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Colicin M for the Control of Ehec Colicin M Zur Kontrolle Von Ehec Colicine M Pour Le Contrôle De Ehec

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Colicin M for the Control of Ehec Colicin M Zur Kontrolle Von Ehec Colicine M Pour Le Contrôle De Ehec (19) *EP003097783B1* (11) EP 3 097 783 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: A01N 63/02 (2006.01) A01P 1/00 (2006.01) 13.11.2019 Bulletin 2019/46 (21) Application number: 15181133.8 (22) Date of filing: 14.08.2015 (54) COLICIN M FOR THE CONTROL OF EHEC COLICIN M ZUR KONTROLLE VON EHEC COLICINE M POUR LE CONTRÔLE DE EHEC (84) Designated Contracting States: • L.SARADA NANDIWADA ET AL: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB "Characterization of an E2-type colicin and its GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO application to treat alfalfa seeds to reduce PL PT RO RS SE SI SK SM TR Escherichia coli O157:H7", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol. 93, no. (30) Priority: 26.05.2015 US 201562166379 P 3, 1 June 2004 (2004-06-01), pages 267-279, XP055226209, NL ISSN: 0168-1605, DOI: (43) Date of publication of application: 10.1016/j.ijfoodmicro.2003.11.009 30.11.2016 Bulletin 2016/48 • H. TOSHIMA ET AL: "Enhancement of Shiga Toxin Production in Enterohemorrhagic (73) Proprietor: Nomad Bioscience GmbH Escherichia coli Serotype O157:H7 by DNase 80333 München (DE) Colicins", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 23, 1 December 2007 (72) Inventors: (2007-12-01), pages 7582-7588, XP055226172, US • GIRITCH, Anatoli ISSN: 0099-2240, DOI: 10.1128/AEM.01326-07 06108 Halle (DE) • DATABASE MEDLINE [Online] US NATIONAL • HAHN, Simone LIBRARY OF MEDICINE (NLM), BETHESDA, MD, 06217 Merseburg (DE) US; 2011, ETCHEVERRÍA A I ET AL: "Reduction • SCHULZ, Steve of Adherence of E. coli O157:H7 to HEp-2 Cells 06114 Halle (DE) and to Bovine Large Intestinal Mucosal Explants • STEPHAN, Anett by Colicinogenic E. coli.", XP002750605, 06114 Halle (DE) Database accession no. NLM23724308 & ISRN • GLEBA, Yuri MICROBIOLOGY 2011, vol. 2011, 2011, page 10117 Berlin (DE) 697020, ISSN: 2090-7478 • JARCZOWSKI, Franziska • DATABASE MEDLINE [Online] US NATIONAL 06317 Seegebiet ML (DE) LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; March 2004 (2004-03), SCHAMBERGER (74) Representative: Blodig, Wolfgang GERRY P ET AL: "Characterization of Wächtershäuser & Hartz colicinogenic Escherichia coli strains inhibitory Patentanwaltspartnerschaft mbB to enterohemorrhagic Escherichia coli.", Weinstrasse 8 XP002750606, Database accession no. 80333 München (DE) NLM15035362 & JOURNAL OF FOOD PROTECTION MAR 2004, vol. 67, no. 3, March (56) References cited: 2004 (2004-03), pages 486-492, ISSN: 0362-028X WO-A1-2014/009744 WO-A1-2015/121443 WO-A2-2005/089812 US-A1- 2015 050 253 Note: Within nine months of the publication of the mention of the grant of the European patent in the European Patent Bulletin, any person may give notice to the European Patent Office of opposition to that patent, in accordance with the Implementing Regulations. Notice of opposition shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention). EP 3 097 783 B1 Printed by Jouve, 75001 PARIS (FR) (Cont. next page) EP 3 097 783 B1 • M. EL GHACHI ET AL: "Colicin M Exerts Its • VOLKMAR BRAUN ET AL: "Ton-dependent Bacteriolytic Effect via Enzymatic Degradation of colicins and microcins: modular design and Undecaprenyl Phosphate-linked Peptidoglycan evolution", BIOCHIMIE, vol. 84, no. 5-6, 1 May Precursors", JOURNAL OF BIOLOGICAL 2002 (2002-05-01), pages 365-380, XP055226181, CHEMISTRY, vol. 281, no. 32, 11 August 2006 FR ISSN: 0300-9084, DOI: (2006-08-11), pages 22761-22772, XP055226165, 10.1016/S0300-9084(02)01427-X US ISSN: 0021-9258, DOI: 10.1074/jbc.M602834200 2 EP 3 097 783 B1 Description FIELD OF THE INVENTION 5 [0001] The invention provides a use of colicin M or a derivative thereof for preventing or reducing contamination of food and other objects with EHEC. BACKGROUND OF THE INVENTION 10 [0002] Escherichia coli is a normal inhabitant of human gastro-intestinal (GI) tract, however, some E. coli strains are pathogenic (EPEC = enteropathogenic E. coli). Enterohaemorrhagic E. coli (EHEC) target the small and large bowels and can cause haemorrhagic colitis and HUS (haemolytic uraemic syndrome). STEC is an abbreviation for Shiga-toxin producing E. coli. EHEC strains are STEC strains. Shiga-toxin (Stx) acts on the lining of the blood vessels and the vascular endothelium. The toxin penetrates into endothelial cells. When inside the cell, Stx inactivate protein synthesis 15 leading to the death of the cell. The vascular endothelium has to continually renew itself, so this killing of cells leads to a breakdown of the lining and to hemorrhage. The first response is commonly a bloody diarrhea. [0003] The toxin is effective against small blood vessels, such as found in the digestive tract, the kidney, and lungs, but not against large vessels such as the arteries or major veins. A specific target for the toxin appears to be the vascular endothelium of the glomerulus in the kidneys. Destroying these structures leads to kidney failure and the development 20 of the often deadly and frequently debilitating HUS. Food poisoning with Shiga toxin often also has effects on the lungs and the nervous system. [0004] A large number of serotypes of STEC isolated from humans is known. From studies in the USA and Canada examining human STEC infections, 50-80% were identified as being caused by E. coli O157:H7. 30 to 50% are caused by non-O157 STEC. Although E. coli O157:H7 has been most commonly identified 25 as the cause of STEC infection, isolation of non-0157 STEC strains from clinical cases, outbreaks and environmental sources has been increasing (Posse et al., FEMS Microbiol Lett. 2008; 282(1):124-31; Posse et al., J. Appl. Microbiol. 2008; 105(1):227-35). A study at the Center for Disease Control and Prevention showed that from 1983-2002 approxi- mately 70% of non-O157 STEC infections in the United States were caused by strains from one of six major serogroups, namely O26, O45, 0103, 0111, 0121 and 0145 (Brooks et al., 2005). Virulence factors for non-O157 STEC include, but 30 are not limited to, production of the shiga-like toxins 1 and/or 2 (Stx1, Stx2) and intimin (eae). USDA-FSIS (United States Department of Agriculture (USDA) and Food Safety and Inspection Service (FSIS)) defined the so-called "Big 7" STEC panel: 0157 and O26, O45, O103, O111, O121 and O145 serotypes. These are considered as most dangerous STEC serotypes ("adulterants"). EHEC serotypes are generally classified using the O antigen which is a part of the lipopoly- saccharide layer, and the H antigen that is flagellin. 35 [0005] Prevention of EHEC or reducing contamination of food with EHEC requires, according to the WHO, control measures at all stages of the food chain, from agricultural production on the farm to processing, manufacturing and preparation of foods in both commercial establishments and household kitchens. As to industry, the WHO recommends that the number of cases of disease might be reduced by various mitigation strategies for ground beef (for example, screening the animals pre-slaughter to reduce the introduction of large numbers of pathogens in the slaughtering envi- 40 ronment). Good hygienic slaughtering practices reduce contamination of carcasses by faeces, but do not guarantee the absence of EHEC from products. Education in hygienic handling of foods for workers at farms, abattoirs and those involved in the food production is essential to keep microbiological contamination to a minimum. So far, the only effective method of eliminating EHEC from foods is to introduce a bactericidal treatment, such as heating (e.g. cooking, pasteur- ization) or irradiation (see: http://www.who.int/mediacentre/factsheets/fs125/en/). 45 [0006] Treatment of EHEC infections in humans is difficult. A multitargeted approach is generally recommended including general supportive measures, anti-platelet and thrombolytic agents and thrombin inhibitor, selective use of antimicrobials, probiotics, toxin neutralizers and antibodies against key pathogenic pathway elements (Goldwater et al., BMC Medicine 2012, 10:12). [0007] Most of the above mentioned methods of preventing EHEC or reducing contamination with EHEC are methods 50 that are essentially independent from a particular pathogenic bacterium or from a particular serotype of EHEC. This has the advantage that little prior knowledge of the specific EHEC serotype in question is necessary before counter-measures are taken. However, the above mentioned methods of preventing EHEC or reducing contamination with EHEC such as heating or irradiation are not always applicable or change the treated good or food in undesirable ways. Other methods may have turned out non-effective with a particular patient. There is therefore a need for further ways of preventing or 55 treating EHEC infections or methods for reducing or preventing contamination of objects with EHEC. [0008] Schamberger and Diez-Gonzalez, Journal of Food Protection, 67 (2004) 486-492 describe the characterization of colicinogenic E. coli strains that are inhibitory to EHEC. The use of colicinogenic E. coli strains for inhibiting O157:H7 in cattle is suggested. 3 EP 3 097 783 B1 [0009] It is an object to provide a use for preventing or reducing contamination of objects, notably, food with EHEC. It is a further object to provide a use for reducing contamination of objects with EHEC, that are effective against a wide range of EHEC serogroups such as the Big 7 or Big 6 groups of serotypes. 5 SUMMARY OF THE INVENTION [0010] This problem has been solved according to the claims. [0011] The inventors have surprisingly found a colicin having at the same time high activity against several EHEC serotypes and an exceptionally low specificity for particular EHEC strains or serotypes, i.e.
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