DOCSLIB.ORG

Curriculum Vitae (PDF)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

ANNA E. WHITFIELD Department of Plant Pathology [email protected] Kansas State University Phone:(785) 532-3364 Manhattan, Kansas 66506 FAX: (785) 532-5692 EDUCATION Ph.D. (Plant Pathology) University of Wisconsin, Madison, 2004 Advisor: Thomas L. German Dissertation Title: Virus acquisition by thrips: the role of the Tomato spotted wilt virus (TSWV) glycoproteins. M.S. (Plant Pathology) University of California, Davis, 1999 Advisor: Diane E. Ullman Thesis Title: The development of a serologically-based indexing program for Tomato spotted wilt virus in dormant Ranunculus asiaticus tubers. B.S.A. (Biological Science) University of Georgia, Athens, 1996 POSITIONS HELD 2011- Associate Professor, Department of Plant Pathology, Kansas State University, Manhattan. 2006-11 Assistant Professor, Department of Plant Pathology, Kansas State University, Manhattan. 2005-06 Postdoctoral Research Fellow, Department of Entomology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Supervisor: S.A. Hogenhout. 2004-05 Postdoctoral Researcher, Department of Entomology, University of Wisconsin, Madison Supervisor: T. L. German. 1998-03 Graduate Research Assistant, Department of Plant Pathology, University of Wisconsin, Madison. 1996-98 Graduate Research Assistant, Departments of Entomology and Plant Pathology, University of California, Davis. REFEREED PUBLICATIONS Montero-Astúa, M., Ullman, D.E., and Whitfield, A.E. Dynamics of TSWV infection and spread in its insect vector, Frankliniella occidentalis, with an emphasis on the principal salivary glands. (Manuscript written and in review by colleagues). Barandoc-Alviar K., Ramirez, G.M., Rotenberg, D. and Whitfield, A.E. Analysis of acquisition and titer of Maize mosaic rhabdovirus in its vector, Peregrinus maidis (Hemiptera: Delphacidae). (Submitted). Rotenberg, D., Bockus, W. W., Whitfield, A.E., Hervey, K., Baker, K., Ou, Z., Laney, A., De Wolf, E.D., and Appel J.A. Occurrence of Barley yellow dwarf virus and Wheat streak mosaic virus and their contributions to yield losses in winter wheat fields in Kansas. (Submitted). Anna E. Whitfield 1 curriculum vitae Rotenberg, D., Jacobson, A., Schneweis, D., and Whitfield, A.E. 2015. Thrips transmission of tospoviruses. Current Opinion in Virology. (In press). Whitfield, A.E., and Rotenberg, D. 2015. Disruption of insect transmission of plant viruses. Current Opinion in Insect Science 8:79-87. Whitfield, A.E., Falk, B.W, Rotenberg, D. Insect vector-mediated transmission of plant viruses. Virology 479-480:278-289. *Invited review for the 60th Anniversary Issue of Virology. Badillo-Vargas, I., Rotenberg, D., Schneweis, B.A., and Whitfield, A.E. 2015. RNA interference tools for Frankliniella occidentalis. Journal of Insect Physiology 76:36-46. Whitfield, A.E., Rotenberg, D., and German, T. L. 2014. Plant pest destruction goes viral. Nature Biotechnology 32:65-66. Stafford-Banks, C.A., Rotenberg, D.; Johnson, B.R., Whitfield, A.E., and Ullman, D.E. 2014. Analysis of the salivary gland transcriptome of Frankliniella occidentalis. PLoS ONE 9:e94447. Montero-Astúa, M., Rotenberg, D., Leach, A., Schneweis, B., Park, K., Park, S.H., German, T.L., and Whitfield, A.E. 2014. Disruption of vector transmission by a plant-expressed viral glycoprotein. Molecular Plant-Microbe Interactions 27:296-304. Dietzgen, R.G., Kuhn, J.H., Clawson, A.N., Freitas-Astúa, J., Goodin, M.M., Kitajima, E., Kondo, H., Wetzel, T., Whitfield, A.E. 2014. Dichorhavirus: a proposed new genus for Brevipalpus mite- transmitted, nuclear, bacilliform, bipartite, negative-strand RNA plant viruses. Archives of Virology 159:607-619. Alexander, H. M., Mauck, K. E., Whitfield, A.E., Garrett K. A., and C. M. Malmstrom. 2014. Plant- virus interactions and the agro-ecological interface. 2014. European Journal of Plant Pathology 138:529- 547. Yao, J., Rotenberg, D., Afsharifar, A., Barandoc-Alviar K., Whitfield, A.E. 2013. Development of RNAi methods for Peregrinus maidis, the corn planthopper. PLoS ONE 8(8):e70243. Nachappa, P., Margolies, D.C., Nechols, J.R., Whitfield, A.E., and Rotenberg, D. 2013. Tomato spotted wilt virus benefits a non-vector arthropod, Tetranychus urticae, by modulating different plant responses in tomato. PLoS ONE 8(9):e75909. Jacobson, A.L., Johnston, .S. Rotenberg, D., Whitfield, A.E., Booth, W., Vargo, E.L., Kennedy, G.G 2013. Genome size and ploidy of Thysanoptera. Insect Molecular Biology 22: 12-17. Elbeaino, T., Whitfield. A.E., Sharma, M., Digiaro, M. 2013. Emaravirus-specific degenerate PCR primers allowed the identification of partial RNA-dependent RNA polymerase sequences of Maize red stripe virus and Pigeonpea sterility mosaic virus. Journal of Virological Methods 188:37-40. Badillo-Vargas, I., Rotenberg, D., Schneweis, D. J., Hiromasa, Y., Tomich, J. M., and Whitfield, A.E. 2012. Proteomic analysis of the first instar larvae of Frankliniella occidentalis, the insect vector of Tomato spotted wilt virus. Journal of Virology 86:8793-8809. Whitfield, A.E., Rotenberg, D., Aritua, V., and Hogenhout, S.A. 2011. Analysis of expressed sequence tags from Maize mosaic rhabdovirus-infected gut tissues of Peregrinus maidis reveals the presence of key components of insect innate immunity. Insect Molecular Biology 20:225-242. Anna E. Whitfield 2 curriculum vitae Murugan, M., Cardona, P. Sotelo, Duraimurugan, P., Whitfield, A. E., Schneweis, D., Starkey, S., Smith, C. M. 2011. Wheat Curl Mite Resistance: Interactions of Mite Feeding With Wheat Streak Mosaic Virus Infection. Journal of Economic Entomology 104:1406-1414. Rotenberg, D., and Whitfield, A.E. 2010. Analysis of expressed sequence tags for Frankliniella occidentalis, the western flower thrips. Insect Molecular Biology 19:537-551. Rotenberg, D., Kumar, N.K.K., Ullman, D.E., Montero-Astúa, M., Willis, D.K, German, T.L., and Whitfield, A.E. 2009.Variation in Tomato spotted wilt virus titer in Frankliniella occidentalis and its association with frequency of transmission. Phytopathology 99:404-410. Ammar, E.D., Tsai, C.W., Whitfield, A.E., Redinbaugh, M.G., and Hogenhout,S.A. 2009. Cellular and molecular aspects of rhabdovirus interactions with insect and plant hosts. Annual Review of Entomology 54:447-468. Whitfield, A.E., Kumar, N.K.K., Rotenberg, D., Ullman, D.E., Wyman, E.A., Zeitlow, C., Willis, D.K., and German T.L. 2008. A soluble form of the Tomato spotted wilt virus (TSWV) glycoprotein GN (GN-S) inhibits transmission of TSWV by Frankliniella occidentalis. Phytopathology 98:45-50. Hogenhout,S.A., Ammar, E.D., Whitfield, A.E., and Redinbaugh, M.G. 2008. Insect vector interactions with persistently transmitted viruses. Annual Review of Phytopathology 46:327-359. Rotenberg, D., Jimenez Wells, A., Chapman, E.J., Whitfield, A.E., Goodman, R.M., and Cooperband, L.R. 2007. Soil properties associated with organic matter-mediated bean root rot suppression in field soil amended with fresh and composted paper mill residuals. Soil Biology and Biochemistry 39:2936-2948. Whitfield, A.E., Ullman, D.E., and German T.L. 2005. Tomato spotted wilt virus glycoprotein GC is cleaved at acidic pH. Virus Research 110:183-186. Whitfield, A.E., Ullman, D.E., and German T.L. 2005. Tospovirus-Thrips Interactions. Annual Review of Phytopathology 43:459-489. Ullman, D.E., Whitfield A.E., and German, T.L. 2005. Thrips and tospoviruses come of age: mapping determinants of insect transmission. Proceedings of the National Academy of Sciences 102:4931-4932. Whitfield, A.E., Ullman, D.E., and German T.L. 2004. Expression, purification, and characterization of a soluble form of Tomato spotted wilt virus glycoprotein GN. Journal of Virology 78:13197-13206. Whitfield, A.E., Campbell, L.R., Sherwood, J.L. and Ullman, D.E. 2003. Tissue blot immunoassay to detect tomato spotted wilt virus in Ranunculus asiaticus and other ornamentals. Plant Disease 87:618- 622. BOOK CHAPTERS Barandoc-Alviar K., Badillo-Vargas, I., and Whitfield, A.E. Interactions between insect vectors and propagative plant viruses. In Management of insect pests to agriculture: lessons learned from deciphering their genome, transcriptome and proteome. M. Ghanim and H. Czosnek, ed. Springer. (In press) Montero-Astúa, M., Stafford, C., Badillo-Vargas, I., Rotenberg, D., Ullman, D.E., and Whitfield, A. E., Tospovirus -thrips biology. In Vector-Mediated Transmission of Plant Pathogens. J.K. Brown, ed. APS Press. (In press) Anna E. Whitfield 3 curriculum vitae Redinbaugh, M.G., Whitfield, A.E., and Ammar, E.D. 2012. Insect Vector Interactions and Transmission of Cereal-infecting Rhabdoviruses. In: Rhabdoviruses: Molecular Taxonomy, Evolution, Genomics, Ecology, Cytopathology and Control, R. Dietzgen and I. Kuzmin, eds. Horizon Scientific Press, London, pp. 147-163. Dietzgen, R.G., Calisher, C.H., Kurath, G., Kuzmin, I.V., Rodriguez, L.L., Stone, D.M., Tesh, R.B., Tordo, N., Walker, P.J., Wetzel, T., and Whitfield, A.E. 2012. Rhabdoviridae. Pages 686-713 in: Virus taxonomy: classification and nomenclature of viruses: Ninth Report of the International Committee on Taxonomy of Viruses. Ed: King, A.M.Q., Adams, M.J., Carstens, E.B. and Lefkowitz, E.J. San Diego: Elsevier. Ullman, D.E., Meideros, R.B., Campbell, L.R., Whitfield, A.E., Sherwood, J.L., and German T.L. 2002. Thrips as Vectors of Tospoviruses. Pages 113-140 in: Advances in Botanical Research Plant Virus Vector Interactions. R.T. Plumb and J.A. Callow, eds. Academic Press. London. Sherwood, J.L., German, T.L., Whitfield, A.E., Moyer, J.W., and Ullman, D.E. 2001. Tospoviruses. Pages 1034-1040 in: The Encyclopedia of Plant Pathology. O.C. Maloy and T.D. Murray, eds. John Wiley and Sons, Inc. Sherwood, J.L., German, T.L., Whitfield, A.E., Moyer, J.W., and Ullman, D.E. 2001. Tomato Spotted Wilt. Pages 1030-1031 in: The Encyclopedia of Plant Pathology. O.C. Maloy and T.D. Murray, eds. John Wiley and Sons, Inc. Ullman, D.E., Casey, C.A., Whitfield, A.E., Campbell, L.R., Robb, K.L., Medeiros, R.B., German, T.L., and Sherwood, J.L. 1998. Thrips and tospoviruses: Present and future strategies for management. Brighton Conference, Pests and Diseases. British Crops Protection Council.
Recommended publications
  • Hemiptera: Cicadellidae and Delphacidae) in Brazilian Maize Crops

    Hemiptera: Cicadellidae and Delphacidae) in Brazilian Maize Crops

    1470 Florida Entomologist 96(4) December 2013 ABUNDANCE AND SPECIES RICHNESS OF LEAFHOPPERS AND PLANTHOPPERS (HEMIPTERA: CICADELLIDAE AND DELPHACIDAE) IN BRAZILIAN MAIZE CROPS 1 2 2 3 CHARLES MARTINS DE OLIVEIRA , ELIZABETH DE OLIVEIRA , ISABEL REGINA PRAZERES DE SOUZA , ELCIO ALVES , 4 5 5 6 WILLIAM DOLEZAL , SUSANA PARADELL , ANA MARIA MARINO DE REMES LENICOV AND MARINA REGINA FRIZZAS 1Embrapa Cerrados. C.P. 08223, Planaltina, Brasília/DF, 73310-970, Brazil 2Embrapa Milho e Sorgo. C. P. 151, Sete Lagoas/MG, 35701970, Brazil 3DuPont do Brazil S.A, Divisão Pioneer Sementes. C.P. 1014, Itumbiara/GO, 75503971, Brazil 4Pioneer Hi-Bred International, Inc. C.P. 1014, Itumbiara/GO, 75503971, Brazil 5Facultad de Ciencias Naturales y Museo, División Entomologia, Universidad Nacional de La Plata. Paseo del Bosque s/n – La Plata (1900), Buenos Aires, Argentina 6Universidade de Brasília, Departamento de Zoologia, Instituto de Ciências Biológicas, Brasília/DF, Brazil, 70910-900 Corresponding author; E-mail: [email protected] ABSTRACT Insects in the Cicadellidae and Delphacidae families, common in grasses, are an important group of vectors of viruses and mollicutes, which cause diseases in several plant species. The goal of this study was to evaluate the abundance and species richness of Cicadellidae and Delphacidae and the presence of potential vectors of viruses and mollicutes in maize crops in Brazil. Insects were collected using sweep nets in maize crops in 48 counties of 8 states, distributed in 4 regions of Brazil in the yr 2005, 2006 and 2007, with a total of 198 samples. The collected material was screened, and the leafhoppers and planthoppers were identi- fied at the species level.
  • The Diversity and the Abundance Ofcorn Planthopper(Hemiptera: Delphacidae)Inlampung Province

    The Diversity and the Abundance Ofcorn Planthopper(Hemiptera: Delphacidae)Inlampung Province

    Journal of Physics: Conference Series PAPER • OPEN ACCESS The Diversity and the Abundance ofCorn Planthopper(Hemiptera: Delphacidae)inLampung Province To cite this article: R Hasibuan et al 2021 J. Phys.: Conf. Ser. 1751 012043 View the article online for updates and enhancements. This content was downloaded from IP address 182.1.232.20 on 28/01/2021 at 07:59 ICASMI 2020 IOP Publishing Journal of Physics: Conference Series 1751 (2021) 012043 doi:10.1088/1742-6596/1751/1/012043 The Diversity and the Abundance ofCorn Planthopper(Hemiptera: Delphacidae)inLampung Province R Hasibuan1, Y Fitriana1, S Ratih1, L Wibowo1,T N Aeny1,FX Susilo1, I G Swibawa1, and F R Lumbanraja2 1Department of Plant Protection Department, Faculty of Agriculture, University of Lampung, Indonesia 2Department ofComputer Science, Faculty, University of Lampung, Indonesia Jln. Prof. Dr. Soemantri Brojonegoro No. 1 Bandar Lampung 35145 email:[email protected] The outbreak of delphacid planthoppers has been detected across corn-growing regions in South Lampung. Survey study was conducted in three corn fields in Natar District,South Lampung Regency. In each study site, five corn plants were randomly sampled. In each sampled plant, one leaf with maximum number of planthoppers was selected for population recording. Based on the morphological identification results, there were two types of corn planthoppers attacking corn fields during sampling periods: the white bellied-planthopper, Stenocranus pacivicus Kirkaldy and Peregrinus maidis Ashmead. During sampling periods, S. pacivicus was most abundant species, while, the Peregrinus planthopper was almost undetectable. There was similar trend peak of density S. pacificus brachypters & nymph and macropters among the three corn fields.
  • The White-Bellied Planthopper (Hemiptera: Delphacidae) Infesting Corn Plants in South Lampung Indonesia

    The White-Bellied Planthopper (Hemiptera: Delphacidae) Infesting Corn Plants in South Lampung Indonesia

    J. HPT Tropika. ISSN 1411-7525 J. HPT Tropika Vol. 17, No. 1, 2017: 96 103 Vol.96 17, No. 96: – 103, Maret 2017 - THE WHITE-BELLIED PLANTHOPPER (HEMIPTERA: DELPHACIDAE) INFESTING CORN PLANTS IN SOUTH LAMPUNG INDONESIA Franciscus Xaverius Susilo1, I Gede Swibawa1, Indriyati1, Agus Muhammad Hariri1, Purnomo1, Rosma Hasibuan1, Lestari Wibowo1, Radix Suharjo1, Yuyun Fitriana1, Suskandini Ratih Dirmawati1, Solikhin1, Sumardiyono2, Ruruh Anjar Rwandini2, Dad Resiworo Sembodo1, & Suputa3 1Fakultas Pertanian Universitas Lampung (FP-UNILA) Jl. Prof. Dr. Sumantri Brojonegoro No 1, Bandar Lampung 35145 2UPTD Balai Proteksi Tanaman Pangan dan Hortikultura Provinsi Lampung Jl. H. Zainal Abidin Pagaralam No. 1D, Bandarlampung 35132 3Fakultas Pertanian Universitas Gadjah Mada Bulaksumur, Yogyakarta 55281 ABSTRACT The White-Bellied Planthopper (Hemiptera: Delphacidae) Infesting Corn Plants in South Lampung, Indonesia. Corn plants in South Lampung were infested by newly-found delphacid planthoppers. The planthopper specimens were collected from heavily-infested corn fields in Natar area, South Lampung. We identified the specimens as the white-bellied planthopper Stenocranus pacificus Kirkaldy (Hemiptera: Delphacidae), and reported their field population abundance. Key words: corn white-bellied planthopper, Lampung, Indonesia, Stenocranus pacificus. ABSTRAK Wereng Perut Putih (Hemiptera: Delphacidae) Menginfestasi Pertanaman Jagung di Lampung Selatan. Sejenis wereng ditemukan menginfestasi pertanaman jagung di Lampung Selatan, Lampung. Hama ini diidentifikasi sebagai wereng perut putih jagung, Stenocranus pacificus Kirkaldy (Hemiptera: Delphacidae). Infestasi masif hama ini terjadi pada pertanaman jagung di kawasan Natar, Lampung Selatan. Kata kunci: wereng perut putih jagung, Lampung, Indonesia, Stenocranus pacificus. INTRODUCTION 2016, respectively, from the heavily infested farmer corn fields at South Lampung. Upon quick inspection, we Corn plants are susceptible to the attacks of noted general appearance of light brown notum and planthoppers.
  • Evolution of Delphacidae (Hemiptera: Fulgoroidea): Combined-Evidence Phylogenetics Reveals Importance of Grass Host Shifts

    Evolution of Delphacidae (Hemiptera: Fulgoroidea): Combined-Evidence Phylogenetics Reveals Importance of Grass Host Shifts

    Systematic Entomology (2010), 35, 678–691 DOI: 10.1111/j.1365-3113.2010.00539.x Evolution of Delphacidae (Hemiptera: Fulgoroidea): combined-evidence phylogenetics reveals importance of grass host shifts JULIE M. URBAN1, CHARLES R. BARTLETT2 and J A S O N R . CRYAN1 1Research and Collections, Laboratory for Conservation and Evolutionary Genetics, New York State Museum, Albany, NY, U.S.A. and 2Department of Entomology and Wildlife Ecology, University of Delaware, Newark, DE, U.S.A. Abstract. The planthopper family Delphacidae is a speciose lineage of phloem- feeding insects, with many species considered as pests of economic significance on essential world food commodities (including rice, maize, wheat, barley and sugar cane). Despite their economic importance, evolutionary relationships among delphacids, particularly those within the speciose tribe Delphacini, are largely unknown. Presented here are the results of a phylogenetic investigation of Delphacidae based on DNA nucleotide sequence data from four genetic loci (18S rDNA, 28S rDNA, wingless and cytochrome oxidase I ) and 132 coded morphological characters. The resulting topologies are used to test the higher classification of Delphacidae and to examine evolutionary patterns in host–plant associations. Our results generally support the higher classifications of Delphacidae proposed by Asche, Emeljanov and Hamilton, and suggest that the rapid diversification of the Delphacini was associated with host shifts to, and within, Poaceae, and specifically from C3 to C4 grasses. Introduction infestations in 2009 reportedly occurring in Bangladesh, China, Malaysia, Philippines, Thailand and Vietnam (Heong, 2009). The insect family Delphacidae (Hemiptera: Fulgoroidea), Within Delphacidae, 85 species are recognized as economically including approximately 2100 described species, is the most significant pests, incurring damage to approximately 25 plant speciose and economically important of the ∼20 planthopper crops (Wilson & O’Brien, 1987; Wilson, 2005).
  • Tsai: Biology of Peregrinus Maidis 19 DEVELOPMENT AND

    Tsai: Biology of Peregrinus Maidis 19 DEVELOPMENT AND

    Tsai: Biology of Peregrinus maidis 19 DEVELOPMENT AND OVIPOSITION OF PEREGRINUS MAIDIS (HOMOPTERA: DELPHACIDAE) ON VARIOUS HOST PLANTS JAMES H. TSAI Fort Lauderdale Research and Education Center University of Florida, IFAS Fort Lauderdale, FL 33314 ABSTRACT The development and oviposition of Peregrinus maidis (Ashmead) (Homoptera: Delphacidae), a serious pest and the only known vector of maize stripe tenuivirus and maize mosaic rhabdovirus in tropical and subtropical areas, was studied on the fol- lowing plants in the laboratory: corn (Zea mays L. var. Saccharata ‘Guardian’), itch grass (Rottboellia exaltata L.), rice (Oryza sativa L. var. Mars, Saturn, Nato, Bellevue, Labelle, Labonnet, and Starbonnet), sorghum (Sorghum bicolor (L.) Moench var. AKS 614), goose grass (Eleusine indica (L.) Gaertn), oats (Avena sativa L.), rye (Secale ce- reale L.), gama grass (Tripsacum dactyloides L.), barnyard grass (Echinochloa crus- galli L.) and sugarcane (Saccharum officinarum L.). Peregrinus maidis nymphs did 20 Florida Entomologist 79(1) March, 1996 not develop on rye, oats, rice and sugarcane, but the adults survived for various lengths of time on these test plants. The average length of nymphal development on corn, itch grass, sorghum, goose grass, barnyard grass and gama grass was 17.20, 17.87, 20.21, 24.97, 27.24 and 60.50 days, respectively. Adult longevity (X ± SD) on corn, gama grass, itch grass, sorghum, goose grass, and barnyard grass was 36.1 ± 20.0, 42.7 ± 16.6, 28.3 ± 11.9, 7.6 ± 6.4, 8.1 ± 7.3 and 7.3 ± 6.6 days, respectively. Ovi- position rarely occurred on sorghum, goose grass and barnyard grass.
  • 0118Otero[A.-P. Liang]

    0118Otero[A.-P. Liang]

    Zootaxa 0000 (0): 000–000 ISSN 1175-5326 (print edition) https://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2019 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.0000.0.0 http://zoobank.org/urn:lsid:zoobank.org:pub:00000000-0000-0000-0000-00000000000 A New Species of Abbrosoga (Hemiptera: Fulgoroidea: Delphacidae), An Endemic Puerto Rican Planthopper Genus, with an Updated Checklist of the Delphacidae of Puerto Rico MIRIEL OTERO1,3 & CHARLES R. BARTLETT2 1Department of Plant Sciences & Plant Pathology, Montana State University, 10 Marsh Laboratories, 1911 W. Lincoln St. Bozeman MT, 59717, USA. E-mail: [email protected] 2Department of Entomology and Wildlife Ecology, 250 Townsend Hall, 531 S. College Ave., University of Delaware, Newark, Dela- ware, 19716-2160, USA. E-mail: [email protected] 3Corresponding author Abstract The genus Abbrosoga Caldwell (Delphacidae: Delphacinae: Delphacini) was described in Caldwell & Martorell (1951) to include the single species Abbrosoga errata Caldwell, 1951. Here, a second species, Abbrosoga multispinosa n. sp. is described. Revised diagnostics are presented for the genus and A. errata, including a key to species. A compiled list of 64 delphacid species from Puerto Rico is presented, with updated nomenclature, including the new species and a new record of Delphacodes aterrima for Puerto Rico. Key words: Delphacidae, Fulgoroidea, planthopper, new species, Abbrosoga, Puerto Rico Introduction The planthoppers (Hemiptera: Auchenorrhyncha: Fulgoroidea) encompass many species of economic importance, including important plant pathogen vectors (e.g., O’Brien & Wilson 1985, Wilson 2005). The Delphacidae are the second largest family of planthoppers (after Cixiidae) with more than 2,200 described species (Bartlett & Kunz 2015, Bourgoin 2018).
  • Journal of Virological Methods 270 (2019) 153–162

    Journal of Virological Methods 270 (2019) 153–162

    Journal of Virological Methods 270 (2019) 153–162 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet Delivery of maize mosaic virus to planthopper vectors by microinjection increases infection efficiency and facilitates functional genomics T experiments in the vector ⁎ Jianxiu Yaoa,1, Dorith Rotenbergb, Anna E. Whitfieldb, a Department of Plant Pathology, 4024 Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506, United States b Department of Entomology and Plant Pathology, 840 Main Campus Drive, North Carolina State University, Raleigh, NC 27606, United States ARTICLE INFO ABSTRACT Keywords: The corn planthopper, Peregrinus maidis, not only causes direct damage to plants by feeding, but also transmits Peregrinus maidis maize mosaic virus (MMV) to the plant hosts. The virus is transmitted in a propagative manner but the acqui- Maize mosaic nucleorhabdovirus sition of MMV by the vector feeding on infected plants can result in low acquisition and inoculation efficiency. Microinjection Here, we increased the acquisition efficiency by delivering the virus directly into the hemocoel through mi- PI3Kδ subunit croinjection, which resulted in efficient virus infection of the insect and transmission to maize. We found that RNAi delivery of virus by injection of 10 ng MMV (50 nl, 200 μg/ml virions) into P. maidis resulted in 93% transmission efficiency. In dose-response experiments, MMV abundance in insects and transmission efficiency decreased as the amount of virus inoculum delivered into the hemocoel was reduced. Examination of virus distribution in the vector using immunolabeling and confocal microscopy revealed similar tissue distributions in the injected in- sects when compared to those of previous studies using feeding on plants for virus acquisition.
  • Microinjection of Corn Planthopper, Peregrinus Maidis, Embryos for CRISPR/Cas9 Genome Editing

    Microinjection of Corn Planthopper, Peregrinus Maidis, Embryos for CRISPR/Cas9 Genome Editing

    Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing William Klobasa*,1, Fu-Chyun Chu*,1, Ordom Huot1, Nathaniel Grubbs1, Dorith Rotenberg1, Anna E. Whitfield1, Marcé D. 1 Lorenzen 1 Department of Entomology and Plant Pathology, North Carolina State University * These authors contributed equally Corresponding Author Abstract Marcé D. Lorenzen [email protected] The corn planthopper, Peregrinus maidis, is a pest of maize and a vector of several maize viruses. Previously published methods describe the triggering of RNA Citation interference (RNAi) in P. maidis through microinjection of double-stranded RNAs Klobasa, W., Chu, F.C., (dsRNAs) into nymphs and adults. Despite the power of RNAi, phenotypes generated Huot, O., Grubbs, N., Rotenberg, D., Whitfield, A.E., via this technique are transient and lack long-term Mendelian inheritance. Therefore, Lorenzen, M.D. Microinjection of the P. maidis toolbox needs to be expanded to include functional genomic tools that Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome would enable the production of stable mutant strains, opening the door for researchers Editing. J. Vis. Exp. (169), e62417, to bring new control methods to bear on this economically important pest. However, doi:10.3791/62417 (2021). unlike the dsRNAs used for RNAi, the components used in CRISPR/Cas9-based Date Published genome editing and germline transformation do not easily cross cell membranes. As March 26, 2021 a result, plasmid DNAs, RNAs, and/or proteins must be microinjected into embryos before the embryo cellularizes, making the timing of injection a critical factor for DOI success. To that end, an agarose-based egg-lay method was developed to allow 10.3791/62417 embryos to be harvested from P.
  • The Corn Delphacid Peregrinus Maidis(Ashmead

    The Corn Delphacid Peregrinus Maidis(Ashmead

    NationalNational PestPest AlertAlert The corn delphacid Peregrinus maidis (Ashmead, 1890) (Hemiptera: Fulgoroidea: Delphacidae) The corn delphacid is a widespread species also California and Hawaii. This species is that is most important in tropical regions also found throughout central and southern on corn and maize. In the U.S., this species Africa (from South Africa north to Senegal is most abundant in the southeast. In the and Sudan), Madagascar, Australia, Indone- central and mid-Atlantic states, Peregrinus sia, Malaysia, India, Sri Lanka, Philippines, maidis is not often reported, but probably Taiwan, Southern China, Vietnam and occurs through the northward movement many islands. of southern populations during the growing season. This species also may be important Description on sorghum and a number of grasses have been reported as alternate hosts includ- Like all delphacid planthoppers, this species ing itch grass (Rottboellia cochinchinensis has a large spur at the tip of the hind tibiae. Short-winged adults (Lour.) W.D. Clayton), goose grass (Eleusine Among delphacids, Peregrinus maidis is easy feeding on corn indica (L.) Gaertn.), gama grass (Tripsacum to recognize. It may occur as both long- seedling. dactyloides (L.) L.), and barnyard grass winged (macropterous) and short-winged (Echinochloa crus-galli (L.) P. Beauv.). Pereg- (brachypterous) forms. It is a relatively large rinus maidis is known to deposit eggs in non-host species. Pereg- species (long winged specimens exceeding 3 rinus maidis is a vector of several corn pathogens including Maize mm), with wings slightly patterned near the mosaic virus (Nucleorhabdovirus), Maize stripe virus (Tenuivirus), tip. The color is mostly brown, but con- “Maize line virus” and Maize Mal de Río Cuarto fijivirus.
  • The Flora and Fauna of Scottsdale's Mcdowell Sonoran Preserve

    The Flora and Fauna of Scottsdale's Mcdowell Sonoran Preserve

    The Flora and Fauna of Scottsdale’s McDowell Sonoran Preserve McDowell Sonoran Conservancy In partnership with the City of Scottsdale People Preserving Nature The flora and fauna survey of Scottsdale’s McDowell Sonoran Preserve was made possible by generous grants from the Nina Mason Pulliam Charitable Trust, the Arizona Game and Fish Department Heritage Fund, and an anonymous donor. Cover photo by: Ed Mertz The findings, opinions, and recommendations in this report are those of the investigators who have received partial or full funding from the Arizona Game and Fish Department Heritage Fund. The findings, opinions, and recommendations do not necessarily reflect those of the Arizona Game and Fish Commission or the Department, or necessarily represent official Department policy or management practice. For further information, please contact the Arizona Game and Fish Department. The Flora and Fauna of Scottsdale’s McDowell Sonoran Preserve A Project of the McDowell Sonoran Field Institute THE MCDOWELL SONORAN CONSERVANCY The McDowell Sonoran Conservancy champions the sustainability of the McDowell Sonoran Preserve for the benefit of this and future generations. As stewards, we connect the community to the Preserve through education, research, advocacy, partnerships and safe, respectful access. THE MCDOWELL SONORAN FIELD INSTITUTE The McDowell Sonoran Field Institute is the research center of the McDowell Sonoran Conservancy. Our mission is to study the environment of the McDowell Sonoran Preserve as well as the human history and human impacts on the Preserve. We do this by partner- ing with scientists and actively involving volunteers in research as citizen scientists. We use research results for long-term resource management, education, and to contribute to the broader scientific knowledge of natural areas.