bioRxiv preprint doi: https://doi.org/10.1101/396515; this version posted August 20, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Nanopore sequence-based genome assembly of the basmati rice Jae Young Choi1, Simon C. Groen1, Sophie Zaaijer2, and Michael D. Purugganan1,3* 1Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York, USA 2New York Genome Center, New York, New York, USA 3Center for Genomics and Systems Biology, NYU Abu Dhabi Research Institute, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates * Corresponding author, Email:
[email protected] (MDP) Keywords: Oryza sativa, Asian rice, aromatic rice group, domestication, crop evolution, nanopore sequencing, aus, basmati, indica, japonica, admixture, awnless bioRxiv preprint doi: https://doi.org/10.1101/396515; this version posted August 20, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. ABSTRACT Basmati rice is an iconic cultivated Asian rice (Oryza sativa) variety group that is widespread in the Indian subcontinent. Despite its economic and cultural importance, a high-quality reference genome is currently lacking, and the group’s origins have not been fully resolved. To address these gaps we obtained 48x read coverage of a basmati genome using Oxford Nanopore Technologies’ long-read sequencing platform.