DOCSLIB.ORG

Fathead Minnow Vitellogenin: Complementary Dna Sequence and Messenger Rna and Protein Expression After 17␤-Estradiol Treatment

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Fathead Minnow Vitellogenin: Complementary Dna Sequence and Messenger Rna and Protein Expression After 17␤-Estradiol Treatment Environmental Toxicology and Chemistry, Vol. 19, No. 4, pp. 972±981, 2000 Printed in the USA 0730-7268/00 $9.00 1 .00 FATHEAD MINNOW VITELLOGENIN: COMPLEMENTARY DNA SEQUENCE AND MESSENGER RNA AND PROTEIN EXPRESSION AFTER 17b-ESTRADIOL TREATMENT JOSEPH J. KORTE,*² MICHAEL D. KAHL,² KATHLEEN M. JENSEN,² MUMTAZ S. PASHA,² LOUISE G. PARKS,³ GERALD A. LEBLANC,³ and GERALD T. A NKLEY² ²U.S. Environmental Protection Agency, Mid-Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804 ³North Carolina State University, Department of Toxicology, Box 7633, Raleigh, North Carolina 27695, USA (Received 9 April 1999; Accepted 9 August 1999) AbstractÐInduction of vitellogenin (VTG) in oviparous animals has been proposed as a sensitive indicator of environmental contaminants that activate the estrogen receptor. In the present study, a sensitive ribonuclease protection assay (RPA) for VTG messenger RNA (mRNA) was developed for the fathead minnow (Pimephales promelas), a species proposed for routine endocrine- disrupting chemical (EDC) screening. The utility of this method was compared with an enzyme-linked immunosorbent assay (ELISA) speci®c for fathead minnow VTG protein. Assessment of the two methods included kinetic characterization of the plasma VTG protein and hepatic VTG mRNA levels in male fathead minnows following intraperitoneal injections of 17b-estradiol (E2) at two dose levels (0.5, 5.0 mg/kg). Initial plasma E2 concentrations were elevated in a dose-dependent manner but returned to normal levels within 2 d. Liver VTG mRNA was detected within 4 h, reached a maximum around 48 h, and returned to normal levels in about 6 d. Plasma VTG protein was detectable within 16 h of treatment, reached maximum levels at about 72 h, and remained near these maximum levels for at least 18 d. While the RPA was about 1,000 times more sensitive than the ELISA, the ELISA appears superior for routine screening tests. The ELISA method is relatively simple to perform and, because males lack a clearance mechanism for VTG, the protein remains at relatively high concentrations in the plasma for an extended period of time. As part of the development of the RPA, the complementary DNA (cDNA) sequence for fathead minnow VTG was determined and the deduced amino acid sequence compared with VTG sequences for other ®sh species. KeywordsÐVitellogenin Pimephales promelas 17b-Estradiol mRNA cDNA INTRODUCTION for screening EDCs is international in scope, with Organization Concern for possible adverse effects of endocrine-disrupt- for Economic Cooperation and Development member coun- ing chemicals (EDCs) on humans and wildlife prompted the tries working on methods development and test harmonization U.S. Environmental Protection Agency to identify research on with this species [8]. this topic as one of six high-priority issues [1]. Coincident There are a number of existing assay protocols with the with this, the U.S. Environmental Protection Agency received fathead minnow, including partial- and full-life cycle tests [9± a legislated mandate to develop and implement a screening 12], that would capture adverse effects on estrogen, androgen, program for detecting potential EDCs [2,3]. To meet this man- and/or thyroid function. However, the endpoints typically as- date, a number of expert workshops were initiated to help sessed in these tests (e.g., growth rate, developmental anom- identify potential screening methods [4±6], and a multistake- alies, reproductive success) are also re¯ective of potential im- holder advisory group was formed to provide recommenda- pacts associated with a variety of other toxicity mechanisms. tions to the U.S. Environmental Protection Agency for devel- Hence, in developing fathead minnow screening methods spe- oping and implementing an EDC screening program. This ci®c for EDCs, there has been an emphasis on assessment of group, the Endocrine Disruptor Screening and Testing Advi- biological endpoints that are directly related to mechanisms sory Committee, developed a list of screening assays suitable of concern. For example, the protocol recommended for tier for tier 1 (initial) testing for chemicals that could cause toxicity 1 screening incorporates assessment of plasma vitellogenin through disruption of reproductive and developmental pro- (VTG) concentrations in test animals [5,7]. Under normal con- cesses controlled by estrogen, androgen, and thyroid hormones ditions, the production of VTG, a precursor to egg yolk protein, [7]. The tier 1 battery recommended by the Endocrine Dis- is induced in the liver of oviparous females by the interaction ruptor Screening and Testing Advisory Committee includes of estrogens with the estrogen receptor (ER) [13,14]. Males three mammalian tests, one amphibian assay (with Xenopus also possess the gene for VTG, but plasma concentrations of laevis), and a test with the fathead minnow (Pimephales pro- the protein typically remain small, presumably due to low melas). Although the most immediate application is in the levels of endogenous estrogens [15,16]. However, exposure of United States, interest in using the fathead minnow as a model males to exogenous estrogens/estrogen mimics can result in a marked induction of plasma VTG, which tends to remain el- * To whom correspondence may be addressed evated because of lack of mechanisms to clear the protein[17]. ([email protected]). This has been successfully exploited as a biomarker of ex- This manuscript has been reviewed in accordance with of®cial posure of ®sh to ER agonists in the environment [e.g., 18± U.S. Environmental Protection Agency (U.S. EPA) procedures; how- ever, the content does not re¯ect U.S. EPA policy. Mention of trade 23]. Further, a number of studies have demonstrated a corre- names does not imply endorsement by the U.S. EPA or the U.S. lation between VTG induction and adverse reproductive effects government. both in male and female ®sh [24±28]. 972 Expression of fathead minnow vitellogenin mRNA and protein Environ. Toxicol. Chem. 19, 2000 973 There are a number of techniques available for measuring compared with E2 standards. Extraction ef®ciency, as deter- VTG. An effective, but relatively nonspeci®c, measure of VTG mined by recovery of tritiated steroids extracted from plasma, can be made through assaying alkaline-labile protein-bound was 85 to 90% (data not shown). phosphorus in ®sh plasma [17,27,29]. More speci®c measure- ment techniques for VTG include radioimmunoassays and en- Plasma vitellogenin measurement zyme-linked immunosorbent assays (ELISA); these approach- Plasma VTG protein was measured using a competitive es have been used successfully for measuring fathead minnow ELISA with an antibody directed against fathead minnow VTG VTG [8,16,28,30]. It also is possible to assess events asso- [30]. Brie¯y, puri®ed fathead minnow VTG was serially di- ciated with VTG induction further upstream through mea- luted to obtain standard concentrations between 6 and 1,500 surement of VTG mRNA [31,32]; however, this approach has ng/ml. These standards and dilutions of plasma between 1:325 not been commonly utilized, in part, because of lack of a (controls and samples with little VTG expected) and 1:675,000 standard technique for measuring VTG mRNA. The purpose (treated samples with high VTG expected) were combined with of the present study, therefore, was to develop and validate a an equal volume of a 1:40,000 dilution of primary antibody ribonuclease protection assay (RPA) for measuring VTG and incubated for1hat378C. Two hundred-microliter aliquots mRNA in the fathead minnow. As part of this study, we as- were removed in duplicate, added to 96-well microtiter plates sessed the kinetics of mRNA versus protein induction in male that had been coated with fathead minnow VTG, and incubated ®sh treated with 17b-estradiol (E2). In conjunction with de- for1hat378C. After washing the plates, a 1:40,000 or 1: velopment of the RPA, we also were able to completely se- 80,000 dilution of goat antirabbit peroxidase-conjugated sec- quence the fathead minnow VTG complementary DNA ondary antibody (Bio-Rad, Hercules, CA, USA) was added (cDNA) and compare the deduced amino acid sequence to and incubated for1hat378C. After washing the plates again, published sequences for other ®sh species. the peroxidase substrate was added and allowed to react for 5 to 10 min. The reaction was stopped with 100 mlof1M MATERIALS AND METHODS phosphoric acid and the response measured using a 450-nm Treatment of ®sh ®lter in a plate reader (Model 3550, Bio-Rad). Some samples were reassayed at new dilutions because the values obtained To characterize induction of VTG mRNA and protein, adult were outside the reliable range of the standards. (seven±nine-month-old) male fathead minnows from on-site cultures were anesthetized via water exposure to tricaine meth- Preparation of probe and target RNA for the ribonuclease anesulfonate (100 mg/L) buffered with NaHCO3 (200 mg/L) protection assay and given intraperitoneal (ip) injections of E2 in 10% (v/v) Detailed presentation of the principles and procedures in- ethanol in corn oil. The ®sh were injected at 10 l/g of body m volved in the development and use of the RPA for measure- weight to achieve E2 doses of 5.0 (high) and 0.5 mg/kg (low). ment of speci®c mRNA sequences can be found in the manual Control ®sh received injections of the ethanol-corn oil only. for the Ambion RPA IITM kit (Austin, TX, USA). The ®rst step After injection, the ®sh were held in 10-L tanks receiving 150 in development of the RPA was to obtain a probe that had the ml/min of sand-®ltered Lake Superior water at 25 C. The pho- 8 complementary sequence for the VTG mRNA. To achieve this, toperiod was 16:8 light:dark, and ®sh were fed frozen brine we isolated total RNA from a pool of livers from seven fathead shrimp (San Francisco Bay Brand, San Francisco, CA, USA) minnows 48 h after an ip injection of E2 at 5 mg/kg (see daily.
Load more

Recommended publications
  • Quantity Does Matter. Juvenile Hormone and the Onset of Vitellogenesis in the German Cockroach J
    Insect Biochemistry and Molecular Biology 33 (2003) 1219–1225 www.elsevier.com/locate/ibmb Quantity does matter. Juvenile hormone and the onset of vitellogenesis in the German cockroach J. Cruz, D. Martı´n, N. Pascual, J.L. Maestro, M.D. Piulachs, X. Belle´s ∗ Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona (CSIC), Jordi Girona 18, 08034 Barcelona, Spain Received 7 February 2003; received in revised form 18 May 2003; accepted 28 June 2003 Abstract We aimed to elucidate why cockroaches do not produce vitellogenin in immature stages, by studying the appearance of vitellog- enin mRNA in larvae of Blattella germanica. Treatment of female larvae in any of the last three instars with 1 µg of juvenile hormone (JH) III induces vitellogenin gene transcription, which indicates that the fat body is competent to transcribe vitellogenin at least from the antepenultimate instar larvae. In untreated females, vitellogenin production starts on day 1 after the imaginal molt, when corpora allata begin to synthesize JH III at rates doubling the maximal of larval stages. This coincidence suggests that the female reaches the threshold of JH production necessary to induce vitellogenin synthesis on day 1 of adult life. These data lead to postulate that larvae do not synthesize vitellogenin simply because they do not produce enough JH, not because their fat body is incompetent. 2003 Elsevier Ltd. All rights reserved. Keywords: Vitellogenin; Juvenile hormone; German cockroach; Blattella germanica; Reproduction; Metamorphosis; Ecdysteroids 1. Introduction most insect groups, which start vitellogenesis after the imaginal molt. Why, then, do immature insects not pro- In practically all insect species, vitellogenesis and duce vitellogenin? It is because the genes coding for vit- oocyte growth are restricted to the adult stage.
    [Show full text]
  • Biochemical Analysis of Vitellogenin from Rainbow Trout (Salmo Gairdneri) : Fatty Acid Composition of Phospholipids Lucie Fremont, A
    Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids Lucie Fremont, A. Riazi To cite this version: Lucie Fremont, A. Riazi. Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids. Reproduction Nutrition Développement, 1988, 28 (4A), pp.939-952. hal-00898891 HAL Id: hal-00898891 https://hal.archives-ouvertes.fr/hal-00898891 Submitted on 1 Jan 1988 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids Lucie FREMONT, A. RIAZI Station de Recherches de Nutrition, /.N.R.A., 78350 Jouy-en-Josas, France. Summary. Vitellogenin was obtained from three year-old vitellogenic trout. Two procedures of isolation were compared : dialysis against distilled water and ultracentrifugation in the density interval 1 .21 -1 .28 g/ml. Similar patterns were observed by gel filtration and electrophoresis for both prepara- tions of vitellogenin, indicating that electric charge and molecular weight were not modified by either procedure. The apparent M, of the native form was 560,000 in gel filtration, whereas that of the monomer was estimated as 170,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis.
    [Show full text]
  • Brood Pheromone Suppresses Physiology of Extreme Longevity in Honeybees (Apis Mellifera)
    3795 The Journal of Experimental Biology 212, 3795-3801 Published by The Company of Biologists 2009 doi:10.1242/jeb.035063 Brood pheromone suppresses physiology of extreme longevity in honeybees (Apis mellifera) B. Smedal1, M. Brynem2, C. D. Kreibich1 and G. V. Amdam1,3,* 1Department of Chemistry, Biotechnology and Food Science, University of Life Sciences, P.O. Box 5003, N-1432 Aas, Norway, 2Department of Animal and Aquacultural Sciences, University of Life Sciences, P.O. Box 5003, N-1432 Aas, Norway and 3School of Life Science, Arizona State University, Tempe, P.O. Box 874501, AZ 85287, USA. *Author for correspondence ([email protected]) Accepted 19 September 2009 SUMMARY Honeybee (Apis mellifera) society is characterized by a helper caste of essentially sterile female bees called workers. Workers show striking changes in lifespan that correlate with changes in colony demography. When rearing sibling sisters (brood), workers survive for 3–6 weeks. When brood rearing declines, worker lifespan is 20 weeks or longer. Insects can survive unfavorable periods on endogenous stores of protein and lipid. The glyco-lipoprotein vitellogenin extends worker bee lifespan by functioning in free radical defense, immunity and behavioral control. Workers use vitellogenin in brood food synthesis, and the metabolic cost of brood rearing (nurse load) may consume vitellogenin stores and reduce worker longevity. Yet, in addition to consuming resources, brood secretes a primer pheromone that affects worker physiology and behavior. Odors and odor perception can influence invertebrate longevity but it is unknown whether brood pheromone modulates vitellogenin stores and survival. We address this question with a 2-factorial experiment where 12 colonies are exposed to combinations of absence vs presence of brood and brood pheromone.
    [Show full text]
  • Insect Vitellogenin/Yolk Protein Receptors Thomas W
    Entomology Publications Entomology 2005 Insect Vitellogenin/Yolk Protein Receptors Thomas W. Sappington U.S. Department of Agriculture, [email protected] Alexander S. Raikhel University of California, Riverside Follow this and additional works at: https://lib.dr.iastate.edu/ent_pubs Part of the Entomology Commons, and the Population Biology Commons The ompc lete bibliographic information for this item can be found at https://lib.dr.iastate.edu/ ent_pubs/483. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Book Chapter is brought to you for free and open access by the Entomology at Iowa State University Digital Repository. It has been accepted for inclusion in Entomology Publications by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Insect Vitellogenin/Yolk Protein Receptors Abstract The protein constituents of insect yolk are generally, if not always, synthesized outside the oocyte, often in the fat body and sometimes in the foilicular epithelium (reviewed in Telfer, 2002). These yolk protein precursors (YPP's) are internalized by the oocyte through receptor-mediated endocytosis (Roth et al., 1976; Telfer et al., 1982; Raikhel and Dhaclialla, 1992; Sappington and Rajkhel, 1995; Snigirevskaya et al., I 997a,b). A number of proteins have been identified as constituents of insect yolk (reviewed in Telfer, 2002), and some of their receptors have been identified. The at xonomically most wide pread class of major YPP in insects and other oviparous animals is vitellogenin (Vg). Although several insect Vg receptors (VgR) have been characterized biochemically, as of this writing there are only two insects from which VgR sequences have been reported, including the yellowfevcr mosquito (A edes aegypt1) (Sappington et al., 1996; Cho and Raikhel, 2001 ), and the cockroach (Periplaneta americana) (Acc.
    [Show full text]
  • Vitellogenic Proteins: Endocrine Regulated Biosynthesis
    Vitellogenic Proteins: Endocrine Regulated Biosynthesis Vitellogenic proteins (vitellogenins) synthesized by extraovarian tissues are known from animals phylogenetically as far apart as birds, amphibians, reptiles, crustaceans, and insects. All produce yolky eggs within short time intervals. Several aspects in the machin- ery of yolk precursor synthesis are common to most of these animals, a fact that makes a comparative study a fruitful approach. Among these are synthesis of the yolk precursor exclusively by the female of the species in most cases, and the involvement of hormones in the specific protein synthesis. Sex specificity finds its expression in the term female specific protein, often used and quite appropriate. However, there are a few exceptions to this and consequently the term vitellogenin has been coined to describe a class of proteins which is preferentially taken up by the oocytes against a concentration gradient. This term Downloaded from https://academic.oup.com/icb/article/14/4/1159/2069954 by guest on 28 September 2021 is also not quite accurate, since many of the blood proteins contribute to the yolk protein pool as well, evea though only to a minor extent. Yet the term vitellogenin is conveniently used. The specificity of the vitellogenic proteins, immunologically identifiable, provides a useful tool for a detailed analysis of induction of a protein and the mode of hormone action on several levels in this process. The vitellogenic systems are potential model systems for the studies of hormonal control of protein biosynthesis. In recent years, considerable progress has been made on several aspects of vitellogenin synthesis in divers animals. These include the isolation and identification of the specific protein, hormonal involvement, and mode of hormone action.
    [Show full text]
  • Roles of Estrogens in the Healthy and Diseased Oviparous Vertebrate Liver
    H OH metabolites OH Review Roles of Estrogens in the Healthy and Diseased Oviparous Vertebrate Liver Blandine Tramunt 1,2, Alexandra Montagner 1, Nguan Soon Tan 3 , Pierre Gourdy 1,2 , Hervé Rémignon 4,5 and Walter Wahli 3,5,6,* 1 Institut des Maladies Métaboliques et Cardiovasculaires (I2MC-UMR1297), INSERM/UPS, Université de Toulouse, F-31432 Toulouse, France; [email protected] (B.T.); [email protected] (A.M.); [email protected] (P.G.) 2 Service de Diabétologie, Maladies Métaboliques et Nutrition, CHU de Toulouse, F-31059 Toulouse, France 3 Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, Singapore 308232, Singapore; [email protected] 4 INP-ENSAT, Université de Toulouse, F-31320 Castanet-Tolosan, France; [email protected] 5 Toxalim Research Center in Food Toxicology (UMR 1331), INRAE, National Veterinary College of Toulouse (ENVT), Purpan College of Engineers of the Institut National Polytechnique de Toulouse (INP-PURPAN), Université Toulouse III—Paul Sabatier (UPS), Université de Toulouse, F-31300 Toulouse, France 6 Center for Integrative Genomics, Université de Lausanne, Le Génopode, CH-1015 Lausanne, Switzerland * Correspondence: [email protected] Abstract: The liver is a vital organ that sustains multiple functions beneficial for the whole organism. It is sexually dimorphic, presenting sex-biased gene expression with implications for the phenotypic differences between males and females. Estrogens are involved in this sex dimorphism and their actions in the liver of several reptiles, fishes, amphibians, and birds are discussed. The liver partici- pates in reproduction by producing vitellogenins (yolk proteins) and eggshell proteins under the Citation: Tramunt, B.; Montagner, A.; control of estrogens that act via two types of receptors active either mainly in the cell nucleus (ESR) Tan, N.S.; Gourdy, P.; Rémignon, H.; or the cell membrane (GPER1).
    [Show full text]
  • The Nurse's Load: Early-Life Exposure to Brood-Rearing Affects
    The nurse’s load: Early-life exposure to brood-rearing affects behavior and lifespan in honey bees (Apis mellifera) By: Gro V. Amdam, Olav Rueppell, M. Kim Fondrk, Robert E. Page , C. Mindy Nelson Amdam G.V., Rueppell O., Fondrk M.K., Page R.E. Jr., Nelson C.M. (2009) The nurse‟s load: early-life exposure to brood-rearing affects behavior and lifespan in honey bees (Apis mellifera). Experimental Gerontology, 44(6/7), 447-452 Made available courtesy of Elsevier: http://www.elsevier.com/ ***Reprinted with permission. No further reproduction is authorized without written permission from Elsevier. This version of the document is not the version of record. Figures and/or pictures may be missing from this format of the document.*** Abstract: Long-lived honey bees (Apis mellifera) develop in fall. This pattern may be explained by reduced nurse loads. When the amount of brood in colonies declines as a function of adverse foraging conditions, adult bees build up surplus nutrient stores that include vitellogenin, a behavioral affector protein that also can increase lifespan. Although the seasonal reduction in exposure to nursing tasks predictably results in vitellogenin accumulation, the assumption that long-lived adults thereby develop is confounded by a concomitant decline in foraging effort. Foraging activity reduces lifespan, and is influenced by colony resource consumption, brood pheromones, availability of nectar and pollen, and weather. Here, we perform the first controlled experiment where the nursing environment of pre-foraging sister bees was set to vary, while their foraging environment later was set to be the same. We measure vitellogenin, age at foraging onset and lifespan.
    [Show full text]
  • Of Very Low Density Lipoprotein and Vitellogenin (Multifunctional Receptors/Cell Growth/Endocytosis) STEFANO STIFANI, DWAYNE L
    Proc. Natl. Acad. Sci. USA Vol. 87, pp. 1955-1959, March 1990 Cell Biology A single chicken oocyte plasma membrane protein mediates uptake of very low density lipoprotein and vitellogenin (multifunctional receptors/cell growth/endocytosis) STEFANO STIFANI, DWAYNE L. BARBER, JOHANNES NIMPF, AND WOLFGANG J. SCHNEIDER* Department of Biochemistry and Lipid and Lipoprotein Research Group, University of Alberta, Edmonton, AB T6G 2S2, Canada Communicated by Daniel Steinberg, December 18, 1989 ABSTRACT Specific cell-surface receptors mediate the tions), we observed that high concentrations of unlabeled uptake of plasma proteins into growing oocytes of oviparous VTG and VLDL competed with the binding of both '251- species, thereby forming yolk. Quantitatively the most impor- labeled VTG and VLDL to chicken oocyte membrane ex- tant yolk precursors are the lipoproteins, very low density tracts, further suggesting the presence of one bifunctional lipoprotein, and vitellogenin. We show that a single major receptor. In the light ofthe pivotal role of receptor-mediated chicken oocyte plasma membrane protein with an apparent endocytosis of yolk proteins in the reproductive effort of the molecular mass of95 kDa as determined by SDS/PAGE under hen, coupled to the inability of the oocyte to synthesize yolk nonreducing conditions is the receptor for both ofthese ligands. proteins (3), it seemed reasonable to us that one and the same Binding activities for the two ligands copurified on ligand chicken oocyte plasma membrane receptor would be respon- affinity matrices and were inhibited by the same antibody sible for the import ofthe major yolk lipoproteins, VLDL and preparations, and the ligands competed with each other for VTG.
    [Show full text]
  • Juvenile Hormone Downregulates Vitellogenin Production in Ectatomma Tuberculatum
    © 2016. Published by The Company of Biologists Ltd | Journal of Experimental Biology (2016) 219, 103-108 doi:10.1242/jeb.127712 RESEARCH ARTICLE Juvenile hormone downregulates vitellogenin production in Ectatomma tuberculatum (Hymenoptera: Formicidae) sterile workers Dihego Oliveira Azevedo1,Sérgio Oliveira de Paula2, JoséCola Zanuncio3, Luis Carlos Martinez2 and JoséEduardo Serraõ2,* ABSTRACT 2008), antioxidation (Seehuus et al., 2006), immunity regulation In the ant Ectatomma tuberculatum (Olivier 1792), workers have (Amdam et al., 2004; Seehuus et al., 2006), juvenile hormone (JH) active ovaries and lay trophic eggs that are eaten by the queen synthesis (Guidugli et al., 2005) and insulin/insulin-like signaling, and larvae. Vitellogenins are the main proteins found in the eggs which controls growth, aging and reproduction (Corona et al., of insects and are the source of nutrients for the embryo in the 2007). In worker ants, vitellogenin is associated with age fertilized eggs and for adults in the trophic eggs. In social insects, polyethism (Fénéron et al., 1996; Azevedo et al., 2011), the vitellogenin titres vary between castes and affect reproductive nourishment of larvae and adults by the production of trophic eggs social status, nursing, foraging, longevity, somatic maintenance, (Camargo Mathias and Caetano, 1995; Fénéron and Billen, 1996; and immunity. In most insects, vitellogenin synthesis is mainly Peeters, 1997; Gobin et al., 1998; Dietemann and Peeters, 2000; regulated by juvenile hormone. However, in non-reproductive Khila and Abouheif, 2008) and polyphenism (Libbrecht et al., worker ants, this relationship is poorly characterized. This study 2013). determined the effects of juvenile hormone on vitellogenin Vitellogenin dynamics in adult insects is closely related to JH synthesis in non-reproductive E.
    [Show full text]
  • Vitellogenin, Juvenile Hormone, Insulin Signaling, and Queen Honey Bee Longevity
    Vitellogenin, juvenile hormone, insulin signaling, and queen honey bee longevity Miguel Corona*, Rodrigo A. Velarde*, Silvia Remolina†, Adrienne Moran-Lauter‡, Ying Wang§, Kimberly A. Hughes†¶, and Gene E. Robinson*‡¶ʈ Departments of *Entomology, †Animal Biology, and §Cell and Developmental Biology, and ‡Neuroscience Program, and ¶Institute for Genomic Biology, University of Illinois at Urbana–Champaign, 320 Morrill Hall, 505 South Goodwin Avenue, Urbana, IL 61801 Contributed by Gene E. Robinson, March 1, 2007 (sent for review November 23, 2006) In most animals, longevity is achieved at the expense of fertility, follow an opposite pattern (12). In queens, both JH and Vg but queen honey bees do not show this tradeoff. Queens are both hemolymph titers are elevated in emerging virgin queens, but long-lived and fertile, whereas workers, derived from the same whereas JH drops and stays low thereafter (15), Vg remains high genome, are both relatively short-lived and normally sterile. It has (12). Vg gene expression agrees with protein measurements (16– been suggested, on the basis of results from workers, that vitel- 18) but has not been studied in old queens. logenin (Vg), best known as a yolk protein synthesized in the Recent results from studies using worker bees (7) suggest that the abdominal fat body, acts as an antioxidant to promote longevity in unique negative relationship between JH and Vg may be important queen bees. We explored this hypothesis, as well as related roles to our understanding of queen longevity. Vg has antioxidant of insulin–IGF-1 signaling and juvenile hormone. Vg was expressed functions in workers, which led to the suggestion that Vg also in thorax and head fat body cells in an age-dependent manner, operates to increase queen lifespan (7).
    [Show full text]
  • Mechanisms Regulating Swarming Behavior in Honey Bees (Apis Spp.) Christina Grozinger, Jessica Richards, Heather Mattila
    From molecules to societies: mechanisms regulating swarming behavior in honey bees (Apis spp.) Christina Grozinger, Jessica Richards, Heather Mattila To cite this version: Christina Grozinger, Jessica Richards, Heather Mattila. From molecules to societies: mechanisms regulating swarming behavior in honey bees (Apis spp.). Apidologie, Springer Verlag, 2014, 45 (3), pp.327-346. 10.1007/s13592-013-0253-2. hal-01234732 HAL Id: hal-01234732 https://hal.archives-ouvertes.fr/hal-01234732 Submitted on 27 Nov 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Apidologie (2014) 45:327–346 Review article * INRA, DIB and Springer-Verlag France, 2013 DOI: 10.1007/s13592-013-0253-2 From molecules to societies: mechanisms regulating swarming behavior in honey bees (Apis spp.) 1 1 2 Christina M. GROZINGER , Jessica RICHARDS , Heather R. MATTILA 1Department of Entomology, Center for Pollinator Research, The Pennsylvania State University, 4A Chemical Ecology Lab, Orchard Road, University Park, PA 16802, USA 2Department of Biological Sciences, Wellesley College, 106 Central Street, Wellesley, MA 02481, USA Received 16 July 2013 – Revised 18 October 2013 – Accepted 25 October 2013 Abstract – Reproduction by colony fission, or swarming, is a spectacular example of a behavior that requires the simultaneous coordination of the activities of thousands of honey bee workers and their queen.
    [Show full text]
  • Isolation of Chicken Vitellogenin I and III Cdnas and the Developmental Resulation of Five Estrogen-Responsive Genes M the Embryonic Liver
    Downloaded from genesdev.cshlp.org on September 23, 2021 - Published by Cold Spring Harbor Laboratory Press Isolation of chicken vitellogenin I and III cDNAs and the developmental resulation of five estrogen-responsive genes m the embryonic liver Marilyn I. Evans, Rosane Silva, 1 and John B.E. Burch 2 Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 USA The isolation of cDNA clones that code for portions of the two minor chicken vitellogenin (VTG) genes (VTGI and VTGIII) is reported. These clones represent unique sequences that are expressed exclusively in the livers of estrogenized birds. In the liver of the egg-laying hen, the levels of RNAs encoding VTGI, VTGII, and VTGIII are approximately 11,000, 30,000, and 3,000 molecules per cell, respectively. We have used the newly isolated clones, as well as the yolk protein cDNAs previously available [VTGII, apolipoprotein II (apoVLDLII), and apolipoprotein B], as probes to examine several aspects of the regulation of these genes by estradiol. First, we demonstrate that the capacity of each gene to respond to estradiol is acquired between 8 and 13 days in ovo. The response of four of these genes to estradiol is diminshed during late fetal development, but the responsiveness is recovered within a week after hatching. Second, we demonstrate that these genes display distinct kinetic response profiles following the addition of estradiol. Third, as has been described previously for the VTGII and apoVLDLII genes, we demonstrate that a single injection of estradiol effects a long-term reprogramming event (hepatic memory) that allows a faster onset of the rapid accumulation of both VTGI and VTGIII RNAs following a subsequent rechallenge by estradiol.
    [Show full text]