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Isolation and Identification of Some Pathogenic Bacteria Species from Contaminated Ambient with Oily Hydrocarbons

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Isolation and Identification of Some Pathogenic Bacteria Species from Contaminated Ambient with Oily Hydrocarbons Journal of Bacteriology & Mycology: Open Access Research Article Open Access Isolation and identification of some pathogenic bacteria species from contaminated ambient with oily hydrocarbons Abstract Volume 8 Issue 4 - 2020 Pathogenic species may naturally at nature in different conditions, at fields of petrol and contaminated area. The pathogenic species Citrobacter amalonaticus which is a genus Ahmed Ibrahim Jessim, Rasha Kifah Hassan, of coliform bacteria that is a gram-negative within the family of intestinal bacteria, were Ammar Salim Salman isolated from water samples 3, 4, and 5 of Al-Ahdab field in addition of presence Bacillus Ministry of Science and Technology, Center for research and subtilis, but pathogenic gram-negative species Sphingomonas paucimobilis bacteria found evaluation, Directorate of Treatment and disposal of chemical, as a biodegradable organism was identified and isolated from samples of soil from a Al- biological and military hazardous wastes, Iraq Ahdad filed 1 and 2, In addition of presence Pseudomonas aeruginosa in a soil sample Correspondence: Ahmed Ibrahim Jessim, Ministry of Science of Al-Gharaaf field at Al-Nnasiriyah in Iraq. These results were different from municipal and Technology, Center for research and evaluation, Directorate fuel of Al-Sidiyeh station, Brother’s station, and Al-Amel station for calibration, which of Treatment and disposal of chemical, biological and military shown just Bacillus spp., B. cereus, in general especially in polluted soil with diesel with no hazardous wastes, Iraq, Tel +9647713659713, presence of C. amalonaticus, and S. paucimobilis. Isolation and identification of bacterial Email depended on morphology using triple light microscope and biochemical tests. Furthermore we can use pathogenic microorganisms in biodegradation. Received: October 08, 2020 | Published: October 16, 2020 Keywords: isolation, diagnosis of bacteria, contaminated soil, oil products and pollution Introduction All different groups of living microorganisms such as bacteria, fungus and algae are represented in the soil and aquatic environments have Living microorganisms content of soils are a very important part an enzymatic ability for degradation of hydrocarbons.6 Petrol usually 1 of natural ecosystems, where they can adjust the flow of energy. As found as a complex mixture from kinds of hydrocarbons includes well as the cycle of materials by consumption of animal and plant aliphatic, mono-rings and multi rings alkanes, therefore studies wastes, these microorganisms play a pivotal role in the growth of of biodegradation are carried out on the base of bio prospecting at 2 agricultural plants and multiplication of agricultural crops. Also they contaminated sites in contaminated sites.9 In spite of the sensitivity can act a positive role in ecological soil system, in addition to the of bacteria towards environmental conditions, but found that there 3 bioremediation and to the transfer of organic substances. Moreover, are types of bacteria can be isolated from soils that have suffered microbial diversity in the soil is related closely to the function and from harsh environmental conditions, for example, Bacterial 4 structure of the surrounding ecosystem. In addition to that they are strains were isolated from these soils which they are Pseudomonas 5 considered as a component of maintaining soil productivity. As a aeruginosa JX962696.1, P. aeruginosa KC776528.1, P.aeruginosa result of ability of living microorganisms like bacteria, fungus, yeasts JF919950.1, Klebsiella pneumonia LC093517.1, Arthrobacter sp. 6 and microalgae to remove hydrocarbons complexes, where bacteria GU451067.1, Citrobacter sp. KR063563.1, Citrobacter amalonaticus are present in contaminated areas with oil derivatives and the nature of KC689297.1, Enterobacter helveticus, JN255127.1, Cronobacter specialized bacteria sometimes found in contaminated soils, the strain muytjensii JN255123.1, Citrobacter amalonaticus KC689297.1 and of Pseudomonas strain PS-1 has been found in these soils due to their Bacillus cereus LN890172.1 which are unexpected to be isolated 7 ability to degrade alkanes and aromatic compounds. In some places from contaminated soils from various sources including seashore near the dominance of microorganisms were found, for example, in Daqing a gas station and car workshops from deep of 1-2cm. results were oily fields in China, the dominance of Acidobacteria, Actinobacteria, not exaggerated because they were diagnosed molecularly and by 5 Bacteriocytes, Chlorogenic, Planctomycetes and Proteobacteria. sprinting sp73 and sp 74.10 In some regions, the dominance of one Furthermore, Enterobacter sp. CMG 457 was identified as a highly species on other species via the tests of ribosomal acid 16S rRNA resentence to organ hydrocarbons like aromatic, paraffin and pesticides and it has been sown the dominance of Bacillus species like Bacillus from oil pump in paradise habitation in Karachi city in Pakistan, in cereus, B. sphaericus , B. fusiformis, B. pumilus with less bacterial addition to their tolerant of 3mM of heavy metals like CuSO4, CdCl2 appearance of Acinetobacter junii, Pseudomonas sp. in polluted 8 11 and CrCl2. Also several recorded strains of bacteria which they soils with gas oil. Other conditions may determine the presence of are Aeromicrobium, Brevibacterium, Burkholderia, Gordonia and bacterial families such as acidity, alkalinity and may be implicitly Mycobacterium were isolated from oily contaminated soils, these specialized on some organic substances, for some individuals which bacteria shown a good ability to degrade hydrocarbons compounds, belong to the families of Azotobacteriaceae, Enterobacteriaceae, and in another study, three strains of bacteria Bacillus subtilis SA7, Pseudomonas, the ability to grow in contaminated environments with Pseudomonas aeruginosa SA3 sp. and Citrobacter SB9 beside of two Xylene, benzene and diphenylamine at 25-45ºC and pH=5-9, therefore species of microalgae Aphanocaps and Chlorella minutissimma. The they can be used in industrial remediation.12 There is a probability of fungus also has the ability and can degrade petrol like Amorphoteca, four known species have the ability to grow in contaminated soils 8 Candida, Graphium, Neosartorya, Pichia, Talaromyces and Yarrowia. with petrol which they are Bacillus, Micrococcus, Pseudomonas and Submit Manuscript | http://medcraveonline.com J Bacteriol Mycol Open Access. 2020;8(4):111‒115. 111 ©2020 Jessim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and build upon your work non-commercially. Isolation and identification of some pathogenic bacteria species from contaminated ambient with oily Copyright: 112 hydrocarbons ©2020 Jessim et al. Rhodococcus, these species have already grown on the apparent oil and Materials and methods some aromatic hydrocarbons that have to be able to extract carbon from these materials and may be due to adaptation on harsh environmental Soil samples were being taken from Al-Sidiyeh station, brother’s conditions.14 Despite the toxicity of Polycyclic aromatic hydrocarbons station in Al-Jihad neighborhood and Al-Amel station near the (PAHs), but it is possible to isolate different kinds of bacteria from distribution pumps of petroleum derivatives such as diesel, gasoline different contaminated sites with oil, these bacteria have the ability and kerosene, and then transferred to the laboratory for preparation of growing on different hydrocarbons like diesel, benzene, lubricants, and cultivation, and samples of soil of drilling and water associated toluene, naphthalene and kerosene. An isolated strains of bacteria are with crude oil as follows: often negative gram, sometimes has a very good ability to release I. Sample of soil from Al-Ahdab field energy from diesel, so it can be invested in bioremediation processes14. Some other bacterial species isolates belong to following families II. Sample of soil from Al-Ahdab field Pseudomonadaceae and Moraxellaceae (species Acinetobacter), were III. Water sample associated with drilling in the Al-Ahdab field known well with high ability to degrade hydrocarbons, therefore they can be stimulated for the purpose of bioremediation, the recent IV. Water sample associated with drilling in “ “ studies showed the species of Pseudomonas putida, Acinetobacter V. Water sample associated with drilling in “ “ calcoaceticus and Acinetobacter johnsonii have the ability to degrade naphthalene compounds and degrade related compounds VI. Soil sample belongs to Al-Gharaf fields in Nasiriyah Governorate with naphthalene ring, in other hand the ability of Acinetobacter is higher to decompose alkanes highly carbons compounds started from Media of soil extract agar and nutrient agar media, test tubes, C18 – C 24.15 Here we should know a little about aerobic degradation sterilized water media, water bath, Petri dishes gram stains and of petroleum compounds, resent studies reported the most rapid and malachite green are used to finish work complete remediation through degradation bath ways of the majority Procedure of organic pollutants, were passing through under aerobic conditions, An organic pollutants is an oxidative process initially are intracellular, One gm of each sample of soil are taken from surface soils of oil and as well as the activation incorporation of oxygen is enzymatic products stations and Al-Ahdab field were weighed, and 1 ml of water key of reaction
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