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That the Capacity of a Plant Species to Adjust Itself, Due to a Change N Environmental Temperature, Is Genetically Controlled

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That the Capacity of a Plant Species to Adjust Itself, Due to a Change N Environmental Temperature, Is Genetically Controlled 122 that the capacity of a plant species to adjust itself, due to a change n environmental temperature, is genetically controlled. The différences in physiological adjustments among genotypes are P^i'"^'^;^^',^" ,^^^^,f,\P^^'^ of heat acclimation (heat adaptability) rather than ^", \^^\ \"^^^r^^^^^ ^^^î tolerance. This adaptive character, which protects plants against heat stress, works only if the heat adaptability can occur rapidly (J), so that the damage would not occur, which in turn results in better crop performance. References 1. Kigel, J. and M. Ofir. 1981. The morphogenetic processes that determine yield in french bean. Reports to A.R.O., Ministry of Agri., Israel. 2. Chen,\^ii\.M, ii.ii.,H.H., Z.Y.A.. I. oiiciiShen aiiuand r.M.P.H. ui.Li. 1982.• ^^i-» ,^—~^~~Adaptability ___X.__ of crop plants to high temperature stress. Crop Sei., 22:719-725. 3. Shen, Z.Y. and P.H. Li. 1982. Heat adaptability of the tomato. HortSci. 17:924-925. ««»«« INTERNAL CONTAMINATION OF BLACK BEAN SEED LOTS WITH XANTHOMONAS BLIGHT BACTERIA FROM DIFFERENT STATES OF VENEZUELA Nancy Contreras de Velasquez and Gustavo Trujillo Escuela de Postgrado, Facultad de Agronomía Universidad Central de Venezuela, Maracay Venezuela To evaluate the internal bacterial contamination in black bean seed lots, a survey was conducted with 176 samples from different classes of seed' such as: genetic, foundation, registered, certified, and harvested from growers and experiments. The survey came from different States of the Country Anzoategui, Apure, Aragua, Carabobo, Guarico, Lara, Monagas, Portuguesa, Sucre, Trujillo and Yaracuy. The samples of 80 g from each lot were weigthed, the surface was sterilized during 2 minutes with 2,6% NaOCl, rinsing twice in sterile wa ter and incubated in a shaker for 48 hr in the following enrichment medium (SEM): 1,0 g yeast extract, 25 mg cycloheximide, 2 mg nitrofurantoin, 1 mg nalidixic acid in 1000 ml 0,01 phosphate buffer pH 7.2. The bacteria were then sedimented from the liquid surrounding the seed by 15 minutes centrifugation at 5000 g, resuspended in 1 ml buffered saline steamed 60 min at 1Ü0°C, and tested serologically, using Ouchterlony Technique, more detail about SEM (2). Antisera to Xanthomonas blight isolates at titers of 1:3000 - 1:5000 A r^^A in rabbits by intravenous inyection of formalin killed were produced xn »-a , ,, ».^i ^ /^N cells following Trujillo and Saettler procedure (3). 123 Ninety-three of the one hundred seventy-six black bean samples tested, showed positive results for internal blight contamination with the SEM-serology procedures the blight bacteria was detected in the States Anzoategui, Apure, Aragua, Carabobo, Monagas, Portuguesa, Sucre y Trujillo. In the States Apure, Monagas, Sucre and Trujillo the blight bacteria has been found for the first time (1). The results indicated the necessity to do a similar study for the halo blight bacteria, (Pseudomonas phaseolicola), and to use an adecúate technique to detect the blight bacteria in the phytopathological analysis for bean seed, used in the Seed Certification Program in Venezuela. Table 1: Detection of internal bacterial blight contamination in black bean seed lots of different seed classes in Venezuela*. Class of seed Samples Samples wi th Percentage of number blight bl igh ted samples Genetic 4 4 100 Registered 9 8 89 Foundation 1 0 0 Certified 45 14 31 Harvested from farmer 64 40 63 Harvested from experiments 53 27 51 * Semiselective media and Ouchterlony Technique was used. References : 1) ALBARRACIN M., G. E. TRUJILLO, and 0. BORGES. 1982. "La quemazón bacte- riana de la caráota, (Phaseolus vulgaris L.), en Venezuela". Rev. Fac. Agron. (Maracay) XII:213-225. 2) TRUJILLO, G. E., and A. W. SAETTLER. 1979. "A combined semiselective medium and serology test for the detection of Xanthomonas blight bacteria in bean seed". Journal of Seed Technology 4:35-41. 3) TRUJILLO, G. E., and A. W. SAETTLER. 1979. "Production and characteriza- tion of Antisera of Xanthomonas phaseoli and X^ phaseoli var. fuscans" Research Report 414. Mich. State University. Experiment Station. East. Lansines. ft»«»» LINKAGE BETWEEN THE GENE CODING THE SMALL SÜBÜNIT OF RIBÜLCSE BISPHOSPHATE CARBOXYLASE AND THE GENE CODING MALIC ENZÏME IN PHASEOLUS VULGARIS N. F. Weeden Department of Horticultural Sciences New York State Agricultural Experiment Station Geneva, New York 1^1^156 An F2 population derived from the cross 'Seafarer» x 'Pirate* was analyzed for segregation in H isozyme systems: adenylate kinase (AdK), shikimic dehydrogenase (SKDH), ribulóse bisphosphate carboxylase (rubisco), and malic enzyme (ME). The F2 population was grown.in the greenhouse at .
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