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Fervidibacteria bacterium JGI 0000001-G10 contamination screened GBS-A 001 117 2264955041

Chthonomonas calidirosea T49 DSM 23976 Chthonomonas calirisosea T49 DSM 23976 2525259719

Candidatus Protochlamydia amoebophila UWE25 637501474 Symbiobacterium thermophilum IAM 14863 re-annotation 2608094028

Sulfobacillus thermosulfidooxidans AT-1 DSM 9293 2506241430

Buchnera aphidicola Sg re-annotation 2607624300

Marinithermus hydrothermalisOceanithermus T1 profundus DSM 14884 506 DSM 2504660101 14977 649789985

Deinococcus radiodurans ATCC BAA-816 637035817 Desulfovibrio magneticus RS-1.1083 644801580 Helicobacter hepaticus 3B1 ATCC 51449 re-annotation 2607801692

Deinococcus misasensis DSM 22328 2571067671

Meiothermus ruber 21 DSM 1279 646673331

Candidatus Blochmannia vafer BVAF 649854767

Rhodopseudomonas palustris BisA53 re-annotation.1123 2607484322

Campylobacter coli CVM N29710 2556024408 ureolyticus ACS-301-V-Sch3b 2559080506 Thermoflexus hugenholtzii JAD2 Draft Genome.1115 2143740193 Truepera radiovictrix RQ-24 DSM 17093 646858387 Candidatus Blochmannia chromaiodes 640 2521990876

Arcobacter nitrofigilis DSM 7299.1367 646815191 Leptonema illini 3055 DSM 21528 2506860732

Helicobacter acinonychis Sheeba 638057548

Hydrogenobaculum sp. Y04AAS1.1298 642750555 Fimbriimonas ginsengisoli Gsoil 348 2559125768

Oceanithermus profundus 506 DSM 14977.1857 649789944 Baumannia cicadellinicola Hc 637981721 Thermoanaerobaculum aquaticum MP-01 2579978622 Candidatus Portiera aleyrodidarum TV 2518564957 Chloroflexus aurantiacus J-10-fl.1419 641373589 Thermovibrio ammonificans HB-1 DSM 15698.1111 649882222 Herpetosiphon aurantiacus DSM 785 2509062064 Gluconobacter oxydans WSH-003 2549816083 Holophaga foetida TMBS4 DSM 6591.1344 2509787342 Kozakia baliensis SR-745 2584326144

Conexibacter woesei ID131577 DSM 14684 646501778 aeolicus VF5.1635 637020344 Nitratiruptor sp. SB155-2 640819569 Caldilinea aerophila STL-6-01 DSM 14535 2513223202 Psychrobacter sp. PRwf-1 640599912 SM-1 651043877 Gluconacetobacter diazotrophicus PAl 5 DSM 5601.1512 641336412 100

gamma proteobacterium sp. HdN1.1886 2511660077 Tolumonas auensis TA 4 DSM 9187 643874135

Anaerolinea thermophila UNI-1 649904556 Geobacter bemidjiensis Bem DSM 16622.1662 642766132 Weeksella virosa 9751 DSM 16922 650482497 Acinetobacter oleivorans DR1 648060746 Hydrogenobaculum sp. Y04AAS1 642750564 Roseiflexus sp. RS-1 640595831 Chromohalobacter salexigens 1H11 DSM 3043 637968334 Rubritalea marina DSM 17716 SAORIC-165 2590799310

Pelobacter carbinolicus DSM 2380 re-annotation 2607640403 Solitalea canadensis USAM 9D DSM 3403.789 2509579665 gamma proteobacterium sp. HdN1 2511660828

Candidatus Entotheonella sp. TSY2 2563963372 Dyadobacter tibetensis Y620-1.999 2586753254

Nitrosococcus halophilus Nc4 646692716 Geobacter uraniireducens Rf4 640547816 Chitinophaga pinensis UQM 2034Sulfurihydrogenibium DSM 2588 644969139 sp. YO3AOP1 642657166 Geopsychrobacterelectrodiphilus DSM 16401 2522770125 Leadbetterella byssophila 4M15 DSM 17132.820 649732680 Crenotaleadelta proteobacteriumthermophila DSM NaphS2.1681 14807 2601542956 648669972 58 Fluviicola taffensis RW262 DSM 16823 2503736480 Methylococcus capsulatus Bath 637170525 Dysgonomonas capnocytophagoides DSM 22835 2526215975 Acidiphilium cryptum JF-5.714 640553022 abyssi LF13 DSM 13497.965 2562177339 Candidatus Kuenenia stuttgartiensis.1850Gillisia sp. Hel 642555643I 86.1337 2595331630 bacterium JKG1 2541000616 Chitinophaga pinensis UQM 2034 DSM 2588.1582 644965986 Sporocytophaga myxococcoides DSM 11118 2541027769 Calescamantes bacterium SCGC AAA471-M6 contaminationFlavobacterium screened johnsoniae GBS-N 001 UW101 25 2264958556ATCC 17061 644740635Aquifex aeolicus VF5 637020292

Sulfobacillus acidophilus NAL DSM 10332.1443 2506614835 90 xylanophilus DSM 9941 638036424 100 Flavobacteriaceae bacterium 3519-10 644939626 bacterium FNE-F8 bin 25 2 bact.1467 2596885263

Cytophaga aurantiaca DSM 3654 2515812420 Arcobacter nitrofigilis DSM 7299 646814882 Fibrobacter succinogenes succinogenes S85 ATCC 19169.1160 646370460 Sulfurospirillum arcachonense DSM 9755.1352 2524848317 Spirosoma linguale DSM 74 646499502 100 Desulfobulbus propionicus 1pr3 DSM 2032 649926896 57 Pedobacter saltans Steyn 113 DSM 12145.1327 649983864 96 maritima MSB8 637006502 Desulfurispirillum indicum S5 DSM 22839 649843175 Haliscomenobacter hydrossis O DSM 1100 2504769684 98

Metallosphaera sedula DSM 5348 640507790 Alistipes sp. ZOR0009 2527112994 54 85 94 Gemmatimonas aurantiaca T-27T.1077 643774244 Ehrlichia canis Jake re-annotation 2607010834 Candidatus Solibacter usitatus Ellin6076.1351 639679667 AP bacterium SCGC AAA003-K101348010218044 2524119389 80 AnaeromyxobacterAcidimicrobium sp. Fw109-5 640845681 ferrooxidans ICP DSM 10331 644951524 Sulfolobus acidocaldarius N8 2524415498 71 Bdellovibrio bacteriovorus Tiberius 2519471229 65 Candidatus Koribacter versatilis Ellin345 637983312 Thermococcus kodakarensis KOD1 re-annotation 2607314188 Gemmatimonadetes bacterium KBS708 2578408886 Sulfolobales archaeon Acd1 2525231993 sp. SAG7 2509791783 99 100 Sorangium cellulosum So0157-2.563 2563706290 Propionibacterium acnes 266 651118367 Thermofilum pendens Hrk 5 639773865 59 Haliangium ochraceum SMP-2 DSM 14365 646386635 AP ActinobacteriaAP actinobacterium bacterium SCGC SCGC AC-312-N201351631988939.1794 AAA028-N15 1336627476846 2524116074 2524338249 Ignicoccus hospitalis KIN4/I DSM 18386 640897722 62 Candidatus Chloracidobacterium thermophilum B.1595 2512359033 CandidatusAcidithrix Microthrix ferrooxidans parvicella Py-F3Jonesia Bio17-1 AFO 2567188664denitrificans 2587990457Atopobium 55134 sp. ICM58DSM 20603 2538572489 645003284 100 Ilumatobacter coccineumPropionibacterium YM16-304.1284 2546182444 freudenreichii shermanii CIRM-BIA1 649640264 haemolyticum CCM DSM 20595 646848202 100 Nitriliruptor alkaliphilus DSM 45188 2513186726 Candidatus Korarchaeum cryptofilum OPF8 641613230 curtisii ATCC 43063 648047695 Thermofilum sp. 2505620323 99 Streptomyces coelicolor A32.1639 637268846 94 96 97 Leptospira biflexa sv. Patoc 1 Ames 642686283 Acidilobus saccharovorans 345-15 648117285 95 Crenarchaeota archaeon SCGC AAA471-O08 contamination screened GBS-N 001 29 2264867704 Aeropyrum camini SY1 JCM 12091 2556043927 56 Leptospira borgpetersenii sv. Hardjo-bovis L550 639656974 99 64 66 100 70 62 68 Fervidicoccus fontis Kam940 2514086899 77 94 55zeta proteobacterium SCGC AB-133-G06.1825 2264882311 Thaumarchaeota archaeon DS1 Dragon Thaumarchaeon 2263082816 100 IgnavibacteriumChloroherpeton album thalassium Mat9-16 ATCC JCM 1651135110.669 2514223700 642718389 Turneriella parva H DSM 21527 2506605018 64 Rhodothermus marinus R-10 DSM 4252 646410537 Thermodesulforhabdus norvegica DSM 9990 2599302638 54 Aminicenantes bacterium JGI 0000014-B08 contamination screened Etoliko 001 226 2265038112 Pyrobaculum islandicum DSM 4184.515 639783402 Pyrolobus fumarii 1A DSM 11204 2505690300 97 Geoarchaeota archaeon OSPB-1 8 best scaffolds 2504813064 99 NitrospinaKtedonobacter sp. SCGC racemifer AAA288-L16 SOSP1-21 2264263600 DSM 44963.1882 648605684 Hyperthermus butylicus DSM 5456 640081800 Desulfovermiculus halophilus DSM 18834 2558084374 HippeaThermobaculum maritima MH2 terrenum DSM 10411 YNP1 2504136247 ATCC BAA-798 646440071 Pyrobaculum arsenaticum PZ6 DSM 13514.983 640469832 100 Nitrospina sp. SCGC AAA288-L16.1695 2264263095 72 ThermomicrobiumPhycisphaera roseum DSM mikurensis 5159 643625174 NBRC 102666 2562259987 63 Geobacter uraniireducens Rf4.1416 640551709 Thermoproteus uzoniensis 768-20.892 650771298 GemmataPlanctomyces obscuriglobus limnophilus UQM 2246 Mu 290642222978 DSM 3776.733 646789588 86 Pirellula staleyi DSM 6068.602 646478078 66 SingulisphaeraIsosphaera acidiphila pallida MOB10 IS1B DSM ATCC 18658.1485 43644 649911973 2509619317 Pyrobaculum islandicum DSM 4184 639784710 67 84 63 100 Desulfovibrio desulfuricans desulfuricans ATCC 27774 643582468 Thermoproteus uzoniensis 768-20 650772475 72 86 65 94 Thermodesulfatator indicus CIR29812 DSM 15286 2505284957 73 Candidatus MagnetobacteriumAzoarcus toluclasticus sp. MYR-1 ATCC 2586913386 700605.781 2518923217 Ferroglobus placidus AEDII12DO DSM 10642 646612834 100 90 Candidatus defluvii.1895 649658772 55 Candidatus Entotheonella sp. TSY2.1885 2563963368 Candidatus Chloracidobacterium thermophilum B 2512360915 Archaeoglobus profundus Av18 DSM 5631 646510283 Candidatus Koribacter versatilis Ellin345.1275 637983305 65 99 Candidatus Solibacter usitatus Ellin6076 639685213 Archaeoglobus veneficus SNP6 DSM 11195 2504145093 60 98 85 88 Acidobacteriaceae bacterium KBS 96 DSM 24297.1530 2522227550 89 93 Bryobacter aggregatus DSM 18758.1413 2562803776 Caldivirga maquilingensis IC-167 641275785 100 Holophaga foetida TMBS4 DSM 6591 2509787193 Singulisphaera acidiphila MOB10 DSM 18658 2509622579 Vulcanisaeta distributa DSM 14429 648202454 89 bacterium SCGC AAA164-E04.1390 2236430667 100 Acidobacterium capsulatum ATCC 51196.1685 643768772 Geodermatophilus obscurus G-20 DSM 43160 646517372 73 98 70 Opitutaceae sp. TAV1 2508839915 Streptomyces griseus griseus NBRC 13350 641695087 100 ThermodesulfovibrioAcidobacteriaceae yellowstonii DSM 11347.23 bacterium 643375170 URHE0068.810 2556998364 Micromonospora sp. L5.1353 649814389 Thermodesulfovibrio yellowstonii DSM 11347 643375359 1 Thermodesulfobacterium thermophilum DSM 1276.1498 2523967454 Thermodesulfobium narugense Na82 DSM 14796 2504785159 Streptosporangium roseum NI 9100 DSM 43021.931 646455177 Fulvimarina pelagi HTCC2506 639869151 77 76 66 100 Thermodesulfobacterium geofontis OPF15 2506601810 Picrophilus torridus DSM 9790 re-annotation 2608116003 100 Leptospirillum ferriphilum80 ML-04 2519478366 Pelobacter propionicus DSM 2379 639754679 Thiorhodospira sibirica A12 ATCC 700588 2509461158 Thermoplasma volcaniumThaumarchaeota GSS1 re-annotation archaeon 2607367955 BS1 2503002316 Magnetococcus sp. MC-1 639724371 Thioalkalivibrio Gemmatimonasthiocyanoxidans aurantiacaARh 4 2506727261 T-27T 643775007 51 Mariprofundus ferrooxydans PV-1 639880934 Lebetimonas sp. JS085 2516462183 70 72 Acidiphilium cryptum JF-5 640552853 100 100 Thiovulum sp. ES.1572 2532209046Thermovibrio ammonificans HB-1 DSM 15698 649882977 87 RoseobacterGluconacetobacter denitrificans diazotrophicus OCh 114 639636604 PAl 5 DSM 5601 641336978 Methanoregula formicica SMSP DSM 22288 2509037109 94 65 59 Candidatus Odyssella thessalonicensis L13 2548245798 Hirschia baltica ATCC 49814 644903686 Rickettsiales bacterium Ac37b 2599225900 68 67 86 Phenylobacterium zucineum HLK1 642759693 Salinispora tropica CNB-440 640472677 Meganema perideroedes DSM 15528 2516051696 Kribbella flavidaStreptomyces IFO 14399Nitriliruptor DSM coelicolor 17836 alkaliphilus A32646492762 637268879 DSM 45188.1149 2513186711 Dermacoccus Intrasporangiumnishinomiyaensis calvum M25 2588617028 7KIP DSM 43043 649828776 94 Paenibacillus sp. JDR-2 644862785 Candidatus Microthrix parvicellaDesmospora Bio17-1.450 sp. 8437 2567188683 651607907 AP bacterium SCGC AC-312-N201351631988939 2524116063 Ehrlichia canis Jake re-annotation.1651 2607010198 Paenibacillus mucilaginosus KNP414 650954037 53 Candidatus Liberibacter americanus Sao Paulo 2555882208 50 60 endosymbiont of AcanthamoebaWolbachia sp. endosymbiont UWC8 2586111537 of Drosophila melanogaster re-annotation 2607412186 68 83 Anaplasma centrale Israel 646445382 69 99 98 66 56 Shimazuella kribbensis DSM 45090 2515799536 alpha proteobacterium sp. HIMB59 2504110930 Kyrpidia tusciae T2Brevibacillus DSMTumebacillus 2912 brevis 646751655 flagellatus NBRC 100599 GST4 643788262 2587392152 96 84 Thioalkalimicrobium cyclicum ALM1 DSM 14477 2505699943 55 Candidatus Midichloria mitochondrii IricVA 650987075 Geobacillus thermodenitrificansBacillus NG80-2 selenitireducens 640194138 MLS10 646852850 70 99 Candidatus Xenolissoclinum pacificiensis L6 2579203963 Deferribacter desulfuricans SSM1 DSM 14783 646662670 Candidatus Ruthia magnifica Cm 639757922 Kyrpidia tusciae T2 DSM 2912.1854 646751961 Neorickettsia helminthoeca Oregon 2578361279 Candidatus Methylomirabilis oxyfera 254062513265 Azoarcus sp. BH72 639786550 94 Nitrosococcus watsoni C-113 648073144 56 Verminephrobacter eiseniae EF01-2 639850561RickettsiaceaeCandidatus bacterium Rickettsia Os18 amblyommii 2586252932 GAT-30V 2512817077

67 Coxiella burnetii Dugway 5J108-111 640888465Francisella philomiragia philomiragia ATCC 25017 641556356 Halobacterium salinarum R1 DSM 671 641566126

Pedosphaera parvula Ellin514 645133649 94 Orientia tsutsugamushi Boryong 640568326 Methylacidiphilum infernorum V4 642666452 Marine Group III euryarchaeote sp. SCGC AAA288-E19 2264261902 Legionella longbeachaesyncytium NSW150 symbiont 648036243 of Diaphorina citri 2566131878 Opitutus terrae PB90-1.402 641690095 58 Acidithiobacillus ferrooxidansCandidatus ATCC 53993 Kinetoplastibacterium 642790104 desouzaii TCC079E 2563631924 76 Kangiella koreensis SW-125 DSM 16069 644987895 84 96 60 Candidatus Kinetoplastibacterium oncopeltii TCC290E 2563632704 Methanomassiliicoccus luminyensis B10 2518907083 Candidatus Nitrospira defluvii 649658397 100 marine bacterium HIMB624 2503354445 Candidatus Zinderia insecticola CARI 648177497 Pelosinus sp. HCF1 2511471913

99 53 Neisseria gonorrhoeaeHalothiobacillus NCCP11945 neapolitanus 642722929 c2 ATCC 23641 646384332 Dehalococcoides mccartyi 195 637120497 Nostoc sp. PCC 7120 63723528762 Thermoplasmatauncultured archaeon 2502232557 marine group II euryarchaeoteRhodanobacter 2511795602Pseudoxanthomonas sp. 2APBS1 suwonensis 2506719705 11-1 649877649 Ktedonobacter racemifer SOSP1-21 DSM 44963 648604919 99 Alkalilimnicola ehrlichii MLHE-1 638126712 Verrucomicrobium spinosum DSM 4136 642334241 62 AP Oxalobacteraceae bacterium JGI 001003-B031337237172778 contamination screened 2561411616 75 Candidatus Omnitrophus fodinae SCGC AAA011-A17 contamination screened Dusel 001 263 2265030246 Syntrophothermus lipocalidus DSM 12680 646853290 93 MethanohalophilusMethanosaeta mahii thermophila SLP DSM PT 5219 639700078 646707643 59 70 Halorhodospira halophila SL1 639856221 Verrucomicrobia bacterium SCGC AAA164-M04 2236337506 Archaeoglobus sulfaticallidus PM70-1 DSM 19444 Asulf version1 2522261564 Anaerolinea thermophila UNI-1.1078 649904763 Nitrosopumilus maritimus SCM1 641315910 3 SelenomonasSelenomonas flueggei sputigena ATCC 43531 ATCC 64548237835185 646080630 60 Obscuribacter phosphatis Mle1 12 2557140141 Verrucomicrobia bacterium SCGC AAA300-N18 2236287310 Desulfurispirillum indicum S5 DSM 22839.770 649842914 Thermoflexus hugenholtzii JAD2 Draft Genome 2143742483 Akkermansia muciniphila ATCC BAA-835 642613664 54 56 52 Roseiflexus sp. RS-1.323 640596592 DesulfonisporaCarboxydothermus thiosulfatigenes hydrogenoformans GKNTAUT DSM Z-2901 11270 DSM 2501938926 6008 637754156 2597502528

96

78

97 Caldilinea aerophila STL-6-01 DSM 14535.982 2513223221 Euryarchaeota archaeon SCGC AB-637-J17 Draft2 2574390727

83 Zavarzinella formosa DSM 19928.1173 2550723494

Gemmata obscuriglobus UQM 2246.1196 642220818 95 663517635 Thaumarchaeota archaeon BS4 2519956012

Candidatus Omnitrophus fodinae SCGC AAA011-A17 contamination screened Dusel 001 263.1603 2265029268 Blastopirellula marina SH 106T DSM 3645 638975736

96 Fibrobacter succinogenes succinogenes S85 ATCC 19169 646370660 Maribacter sp.Bacteroides HTCC2170 648162150 fragilis NCTC 9343 637226519

Syntrophobacter fumaroxidans MPOB.1762 639700963 marianensis 7p75a DSM 12885 649832023 Symbiobacterium thermophilum IAM 14863 re-annotation.1000 2608095241

Planctomyces limnophilus Mu 290 DSM 3776 646785966

Niastella koreensis GR20-10 DSM 17620.433 2506623073

Syntrophorhabdus aromaticivorans UI 2509869892 Syntrophobacter fumaroxidans MPOB 639702703

Fulvivirga imtechensis AK7 2532713542

Flexithrix dorotheae DSM 6795 2515807441 98

Alistipes sp. ZOR0009.1637 2527112702 Chlorobium tepidum TLS 637115720 Spirosoma linguale DSM 74.1829 646500060

Desulfatirhabdium butyrativorans DSM 18734 2524231083 Sorangium cellulosum So0157-2 2563709958 Anaeromyxobacter sp. Fw109-5.947 640842790 Microscilla marina ATCC 23134 640217611

Armatimonadetes bacterium JGI 0000077-K19 contamination screened TAbiofilm 001 240 2265017457 Caldithrix abyssi LF13 DSM 13497 2562178885 Fervidibacteria bacterium JGI 0000001-G10 contaminationFlectobacillus screened major GBS-A VKMB-859 001 DSM 117.1851 Cesiribacter103.645 2264954932 2509857565 andamanensis AMV16 2531511262 bacterium SCGC AB-629-J13 2264925344

Bacteriovorax marinus SJ 2563366529

RunellaDyadobacter slithyformis LSU4alkalitolerans DSM 19594.1666 DSM 23607 2505791548 2524297745 Pontibacter actiniarum KMM 6156 DSM 19842 2502681828 Emticicia oligotrophica GPTSA100-15 DSM 17448 2506488949 Ignavibacterium album Mat9-16 JCM 16511.1221 2514223461

Arcticibacter svalbardensis MN12-7.689 2545494334 Deferrisoma camini S3R1 2517271504 Candidatus sp. WGA-4E final clean version 2265138133 2

Olivibacter sitiensis DSM 17696Solitalea 2515273230 canadensis USAM 9D DSM 3403.906 2509577180 Sporocytophaga myxococcoides DSM 11118.551 2541027364 1 Sphingobacterium sp. ML3W.1043 2598916429

Opitutus terrae PB90-1 641690379

Desulfobotulus alkaliphilusASO4-4 2597047456

Pseudosphingobacterium domesticum DSM 18733.1175 2599441785 DesulfurivibrioAHT2 646847145 alkaliphilus

Leadbetterella byssophila 4M15 DSM 17132 649733287 Pedobacter saltans Steyn 113 DSM 12145 649982845

Cytophaga aurantiaca DSM 3654.1631 2515812548

Desulfurobacterium sp. TC5-1 2523816273

Desulfovibrio magneticus RS-1 644803049 Thermonema rossianum DSM 10300 2523887628

Desulfobulbus alkaliphilusAPS1 2595071541

Marinimicrobia bacterium SCGC AAA003-L8 contamination screened Tropical gyre 001 269 2265101559 delta proteobacterium NaphS2 648670175 Desulforegula conservatrix Mb1Pa DSM 13527 2523439722 2 Desulfonatronospira thiodismutansASO3-1 644405185

Candidatus Amoebophilus asiaticus 5a2 642681508

Flexibacter litoralis Fx l1 DSM 6794 2509563440