Paldispot Factsheet

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Pan-Lyme Disease Strain Detection PaLDiSPOT Lyme disease referred to a disease caused by Borrelia, Babesia or Bartonella species, and affect different organs of human body (skin, heart, nervous system). B. burgdorderi sensu stricto, consist the strain with higher pathogenicity and transmitted by ticks, however Borrelia afzelii and Borrelia garinii also exhibit high pathogenicity. Apart from the previous genospecies, borreliosis caused also by Borrelia burgdorferi sensu lato complex (Borrelia lanei, Borrelia californiensis, Borrelia bissettiae etc). Early Lyme disease characterized by erythema migrans fever, malaise, fatigue, headache, myalgias, and arthralgias that appear within thirty days post tick bite, while other skin lesions have also been recorded (lymphocytoma). Delay in treatment of early borreliosis might affect other organs (musculoskeletal system, central nervous system, heart) while late borreliosis includes serious diseases such as arthritis and encephalitis. Diagnosis can be performed observing clinical manifestations of Lyme disease, as well as with serological testing. The identification of Borellia genospieces through Polymerase Chain Reaction (PCR) is very sensitive and has the potential of detection at very low copies, even before activation of the immune system. PaLDiSPOT is used for detection and determination of different Borrelia, Babesia and Bartonella genospecies using sensitive molecular biology techniques. The use of positive and internal controls, as well as the unique design of the platform, ensures high sensitivity and specificity. PaLDiSPOT detects the presence of different species in blood or plasma, even at early stages of the disease. PaLDiSPOT enable detection of: Borrelia burgdorferi Borrelia spielmanii Borrelia afzelii Borrelia carolinensis Borrelia garinii Borrelia americana Borrelia mayonii Borrelia sinica Borrelia lusitaniae Borrelia yangtzensis Borrelia valaisiana Borrelia hermsii Borrelia kurtenbachii Bartonella henselae Borrelia lanei Bartonella bacciliformis Borrelia californiensis Bartonella vinsonii Borrelia bissettiae Babesia microti Borrelia bavariensis Babesia duncnani Borrelia turcica .

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Complement Evasion Contributes to Lyme Borreliae-Host Associations
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/341602682 Complement Evasion Contributes to Lyme Borreliae–Host Associations Article in Trends in Parasitology · May 2020 DOI: 10.1016/j.pt.2020.04.011 CITATIONS READS 0 37 4 authors, including: Maria Diuk-Wasser Peter Kraiczy Columbia University Goethe-Universität Frankfurt am Main 159 PUBLICATIONS 3,159 CITATIONS 216 PUBLICATIONS 4,537 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Geographic expansion and landscape determinants of tick-borne disease View project Epidemiology of Lyme disease in the Northeast US View project All content following this page was uploaded by Maria Diuk-Wasser on 18 July 2020. The user has requested enhancement of the downloaded file. Trends in Parasitology Review Complement Evasion Contributes to Lyme Borreliae–Host Associations Yi-Pin Lin,1,2,* Maria A. Diuk-Wasser,3 Brian Stevenson,4,5 and Peter Kraiczy6,* Lyme disease is the most common vector-borne disease in the northern hemi- Highlights sphere and is caused by spirochetes of the Borrelia burgdorferi sensu lato complex. Emerging and re-emerging zoonotic dis- Lyme borreliae infect diverse vertebrate reservoirs without triggering apparent eases have a major impact on global manifestations in these animals; however, Lyme borreliae strains differ in their res- public health, including tick-transmitted illnesses such as Lyme disease. Lyme ervoir hosts. The mechanisms that drive those differences are unknown. To survive disease-causing pathogens develop a in vertebrate hosts, Lyme borreliae require the ability to escape from host defense range of sophisticated strategies to over- mechanisms, in particular complement.
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Isolation of the Lyme Disease Spirochete Borrelia Mayonii from Naturally Infected Rodents in Minnesota
HHS Public Access Author manuscript Author ManuscriptAuthor Manuscript Author J Med Entomol Manuscript Author . Author Manuscript Author manuscript; available in PMC 2018 July 01. Published in final edited form as: J Med Entomol. 2017 July 01; 54(4): 1088–1092. doi:10.1093/jme/tjx062. Isolation of the Lyme Disease Spirochete Borrelia mayonii From Naturally Infected Rodents in Minnesota Tammi L. Johnson1, Christine B. Graham, Andrias Hojgaard, Nicole E. Breuner, Sarah E. Maes, Karen A. Boegler, Adam J. Replogle, Luke C. Kingry, Jeannine M. Petersen, Lars Eisen, and Rebecca J. Eisen Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 3156 Rampart Rd., Fort Collins, CO 80521 Abstract Borrelia mayonii is a newly described member of the Borrelia burgdorferi sensu lato complex that is vectored by the black-legged tick (Ixodes scapularis Say) and a cause of Lyme disease in Minnesota and Wisconsin. Vertebrate reservoir hosts involved in the enzootic maintenance of B. mayonii have not yet been identified. Here, we describe the first isolation of B. mayonii from naturally infected white-footed mice (Peromyscus leucopus Rafinesque) and an American red squirrel (Tamiasciurus hudsonicus Erxleben) from Minnesota, thus implicating these species as potential reservoir hosts for this newly described spirochete. Keywords Borrelia mayonii; Ixodes scapularis; Peromyscus leucopus; Tamiasciurus hudsonicus; Lyme disease The recently described Lyme disease spirochete, Borrelia mayonii, causes human disease in Minnesota and Wisconsin, and it has been detected in naturally infected Ixodes scapularis Say ticks collected in both states (Pritt et al. 2016a, b). Laboratory studies have confirmed that I.
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Lipid Hijacking: a Unifying Theme in Vector-Borne Diseases Anya J O’Neal1*, L Rainer Butler1, Agustin Rolandelli1, Stacey D Gilk2, Joao HF Pedra1*
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A Murine Model of Lyme Disease Demonstrates That Borrelia Burgdorferi Colonizes the Dura Mater And
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.24.060681; this version posted April 25, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 A Murine Model of Lyme Disease Demonstrates That Borrelia burgdorferi Colonizes the Dura Mater and 2 Induces Inflammation in the Central Nervous System. 3 4 Timothy Casselli1*, Ali Divan1ϕ, Yvonne Tourand1, Heidi L. Pecoraro2, and Catherine A. Brissette1*. 5 6 1Department of Biomedical Sciences, University of North Dakota, School of Medicine and Health Sciences, 7 Grand Forks ND, USA. 8 9 2Veterinary Diagnostic Services Laboratory, North Dakota State University, Fargo ND, USA. 10 11 Running title: Borrelia burgdorferi induces CNS inflammation in mice. 12 13 *Corresponding authors: 14 Catherine A. Brissette. Department of Biomedical Sciences, University of North Dakota, School of Medicine 15 and Health Sciences, Grand Forks, ND 58202-9061, USA. 16 Email: [email protected]; Tel: 1-701-777-6412. 17 Timothy Casselli. Department of Biomedical Sciences, University of North Dakota, School of Medicine and 18 Health Sciences, Grand Forks, ND 58202-9061, USA. 19 Email: [email protected]; Tel: 1-701-777-6414. 20 21 22 ϕCurrent address: BioLegend, 8999 Biolegend Way, San Diego, CA 92121. 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.04.24.060681; this version posted April 25, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
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Borrelia Mayonii Fact Sheet
Borrelia mayonii Disease What is Borrelia mayonii person may not know they have been bitten disease? by a tick. Borrelia mayonii disease is one of many tickborne diseases in Minnesota. The What are the symptoms of disease agent is closely related to the Borrelia mayonii disease? bacteria that cause Lyme disease, Borrelia The spectrum of illness is still being burgdorferi. It was first identified as a cause described for this new, rare disease. of human illness in a Minnesota resident in However, the most common symptoms 2013. Since then, low numbers of cases reported to date include: have been reported in persons with exposure to ticks only in the upper ▪ Fever or chills Midwest. ▪ Muscle or joint pain ▪ Headache ▪ Rash How do people get Borrelia mayonii disease? Similar to Lyme disease caused by B. burgdorferi, People can get B. mayonii disease most arthritis (joint inflammation likely through the bite of a blacklegged tick and swelling) has also been reported as a (deer tick) that is infected with B. mayonii later stage of infection in patients with B. mayonii. bacteria. Not all blacklegged ticks carry illnesses due to these bacteria and not all people bitten by a blacklegged tick will get sick. Similar to B. How is Borrelia mayonii burgdorferi, the tick must be attached to a disease diagnosed? person for at least 24-48 hours before it can spread B. mayonii bacteria. If a person suspects B. mayonii disease, they should contact a doctor as soon as Blacklegged ticks live on the ground in areas possible for diagnosis and treatment.
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No Evidence of Borrelia Mayonii in an Endemic Area for Lyme Borreliosis in France Pierre H
Boyer et al. Parasites & Vectors (2017) 10:282 DOI 10.1186/s13071-017-2212-7 SHORTREPORT Open Access No evidence of Borrelia mayonii in an endemic area for Lyme borreliosis in France Pierre H. Boyer1, Sylvie J. De Martino1,2, Yves Hansmann3, Laurence Zilliox2, Nathalie Boulanger1,2 and Benoît Jaulhac1,2* Abstract Background: Borrelia mayonii is currently the latest species belonging to the Borrelia burgdorferi (sensu lato) complex to be discovered. Interestingly it is involved in human pathology causing a high fever. We looked for itspresenceinpost-tickbitefebrilepatientsaswellasinIxodes ricinus ticks in an endemic area of France. Results: After ensuring that our molecular technics correctly detected B. mayonii, 575 patients and 3,122 Ixodes ricinus nymphs were tested. Neither B. mayonii nor another species of the B. burgdorferi (s.l.) complex previously not reported in Europe has been identified. Conclusions: For now, B. mayonii seems to be an epiphenomenon. However, its discovery broadens the etiology of post-Ixodes bite febrile syndromes. Keywords: Lyme borreliosis, Borrelia mayonii, Borrelia burgdorferi (sensu lato), Fever Background Borrelia, thus expanding the field of symptoms found in Lyme borreliosis is reported as the most common tick- Lyme borreliosis. We, therefore, investigated its potential borne disease in the United States [1] and all the northern presence in patients presenting with fever after a suspicion hemisphere. Since the early research of Baranton and col- of tick bite in Alsace, a French region endemic for Lyme leagues [2], several species belonging to the Borrelia burg- borreliosis [5], and also in Ixodes ricinus,thevectorofB. dorferi (sensu lato)(s.l.) complex have been described.
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Lyme Disease PCR, Spinal Or Synovial Fluid
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Primordial Origin and Diversification of Plasmids in Lyme Disease Agent Bacteria Sherwood R
Casjens et al. BMC Genomics (2018) 19:218 https://doi.org/10.1186/s12864-018-4597-x RESEARCH ARTICLE Open Access Primordial origin and diversification of plasmids in Lyme disease agent bacteria Sherwood R. Casjens1,2,8*, Lia Di4, Saymon Akther3, Emmanuel F. Mongodin5, Benjamin J. Luft6, Steven E. Schutzer7, Claire M. Fraser5 and Wei-Gang Qiu3,4,9* Abstract Background: With approximately one-third of their genomes consisting of linear and circular plasmids, the Lyme disease agent cluster of species has the most complex genomes among known bacteria. We report here a comparative analysis of plasmids in eleven Borreliella (also known as Borrelia burgdorferi sensu lato) species. Results: We sequenced the complete genomes of two B. afzelii, two B. garinii, and individual B. spielmanii, B. bissettiae, B. valaisiana and B. finlandensis isolates. These individual isolates carry between seven and sixteen plasmids, and together harbor 99 plasmids. We report here a comparative analysis of these plasmids, along with 70 additional Borreliella plasmids available in the public sequence databases. We identify only one new putative plasmid compatibility type (the 30th) among these 169 plasmid sequences, suggesting that all or nearly all such types have now been discovered. We find that the linear plasmids in the non-B. burgdorferi species have undergone the same kinds of apparently random, chaotic rearrangements mediated by non-homologous recombination that we previously discovered in B. burgdorferi.These rearrangements occurred independently in the different species lineages, and they, along with an expanded chromosomal phylogeny reported here, allow the identification of several whole plasmid transfer events among these species.
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What Is Lyme Disease?
What is Lyme Disease? Lyme disease is caused by a spiral-shaped bacteria, Borrelia burgdorferi (Bb), or by newly discovered Borrelia mayonii. It is usually transmitted by the bite of an infected tick−Ixodes scapularis in the East, Ixodes pacificus in the West. How is Lyme disease transmitted? In the USA, the bacteria are transmitted to people and animals by the bite of an infected tick, Ixodes scapularis, (commonly called the blacklegged or deer tick), andIxodes pacificus (western blacklegged tick) in the West. Although other types of ticks such as the Dermancentor variabilis (American dog) and some insects have been shown to carry the Lyme bacteria, to date, transmission of Lyme through those vectors has not been proven. The longer a tick is attached, the greater risk of disease transmission. The Lyme bacteria has been proven to survive blood banking conditions; however, to date, no transmission has been proven through blood transfusions in humans. Studies have shown transmission through this route in mice in the lab. Note: Other tick-borne diseases have been transmitted through the blood supply. The bacteria can also be passed through the placenta of a pregnant woman to the fetus—congenital transmission. The DNA of the bacteria has been found in breast milk, but no transmission has been proven to date in humans. There is no proof to date that Lyme is sexually transmitted, although some preliminary studies have found PCR positives for the DNA of the Lyme bacteria in semen and in cervical tissue. These findings do not prove sexual transmission, but some physicians feel because the Lyme and syphilis spirochete (bacteria) are similar, Lyme may be sexually transmitted.
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