más ha tenido que aguantarme durante todos estos años, cuando me daban bajadas de ánimo. ¡Incluso te viniste conmigo a Holanda durante los tres meses de estancia para ayudarme en todo lo que pudieras!. Has demostrado ser, incluso literalmente, mi mejor compañero de viaje. Sé que sin todos vosotros no podría haber llegado hasta aquí. No sé cuantas veces me habréis escuchado decir "Voy a dejar el doctorado", aunque sabíais que lo decía con la misma convicción del "Voy a dejar la carrera".
Load more

Recommended publications
  • Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas Sp
    microorganisms Article Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5 Karel Kopejtka 1 , Yonghui Zeng 1,2, David Kaftan 1,3 , Vadim Selyanin 1, Zdenko Gardian 3,4 , Jürgen Tomasch 5,† , Ruben Sommaruga 6 and Michal Koblížek 1,* 1 Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 379 81 Tˇreboˇn,Czech Republic; [email protected] (K.K.); [email protected] (Y.Z.); [email protected] (D.K.); [email protected] (V.S.) 2 Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark 3 Faculty of Science, University of South Bohemia, 370 05 Ceskˇ é Budˇejovice,Czech Republic; [email protected] 4 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 370 05 Ceskˇ é Budˇejovice,Czech Republic 5 Research Group Microbial Communication, Technical University of Braunschweig, 38106 Braunschweig, Germany; [email protected] 6 Laboratory of Aquatic Photobiology and Plankton Ecology, Department of Ecology, University of Innsbruck, 6020 Innsbruck, Austria; [email protected] * Correspondence: [email protected] † Present Address: Department of Molecular Bacteriology, Helmholtz-Centre for Infection Research, 38106 Braunschweig, Germany. Abstract: An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 Citation: Kopejtka, K.; Zeng, Y.; (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Ty- Kaftan, D.; Selyanin, V.; Gardian, Z.; rolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic Tomasch, J.; Sommaruga, R.; Koblížek, analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas M. Characterization of the Aerobic and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Anoxygenic Phototrophic Bacterium Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%).
    [Show full text]
  • Succession and Persistence of Microbial Communities and Antimicrobial Resistance Genes Associated with International Space Stati
    Singh et al. Microbiome (2018) 6:204 https://doi.org/10.1186/s40168-018-0585-2 RESEARCH Open Access Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces Nitin Kumar Singh1, Jason M. Wood1, Fathi Karouia2,3 and Kasthuri Venkateswaran1* Abstract Background: The International Space Station (ISS) is an ideal test bed for studying the effects of microbial persistence and succession on a closed system during long space flight. Culture-based analyses, targeted gene-based amplicon sequencing (bacteriome, mycobiome, and resistome), and shotgun metagenomics approaches have previously been performed on ISS environmental sample sets using whole genome amplification (WGA). However, this is the first study reporting on the metagenomes sampled from ISS environmental surfaces without the use of WGA. Metagenome sequences generated from eight defined ISS environmental locations in three consecutive flights were analyzed to assess the succession and persistence of microbial communities, their antimicrobial resistance (AMR) profiles, and virulence properties. Metagenomic sequences were produced from the samples treated with propidium monoazide (PMA) to measure intact microorganisms. Results: The intact microbial communities detected in Flight 1 and Flight 2 samples were significantly more similar to each other than to Flight 3 samples. Among 318 microbial species detected, 46 species constituting 18 genera were common in all flight samples. Risk group or biosafety level 2 microorganisms that persisted among all three flights were Acinetobacter baumannii, Haemophilus influenzae, Klebsiella pneumoniae, Salmonella enterica, Shigella sonnei, Staphylococcus aureus, Yersinia frederiksenii,andAspergillus lentulus.EventhoughRhodotorula and Pantoea dominated the ISS microbiome, Pantoea exhibited succession and persistence. K. pneumoniae persisted in one location (US Node 1) of all three flights and might have spread to six out of the eight locations sampled on Flight 3.
    [Show full text]
  • Pneumoniae CG43
    YjcC, a c-di-GMP Phosphodiesterase Protein, Regulates the Oxidative Stress Response and Virulence of Klebsiella pneumoniae CG43 Ching-Jou Huang1, Zhe-Chong Wang2, Hsi-Yuan Huang3, Hsien-Da Huang2,3, Hwei-Ling Peng1,2* 1 Institute of Molecular Medicine and Biological Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China, 2 Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China, 3 Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China Abstract This study shows that the expression of yjcC, an in vivo expression (IVE) gene, and the stress response regulatory genes soxR, soxS, and rpoS are paraquat inducible in Klebsiella pneumoniae CG43. The deletion of rpoS or soxRS decreased yjcC expression, implying an RpoS- or SoxRS-dependent control. After paraquat or H2O2 treatment, the deletion of yjcC reduced bacterial survival. These effects could be complemented by introducing the DyjcC mutant with the YjcC-expression plasmid pJR1. The recombinant protein containing only the YjcC-EAL domain exhibited phosphodiesterase (PDE) activity; overexpression of yjcC has lower levels of cyclic di-GMP. The yjcC deletion mutant also exhibited increased reactive oxygen species (ROS) formation, oxidation damage, and oxidative stress scavenging activity. In addition, the yjcC deletion reduced capsular polysaccharide production in the bacteria, but increased the LD50 in mice, biofilm formation, and type 3 fimbriae major pilin MrkA production. Finally, a comparative transcriptome analysis showed 34 upregulated and 29 downregulated genes with the increased production of YjcC. The activated gene products include glutaredoxin I, thioredoxin, heat shock proteins, chaperone, and MrkHI, and proteins for energy metabolism (transporters, cell surface structure, and transcriptional regulation).
    [Show full text]
  • Download Via Github on RNA-Seq and Chip-Seq Data Analysis and the University of And/Or Bioconductor [33–35, 38–40]
    Kumka and Bauer BMC Genomics (2015) 16:895 DOI 10.1186/s12864-015-2162-4 RESEARCH ARTICLE Open Access Analysis of the FnrL regulon in Rhodobacter capsulatus reveals limited regulon overlap with orthologues from Rhodobacter sphaeroides and Escherichia coli Joseph E. Kumka and Carl E. Bauer* Abstract Background: FNR homologues constitute an important class of transcription factors that control a wide range of anaerobic physiological functions in a number of bacterial species. Since FNR homologues are some of the most pervasive transcription factors, an understanding of their involvement in regulating anaerobic gene expression in different species sheds light on evolutionary similarity and differences. To address this question, we used a combination of high throughput RNA-Seq and ChIP-Seq analysis to define the extent of the FnrL regulon in Rhodobacter capsulatus and related our results to that of FnrL in Rhodobacter sphaeroides and FNR in Escherichia coli. Results: Our RNA-seq results show that FnrL affects the expression of 807 genes, which accounts for over 20 % of the Rba. capsulatus genome. ChIP-seq results indicate that 42 of these genes are directly regulated by FnrL. Importantly, this includes genes involved in the synthesis of the anoxygenic photosystem. Similarly, FnrL in Rba. sphaeroides affects 24 % of its genome, however, only 171 genes are differentially expressed in common between two Rhodobacter species, suggesting significant divergence in regulation. Conclusions: We show that FnrL in Rba. capsulatus activates photosynthesis while in Rba. sphaeroides FnrL regulation reported to involve repression of the photosystem. This analysis highlights important differences in transcriptional control of photosynthetic events and other metabolic processes controlled by FnrL orthologues in closely related Rhodobacter species.
    [Show full text]
  • The Escherichia Coli Narl Receiver Domain Regulates Transcription
    Katsir et al. BMC Microbiology (2015) 15:174 DOI 10.1186/s12866-015-0502-9 RESEARCH ARTICLE Open Access The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions Galit Katsir1,3,4, Michael Jarvis2,5, Martin Phillips1, Zhongcai Ma2,6 and Robert P. Gunsalus2,3* Abstract Background: The Escherichia coli response regulator NarL controls transcription of genes involved in nitrate respiration during anaerobiosis. NarL consists of two domains joined by a linker that wraps around the interdomain interface. Phosphorylation of the NarL N-terminal receiver domain (RD) releases the, otherwise sequestered, C-terminal output domain (OD) that subsequently binds specific DNA promoter sites to repress or activate gene expression. The aim of this study is to investigate the extent to which the NarL OD and RD function independently to regulate transcription, and the affect of the linker on OD function. Results: NarL OD constructs containing different linker segments were examined for their ability to repress frdA-lacZ or activate narG-lacZ reporter fusion genes. These in vivo expression assays revealed that the NarL OD, in the absence or presence of linker helix α6, constitutively repressed frdA-lacZ expression regardless of nitrate availability. However, the presence of the linker loop α5-α6reversedthisrepressionandalsoshowedimpairedDNAbindingin vitro.The OD alone could not activate narG-lacZ expression; this activity required the presence of the NarL RD. A footprint assay demonstrated that the NarL OD only partially bound recognition sites at the narG promoter, and the binding affinity was increased by the presence of the phosphorylated RD. Analytical ultracentrifugation used to examine domain oligomerization showed that the NarL RD forms dimers in solution while the OD is monomeric.
    [Show full text]
  • Pleuropneumoniae Gene Expression
    Global Effects of Catecholamines on Actinobacillus pleuropneumoniae Gene Expression Lu Li, Zhuofei Xu, Yang Zhou, Lili Sun, Ziduo Liu, Huanchun Chen, Rui Zhou* Division of Animal Infectious Diseases in State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Abstract Bacteria can use mammalian hormones to modulate pathogenic processes that play essential roles in disease development. Actinobacillus pleuropneumoniae is an important porcine respiratory pathogen causing great economic losses in the pig industry globally. Stress is known to contribute to the outcome of A. pleuropneumoniae infection. To test whether A. pleuropneumoniae could respond to stress hormone catecholamines, gene expression profiles after epinephrine (Epi) and norepinephrine (NE) treatment were compared with those from untreated bacteria. The microarray results showed that 158 and 105 genes were differentially expressed in the presence of Epi and NE, respectively. These genes were assigned to various functional categories including many virulence factors. Only 18 genes were regulated by both hormones. These genes included apxIA (the ApxI toxin structural gene), pgaB (involved in biofilm formation), APL_0443 (an autotransporter adhesin) and genes encoding potential hormone receptors such as tyrP2, the ygiY-ygiX (qseC-qseB) operon and narQ-narP (involved in nitrate metabolism). Further investigations demonstrated that cytotoxic activity was enhanced by Epi but repressed by NE in accordance with apxIA gene expression changes. Biofilm formation was not affected by either of the two hormones despite pgaB expression being affected. Adhesion to host cells was induced by NE but not by Epi, suggesting that the hormones affect other putative adhesins in addition to APL_0443. This study revealed that A.
    [Show full text]
  • Prokaryotic Genome Regulation: a Revolutionary Paradigm
    No. 9] Proc. Jpn. Acad., Ser. B 88 (2012) 485 Review Prokaryotic genome regulation: A revolutionary paradigm † By Akira ISHIHAMA*1, (Communicated by Tasuku HONJO, M.J.A.) Abstract: After determination of the whole genome sequence, the research frontier of bacterial molecular genetics has shifted to reveal the genome regulation under stressful conditions in nature. The gene selectivity of RNA polymerase is modulated after interaction with two groups of regulatory proteins, 7 sigma factors and 300 transcription factors. For identification of regulation targets of transcription factors in Escherichia coli, we have developed Genomic SELEX system and subjected to screening the binding sites of these factors on the genome. The number of regulation targets by a single transcription factor was more than those hitherto recognized, ranging up to hundreds of promoters. The number of transcription factors involved in regulation of a single promoter also increased to as many as 30 regulators. The multi-target transcription factors and the multi-factor promoters were assembled into complex networks of transcription regulation. The most complex network was identified in the regulation cascades of transcription of two master regulators for planktonic growth and biofilm formation. Keywords: transcription regulation, genome regulation, transcription factor, regulation network, genomic SELEX, Escherichia coli developed and employed to reveal the expression of 1. Introduction the whole set of genes on the genome (the tran- In the early stage of molecular biology, Esche- scriptome) under a given culture condition. The high- richia coli served as a model organism of biochemical, throughput microarray has made a break-through biophysical, molecular genetic and biotechnological for providing transcription patterns of the whole set studies.
    [Show full text]
  • Full Text Document (Pdf)
    Kent Academic Repository Full text document (pdf) Citation for published version Dolata, Katarzyna M. and Montero, Isabel Guerrero and Miller, Wayne and Sievers, Susanne and Sura, Thomas and Wolff, Christian and Schlüter, Rabea and Riedel, Katharina and Robinson, Colin (2019) Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses. Microbiological Research, 218 . pp. 97-107. ISSN 0944-5013. DOI https://doi.org/10.1016/j.micres.2018.10.008 Link to record in KAR https://kar.kent.ac.uk/70187/ Document Version Publisher pdf Copyright & reuse Content in the Kent Academic Repository is made available for research purposes. Unless otherwise stated all content is protected by copyright and in the absence of an open licence (eg Creative Commons), permissions for further reuse of content should be sought from the publisher, author or other copyright holder. Versions of research The version in the Kent Academic Repository may differ from the final published version. Users are advised to check http://kar.kent.ac.uk for the status of the paper. Users should always cite the published version of record. Enquiries For any further enquiries regarding the licence status of this document, please contact: [email protected] If you believe this document infringes copyright then please contact the KAR admin team with the take-down information provided at http://kar.kent.ac.uk/contact.html Microbiological Research 218 (2019) 97–107 Contents lists available at ScienceDirect Microbiological Research journal homepage: www.elsevier.com/locate/micres Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses T Katarzyna M.
    [Show full text]
  • Transcription Regulation of the Expression of the Plasmid Encoded Toxin of Enteroaggregative Escherichia Coli
    TRANSCRIPTION REGULATION OF THE EXPRESSION OF THE PLASMID ENCODED TOXIN OF ENTEROAGGREGATIVE ESCHERICHIA COLI by Jacob Henry Duddy A thesis submitted to The University of Birmingham for the degree of MASTER OF PHILOSOPHY February 2013 School of Biosciences The University of Birmingham University of Birmingham Research Archive e-theses repository This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. Abstract The pathogenic properties of Enteroaggregative Escherichia coli strain 042 results from the synchronised expression of virulence factors, which include the Plasmid Encoded Toxin. Pet is a member of the serine protease autotransporter of the Enterobacteriaceae family and contributes to infection by cleaving α-fodrin, disrupting the actin cytoskeleton of host cells. The expression of Pet is induced by global transcription factor CRP with further enhancement by the nucleoid associated protein Fis. This study identifies the residues of RNA polymerase, Fis and CRP required for the induction of transcription, thereby clarifying the mechanism of activation employed by the transcription factors. Fis activates transcription from the Fis binding site via a direct interaction with RNA polymerase, facilitated by protein specific determinants. This interaction is dependent on the position of the Fis binding site on the DNA and it subsequent orientation on the helical face of the DNA.
    [Show full text]
  • La Réponse Au NO Au Centre De La Pathogenèse Bactérienne Et Cible D’Antibiotiques Innovants Constance Porrini
    La réponse au NO au centre de la pathogenèse bactérienne et cible d’antibiotiques innovants Constance Porrini To cite this version: Constance Porrini. La réponse au NO au centre de la pathogenèse bactérienne et cible d’antibiotiques innovants. Médecine humaine et pathologie. Université Paris-Saclay, 2020. Français. NNT : 2020UP- ASA008. tel-03175216 HAL Id: tel-03175216 https://pastel.archives-ouvertes.fr/tel-03175216 Submitted on 19 Mar 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. La réponse au NO au centre de la pathogenèse bactérienne et cible d’antibiotiques innovants Thèse de doctorat de l'université Paris-Saclay École doctorale n°581, Agriculture, Alimentation, Biologie, Environnement, Santé - ABIES Spécialité de doctorat : Microbiologie INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France Référent : AgroParisTech Thèse présentée et soutenue à Paris-Saclay, le 12 juin 2020, par Constance PORRINI Composition du Jury Stéphanie BURY-MONE Présidente Professeure, Université Paris-Saclay Véronique BROUSSOLLE Rapporteur et
    [Show full text]
  • Regulation Des Virulenzgenregulators Slya in Pathogenen Und Apathogenen Escherichia Coli Stämmen
    Regulation des Virulenzgenregulators SlyA in pathogenen und apathogenen Escherichia coli Stämmen Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades einer Doktorin der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Anna-Katharina Wagner aus Berlin 1. Referentin: Professor Dr. Petra Dersch 2. Referent: Professor Dr. Michael Steinert eingereicht am: 13.09.2010 mündliche Prüfung (Disputation) am: 24.01.2011 Druckjahr 2011 Für meine Eltern Danksagung Mein besonderer Dank gilt Prof. Dr. Petra Dersch für die Bereitstellung des interessanten Themas, die gute Betreuung und die vielen wertvollen Ratschläge und Ideen. Bei Prof. Dr. Michael Steinert möchte ich mich herzlich für die Übernahme des zweiten Gutachtens bedanken. Vor allem danke ich auch Maurice Scheer für die Hilfe bei der statistischen Auswertung der Microarray-Daten und Manfred Rohde für die großartigen elektronenmikroskopischen Aufnahmen. Natürlich danke ich auch besonders den Mitgliedern meiner Arbeitsgruppe, die zu meiner Arbeit beigetragen haben, insbesondere Ann Katrin Heroven, Henriette Langhans, Katrin Benson, Wiebke Opitz und Katja Böhme und allen jetzigen und ehemaligen Laboranten der AG Dersch und AG Jahn für die schöne Zeit und die nette Arbeitsatmosphäre, für Anregungen, Motivation und konstruktive Kritik. Für die großzügige Bereitstellung von Stämmen und Plasmiden danke ich Prof. Dr. Regine Hengge und Prof. Dr. Herbert Schmidt. Meinem gutmütigen, liebevollen Partner danke ich für sein Verständnis, seine Geduld und gute Laune und für die vielen, vielen Botengänge, ohne die der Abschluss dieser Arbeit aus der Ferne nicht möglich gewesen wäre. Abkürzungsverzeichnis Abkürzungsverzeichnis µg Microgramm µl Microliter µM Micromolar µmol Micromol A Adenin Amp Ampere Abb.
    [Show full text]
  • Estudi De L'expressió I Funció De Les Ribonucleotidil Reductases D'escherichia Coli
    Estudi de l'expressió i funció de les Ribonucleotidil Reductases d'Escherichia coli María del Mar Cendra Gascón ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tdx.cat) i a través del Dipòsit Digital de la UB (diposit.ub.edu) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX ni al Dipòsit Digital de la UB. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX o al Dipòsit Digital de la UB (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tdx.cat) y a través del Repositorio Digital de la UB (diposit.ub.edu) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR o al Repositorio Digital de la UB.
    [Show full text]