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“Aronia Berries: Extraction and Characterization of Valuable Compounds”

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“Aronia Berries: Extraction and Characterization of Valuable Compounds” E.Zikou1, S.Santzouk1*, G.Santzouk1, M. Errico2, C. Pastore3 and L. di Bitonto3 1Santzouk Samir and Co. General Partnership, PANAX, Chrissostomou Smirnis 14, Agios Konstantinos, Aetoloacarnania, GR30100, Greece 2.University of Southern Denmark, Department of Chemical Engineering, Biotechnology and Environmental Technology, Campusvej 55, 5230 Odense M, Denmark 3 Consiglio Nazionale delle Ricerche (CNR), Istituto Di Ricerca sulle Acque (IRSA), Viale de Blasio 5, 70132 Bari, Italy “Aronia Berries: Extraction and characterization of valuable compounds” Funded by the European Union 1 Origin Deciduous shrubs Rosaceae family Eastern North America 2 Europe variates Balkan Bulgaria, Serbia, FYROM, etc. Middle-European(Viking) Poland, Germany, Belgium, France, etc. Euro-Asiatic (Nero) Russia, Finland, Sweden, etc. 3 Aronia species Red chokeberry- Aronia arbutifolia Purple chokeberry- Aronia prunifolia Black chokeberry- Aronia Green chokeberry- melanocarpa Sorbaronia mitschurinii 4 Melanocarpa (Black Chokeberry)- Photinia melanocarpa 1m tall – 3m wide Small leaves (< 6cm wide) White flowers Black fruit (6-9 mm wide) Persisting into winter (up to -40 °C) 5 Exploitable parts Fruits Leaves 6 Chemical components nutrients Organic acids Vitamins Trace Phytochemicals elements Glucose l-Malic acid Vitamin C Na β-Carotene Fructose Citric acid Folates (B9) Κ Phenolics Sorbitol Isocitric acid Vitamin B1, Β2, Β6 Ca Amygdalin Pectins Shikimic acid Niacin (Β3) Μg Succinic acid Pantothenic acid Fe (Β5) Zn I 7 Pharmaceutical use Antioxidant, antimicrobial and antifungal properties Anti-inflammatory action Enhancement of the defense system (strong antioxidant) against degenerative diseases e.g. forms of cancer-neoplasias Positive effect on cardiovascular disease, cholesterol and hypertension lowering Prevention and treatment of urological diseases Cell rejuvenation (memory enhancement) Diabetes Treatment of aphrodisiac and skin diseases Hepato-protective effects Protection against radioactivity 8 No undesirable or toxic side effects Procyanidins Phenolic Anthocyanins compounds Flavonols 9 Manufacturing process of mother tinctures of plant origin: Raw material: Aronia melanocarpa Source: Greece, Denmark Μethod of exploitation: extraction-percolation Method of analysis: TLC, HPLC 10 Steps until plant extract 11 Choice of the most suitable soil: 1 • Only natural fertilization • No chemical fertilizers • No pesticide insecticides • No fungicides • No herbicides 12 2 Plant collection based on the specific collection rules: • The harvest (August-mid September) takes place late in the morning to avoid moisture • Completely dry parts of the plant • Very clean raw material (no parasites, no illnesses) • Meticulous cleaning of all kinds of impurities • Transplantation time < 48h • Use of well-ventilated packagings (perforated bags, baskets of reeds, boxes full of holes e.tc.) • Place a specific ID-information card 13 ID-information card Supplier: Name of the plant: Place of collection: Time of collection: Date of collection: Weight (kg): 14 1. Fresh fruits 2. Segmentation into alcohol vapor 3. Liquid extraction 3 Processing methods: 1. To drying 2. Determination of dry residue using a sample to determine the moisture content of the plant, which helps to calculate the degree of alcohol 3. Drying at 50°C for 24hr 15 4 Drying 16 5 Εxtraction - Percolation: ➔ separation of active ingredients from inactive ➔ isolation or collection thereof in the pure state ➔ final formatting 17 Ρrocedure 1. Ρreparation of the drip 2. Humidification to inflate with water-alcoholic solvent 3. Stack the drip into the percolator – add solvent 4. Maceration for at least 48hr 5. Receive extract 6. Let it calm for 24 hr 7. Filtration 18 6 Final control of the extract • Determination of degree of alcohol and degree of acidity (ph-meter). • Determination of crude fiber, coefficient of expansion, bitterness limit. • Immediate anthocyanin identification (therapeutic value) ➔ identification of aronia. 19 Preparations Dried fruits • Capsules • Tabletes Powder • Drops • Powder Liquid extract • Elixir 20 Νon-toxic! Our products: 21 Acknowledgement: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 778168 22 Thank you! http://www.panax-med.gr 23.
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