Isolation and Characterization of Native Microalgae from the Peruvian Amazon with Potential for Biodiesel Production
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Phylogenetic Placement of Botryococcus Braunii (Trebouxiophyceae) and Botryococcus Sudeticus Isolate Utex 2629 (Chlorophyceae)1
J. Phycol. 40, 412–423 (2004) r 2004 Phycological Society of America DOI: 10.1046/j.1529-8817.2004.03173.x PHYLOGENETIC PLACEMENT OF BOTRYOCOCCUS BRAUNII (TREBOUXIOPHYCEAE) AND BOTRYOCOCCUS SUDETICUS ISOLATE UTEX 2629 (CHLOROPHYCEAE)1 Hoda H. Senousy, Gordon W. Beakes, and Ethan Hack2 School of Biology, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK The phylogenetic placement of four isolates of a potential source of renewable energy in the form of Botryococcus braunii Ku¨tzing and of Botryococcus hydrocarbon fuels (Metzger et al. 1991, Metzger and sudeticus Lemmermann isolate UTEX 2629 was Largeau 1999, Banerjee et al. 2002). The best known investigated using sequences of the nuclear small species is Botryococcus braunii Ku¨tzing. This organism subunit (18S) rRNA gene. The B. braunii isolates has a worldwide distribution in fresh and brackish represent the A (two isolates), B, and L chemical water and is occasionally found in salt water. Although races. One isolate of B. braunii (CCAP 807/1; A race) it grows relatively slowly, it sometimes forms massive has a group I intron at Escherichia coli position 1046 blooms (Metzger et al. 1991, Tyson 1995). Botryococcus and isolate UTEX 2629 has group I introns at E. coli braunii strains differ in the hydrocarbons that they positions 516 and 1512. The rRNA sequences were accumulate, and they have been classified into three aligned with 53 previously reported rRNA se- chemical races, called A, B, and L. Strains in the A race quences from members of the Chlorophyta, includ- accumulate alkadienes; strains in the B race accumulate ing one reported for B. -
TRADITIONAL GENERIC CONCEPTS VERSUS 18S Rrna GENE PHYLOGENY in the GREEN ALGAL FAMILY SELENASTRACEAE (CHLOROPHYCEAE, CHLOROPHYTA) 1
J. Phycol. 37, 852–865 (2001) TRADITIONAL GENERIC CONCEPTS VERSUS 18S rRNA GENE PHYLOGENY IN THE GREEN ALGAL FAMILY SELENASTRACEAE (CHLOROPHYCEAE, CHLOROPHYTA) 1 Lothar Krienitz2 Institut für Gewässerökologie und Binnenfischerei, D-16775 Stechlin, Neuglobsow, Germany Iana Ustinova Institut für Botanik und Pharmazeutische Biologie der Universität, Staudtstrasse 5, D-91058 Erlangen, Germany Thomas Friedl Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Abteilung Experimentelle Phykologie und Sammlung von Algenkulturen, Universität Göttingen, Untere Karspüle 2, D-37037 Göttingen, Germany and Volker A. R. Huss Institut für Botanik und Pharmazeutische Biologie der Universität, Staudtstrasse 5, D-91058 Erlangen, Germany Coccoid green algae of the Selenastraceae were in- few diacritic characteristics and that contain only a vestigated by means of light microscopy, TEM, and small number of species) and to reestablish “large” 18S rRNA analyses to evaluate the generic concept in genera of Selenastraceae such as Ankistrodesmus. this family. Phylogenetic trees inferred from the 18S Key index words: 18S rRNA, Ankistrodesmus, Chloro- rRNA gene sequences showed that the studied spe- phyta, Kirchneriella, Monoraphidium, molecular system- cies of autosporic Selenastraceae formed a well- atics, morphology, Podohedriella, pyrenoid, Quadrigula, resolved monophyletic clade within the DO group of Selenastraceae Chlorophyceae. Several morphological characteris- tics that are traditionally used as generic features Abbreviations: LM, light microscopy -
Fourier Transform Infrared with Attenuated Total Reflectance Applied to the Discrimination of Freshwater Planktonic Coccoid Green Microalgae
RESEARCH ARTICLE Fourier Transform Infrared with Attenuated Total Reflectance Applied to the Discrimination of Freshwater Planktonic Coccoid Green Microalgae Guilherme Pavan de Moraes*, Armando Augusto Henriques Vieira Laboratory of Phycology, Department of Botany, Universidade Federal de Sa˜o Carlos (UFSCar), Sa˜o Carlos, Sa˜o Paulo, Brazil *[email protected] Abstract OPEN ACCESS Despite the recent advances on fine taxonomic discrimination in microorganisms, Citation: Moraes GPd, Vieira AAH (2014) Fourier Transform Infrared with Attenuated Total namely using molecular biology tools, some groups remain particularly problematic. Reflectance Applied to the Discrimination of Freshwater Planktonic Coccoid Green Fine taxonomy of green algae, a widely distributed group in freshwater ecosystems, Microalgae. PLoS ONE 9(12): e114458. doi:10. remains a challenge, especially for coccoid forms. In this paper, we propose the use 1371/journal.pone.0114458 of the Fourier Transform Infrared (FTIR) spectroscopy as part of a polyphasic Editor: Heidar-Ali Tajmir-Riahi, University of Quebect at Trois-Rivieres, Canada approach to identify and classify coccoid green microalgae (mainly order Received: August 12, 2014 Sphaeropleales), using triplicated axenic cultures. The attenuated total reflectance Accepted: October 19, 2014 (ATR) technique was tested to reproducibility of IR spectra of the biological material, a primary requirement to achieve good discrimination of microalgal Published: December 26, 2014 strains. Spectral window selection was also tested, in conjunction with the first Copyright: ß 2014 Moraes, Vieira. This is an open-access article distributed under the terms of derivative treatment of spectra, to determine which regions of the spectrum the Creative Commons Attribution License, which permits unrestricted use, distribution, and repro- provided better separation and clustering of strains. -
Freshwater Algae in Britain and Ireland - Bibliography
Freshwater algae in Britain and Ireland - Bibliography Floras, monographs, articles with records and environmental information, together with papers dealing with taxonomic/nomenclatural changes since 2003 (previous update of ‘Coded List’) as well as those helpful for identification purposes. Theses are listed only where available online and include unpublished information. Useful websites are listed at the end of the bibliography. Further links to relevant information (catalogues, websites, photocatalogues) can be found on the site managed by the British Phycological Society (http://www.brphycsoc.org/links.lasso). Abbas A, Godward MBE (1964) Cytology in relation to taxonomy in Chaetophorales. Journal of the Linnean Society, Botany 58: 499–597. Abbott J, Emsley F, Hick T, Stubbins J, Turner WB, West W (1886) Contributions to a fauna and flora of West Yorkshire: algae (exclusive of Diatomaceae). Transactions of the Leeds Naturalists' Club and Scientific Association 1: 69–78, pl.1. Acton E (1909) Coccomyxa subellipsoidea, a new member of the Palmellaceae. Annals of Botany 23: 537–573. Acton E (1916a) On the structure and origin of Cladophora-balls. New Phytologist 15: 1–10. Acton E (1916b) On a new penetrating alga. New Phytologist 15: 97–102. Acton E (1916c) Studies on the nuclear division in desmids. 1. Hyalotheca dissiliens (Smith) Bréb. Annals of Botany 30: 379–382. Adams J (1908) A synopsis of Irish algae, freshwater and marine. Proceedings of the Royal Irish Academy 27B: 11–60. Ahmadjian V (1967) A guide to the algae occurring as lichen symbionts: isolation, culture, cultural physiology and identification. Phycologia 6: 127–166 Allanson BR (1973) The fine structure of the periphyton of Chara sp. -
I Universidade Federal De São Carlos Centro De Ciências Biológicas E Da Saúde Programa De Pós-Graduação Em Ecologia E
UNIVERSIDADE FEDERAL DE SÃO CARLOS CENTRO DE CIÊNCIAS BIOLÓGICAS E DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM ECOLOGIA E RECURSOS NATURAIS Biodiversidade de Selenastraceae (Sphaeropleales, Chlorophyceae): características morfológicas e sequenciamento dos marcadores moleculares 18S rDNA, rbcL e ITS como base taxonômica tradicional. Thaís Garcia da Silva Tese apresentada ao Programa de Pós-Graduação em Ecologia e Recursos Naturais da Universidade Federal de São Carlos, como parte dos requisitos para obtenção do título de DOUTOR EM CIÊNCIAS, área de concentração: ECOLOGIA E RECURSOS NATURAIS. Orientador: Prof. Dr. Armando Augusto Henriques Vieira São Carlos – SP 2016 I Ficha catalográfica elaborada pelo DePT da Biblioteca Comunitária UFSCar Processamento Técnico com os dados fornecidos pelo(a) autor(a) Silva, Thaís Garcia da S586b Biodiversidade de Selenastraceae (Sphaeropleales, Chlorophyceae): características morfológicas e sequenciamento dos marcadores moleculares 18S rDNA, rbcL e ITS como base taxonômica tradicional / Thaís Garcia da Silva. -- São Carlos : UFSCar, 2016. 148 p. Tese (Doutorado) -- Universidade Federal de São Carlos, 2016. 1. Taxonomia. 2. Sistemática molecular. 3. Selenastraceae. 4. Biodiversidade. 5. Filogenia. I. Título. III “Longe se vai sonhando demais Mas onde se chega assim? Vou descobrir o que me faz sentir Eu, caçador de mim.” Milton Nascimento IV Agradecimentos Primeiramente, ao Dr. Armando Augusto Henriques Vieira por ter me aceitado como estagiária e aluna de doutorado. Agradeço imensamente por todo apoio e dedicação nesses 6 anos de trabalho no Laboratório de Ficologia, me mostrando novas possibilidades de aprendizado e estimulando o meu caminhar na pesquisa e conhecimento na ficologia. Sinto- me imensamente honrada por ter trilhado um caminho tão importante de minha vida sob sua tutoria e me espelho no seu exemplo de verdadeiro amor e dedicação à profissão. -
Inventory of Microalgae at Sepanjang Beach the Coastal Area of Gunung Kidul Jogjakarta
p-ISSN: 2477-3328 International Summit on Science Technology and Humanity (ISETH 2018) e-ISSN: 2615-1588 Integrating Knowledge for Future Sustainable Development Inventory of microalgae at Sepanjang Beach the coastal area of Gunung Kidul Jogjakarta Efri Roziaty1; Universitas Muhammadiyah Surakarta, Indonesia Shohifa Aulia Akbar; Universitas Muhammadiyah Surakarta, Indonesia Abstract: Research conducted in the coastal area along Sepanjang Beach Gunung Kidul Jogjakarta aims to find out what types of microalgae are present in the region. The area of Sepanjang Beach Gunung Kidul Jogjakarta is one of the most favourite beach visited by tourists both domestic and foreign. The research was conducted in March - October 2018. The sampling method used was Purpossive Sampling where it was determined as many as 5 sampling points called the Station. Then at each station created 2 substations are made as replications. From the results of the study found as many as 12 species from 4 families namely Bacillariophyceae, Chlorophyceae, Euglenophyceae and Cyanophyceae. Of the four families produced 12 species including Navicula sp., Euglena viridis, Spirulina sp. and Oscillatoria sp. It indicates that the environment is still good. Keywords : Sepanjang Beach, Gunung Kidul Jogjakarta, microalgae, Protists, microorganism Introduction Microalgae commonly known as phytoplankton (Abubakar, Mutie, & Kenya, 2011) is an organism is a food from higher organisms, namely aquatic herbivores like herbivorous fish and invertebrates. Microalgae habitat is both fresh and marine waters. Microalgae is a part of primary productivity in watering ecosystem (freshwater and also marine). Some microalgae divide into several groups according the habitat they’re found, their terms such as bioflim, benthos, periphyton and epilithon (epilitic) (Al-Harbi, 2017). -
Molecular Systematics of the Green Algal Order Trentepohliales (Chlorophyta)
Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 2000 Molecular Systematics of the Green Algal Order Trentepohliales (Chlorophyta). Juan Manuel Lopez-bautista Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Lopez-bautista, Juan Manuel, "Molecular Systematics of the Green Algal Order Trentepohliales (Chlorophyta)." (2000). LSU Historical Dissertations and Theses. 7209. https://digitalcommons.lsu.edu/gradschool_disstheses/7209 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm m aster UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy subm itted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bteedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. -
낙동강 하구역 식물플랑크톤 생물상 변동과 출현종 고찰 Literature Survey on the Phytoplankton Flora In
Original Article 한수지 46(4), 467-487, 2013 Kor J Fish Aquat Sci 46(4),467-487, 2013 낙동강 하구역 식물플랑크톤 생물상 변동과 출현종 고찰 정익교1,2*ㆍ김남림1ㆍ송은혜1ㆍ이지은2ㆍ이상래2 부산대학교 해양학과1, 부산대학교 해양연구소2 Literature Survey on the Phytoplankton Flora in the Nakdong River Estuary, Korea Ik Kyo Chung1,2*, Nam Lim Kim1 Eun Hye Song1, Jee Eun Lee2 and Sang-Rae Lee2 1Department of Oceanography, Pusan National University, Busan 609-735, Korea 2Marine Research Institute, Pusan National University, Busan 609-735, Korea Long-term and systematic monitoring of phytoplankton is important, as phytoplankton serve as indicators of water quality status in aquatic environments. In the present study, the plankton community structure of the Nakdong River estuary over the past 30 years was investigated by literature survey to provide references for the occurrence of plank- tonic species. A total of 876 species from 18 taxonomic lineages were reported and, among them, 569 species were identified to occur seasonally at the Mulgeum, Seonam and Eulsukdo sites. Diatoms and chlorophytes were dominant in the microscopic studies. The 54 species at the Mulgeum and the 96 species at the Eulsukdo sites have not been reported since the river barrage construction in 1987. The majority of reports speculate that there has been a change in the aquatic environment, particularly the plankton community structure, due to operation of a river barrage since 1987. Key Words: Phytoplankton community structure, Nakdong River estuary, Light microscopy, River barrage 서 론 모니터링이 필요하다. 낙동강은 우리나라에서 두 번째 긴 강으로 유역의 식수원, 농 플랑크톤은 바다, 강, 호수 등 수생태계의 표영계 상부에 서식 공업 용수원으로 중요 수자원으로 쓰이고 있다. -
Nine Taxa of Newly Recorded Species of Chlorophytes (Chlorophyceae and Trebouxiophyceae, Chlorophyta) in Korea
JOURNAL OF Research Paper ECOLOGY AND ENVIRONMENT http://www.jecoenv.org J. Ecol. Environ. 38(4): 637-646, 2015 Nine taxa of newly recorded species of chlorophytes (Chlorophyceae and Trebouxiophyceae, Chlorophyta) in Korea Hyun-Joo Shin,1,2 Ji-Hoon Im,1 Hyun-Cheol Jeong1 and Ok-Min Lee 1,* 1Department of Life science, College of Natural Science, Kyonggi University, Suwon 16227, Korea 2Water Environment Research Department, National Institute of Environmental Research, Environmental Research Complex, Incheon 22689, Korea Abstract In this study, we collected, identified, and cultured phytoplankton that inhabited the small-scale artificial wetlands in Danyang-gun of Chungcheungbuk-do and Suwon-si of Gyeonggi-do. From the study, nine newly recorded species, in- cluding four genera (Mychonastes, Willea, Hindakia, and Oocystella), were found in Korea: Choricystis guttula, Mychon- astes densus, Willea apiculata, Pseudokirchneriella elongata, Raphidocelis subcapitata, Hindakia tetrachotoma, Oocys- tella nephrocytioides, Oocystis bispora, and Oocystis ecballocystiformis. The morphological characteristics of the nine taxa identified in this study were mostly similar to previously reported characteristics. However,Oocystella nephrocytioides were smaller than previously recorded, while Choricystis guttula and Mychonastes densus were larger than previously recorded. Key words: Chlorophyceae, newly recorded species, phytoplankton, Trebouxiophyceae INTRODUCTION Green algae appear in diverse habitats, including fresh- and Guiry 2015). water, brackish water, and terrestrial habitats, and they Chlorophyceae and Trebouxiophyceae are usually uni- are used in ecological studies. Some species are consid- cellular or in colonies. The cell morphology and the chlo- ered important in many industries, such as biodiesel, roplast morphology are very diverse, and some species medical, food, and cosmetics (Kim and Chung 1993, Oh have one to hundreds of flagella. -
1. Resistance to Ocean Acidification in Upwelling-Associated Phytoplankton Communities Robert H. Lampe1,2, Tyler H. Coale1,2, Ki
1. Resistance to ocean acidification in upwelling-associated phytoplankton communities Robert H. Lampe1,2, Tyler H. Coale1,2, Kiefer Forsch1, Samuel Kekuewa1, Ariel Rabines1,2, Hong Zheng2, Andreas J. Andersson1, Katherine A. Barbeau1, & Andrew E. Allen1,2. 1Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093, USA 2J. Craig Venter Institute, La Jolla, CA 92037, USA Atmospheric carbon dioxide (CO2) concentrations have rapidly risen to levels that have not been observed in 800,000 years. Increased CO2 dissolving in seawater results in a decline in pH and shift in carbonate chemistry commonly referred to as ocean acidification. In particular, coastal upwelling regions are acidifying at rates double that of the global average with an anthropogenic signal superimposed on existing high natural variability. Recent laboratory studies suggest that acidification may reduce iron bioavailability and expand existing iron limitation in upwelling environments. Here we show that upwelling-associated phytoplankton communities are resistant to ocean acidification via trace metal clean incubations in the California Current Ecosystem. Natural communities were subjected to 400, 800, and 1200 ppm pCO2 and examined for changes in growth, nutrient uptake, community composition, and gene expression. Despite the dramatic decline in pH, communities dominated by diatoms and the picoeukaryotes Ostreococcus and Pelagomonas were unaffected. These results suggest that phytoplankton in upwelling regions are accustomed to low pH and that high levels of primary production will likely continue in upwelling environments under future acidification scenarios. 2. A growth ceiling exists when Prochlorococcus cultivates in artificial seawater media? Zhiying Lu & Jeffrey Morris. Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA 35294 As one of the most abundant phytoplankton in the oligotrophic ocean, Prochlorococcus contributes nearly half of primary productivity in the vast open ocean ecosystems. -
Selenastraceae (Sphaeropleales, Chlorophyceae): Rbcl, 18S Rdna and ITS-2 Secondary Structure Enlightens Traditional Taxonomy, Wi
Fottea, Olomouc, 17(1): 1–19, 2017 1 DOI: 10.5507/fot.2016.010 Selenastraceae (Sphaeropleales, Chlorophyceae): rbcL, 18S rDNA and ITS–2 secondary structure enlightens traditional taxonomy, with description of two new genera, Messastrum gen. nov. and Curvastrum gen. nov. Thaís GARCIA DA SILVA1*, Christina BOCK2, Célia Leite SANT’ANNA3, Inessa Lacati- va BAGATINI1, Sabina WODNIOK2 & Armando Augusto Henriques VIEIRA1 1Laboratory of Phycology, Department of Botany, Federal University of São Carlos, Graduate Program in Ecology and Natural Resources, CEP 13560–590, São Carlos, SP, Brazil; * Corresponding author e–mail: [email protected] 2Department of Biodiversity, University of Duisburg–Essen, Essen, D–45141, Germany 3Institute of Botany, Nucleus of Phycology, CEP 04301–902, São Paulo, SP, Brazil Abstract: The phylogeny of the family Selenastraceae was investigated by light microscopy, 18S rDNA, rbcL and ITS–2 analyses. Various morphological features traditionally used for species and genera identification were investigated. All selenastracean strains studied have naked pyrenoids within the chloroplast, except the genus Chlorolobion, which presented starch envelope. The molecular analyses showed that no morphological criterion considered so far is significant for the systematics of the Selenastraceae, but a set of features may be suitable to identify the genera Ankistrodesmus and Chlorolobion. Phylogenetic analyses showed the genera Monoraphidium, Kirchneriella and Selenastrum were not monophyletic and not distinguishable as separate -
Isolation and Characterization of Native Microalgae from the Peruvian Amazon with Potential for Biodiesel Production
Article Isolation and Characterization of Native Microalgae from the Peruvian Amazon with Potential for Biodiesel Production Marianela Cobos 1,*, Jae D. Paredes 1, J. Dylan Maddox 2,3, Gabriel Vargas-Arana 1, Leenin Flores 4, Carla P. Aguilar 4, Jorge L. Marapara 5 and Juan C. Castro 5,* 1 Laboratorio de Biotecnología y Bioenergética, Universidad Científica del Perú (UCP), Av. Abelardo Quiñones km 2.5, Iquitos 6024, Peru; [email protected] (J.D.P.); [email protected] (G.V.-A.) 2 Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum of Natural History, Chicago, IL 60605, USA; [email protected] 3 Environmental Sciences, American Public University System, Charles Town, WV 25414, USA 4 Laboratorio de Biotecnología Acuática, Instituto del Mar del Perú (IMARPE), Esquina Gamarra y General Valle S/N Chucuito, Callao 07021, Peru; [email protected] (L.F.); [email protected] (C.P.A.) 5 Unidad Especializada de Biotecnología, Centro de Investigaciones de Recursos Naturales de la Amazonía (CIRNA), Universidad Nacional de la Amazonía Peruana (UNAP), Psje. Los Paujiles S/N, Iquitos 16024, Peru; [email protected] * Correspondence: [email protected] (M.C.); [email protected] (J.C.C.); Tel.: +51-065-261074 (M.C.); +51-065-263569 (J.C.C.) Academic Editor: Thomas E. Amidon Received: 10 December 2016; Accepted: 8 February 2017; Published: 14 February 2017 Abstract: Biodiesel production from microalgae triacylglycerols is growing, because this feedstock is a more sustainable and advantageous alternative. In this study, we isolated and identified fourteen strains of native microalgae from the Peruvian Amazon. These strains showed great heterogeneity in biomass productivity, lipid productivity and lipid content, and thus, three of them (Acutodesmus obliquus, Ankistrodesmus sp.