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Apoptosis Product List

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Tools for assessing cell events Apoptosis, cell cycle and cell proliferation Product list The balance of cell proliferation and apoptosis is important In addition to flow cytometry products, BD Life Sciences carries for both development and normal tissue homeostasis. Cell a broad portfolio of reagents for determination and detection proliferation is an increase in the number of cells as a result of of apoptotic and proliferative events by immunohistochemistry, growth and division. Cell proliferation is regulated by the cell cell imaging, and Western blot. cycle, which is divided into a series of phases. Apoptosis, or As part of our commitment to maximize scientific results, programmed cell death, results in controlled self-destruction. BD Life Sciences provides a variety of tools to assist customers Several methods have been developed to assess apoptosis, in their experimental setup and analysis. These include a cell cycle, and cell proliferation. BD Life Sciences offers a decision tree to guide in the selection of the most suitable complete portfolio of reagents and tools to allow exploration methods for a specific study. of the cellular features of these processes. BD Life Sciences carries high-quality reagents in the latest Over the years, multicolor flow cytometry has become essential formats to examine cell cycle, proliferation, and apoptosis in the study of apoptosis, cell cycle, and cell proliferation. across a variety of platforms, in applications from basic Success of the technology results from its ability to monitor research to drug screening. these processes along with other cellular events, such as protein phosphorylation or cytokine secretion, within heterogeneous cell populations. BD Life Sciences continues to innovate in this area with new products such as the BD Horizon™ cell proliferation dyes (VPD450 and CFSE), BD Horizon™ fixable viability stains, and popular reagents such as antibodies to cleaved PARP and caspase-3 available in new formats and for different types of applications. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Abbreviations The following are common abbreviations that might appear throughout the Apoptosis Reagents Product List. APPS Applications IF Immunofluorescence Ar Armenian hamster IHC Immunohistochemistry Bab Baboon IHC(F)* Immunohistochemistry—formalin (antigen retrieval required) BD IMag - DM Direct Magnet IHC(Fr) Immunohistochemistry—frozen Bioimg Bioimaging IHC(P) Immunohistochemistry—formalin fixed, paraffin Block Blocking IHC(Zn) Immunohistochemistry—zinc fixed Bov Bovine IP Immunoprecipitation Chick Chicken IP/WB Immunoprecipitation/Western blot Ctrl Controls ISH In situ hybridization Cost Co-stimulation Lym Lymphocyte Cyno Cynomolgus Mam Mammalian EA Enzyme assay Monk Monkey ELISA Enzyme-linked immunosorbent assay Ms Mouse ELISA Cap. ELISA Capture NA/LE No azide, low endotoxin ELISA Det. ELISA Detection Neu Neutralization ELISPOT Enzyme-linked immunospot NHP Non-human primate FA Functional assay PE Phycoerythrin FCM Flow cytometry Pg Pigeon FCM* Flow cytometry. Suitable for use with intracellular staining protocols using BD Cytofix/Cytoperm™. Pig Porcine FCM/IA Flow cytometry/immunoassay Prim Primate FITC Fluorescein isothiocyanate Qua Quail G Pig Guinea pig Rab Rabbit GMP Good Manufacturing Practices REACT Reactivity Gt Goat Rhe Rhesus Ham Hamster RUO For Research Use Only. Not for use in diagnostic or therapeutic procedures. HRP Horseradish peroxidase Sep Cell separation Hs Horse Syr Ham Syrian hamster Hu Human Tr Trout IC Intracellular staining Vir Viral ICtrl Isotype control WB Western blot IC Ctrl Intracellular control Xen Xenopus ICC Immunocytochemistry (cytospins) IC/FCM Intracellular staining (flow cytometry) Unless otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. 2 bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. Life, death and cell proliferation The balance of cell proliferation and apoptosis is important for both Several methods have been developed to assess apoptosis, cell cycle, development and normal tissue homeostasis. Cell proliferation is an and proliferation. BD Biosciences offers a complete portfolio of increase in the number of cells as a result of growth and division. Cell reagents and tools to allow exploration of the cellular features of these proliferation is regulated by the cell cycle, which is divided into a series processes. of phases. Apoptosis or programmed cell death results in controlled self- destruction. Cell biology decision tree Cell Biology Apoptosis Viability Proliferation and Cell Cycle Live Cells Fixed Cells Live Cells Fixed Cells Live Cells Fixed Cells Membrane Integrity Caspase Activity • Fixable Viability Stains Proliferation Dye Retention • Anti-active caspase-3 antibodies • Anti-cleaved PARP antibodies • VPD450* • CFSE Membrane Integrity DNA Fragmentation • Nucleic acid dyes DNA Content • APO-DIRECT (cell impermeable)† • Nucleic acid dyes • APO-BRDU (cell permeable)‡ Esterase Activity • Calcein AM Phosphatidylserine • Calcein Blue AM Proliferation Dye Retention • Annexin V • VPD450* • CFSE Caspase Activity DNA Content • Live Cell Caspase Probes • Nucleic acid dyes (all)†‡ Mitochondrial Membrane BrdU/EdU Incorporation Potential • BrdU Flow Kits • Mitoscreen (JC-1) • EdU Click Proliferation Kits • MitoStatus TMRE • Anti-BrdU antibodies • MitoStatus Red Histone H3 Phosphorylation • Anti-Histone H3 (pS28) *Violet Proliferation Dye 450 Ki-67 status †Cell permeable nucleic acid dyes include DRAQ5™, Hoechst 33342, and Hoechst 34580. ‡Cell impermeable nucleic acid dyes include DAPI, DRAQ7™, Propidium Iodide, Via-Probe (7-AAD), Via-Probe Green, and Via-Probe Red. • Anti–Ki-67 antibodies Unless otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. bdbiosciences.com 3 Kits/Sets/Pairs Description React Clone Isotype Apps Reg Format Size Cat. No. 488 EdU Click Proliferation Kit IC/FCM RUO EdU, 6-Fam Azide, 565455 Buffer Additive, Permeabilization and Wash Reagent, Fixative solution, Catalyst solution, DMSO 647 EdU Click Proliferation Kit IC/FCM RUO EdU, Eterneon™ Red 645 565456 Azide, Buffer Additive, Permaebilization and Wash Reagent, Fixative solution, Catalyst solution, DMSO Annexin V FITC Fluorescence Microscopy Kit IF RUO Annexin V-FITC, 25 Slides 550911 Annexin V Binding Buffer, and PBS Buffer Annexin V FITC Apoptosis Detection Kit I FCM RUO Annexin V-FITC, 100 tests 556547 Propidium Iodide Staining Solution, Annexin V Binding Buffer Annexin V FITC Apoptosis Detection Kit II FCM RUO Purified Recombinant 100 tests 556570 Annexin V, Annexin V-FITC, Propidium Iodide Staining Solution, Annexin V Binding Buffer Annexin V PE Apoptosis Detection Kit I FCM RUO Annexin V-PE, 7-AAD, 100 tests 559763 and Annexin V Binding Buffer Apoptosis Detection Kit (APO-BRDU) IC/FCM RUO FITC-Labeled Anti-BrdU60 tests 556405 mAb, PI/RNase Staining Buffer, Reaction Buffer, Rinsing Buffer, Wash Buffer, Br-dUTP, TdT Enzyme, Negative Control Cells, Positive Control Cells Apoptosis, DNA Damage, and Cell Proliferation Kit IC/FCM RUO BrdU PerCP-Cy™5.5, 50 tests 562253 H2AX (pS139) Alexa Fluor® 647, Cleaved PARP (Asp214) PE; DAPI; BrdU solution; DNase; BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution; BD Perm/Wash™ Buffer Apoptosis Detection Kit (APO-DIRECT) IC/FCM RUO FITC-dUTP, PI/RNase 50 tests 556381 Staining Buffer, Reaction Buffer, Rinsing Buffer, Wash Buffer, TdT Enzyme, Negative Control Cells, Positive Control Cells Apoptosis I Sampler Kit IF, WB RUO Apaf-1, Bad, Bax, Bcl-2, 10 μg each 612741 Bcl-x, BRUCE, CAS, hILP/XIAP, Nip1, p53 Apoptosis II Sampler Kit IF, WB RUO Caspase-2/ICH-1L, 10 μg each 612743 Caspase-3/CPP32, Caspase-7/MCH-3, DFF45, FADD, Fas/CD95/APO–1, PARP, RIP, TRADD Apoptotic Jurkat Lysate Set I WB RUO Jurkat Control Lysate 500 μg each 550959 and Camptothecin-treated Jurkat Lysate Bcl-2 Related Sampler Kit IF, WB RUO Bad, BAG-1, Bax, Bcl-2, 10 μg each 612742 Bcl-x, Bcl-x, Beclin, Bid BrdU APC Flow Kit IC/FCM RUO 50 tests 552598 IC/FCM RUO 4 x 50 tests 557892 BrdU FITC Flow Kit IC/FCM RUO 50 tests 559619 IC/FCM RUO 4 x 50 tests 557891 BrdU In-Situ Detection Kit IHC(Zn), IHC(F), IHC(Fr) RUO Fixation Buffer, Diluent 50 tests 550803 Buffer, BD™ Retrievagen A Antigen Retrieval Solution, biotinylated anti-BrdU antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) Solution, DAB Buffer, DAB Chromogen, and Control Slides Unless otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. 4 bdbiosciences.com All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. Kits/Sets/Pairs Description React Clone Isotype Apps Reg Format Size Cat. No. BrdU In-Situ Detection Kit II IHC(Zn), IHC(F), IHC(Fr) RUO Fixation Buffer, Diluent 200 tests 551321 Buffer, BD™ Retrievagen A antigen retrieval solution, biotinylated anti-BrdU antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) solution, DAB Buffer, DAB Chromogen, and Control Slides Caspase-3 Assay Kit SF, FA RUO PBS Buffer, Cell Lysis 100 tests 556485 Buffer, HEPES Buffer, Caspase-3 Fluorogenic Substrate, Caspase-3 Inhibitor Caspase-3, Active FA, SF RUO Set 20 tests 556473
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  • Cloning of the Human Homolog of the CDC34 Cell Cycle Gene by Complementation in Yeast SHARON E

    Cloning of the Human Homolog of the CDC34 Cell Cycle Gene by Complementation in Yeast SHARON E

    Proc. Natl. Acad. Sci. USA Vol. 90, pp. 10484-10488, November 1993 Genetics Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast SHARON E. PLON*tt, KATHLEEN A. LEPPIG§, HONG-NHUNG Do*, AND MARK GROUDINE*¶ *Fred Hutchinson Cancer Research Center, Seattle, WA 98104; and Departments of tMedicine, §Pathology and fRadiation Oncology, University of Washington, Seattle, WA 98195 Communicated by Leland Hartwell, August 9, 1993 ABSTRACT In a screen designed to isolate human cDNAs During initial screening for human cDNAs that would that complement a yeast G2 phase checkpoint mutation (mecl), complement the mecl checkpoint mutation, we obtained a we isolated a cDNA homologous to the Saccharomyces cerevi- partially active cDNA that further analysis reveals is the siae CDC34 gene. The human CDC34 cDNA can functionally human homolog of S. cerevisiae CDC34. We report here the substitute for the yeast CDC34 gene and represents a mam- cloning and functional and physical characterization of this malian homolog ofthe group ofyeast genes required for the late human gene. 11 G- S phase transition. The human CDC34 gene is expressed in multiple cell lines as a unique species and Southern blot MATERIALS AND METHODS analysis reveals evidence for a single gene that is highly conserved in higher eukaryotes. The human gene is located on Yeast and Bacterial Strains. The S. cerevisiae strains de- the far telomeric region of 19p13.3 in a location that defmes a scribed in these experiments were isogenic with A364a. region of homology between human chromosome 19p and Source of the strain other than this laboratory are indicated: mouse chromosome 11.