PADI2 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science PADI2 Rabbit pAb Catalog No.: A11711 Basic Information Background Catalog No. This gene encodes a member of the peptidyl arginine deiminase family of enzymes, A11711 which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have Observed MW distinct substrate specificities and tissue-specific expression patterns. The type II enzyme 75kDa is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and Calculated MW macrophages. This enzyme is thought to play a role in the onset and progression of 49kDa/75kDa neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in Category a cluster with four other paralogous genes. Primary antibody Applications WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 11240 Q9Y2J8 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 26-210 of human PADI2 (NP_031391.2). Synonyms PADI2;PAD-H19;PAD2;PDI2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PADI2 antibody (A11711) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Down-Regulation of PADI2 Prevents Proliferation and Epithelial
Liu et al. J Transl Med (2020) 18:357 https://doi.org/10.1186/s12967-020-02528-0 Journal of Translational Medicine RESEARCH Open Access Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib Lidong Liu1,2,3, Zhiwei Zhang1, Guoxiang Zhang1, Ting Wang1, Yingchun Ma1 and Wei Guo1* Abstract Background: Epithelial ovarian cancer (EOC) is the most lethal disease among female genital malignant tumors. Peptidylarginine deiminase type II(PADI II) has been shown to enhance a variety of cancers carcinogenesis, including ovarian cancer. The purpose of this study was to investigate the biological role of PADI2 in ovarian cancer (OC) and the relative mechanism. Methods: Gene Expression Profling Interactive Analysis (GEPIA) (https ://gepia .pku.cn/) and ONCOMINE (https :// www.oncom ine.org/) were used to analyze PADI2 Gene Expression data. The survival curve for the PADI2 gene was generated by using the online Kaplan–Meier mapping site (https ://www.kmplo t.com/). We conducted MTT assay, cloning formation assay and EdU cell proliferation assay to detect the cell activity of PADI2 knockdown A2780 and SKOV3 ovarian cancer cells treated with Olaparib. Cell migration and invasion were observed by would healing and transwell assay. The pathway changes after the treatment of PADI2 were detected by transcriptome sequencing and western blot. The role of PADI2 combined with Olaparib treatment in vivo was studied in nude mouse model bearing ovarian cancer tumor. Results: We investigated the role of PADI2 on EOC in vitro and in vivo.
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Control Association Study of PADI2 with Schizophrenia
Journal of Human Genetics (2009) 54, 430–432 & 2009 The Japan Society of Human Genetics All rights reserved 1434-5161/09 $32.00 www.nature.com/jhg SHORT COMMUNICATION A two-stage case–control association study of PADI2 with schizophrenia Yuichiro Watanabe1,2, Ayako Nunokawa1, Naoshi Kaneko1, Tadao Arinami3, Hiroshi Ujike4, Toshiya Inada5, Nakao Iwata6, Hiroshi Kunugi7, Masanari Itokawa8, Takeshi Otowa9, Norio Ozaki10 and Toshiyuki Someya1 Peptidylarginine deiminases (PADIs), five isoforms of which have been identified, catalyze the conversion of arginine residues to citrulline residues in proteins. Recent studies have revealed that abnormal activation of PADI2, the gene for which is expressed throughout the nervous system, is likely to be related to the pathogenesis of neuropsychiatric diseases with neurodegenerative processes, such as Alzheimer’s disease and multiple sclerosis. Such a progressive neurodegenerative process could be involved in the etiology and/or course of schizophrenia, and PADI2 may be a candidate gene for schizophrenia. To assess whether PADI2 has a role in vulnerability to schizophrenia, we conducted a two-stage case–control association study in Japanese individuals. In a screening population of 534 patients and 559 control individuals, we examined eight single-nucleotide polymorphisms (SNPs) including four haplotype tag SNPs and four coding SNPs in PADI2. There was a potential association of a synonymous SNP in exon 7 with schizophrenia. However, we could not replicate this association in a confirmatory population of 2126 patients and 2228 control individuals. The results of this study suggest that PADI2 does not contribute to genetic susceptibility to schizophrenia. Journal of Human Genetics (2009) 54, 430–432; doi:10.1038/jhg.2009.52; published online 29 May 2009 Keywords: case–control study; Japanese; PADI2; schizophrenia; single-nucleotide polymorphism Schizophrenia is a complex genetic disorder that affects approximately performed a two-stage case–control association study in Japanese 1% of the population worldwide.
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Deimination, Intermediate Filaments and Associated Proteins
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E002549.Full.Pdf
Open access Original research J Immunother Cancer: first published as 10.1136/jitc-2021-002549 on 10 June 2021. Downloaded from Protein citrullination as a source of cancer neoantigens 1 2 3 Hiroyuki Katayama , Makoto Kobayashi, Ehsan Irajizad, 1 1 1 1 Alejandro M Sevillano , Nikul Patel, Xiangying Mao, Leona Rusling, Jody Vykoukal,1 Yining Cai,1 Fuchung Hsiao,1 Chuan- Yih Yu,1 James Long,3 4 5 1 1 Jinsong Liu, Franscisco Esteva, Johannes Fahrmann, Sam Hanash To cite: Katayama H, ABSTRACT modification of proteins via the deamination Kobayashi M, Irajizad E, et al. Background Citrulline post- translational modification of arginine to citrulline. In total, five PADI Protein citrullination as a source of proteins is mediated by protein arginine deiminase of cancer neoantigens. Journal family members are known with sequence (PADI) family members and has been associated with 2 for ImmunoTherapy of Cancer homology ranging from 70% to 95%. To autoimmune diseases. The role of PADI- citrullinome in 2021;9:e002549. doi:10.1136/ date no enzyme has been identified that can jitc-2021-002549 immune response in cancer has not been evaluated. We hypothesized that PADI- mediated citrullinome is a source reverse protein citrullination. The role of of neoantigens in cancer that induces immune response. protein citrullination has been best investi- ► Additional supplemental gated in the context of rheumatoid arthritis material is published online only. Methods Protein expression of PADI family members was To view, please visit the journal evaluated in 196 cancer cell lines by means of indepth (RA), where elevated protein citrullination, online (http:// dx.
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