Physicochemical Properties and Function of Plant Polyphenol Oxidase: a Review'
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Characterization of Polyphenol Oxidase and Antioxidants from Pawpaw (Asimina Tribola) Fruit
University of Kentucky UKnowledge University of Kentucky Master's Theses Graduate School 2007 CHARACTERIZATION OF POLYPHENOL OXIDASE AND ANTIOXIDANTS FROM PAWPAW (ASIMINA TRIBOLA) FRUIT Caodi Fang University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Fang, Caodi, "CHARACTERIZATION OF POLYPHENOL OXIDASE AND ANTIOXIDANTS FROM PAWPAW (ASIMINA TRIBOLA) FRUIT" (2007). University of Kentucky Master's Theses. 477. https://uknowledge.uky.edu/gradschool_theses/477 This Thesis is brought to you for free and open access by the Graduate School at UKnowledge. It has been accepted for inclusion in University of Kentucky Master's Theses by an authorized administrator of UKnowledge. For more information, please contact [email protected]. ABSTRACT OF THESIS CHARACTERIZATION OF POLYPHENOL OXIDASE AND ANTIOXIDANTS FROM PAWPAW (ASIMINA TRIBOLA) FRUIT Crude polyphenol oxidase (PPO) was extracted from pawpaw (Asimina triloba) fruit. The enzyme exhibited a maximum activity at pH 6.5–7.0 and 5–20 °C, and had -1 a maximum catalysis rate (Vmax) of 0.1363 s and a reaction constant (Km) of 0.3266 M. It was almost completely inactivated when incubated at 80 °C for 10 min. Two isoforms of PPO (MW 28.2 and 38.3 kDa) were identified by Sephadex gel filtration chromatography and polyacrylamide gel electrophoresis. Both the concentration and the total activity of the two isoforms differed (P < 0.05) between seven genotypes of pawpaw tested. Thermal stability (92 °C, 1–5 min) and colorimetry (L* a* b*) analyses showed significant variations between genotypes. -
3. Methodology
3. METHODOLOGY The present study focused on the responses evoked by B. monnieri under conditions of oxidative stress. The plan of work and the methodology adopted are presented in this chapter. The work was carried out in four phases. In Phase I, different parts of B. monnieri , namely leaves, stolon and roots, were assessed for their antioxidant potential and free radical scavenging activity. The biomolecular protective effects and the apoptosis- modulating effects of the leaf extract in cell free and in vitro systems were studied in Phase II. Phase III involved assessing the antioxidant status in precision-cut liver slices exposed to oxidative stress and confirming the results in experimental animals. In the final phase of the study, phytochemical analysis of the active component in B. monnieri leaves was carried out. The layout of the study is presented below. PHASE I In this phase, the antioxidant status in different parts of B. monnieri was assessed. The leaves, stolon and roots of the plantlets were collected from the plants grown in pots in the University campus. They were washed thoroughly in running tap water in order to remove any dirt or soil particles adhered and blotted gently between folds of tissue paper to remove any water droplets. Both enzymic and non-enzymic antioxidants were analysed in all the three parts of the plant. ENZYMIC ANTIOXIDANTS The enzymic antioxidants analyzed in the parts of B. monnieri w ere superoxide dismutase, catalase, peroxidase, glutathione S-transferase and polyphenol oxidase. ASSAY OF SUPEROXIDE DISMUTASE (SOD) SOD was assayed according to the method of Kakkar et al. -
Plant Phenolics: Bioavailability As a Key Determinant of Their Potential Health-Promoting Applications
antioxidants Review Plant Phenolics: Bioavailability as a Key Determinant of Their Potential Health-Promoting Applications Patricia Cosme , Ana B. Rodríguez, Javier Espino * and María Garrido * Neuroimmunophysiology and Chrononutrition Research Group, Department of Physiology, Faculty of Science, University of Extremadura, 06006 Badajoz, Spain; [email protected] (P.C.); [email protected] (A.B.R.) * Correspondence: [email protected] (J.E.); [email protected] (M.G.); Tel.: +34-92-428-9796 (J.E. & M.G.) Received: 22 October 2020; Accepted: 7 December 2020; Published: 12 December 2020 Abstract: Phenolic compounds are secondary metabolites widely spread throughout the plant kingdom that can be categorized as flavonoids and non-flavonoids. Interest in phenolic compounds has dramatically increased during the last decade due to their biological effects and promising therapeutic applications. In this review, we discuss the importance of phenolic compounds’ bioavailability to accomplish their physiological functions, and highlight main factors affecting such parameter throughout metabolism of phenolics, from absorption to excretion. Besides, we give an updated overview of the health benefits of phenolic compounds, which are mainly linked to both their direct (e.g., free-radical scavenging ability) and indirect (e.g., by stimulating activity of antioxidant enzymes) antioxidant properties. Such antioxidant actions reportedly help them to prevent chronic and oxidative stress-related disorders such as cancer, cardiovascular and neurodegenerative diseases, among others. Last, we comment on development of cutting-edge delivery systems intended to improve bioavailability and enhance stability of phenolic compounds in the human body. Keywords: antioxidant activity; bioavailability; flavonoids; health benefits; phenolic compounds 1. Introduction Phenolic compounds are secondary metabolites widely spread throughout the plant kingdom with around 8000 different phenolic structures [1]. -
Oxidation in Foods and Beverages and Antioxidant Applications
Oxidation in foods and beverages and antioxidant applications ß Woodhead Publishing Limited, 2010 Related titles: Oxidation in foods and beverages and antioxidant applications Volume 2 Management in different industry sectors (ISBN 978-1-84569-983-3) Oxidative rancidity is a major cause of food quality deterioration, leading to the formation of undesirable off-flavours as well as unhealthy compounds. The two volumes of Oxidation in foods and beverages and antioxidant applications review food quality deterioration due to oxidation and methods for its control. The second volume concentrates on oxidation and its management in different industry sectors. Part I discusses animal products, with chapters on the oxidation and protection of red meat, poultry, fish and dairy products. Part II reviews oxidation in plant-based foods and beverages, including edible oils, wine and fried products. The final chapters examine encapsulation to inhibit lipid oxidation and antioxidant active packaging and edible films. Chemical deterioration and physical instability of foods and beverages (ISBN 978-1-84569-495-1) For a food product to be a success in the marketplace, it must be stable throughout its shelf-life. Changes due to food chemical deterioration and physical instability are not always recognised by food producers, who are more familiar with microbial spoilage, yet can be just as problematic. This book provides an authoritative review of key topics in this area. Chapters in Parts I and II focus on the chemical reactions and physical changes that negatively affect food quality. The remaining chapters outline the likely effects on different food products, for example baked goods, fruit and vegetables and beverages. -
Polyphenol Oxidases in Plants and Fungi: Going Places? a Review
PHYTOCHEMISTRY Phytochemistry 67 (2006) 2318–2331 www.elsevier.com/locate/phytochem Review Polyphenol oxidases in plants and fungi: Going places? A review Alfred M. Mayer Department of Plant and Environmental Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel Received 3 June 2006; received in revised form 22 July 2006 Available online 14 September 2006 Abstract The more recent reports on polyphenol oxidase in plants and fungi are reviewed. The main aspects considered are the structure, dis- tribution, location and properties of polyphenol oxidase (PPO) as well as newly discovered inhibitors of the enzyme. Particular stress is given to the possible function of the enzyme. The cloning and characterization of a large number of PPOs is surveyed. Although the active site of the enzyme is conserved, the amino acid sequence shows very considerable variability among species. Most plants and fungi PPO have multiple forms of PPO. Expression of the genes coding for the enzyme is tissue specific and also developmentally controlled. Many inhibitors of PPO have been described, which belong to very diverse chemical structures; however, their usefulness for controlling PPO activity remains in doubt. The function of PPO still remains enigmatic. In plants the positive correlation between levels of PPO and the resistance to pathogens and herbivores is frequently observed, but convincing proof of a causal relationship, in most cases, still has not been published. Evidence for the induction of PPO in plants, particularly under conditions of stress and pathogen attack is consid- ered, including the role of jasmonate in the induction process. A clear role of PPO in a least two biosynthetic processes has been clearly demonstrated. -
Unravel Browning Mechanism in Making Kue Delapan Jam
International Food Research Journal 24(1): 310-317 (February 2017) Journal homepage: http://www.ifrj.upm.edu.my Unravel browning mechanism in making kue delapan jam 1*Agustini, S., 2Priyanto, G., 2Hamzah, B., 2Santoso, B. and 2Pambayun, R. 1Palembang Institute for Industrial Research and Standardization, Jalan Perindustrian 2 nomor 12 Sukarami Palembang, Indonesia 30152 2Department of Agricultural Technology, College of Agriculture, Sriwijaya University, Jl. Padang Selasa Nomor 524 Bukit Besar Palembang, Indonesia 30139 Article history Abstract Received: 14 September 2015 This research intended to study browning reaction mechanism in making kue delapan jam Received in revised form: and its implication on nutritional value. Browning reaction among substance exist in raw 8 March 2016 material during processing influence the sensories quality and color of cake significantly. Accepted: 14 March 2016 The study was based on the theoretical approaches relate to browning reaction, taking into account the experimental test, material balance, and the available information. The reaction pathway (Maillard reaction, caramelization, lipid oxidation) was determined by the changes Keywords in concentration of raw material (dough) and selected observable chemical marker according to their ability to indentify reaction stages. Research applied completely randomized design Browning with steaming time as a treatment (0 h, and 8 hours) with 3 replications. Reaction pathway Kue delapan jam Reaction mechanism determined by observing changes of sucrose, lactose, glucose, fructose, hidroxy methyl furfural, Steaming acetic acid, formic acid, amino acid and fatty acid between the dough and the cake. Test results showed that there were no significant changes in sucrose concentration. No fructose, glucose, acetic acid, formic acid, and hidroxy methyl furfural indentified both in the dough and the cake. -
Modulation of Lipid Metabolism by Phytosterol Stearates and Black Raspberry Seed Oils
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Nutrition & Health Sciences Dissertations & Theses Nutrition and Health Sciences, Department of 5-2010 Modulation of Lipid Metabolism by Phytosterol Stearates and Black Raspberry Seed Oils Mark McKinley Ash University of Nebraska at Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/nutritiondiss Part of the Dietetics and Clinical Nutrition Commons, and the Molecular, Genetic, and Biochemical Nutrition Commons Ash, Mark McKinley, "Modulation of Lipid Metabolism by Phytosterol Stearates and Black Raspberry Seed Oils" (2010). Nutrition & Health Sciences Dissertations & Theses. 17. https://digitalcommons.unl.edu/nutritiondiss/17 This Article is brought to you for free and open access by the Nutrition and Health Sciences, Department of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Nutrition & Health Sciences Dissertations & Theses by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Modulation of Lipid Metabolism by Phytosterol Stearates and Black Raspberry Seed Oils by Mark McKinley Ash A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Master of Science Major: Nutrition Under the Supervision of Professor Timothy P. Carr Lincoln, Nebraska May, 2010 Modulation of Lipid Metabolism by Phytosterol Stearates and Black Raspberry Seed Oils Mark McKinley Ash, M.S. University of Nebraska, 2010 Adviser: Timothy P. Carr Naturally occurring compounds and lifestyle modifications as combination and mono- therapy are increasingly used for dyslipidemia. Specficially, phytosterols and fatty acids have demonstrated an ability to modulate cholesterol and triglyceride metabolism in different fashions. -
Phenolics in Human Health
International Journal of Chemical Engineering and Applications, Vol. 5, No. 5, October 2014 Phenolics in Human Health T. Ozcan, A. Akpinar-Bayizit, L. Yilmaz-Ersan, and B. Delikanli with proteins. The high antioxidant capacity makes Abstract—Recent research focuses on health benefits of polyphenols as an important key factor which is involved in phytochemicals, especially antioxidant and antimicrobial the chemical defense of plants against pathogens and properties of phenolic compounds, which is known to exert predators and in plant-plant interferences [9]. preventive activity against infectious and degenerative diseases, inflammation and allergies via antioxidant, antimicrobial and proteins/enzymes neutralization/modulation mechanisms. Phenolic compounds are reactive metabolites in a wide range of plant-derived foods and mainly divided in four groups: phenolic acids, flavonoids, stilbenes and tannins. They work as terminators of free radicals and chelators of metal ions that are capable of catalyzing lipid oxidation. Therefore, this review examines the functional properties of phenolics. Index Terms—Health, functional, phenolic compounds. I. INTRODUCTION In recent years, fruits and vegetables receive considerable interest depending on type, number, and mode of action of the different components, so called as “phytochemicals”, for their presumed role in the prevention of various chronic diseases including cancers and cardiovascular diseases. Plants are rich sources of functional dietary micronutrients, fibers and phytochemicals, such -
The Role of Lipids in Nonenzymatic Browning
Grasas y Aceites 35 Vol. 51. Fasc. 1-2 (2000), 35-49 The role of lipids in nonenzymatic browning By Francisco J. Hidalgo and Rosario Zamora Instituto de la Grasa, CSIC, Avenida Padre García Tejero 4, 41012 Sevilla, Spain RESUMEN 1. INTRODUCTION El papel de los lípidos en el pardeamiento no enzimático When we select foods and when we eat, we En este trabajo se hace una revisión del papel de los lípidos en use all of our physical senses, including sight, el pardeamiento no enzimático de alimentos mediante el estudio de touch, smell, taste, and even hearing. These las reacciones proteína/lípido oxidado en comparación con otras senses allow us to determine food quality, which reacciones donde ocurre también este oscurecimiento: la reacción de Maillard, el pardeamiento producido por el ácido ascórbico, y las can be divided into three main categories: reacciones de las quinonas con los grupos amino. Los appearance, textural and flavor factors (Francis, mecanismos propuestos para estas reacciones de producción de 1999; Ghorpade et al. 1995; Potter and Hotchkiss, color y fluorescencia, así como la formación de melanoidinas, 1995). Appearance factors include such things as lipofuscinas y productos coloreados de bajo peso molecular son size, shape, wholeness, different forms of discutidos de forma comparada, concluyendo que el papel de los lípidos en estas reacciones no parece ser muy diferente del papel damage, gloss, transparency, color, and de los carbohidratos en el Maillard o de los fenoles en el par- consistency. Textural factors include handfeel and deamiento enzimático. Estas reacciones carbonil-amino parecen mouthfeel of firmness, softness, juiciness, ser un grupo de reacciones secundarias que ocurren de forma chewiness, grittiness. -
Comparison of Antioxidant Activities and Total Polyphenolic and Methylxanthine Contents Between the Unripe Fruit and Leaves of Ilex Paraguariensis A
ORIGINAL ARTICLES Universidade Regional Integrada do Alto Uruguai e das Misso˜es1, Programa de Po´s-Graduac¸a˜o em Cieˆncias Farmaceˆuti- cas2, Curso de Farma´cia3, Departamento de Microbiologia4, Departamento de Farma´cia Industrial5, Universidade Federal de Santa Maria, Campus Camobi, Santa Maria, Rio Grande do Sul, Brasil Comparison of antioxidant activities and total polyphenolic and methylxanthine contents between the unripe fruit and leaves of Ilex paraguariensis A. St. Hil. A. Schubert1, D. F. Pereira2, F. F. Zanin3, S. H. Alves4, R. C. R. Beck5, M. L. Athayde5 Received February 15, 2007, accepted March 8, 2007 Prof. Dr. Margareth Linde Athayde, Departamento de Farma´cia Industrial, Pre´dio 26, sala 1115, Campus Camobi, Universidade Federal de Santa Maria, RS, Brasil. CEP 97105-900 [email protected] Pharmazie 62: 876–880 (2007) doi: 10.1691/ph.2007.11.7052 Ilex paraguariensis is used in Brazil as a stimulating beverage called “mate”. Leaves and immature fruit extracts of Ilex paraguariensis were evaluated for their radical scavenging capacity, total methyl- xanthine and polyphenol contents. Antimicrobial activity of two enriched saponin fractions obtained from the fruits were also evaluated. The radical scavenging activity of the fractioned extracts was determined spectrophotometrically using 1,1-diphenylpicrylhydrazyl free radical (DPPH). The IC50 of l-ascorbic acid, ethyl acetate and n-butanol fractions from the leaves and ethyl acetate fraction from the fruits were 6.48 mg/mL, 13.26 mg/mL, 27.22 mg/mL, and 285.78 mg/mL, respectively. Total methylxanthine content was 1.16 Æ 0.06 mg/g dry weight in the fruits and 8.78 Æ 0.01 mg/g in the leaves. -
Relevance of Physical Properties in the Stability of Plant-Based Food Products
Indian Journal of Experimental Biology Vol. 51, November 2013, pp. 895-904 Review Article Relevance of physical properties in the stability of plant-based food products Elena Venir* & Enrico Maltini Department of Food Science, University of Udine, Via Sondrio 2/A, 33100 Udine, Italy Plant tissues are composed of a watery solution of low molecular weight species, mainly sugars, salts and organic acids, and of high molecular weight hydrocolloids, contained in a water insoluble matrix of macromolecules, mostly carbohydrates. All these constituents interact with water, thus reducing its thermodynamic vapour pressure (aw), with small molecules interacting through polar binding, and large biopolymers through surface and capillary effects. Similarly, some constituents will greatly affect kinetic glass transition temperatures (Tg), while others will not. As regards stability, while microbial and chemical changes are mainly related to aw, structure-related changes such as collapse are dependent on the glass transition temperature, Tg. In simple systems such as juices, both thermodynamic and kinetic approaches, employed respectively for high and low moisture systems, have predictive ability, which can be unified in the concept of “critical aw”. However, in complex, multidomain, multiphase systems, such as vegetables and fruits, where insoluble polymeric phases are present, hydrocolloids such as soluble pectins will only slightly affect Tg and aw, but significantly increase the macro viscosity of the soluble fraction, thereby reducing the tendency to collapse. In such cases the use of Tg as a predictive tool must be considered with care. The interrelationships among these aspects are discussed in detail below. Keywords: Glass transition, Plant food, Stability, Water activity Food stability is influenced by chemical, physical and hydrocolloids4. -
Identification of the 100 Richest Dietary Sources of Polyphenols: an Application of the Phenol-Explorer Database
European Journal of Clinical Nutrition (2010) 64, S112–S120 & 2010 Macmillan Publishers Limited All rights reserved 0954-3007/10 www.nature.com/ejcn ORIGINAL ARTICLE Identification of the 100 richest dietary sources of polyphenols: an application of the Phenol-Explorer database JPe´rez-Jime´nez1,2, V Neveu1,2,FVos1,2 and A Scalbert1,2 1Clermont Universite´, Universite´ d’Auvergne, Unite´ de Nutrition Humaine, Saint-Genes-Champanelle, France and 2INRA, UMR 1019, UNH, CRNH Auvergne, Saint-Genes-Champanelle, France Background/Objectives: The diversity of the chemical structures of dietary polyphenols makes it difficult to estimate their total content in foods, and also to understand the role of polyphenols in health and the prevention of diseases. Global redox colorimetric assays have commonly been used to estimate the total polyphenol content in foods. However, these assays lack specificity. Contents of individual polyphenols have been determined by chromatography. These data, scattered in several hundred publications, have been compiled in the Phenol-Explorer database. The aim of this paper is to identify the 100 richest dietary sources of polyphenols using this database. Subjects/Methods: Advanced queries in the Phenol-Explorer database (www.phenol-explorer.eu) allowed retrieval of information on the content of 502 polyphenol glycosides, esters and aglycones in 452 foods. Total polyphenol content was calculated as the sum of the contents of all individual polyphenols. These content values were compared with the content of antioxidants estimated using the Folin assay method in the same foods. These values were also extracted from the same database. Amounts per serving were calculated using common serving sizes.