Human ACY1 ELISA Matched Antibody Pair

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Version 01-06/20 User's Manual Human ACY1 ELISA Matched Antibody Pair ABPR-0012 This product is for research use only and is not intended for diagnostic use. For illustrative purposes only. To perform the assay the instructions for use provided with the kit have to be used. Creative Diagnostics Address: 45-1 Ramsey Road, Shirley, NY 11967, USA Tel: 1-631-624-4882 (USA) 44-161-818-6441 (Europe) Fax: 1-631-938-8221 Email: [email protected] Web: www.creative-diagnostics.com Cat: ABPR-0012 Human ACY1 ELISA Matched Antibody Pair Version 21-06/20 PRODUCT INFORMATION Intended Use This antibody pair set comes with matched antibody pair to detect and quantify protein level of human ACY1. General Description This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L- amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID: 100526760. A related pseudogene has been identified on chromosome 18. Reagents And Materials Provided Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-ACY1 (100 μg) 2. Detection antibody: rabbit purified polyclonal anti-ACY1 (50 μg) Note: Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols. Reconstitution And Storage Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use. Tel: 1-631-624-4882 (USA) Fax: 1-631-938-8221 Tel: 44-161-818-6441 (Europe) Email: [email protected] 2/2.

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Human ACY1 / Aminoacylase-1 Protein (His Tag)
Human ACY1 / Aminoacylase-1 Protein (His Tag) Catalog Number: 10549-H08B General Information SDS-PAGE: Gene Name Synonym: ACY-1; ACY1D; HEL-S-5 Protein Construction: A DNA sequence encoding the full length of human ACY1 (NP_000657.1) (Met 1-Ser 408) was expressed with a polyhistidine tag at the C-terminus. Source: Human Expression Host: Baculovirus-Insect Cells QC Testing Purity: > 95 % as determined by SDS-PAGE Endotoxin: Protein Description < 1.0 EU per μg of the protein as determined by the LAL method Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked Stability: ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the Samples are stable for up to twelve months from date of receipt at -70 ℃ two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 Predicted N terminal: Met activity measured in vitro, the C-terminal fragment inhibits while full-length Molecular Mass: ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its The recombinant human ACY1 consists of 419 amino acids and predicts a physiological functions. As a homodimeric zinc-binding enzyme, molecular mass of 47.3 kDa. It migrates as an approximately 44 kDa Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. protein in SDS-PAGE under reducing conditions. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is Formulation: characterized by accumulation of N-acetyl amino acids in the urine.
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Analysis of the Complex Interaction of Cdr1as‑Mirna‑Protein and Detection of Its Novel Role in Melanoma
ONCOLOGY LETTERS 16: 1219-1225, 2018 Analysis of the complex interaction of CDR1as‑miRNA‑protein and detection of its novel role in melanoma LIHUAN ZHANG*, YUAN LI*, WENYAN LIU, HUIFENG LI and ZHIWEI ZHU College of Life Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, P.R. China Received May 15, 2017; Accepted April 9, 2018 DOI: 10.3892/ol.2018.8700 Abstract. Despite improvements in the prevention, diagnosis has been detected in several species, including viruses (2), and treatment of melanoma having developed rapidly, the role plants (4), archaea (5)[Salzman, 2013 #198; Memczak, 2013 of circular RNA CDR1 antisense RNA (CDR1as) in melanoma #291] and animals (6). In eukaryotic cells, circRNA has remains to be elucidated. The aim of the present study was to attracted attention due to its unique characteristics, including predict the novel roles of CDR1as in melanoma through novel high stability, specificity and evolutionary conservation (7). bioinformatics analysis. In the present study, the circ2Traits The majority of circRNAs are derived from exons of coding database was used to supply information on CDR1as in cancer. regions, 3'UTRs, 5'UTRs, introns, intergenetic regions and CircNet, circBase and circInteractome databases were used to antisense RNAs (8). CircRNAs, which have attracted attention detect the co-expression of CDR1as, microRNAs and proteins. in recent years, can be produced by canonical and nonca- Furthermore, the functions and pathways of the associated nonical splicing as distinguished from the linear RNAs (9). proteins were predicted using the Database for Annotation, Through high-throughput sequencing, three types of circRNAs Visualization and Integrated Discovery. Gene Ontology have been identified: Exonic circRNAs (7), circular intronic enrichment analysis suggested that the proteins associated RNAs (ciRNAs) (9), and retained-intron circular RNAs or with CDR1as were mainly regulated in the cytoplasm as exon-intron circRNAs (elciRNAs) (10).
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Polymorphic Human Somatostatin Gene Is Located on Chromosome 3 (Gene Mapping/Somatic Cell Hybrids/DNA Polymorphism) SUSAN L
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Functional Annotation of the Human Retinal Pigment Epithelium
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Study of the Expression and Function of ACY1 in Patients with Colorectal Cancer
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Human Aminoacylase/ACY1 Antibody Catalog Number: ATGA0329
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Anti-ACY1 / Aminoacylase 1 Antibody (ARG40257)
Product datasheet [email protected] ARG40257 Package: 100 μl anti-ACY1 / Aminoacylase 1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes ACY1 / Aminoacylase 1 Tested Reactivity Hu, Ms, Rat Tested Application ICC/IF, IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name ACY1 / Aminoacylase 1 Antigen Species Human Immunogen Recombinant fusion protein corresponding to aa. 1-408 of Human ACY1 / Aminoacylase 1 (NP_001185824.1). Conjugation Un-conjugated Alternate Names ACY-1; N-acyl-L-amino-acid amidohydrolase; ACY1D; EC 3.5.1.14; HEL-S-5; Aminoacylase-1 Application Instructions Application table Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control A431, Mouse kidney and Rat kidney Calculated Mw 46 kDa Observed Size ~ 45 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw www.arigobio.com 1/3 cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol ACY1 Gene Full Name aminoacylase 1 Background This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids.
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Human Aminoacylase/ACY1 Antibody Antigen Affinity-Purified Polyclonal Goat Igg Catalog Number: AF2900
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ACY1 Polyclonal Antibody Product Information
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Associated Gene
Natural human genetic variation determines basal and inducible expression of PM20D1, an obesity- associated gene Kiara K. Bensona,b,c, Wenxiang Hua,b,c, Angela H. Wellera,b,c, Alexis H. Bennetta,b,c,1, Eric R. Chena,b,c,2, Sumeet A. Khetarpala,b,c,3, Satoshi Yoshinoa,b,c,4, William P. Boned,e,f, Lin Wangg,h, Joshua D. Rabinowitzg,h, Benjamin F. Voightd,e,f, and Raymond E. Soccioa,b,c,5 aDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; bDivision of Endocrinology, Diabetes, and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; cInstitute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; dDepartment of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; eDepartment of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; fInstitute of Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; gDepartment of Chemistry, Princeton University, Princeton, NJ 08544; and hLewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544 Edited by Bruce M. Spiegelman, Dana-Farber Cancer Institute/Harvard Medical School, Boston, MA, and approved September 26, 2019 (received for review August 2, 2019) PM20D1 is a candidate thermogenic enzyme in mouse fat, with its without UCP1 to stimulate energy expenditure. NAA analogs are expression cold-induced and enriched in brown versus white adipo- thus in the early stages of pharmacological development (8). cytes. Thiazolidinedione (TZD) antidiabetic drugs, which activate the PM20D1 has also emerged from human genetic studies, most peroxisome proliferator-activated receptor-γ (PPARγ) nuclear recep- notably in neurodegenerative diseases.
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ACY1 Rabbit Pab
Leader in Biomolecular Solutions for Life Science ACY1 Rabbit pAb Catalog No.: A6351 Basic Information Background Catalog No. This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the A6351 hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene Observed MW is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung 46kDa cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly Calculated MW homologous to the porcine counterpart, and this enzyme is the first member of a new 37kDa/38kDa/42kDa/45kDa family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and Category increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between Primary antibody this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on Applications chromosome 18. WB, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 95 Q03154 IF 1:10 - 1:100 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1). Synonyms ACY1;ACY-1;ACY1D;HEL-S-5 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃.
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ACY1 Gene Aminoacylase 1
ACY1 gene aminoacylase 1 Normal Function The ACY1 gene provides instructions for making an enzyme called aminoacylase 1, which is found in many tissues and organs, including the kidneys and the brain. This enzyme is involved in the breakdown of proteinswhen they are no longer needed. Many proteins in the body have a chemical group called an acetyl group attached to one end. This modification, called N-acetylation, helps protect and stabilize the protein. Aminoacylase 1 performs the final step in the breakdown of these proteins by removing the acetyl group from certain protein building blocks (amino acids). The amino acids can then be recycled and used to build other proteins. Health Conditions Related to Genetic Changes Aminoacylase 1 deficiency Several mutations in the ACY1 gene have been identified in people with a condition called aminoacylase 1 deficiency. This condition is characterized by delayed development of mental and motor skills and other neurological problems, although some people with the condition have no signs or symptoms. Most of the associated ACY1 gene mutations change single amino acids in the aminoacylase 1 enzyme. These and other ACY1 gene mutations lead to production of an aminoacylase 1 enzyme with little or no function. Without this enzyme's function, acetyl groups are not efficiently removed from a subset of amino acids (including methionine, glutamic acid, alanine, serine, glycine, leucine, valine, threonine, and isoleucine) during the breakdown of proteins. The excess N-acetylated amino acids are released from the body in urine. It is not known how a reduction of aminoacylase 1 function leads to neurological problems in people with aminoacylase 1 deficiency.
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