DOCSLIB.ORG

Haasken Et Al. Macrophage Scavenger Receptor 1 (Msr1, SR-A

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Haasken Et Al. Macrophage Scavenger Receptor 1 (Msr1, SR-A Haasken et al. Macrophage scavenger receptor 1 (Msr1, SR-A) influences B cell autoimmunity by regulating soluble autoantigen concentration Supplemental Figure 1: Decreased incidence and severity of arthritis and endocarditis in Msr1–/– K/BxN mice. (A) Histological analysis of ankle (left panels) and mitral valve (right panels) pathology in Msr1+/+, +/–, and –/– K/BxN mice demonstrates protection from both joint and cardiac valve pathology in non-arthritic Msr1–/– K/BxN mice. For ankles, original magnification = 10x, bar (in fourth row) indicates 200 microns. For mitral valves, original magnification = 40x; bar (in fourth row) indicates 100 microns. (B) Five non-arthritic Msr1–/– K/BxN mice (filled circles) were aged up to 15 weeks, during which any mild arthritis resolved or remained equivalent to the 8-week time point, as demonstrated by changes in ankle thickness. The data from the Msr1+/+ K/BxN mice (reproduced from Figure 1B) are shown for comparison. Plotted values are means ± SEM. *p 0.05, **p 0.01. Supplemental Figure 2: Msr1-sufficient host environment permits Msr1-deficient K/BxN hematopoietic compartment to drive arthritis and B cell activation Rag1–/– recipient mice were sublethally irradiated (300 Rad) and transplanted with 10x106 bone marrow cells from Msr1+/+ K/BxN mice or Msr1–/– K/BxN mice as indicated. (A) The development of arthritis in both groups was determined by weekly arthritis scoring and ankle measurements. Values shown are means +/- SEM. n= 3 mice/group. (B) B cells in the reconstituted mice were analyzed for their ability to bind to GPI-tetramer, for having undergone isotype switching express IgG1 intracellularly, and transitioning to a CD38low GL7+ activated phenotype. Numbers indicate the percentage of cells in each quadrant or gate. (C) Representative flow cytometric plot of CD3– F4/80+ macrophages in the reconstituted mice analyzed for expression of the congenic markers differentiating the donor cells (CD45.1+ CD45.2+) from the host cells (CD45.1– CD45.2+). The numbers shown are percentages. In analysis of multiple mice, the percent of macrophages that were host-derived ranged from 38- 63%. (D) Similar analysis for CD3+ T cells and B220+ B cells reveals that they are entirely donor-derived. Haasken et al. Supplemental Figure 1 A ankle mitral valve KRN negative Msr1 +/+ K/BxN Msr1 +/– K/BxN Msr1–/– K/BxN (arthritic) Msr1–/– K/BxN (non-arthritic) B 1.8 ** ** K/BxN 1.6 ** Msr1+/+ 1.4 Msr1–/– * 1.2 1.0 0.8 0.6 Ankle Width (mm) Ankle Width 0.4 Δ 0.2 0 3691215 Age of mice (weeks) Haasken et al. Supplmental Figure 2 A 12 donor marrow 1.30 10 Msr1+/+ K/BxN 1.10 Msr1–/– K/BxN 8 0.90 6 0.70 0.50 4 Arthritis Score 0.30 2 (mm) Ankle Width Δ 0.10 0 0123456 0123456 Time after transplantation (weeks) Time after transplantation (weeks) B gated B220+ gated GPI+ Gate 1 Gate 2 donor 62 0.42 7.02 0.21 G1 marrow 8.93 Msr1 G2 +/+ G2 K/BxN 0.19 88.7 35.3 2.3 86.9 5.8 59.6 5.66 5.75 0.7 G1 16.5 Msr1 G2 –/– G2 K/BxN 0.13 78.2 decoy tetramer IgM CD38 31.4 3.46 CD38 81.1 12.5 GPI tetramer intracell. IgG1 GL7 CD donor 99.9 CD45.1 0.042 donor host 42.1 CD45.2 CD3 CD3 CD45.1 54.8 host donor 99.7 F4/80 CD45.2 B220 CD45.1 0.21 host CD45.2.
Load more

Recommended publications
  • Tolerance and M2 (Alternative) Macrophage Polarization Are Related Processes Orchestrated by P50 Nuclear Factor ␬B
    Tolerance and M2 (alternative) macrophage polarization are related processes orchestrated by p50 nuclear factor ␬B Chiara Portaa,b, Monica Rimoldic, Geert Raesd, Lea Brysd, Pietro Ghezzie, Diana Di Libertof, Francesco Dielif, Serena Ghislettig, Gioacchino Natolig, Patrick De Baetselierd, Alberto Mantovanic,h, and Antonio Sicaa,b,1 aFondazione Humanitas per la Ricerca, 20089 Rozzano, Italy; bDipartimento di Scienze Chimiche, Alimentari, Farmaceutiche e Farmacologiche, University of Piemonte Orientale A. Avogadro, 28100 Novara, Italy; cIstituto Clinico Humanitas, Istituto di Ricovero e Cura a Carattere Scientifico, 20089 Rozzano, Italy; dLaboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, and Department of Molecular ad Cellular Interactions, 1050 Brussels, Belgium; eBrighton and Sussex Medical School, Brighton BN1 9PX, United Kingdom; fDipartimento di Biopatologia e Metodologie Biochimediche, Universita`di Palermo, 90134 Palermo, Italy; gDepartment of Experimental Oncology, European Institute of Oncology at IFOM-IEO Campus, 20139 Milan, Italy; and hUniversity of Milan, 20133 Milan, Italy Edited by Michael Karin, University of California, San Diego School of Medicine, La Jolla, CA, and approved July 22, 2009 (received for review October 6, 2008) Cells of the monocyte–macrophage lineage play a central role in the regulators (9). For instance, p50 and p52 homodimers act as orchestration and resolution of inflammation. Plasticity is a hallmark repressors because these proteins lack a transcription activation of mononuclear phagocytes, and in response to environmental sig- domain, present in RelA, RelB, v-Rel, and c-Rel (7). Accumulation nals these cells undergo different forms of polarized activation, the of p50 homodimers has been observed in endotoxin-tolerant mac- extremes of which are called classic or M1 and alternative or M2.
    [Show full text]
  • Triggering MSR1 Promotes JNK‐Mediated Inflammation in IL‐4
    Published online: April 26, 2019 Article Triggering MSR1 promotes JNK-mediated inflammation in IL-4-activated macrophages Manman Guo1,†,‡ , Anetta Härtlova1,2,3,4,†,* , Marek Gierlinski 5, Alan Prescott6, Josep Castellvi7, Javier Hernandez Losa7,8, Sine K Petersen3,4, Ulf A Wenzel3,4, Brian D Dill1, Christoph H Emmerich1, Santiago Ramon Y Cajal7,8, David G Russell9 & Matthias Trost1,2,** Abstract Introduction Alternatively activated M2 macrophages play an important role in Phagocytosis is a highly conserved process essential for host maintenance of tissue homeostasis by scavenging dead cells, cell defence and tissue remodelling. It involves the recognition of parti- debris and lipoprotein aggregates via phagocytosis. Using proteo- cles by a variety of cell surface receptors, followed by cargo process- mics, we investigated how alternative activation, driven by IL-4, ing and delivery to lysosomes via phagosome–lysosome fusion, modulated the phagosomal proteome to control macrophage process known as phagosome maturation. This leads to gradual function. Our data indicate that alternative activation enhances acidification of the phagosomal lumen and acquisition of digestive homeostatic functions such as proteolysis, lipolysis and nutrient enzymes required for the degradation of phagosomal cargo. There- transport. Intriguingly, we identified the enhanced recruitment of fore, phagocytosis is not only responsible for elimination of bacterial the TAK1/MKK7/JNK signalling complex to phagosomes of IL-4-acti- pathogens, but also responsible for the clearance of apoptotic cells, vated macrophages. The recruitment of this signalling complex was cell debris and senescence cells and orchestrates the subsequent mediated through K63 polyubiquitylation of the macrophage scav- immune response (Rothlin et al, 2007; Murray & Wynn, 2011; enger receptor 1 (MSR1).
    [Show full text]
  • In This Issue
    In This Issue J Immunol 2013; 191:985-986; ; doi: 10.4049/jimmunol.1390039 This information is current as http://www.jimmunol.org/content/191/3/985 of September 29, 2021. Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2013/07/19/191.3.985.DC1 Material Why The JI? Submit online. http://www.jimmunol.org/ • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on September 29, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2013 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Th eJournal of In Th is Issue Immunology Driving DC Change IL-18 by human monocytes, and subsequent induction of the gd T cell chemokine CCL2. Thus after an initial activation of gd oth Th1 and Th17 cells are T cells, zoledronate toxicity inhibited gd T cell expansion, an B involved in the pathogene- unwanted “brake” that could be reversed by the addition of sis of experimental auto- GGPP. The authors determined that exogenous IL-18 elim- immune encephalomyelitis (EAE), inated the need for accessory cell costimulation and, with and it has been found that Th17 cells GGPP, enhanced the ability of zoledronate to generate ef- can convert to the Th1 phenotype fective gd T cells.
    [Show full text]
  • Aging and Sex: Impact on Microglia Phagocytosis
    Received: 7 April 2020 | Revised: 23 May 2020 | Accepted: 6 June 2020 DOI: 10.1111/acel.13182 ORIGINAL ARTICLE Aging and sex: Impact on microglia phagocytosis Natalia Yanguas-Casás1,2 | Andrea Crespo-Castrillo1 | Maria-Angeles Arevalo1,2 | Luis Miguel Garcia-Segura1,2 1Consejo Superior de Investigaciones Científicas (CSIC), Instituto Cajal, Madrid, Abstract Spain Microglia dysfunction and activation are important hallmarks of the aging brain 2 Centro de Investigación Biomédica en Red and are concomitant with age-related neurodegeneration and cognitive decline. de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, Age-associated changes in microglia migration and phagocytic capacity result in Madrid, Spain maladaptive responses, chronic neuroinflammation, and worsened outcomes in Correspondence neurodegenerative disorders. Given the sex bias in the incidence, prevalence, and Natalia Yanguas-Casás, Centro de therapy response of most neurological disorders, we have here examined whether Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable the phagocytic activity of aged microglia is different in males and females. With (CIBERFES), Instituto de Salud Carlos III, this aim, the phagocytosis activity of male and female cells was compared in an in Madrid, Spain. Email: [email protected] vitro aged microglia model and in microglia isolated from adult (5-month-old) or aged (18-month-old) mice. In both models, the phagocytosis of neural debris increased Present address Natalia Yanguas-Casás, IIS Puerta de Hierro- with aging in male and female cells and was higher in aged female microglia than in Segovia de Arana (IDIPHISA), Majadahonda, aged male cells. However, female aged microglia lost its ability to adapt its phagocytic Spain activity to inflammatory conditions.
    [Show full text]
  • Cx3cr1 Mediates the Development of Monocyte-Derived Dendritic Cells During Hepatic Inflammation
    CX3CR1 MEDIATES THE DEVELOPMENT OF MONOCYTE-DERIVED DENDRITIC CELLS DURING HEPATIC INFLAMMATION. Supplementary material Supplementary Figure 1: Liver CD45+ myeloid cells were pre-gated for Ly6G negative cells for excluding granulocytes and HDCs subsequently analyzed among the cells that were CD11c+ and had high expression of MHCII. Supplementary Table 1 low/- high + Changes in gene expression between CX3CR1 and CX3CR1 CD11b myeloid hepatic dendritic cells (HDCs) from CCl4-treated mice high Genes up-regulated in CX3CR1 HDCs Gene Fold changes P value Full name App 4,01702 5,89E-05 amyloid beta (A4) precursor protein C1qa 9,75881 1,69E-22 complement component 1, q subcomponent, alpha polypeptide C1qb 9,19882 3,62E-20 complement component 1, q subcomponent, beta polypeptide Ccl12 2,51899 0,011769 chemokine (C-C motif) ligand 12 Ccl2 6,53486 6,37E-11 chemokine (C-C motif) ligand 2 Ccl3 4,99649 5,84E-07 chemokine (C-C motif) ligand 3 Ccl4 4,42552 9,62E-06 chemokine (C-C motif) ligand 4 Ccl6 3,9311 8,46E-05 chemokine (C-C motif) ligand 6 Ccl7 2,60184 0,009272 chemokine (C-C motif) ligand 7 Ccl9 4,17294 3,01E-05 chemokine (C-C motif) ligand 9 Ccr2 3,35195 0,000802 chemokine (C-C motif) receptor 2 Ccr5 3,23358 0,001222 chemokine (C-C motif) receptor 5 Cd14 6,13325 8,61E-10 CD14 antigen Cd36 2,94367 0,003243 CD36 antigen Cd44 4,89958 9,60E-07 CD44 antigen Cd81 6,49623 8,24E-11 CD81 antigen Cd9 3,06253 0,002195 CD9 antigen Cdkn1a 4,65279 3,27E-06 cyclin-dependent kinase inhibitor 1A (P21) Cebpb 6,6083 3,89E-11 CCAAT/enhancer binding protein (C/EBP),
    [Show full text]
  • Scavenger Receptor 1 As a Candidate Gene Response
    A Quantitative Trait Loci Analysis to Map Genes Involved in Lipopolysaccharide-Induced Inflammatory Response: Identification of Macrophage This information is current as Scavenger Receptor 1 as a Candidate Gene of September 27, 2021. William B. Fulton, Roger H. Reeves, Motohiro Takeya and Antonio De Maio J Immunol 2006; 176:3767-3773; ; doi: 10.4049/jimmunol.176.6.3767 Downloaded from http://www.jimmunol.org/content/176/6/3767 References This article cites 37 articles, 12 of which you can access for free at: http://www.jimmunol.org/ http://www.jimmunol.org/content/176/6/3767.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists by guest on September 27, 2021 • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2006 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology A Quantitative Trait Loci Analysis to Map Genes Involved in Lipopolysaccharide-Induced Inflammatory Response: Identification of Macrophage Scavenger Receptor 1 as a Candidate Gene1 William B. Fulton,* Roger H.
    [Show full text]
  • Gene-Expression Profiling of Different Arms of Lymphatic Vasculature Identifies Candidates for Manipulation of Cell Traffic
    Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic Imtiaz Iftakhar-E-Khudaa, Ruth Fair-Mäkeläa, Anu Kukkonen-Macchia, Kati Elimaa,b, Marika Karikoskia, Pia Rantakaria, Masayuki Miyasakaa,c, Marko Salmia,d, and Sirpa Jalkanena,d,1 aMediCity Research Laboratory, University of Turku, 20520 Turku, Finland; bDepartment of Medical Biochemistry and Genetics, University of Turku, 20520 Turku, Finland; cWorld Premier International Immunology Frontier Research Center, Osaka University, 565-0871 Osaka, Japan; and dDepartment of Medical Microbiology and Immunology, University of Turku, 20520 Turku, Finland Edited by Jason G. Cyster, University of California, San Francisco, CA, and approved July 26, 2016 (received for review February 11, 2016) Afferent lymphatic vessels bring antigens and diverse populations immune response is created elsewhere in the body and it also de- of leukocytes to draining lymph nodes, whereas efferent lymphatics termines what types of cancer cells can leave the draining LN. allow only lymphocytes and antigens to leave the nodes. Despite Even though this phenomenon is well recognized, the molecular the fundamental importance of afferent vs. efferent lymphatics in mechanisms behind it are completely unknown. Therefore, we immune response and cancer spread, the molecular characteristics of used a genome-wide microarray approach followed by expression these different arms of the lymphatic vasculature are largely un- analyses at the protein level to identify molecules preferentially known. The objective of this work was to explore molecular differ- expressed either on afferent [subcapsular sinus (SS)] or efferent ences behind the distinct functions of afferent and efferent lymphatic [lymphatic sinus (LS), i.e., cortical and subcortical sinuses] murine vessels, and find possible molecules mediating lymphocyte traffic.
    [Show full text]
  • A Novel Lncrna LNC 000052 Leads to the Dysfunction of Osteoporotic
    Li et al. Cell Death and Disease (2020) 11:795 https://doi.org/10.1038/s41419-020-03006-7 Cell Death & Disease ARTICLE Open Access A novel lncRNA LNC_000052 leads to the dysfunction of osteoporotic BMSCs via the miR-96- 5p–PIK3R1 axis Mingyang Li1, Rong Cong2,LiyuYang1, Lei Yang1,YiqiZhang1 and Qin Fu1 Abstract Bone marrow-derived mesenchymal stem cells (BMSCs) in postmenopausal osteoporosis models exhibit loss of viability and multipotency. Identification of the differentially expressed RNAs in osteoporotic BMSCs could reveal the mechanisms underlying BMSC dysfunction under physiological conditions, which might improve stem cell therapy and tissue regeneration. In this study, we performed high-throughput RNA sequencing and showed that the novel long non-coding RNA (lncRNA) LNC_000052 and its co-expressed mRNA PIK3R1 were upregulated in osteoporotic BMSCs. Knockdown of LNC_000052 could promote BMSC proliferation, migration, osteogenesis, and inhibit apoptosis via the PI3K/Akt signaling pathway. We found that both LNC_000052 and PIK3R1 shared a miRNA target, miR-96-5p, which was downregulated in osteoporotic BMSCs. Their binding sites were confirmed by dual-luciferase assays. Downregulation of miR-96-5p could restrain the effects of LNC_000052 knockdown while upregulation of miR-96-5p together with LNC_000052 knockdown could improve the therapeutic effects of BMSCs. In summary, the LNC_000052–miR-96-5p–PIK3R1 axis led to dysfunction of osteoporotic BMSCs and might be a novel therapeutic target for stem cell therapy and tissue regeneration. 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; Introduction are widely employed in the treatment of various diseases, Bone marrow-derived mesenchymal stem cells (BMSCs) such as acute pulmonary injury and myocardial injury5,6.
    [Show full text]
  • Investigating Reptin Function in Acute Myeloid Leukaemia
    INVESTIGATING REPTIN FUNCTION IN ACUTE MYELOID LEUKAEMIA Elena Armenteros Monterroso Developmental Biology and Cancer Section Institute of Child Health University College London A thesis submitted for the Degree of Doctor of Philosophy 2017 DECLARATION I, Elena Armenteros Monterroso, confirm that the work presented in this thesis is my own. Where information has been derived from other sources, I confirm that this has been indicated in the thesis. Signature …………………………………….. 2 ACKNOWLEDGEMENTS Firstly, I would like to express my sincere gratitude to my principal supervisor, Dr. Owen Williams, for his excellent advice, support and motivation during the past 4 years. I am extremely grateful for his guidance, but also for the freedom he has given me to pursue my own research. I could not have imagined having a better supervisor. I would also like to extend my gratitude to my second supervisor, Dr. Jasper de Boer. His help and advice have been invaluable. But also the fun environment he has provided in the lab, which made it easier to carry on during stressful times. I am also thankful to all the inspirational people working at the Cancer Section, particularly all the members of my lab, for their help and friendship during the past years. My sincere thanks also goes to all the members of the UCL Genomics team for their efficient work and their help with my sequencing experiments. I am also truly thankful to all my friends, both in the UK and in Spain, for providing the enthusiasm and support that I needed during my studies. I would like to specially thank Miriam, Clare and Heike for their friendship and fun times together.
    [Show full text]
  • Major Candidate Genes Associated with Risk of Hereditary and Sporadic Prostate Cancer
    Asian Oncology Research Journal 2(1): 1-13, 2019; Article no.AORJ.52920 Major Candidate Genes Associated with Risk of Hereditary and Sporadic Prostate Cancer Berjas Abumsimir1 and Moulay Mustapha Ennaji1* 1Team of Virology, Oncology, and Medical Biotechnologies, Laboratory of Virology, Microbiology, Quality, Biotechnologies/ETB, Faculty of Sciences and Techniques-Mohammedia, Hassan II University of Casablanca, Morocco. Authors’ contributions This work was carried out in collaboration between both authors. Authors BA and MME completed the review paper layout, writing and editing. Both authors read and approved the final manuscript. Article Information Editor(s): (1) Dr. Nina Tuncel, Department of Physics and Radiation Oncology, Akdeniz University, Turkey. Reviewers: (1) Heba Gamal Abd El-Aziz Nasr, Al-Azhar University, Egypt. (2) Jacilene Silva, Ceará State University, Brazil. (3) Swapna Kollabathina, Andhra University, India. (4) Deepak Sethi, Ravindra Nath Tagore Medical College, Udaipur, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/52920 Received 01 October 2019 Accepted 04 December 2019 Review Article Published 10 December 2019 ABSTRACT Prostate cancer risk factors gain more awareness in the world nowadays, due to the increasing incidents, which vary among different ethnic groups. Researches about genetic risk factors might help for more understanding of the initiation and development of prostate cancer and estimating risk values among individuals, and develop multi-perspective therapy approaches. Many efforts were achieved to study and evaluate high-risk variants associated with prostate tumors; through different populations. This evaluation depended on the high frequencies of these variants and the role of such variants in cell cycle and DNA repairing system.
    [Show full text]
  • Femstext Mae Huscup.Pdf
    The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2- Title mediated signaling pathway Mae, Masako; Iyori, Mitsuhiro; Yasuda, Motoaki; Shamsul, Haque; Kataoka, Hideo; Kiura, Kazuto; Hasebe, Akira; Author(s) Totsuka, Yasunori; Shibata, Ken-ichiro FEMS immunology and medical microbiology, 49, 398-409 Citation https://doi.org/10.1111/j.1574-695X.2007.00218.x Issue Date 2007-02-22 Doc URL http://hdl.handle.net/2115/43975 Type article (author version) File Information FEMStext mae huscup.pdf Instructions for use Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2-mediated signaling pathway Masako Mae1,2*, Mitsuhiro Iyori1*, Motoaki Yasuda1, Haque Mohammad Shamsul1, Hideo Kataoka1, Kazuto Kiura1, Akira Hasebe1, Yasunori Totsuka2 and Ken-ichiro Shibata1 Laboratories of Oral Molecular Microbiology1 and Oral & Maxillofacial Surgery2, Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Nishi 7, Kita 13, Kita-ku, Sapporo 060-8586, Japan. Running title: Enhancement of phagocytosis by a lipopeptide Keywords: Toll-like receptor 2, the lipopeptide FSL-1, phagocytosis, macrophage *: Masako Mae and Mitsuhiro Iyori are equally contributed to this work. Correspondence: Ken-ichiro Shibata, Laboratory of Oral Molecular Microbiology, Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Nishi 7, Kita 13, Kita-ku, Sapporo 060-8586, Japan. Phone: 81-11-706-4240. Fax: 81-11-706-4901. E-mail: [email protected] Abstract Enormous lines of evidence have been accumulated that Toll-like receptors (TLRs) function as sensors for microbial invasion.
    [Show full text]
  • PAI-1 Derived from Cancer-Associated Fibroblasts in Esophageal
    Laboratory Investigation (2021) 101:353–368 https://doi.org/10.1038/s41374-020-00512-2 ARTICLE PAI-1 derived from cancer-associated fibroblasts in esophageal squamous cell carcinoma promotes the invasion of cancer cells and the migration of macrophages 1,2 1 1,2 1 1,2 Hiroki Sakamoto ● Yu-ichiro Koma ● Nobuhide Higashino ● Takayuki Kodama ● Kohei Tanigawa ● 1,2 1,2 1 1 2 Masaki Shimizu ● Masataka Fujikawa ● Mari Nishio ● Manabu Shigeoka ● Yoshihiro Kakeji ● Hiroshi Yokozaki 1 Received: 18 May 2020 / Revised: 10 November 2020 / Accepted: 11 November 2020 / Published online: 12 December 2020 © The Author(s) 2020. This article is published with open access Abstract Cancer-associated fibroblasts (CAFs) contribute to the progression of various cancers. Previously, we reported the significance of CAFs in esophageal squamous cell carcinoma (ESCC); however, the functions of CAFs in the ESCC microenvironment remain unknown. To investigate CAFs’ function, we established an indirect coculture assay between human bone marrow-derived mesenchymal stem cells (MSCs) and ESCC cells. Cocultured MSCs expressed more fibroblast 1234567890();,: 1234567890();,: activation protein, one of the markers of CAFs, compared with monocultured MSCs. Therefore, we defined cocultured MSCs as CAF-like cells. To identify molecules associated with the ESCC progression in CAFs, we conducted a cDNA microarray analysis on monocultured MSCs and CAF-like cells to compare their gene expression profiles. We found that SERPINE1, which encodes plasminogen activator inhibitor-1 (PAI-1), was more abundant in CAF-like cells than in monocultured MSCs, and the PAI-1 derived from CAF-like cells induced the abilities of migration and invasion in both ESCC cells and macrophages by the Akt and Erk1/2 signaling pathways via the low-density lipoprotein receptor-related protein 1 (LRP1), which is a PAI-1 receptor.
    [Show full text]