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Margaret Mars Brisbin Okinawa Institute of Science and Technology (OIST) Graduate University Margaret Marine Biophysics Unit 1919-1 Tancha, Onna-son Mars Okinawa, 904-0495 Japan [email protected] [email protected] Brisbin BrisbinPlankton.com @MargaretBrisbin Education Okinawa Institute of Science and Technology (OIST) Graduate University, Okinawa, Japan, PhD candidate, 2014–present Dissertation: Characterization of Acantharea-Phaeocystis photosymbioses: distribution, abundance, specificity, maintenance and host-control Advisor: Satoshi Mitarai Stony Brook University, Stony Brook, New York, M.Sc. in Marine and Atmospheric Science, 2008–2010 Thesis: Characterization of antimicrobial activity present in the cuticle of American lobster, Homarus americanus Advisors: Anne E. McElroy & Bassem Allam St. John’s University, Queens, New York, B.Sc. in Biology, 2003–2007, graduated summa cum laude Fellowships & Japan Society for the Promotion of Science (JSPS) DC1 Graduate Research Grants Student Fellowship, three years of monthly stipend support, 2017–2020 JSPS fellowships are “awarded to excellent young researchers, as an opportunity to focus on freely chosen research topics and innovative ideas.” (21.9% success rate) JSPS Grant-in-Aid for Fellows, 2017–2020 Total award: $25,000 (out of a maximum of $30,000) NOAA/Sea Grant Graduate Student Fellowship, 2009–2010 Awards Student Excellence in Community Service Award, OIST, 2019 International Year of the Reef Award for Science Communication and Outreach, University of Queensland Coral Watch, April, 2018 Publications 6. Ares, A.*, Mars Brisbin, M.*, Sato, K. N., Martin, J. P., Iinuma, Y., Mitarai, S. (2019). Extreme storm-induced run-off causes rapid, context- dependent shifts in nearshore subtropical bacterial communities. bioRxiv. https://www.biorxiv.org/content/10.1101/801886v1. (submitted) * contributed equally 5. Mars Brisbin, M., Conover, A. E., Mitarai, S. (2019). Hydrothermal activity and water mass composition shape microbial eukaryote diversity and biogeography in the Okinawa Trough. bioRxiv. https://doi.org/ 10.1101/714816. (submitted) 4. Arora, J., Mars Brisbin, M., Mikheyev, A. S. (2019). The microbiome wants what it wants: microbial evolution overtakes experimental host- mediated indirect selection. bioRxiv. https://doi.org/10.1101/706960. (submitted) 1 3. Mars Brisbin, M. & Mitarai, S. (2019). Differential gene expression supports a resource-intensive, defensive role for colony production in the bloom-forming haptophyte, Phaeocystis globosa. Journal of Eukaryotic Microbiology. https://doi.org/10.1111/jeu.12727. 2. Mars Brisbin, M., Mesrop, L. M., Grossmann, M. M., Mitarai, S. (2018). Intra-host symbiont diversity and extended symbiont maintenance in photosymbiotic Acantharea (clade F). Frontiers in Microbiology, 9. https:// doi.org/10.3389/fmicb.2018.01998. 1. Mars Brisbin, M., McElroy, A. E., Pales Espinosa, E., Allam, B. (2015). Antimicrobial activity in the cuticle of the American lobster, Homarus americanus. Fish & Shellfish Immunology, 44(2), 542-546. https://doi.org/ 10.1016/j.fsi.2015.03.009. Conference Mars Brisbin, M. & Mitarai, S. (2019, March). Complementing high- Presentations throughput sequencing with high-throughput imaging to illuminate abundance and life history of photosymbiotic acantharians. Poster #50 presented at the ASLO 2019 meeting, San Juan, Puerto Rico. https:// doi.org/10.6084/m9.figshare.7751060.v2. Student Travel Award Recipient. Mars Brisbin, M. & Mitarai, S. (2018, Aug). Differential gene expression associated with colony formation in the bloom-forming haptophyte, Phaeocystis globosa. Oral Presentation at the PSA & ISOP Joint Meeting 2018, Vancouver, BC, Canada. https://doi.org/10.6084/m9.figshare. 6916274.v1. Mars Brisbin, M., Grossmann, M.M., Mitarai, S. (2018, Feb). Friend or Foe: A multiple meta ‘omics investigation into the nature of Acantharea- Phaeocystis photosymbiosis. Oral Presentation at the Ocean Sciences Meeting 2018, Portland, OR, U.S.A. Mars Brisbin, M., Emborski. C. K., Mikheyev, A. S. (2015, June). The role of gut bacteria in the inheritance of acquired metabolic phenotypes. Poster #57 presented at the Gordon Research Conference on Animal-Microbe Symbioses, Waterville Valley, NH, U.S.A. Open-source EukGeoBlast: Interactive geographic plotting of protist 18S BLAST hits. Community https://maggimars.github.io/eukGeoBlast/eGB.html. Resources Single-cell RNA extraction and cDNA library preparation from challenging marine protists (e.g. Acantharea). dx.doi.org/10.17504/ protocols.io.mvhc636. International The Carpentries Instructor Training, remote, Nov. 2019 Courses Imaging Flow Cytobot Users Meeting 2018, Woods Hole, MA, Nov. 2018 & Workshops Eco-DAS (Ecological Dissertations in the Aquatic Sciences), UH Manoa, HI, Oct. 2018 Data Intensive Biology Summer Institute (DIBSI) Next-Gen Sequence Analysis Workshop, UC Davis, CA, July 2018 2 Strategies and Techniques for Analyzing Microbial Population Structures (STAMPS), Marine Biological Laboratory, MA, August 2017 EMBL Course: RNA Sequencing Library Preparation, European Molecular Biology Laboratory, Heidelberg, Germany, March 2017 ICBM Summer School: Water column processes from coast to ocean, Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Germany, July 2015 Teaching Teaching Assistant, Data Intensive Biology Summer Institute (DIBSI) Next- Experience Gen Sequence Analysis Workshop, UC Davis, July 1–July 13, 2019 Taught RNA-seq lesson, assisted learners in other formal lessons and in bring-your-own-data breakout sessions R programming language Skill Clinic tutor, OIST, May–August 2018 Assisted learners in weekly 3-hr meet-ups consisting of online activities and projects. Additionally assisted learners with personal data analyses. Academic Instructor and Trip Leader, Broadreach, Raleigh, NC, 2011– 2014 Summer semester study abroad courses in Marine Biology, Marine Conservation and Policy, Ichthyology, and Shark Behavior and Conservation accredited by Lesley University, MA, U.S.A. Adjunct Instructor, Georgia Military College, Valdosta, GA, 2013–2014 5-credit introductory Marine Biology blended online/classroom course Middle School Science Teacher, Salam Academy, Albuquerque, NM, 2011–2013 6th, 7th, and 8th grade science, advisor to Science and Math Clubs, school-wide science fair coordinator Teaching Assistant, Stony Brook University, Stony Brook, NY, 2008-2010 • Environmental Microbiology, lab and lecture (1 semester) • General Chemistry II, two lab sections and a help section (1 semester) • Women In Science & Engineering (WISE) Introduction to Research in Marine Science (1 semester) Service Nerd Nite Okinawa, Organizer and chapter founder. Okinawa, 2018– present Nerd Nite is a monthly lecture series aimed at enhancing community interaction with science and technology. Awards $500 micro-grants to promote science communication. Ecology & Evolution Preprint Journal Club, Organizer and leader, OIST, 2018–2019 As eLife Ambassador, I established the club, facilitated meetings, and published preprint reviews with PREreview. Environmentally Conscious OISTers (ECO) club, Founder and student- leader, OIST, 2017–present ECO advocates for including sustainability in university policy and organizes community events. Awarded $2000 in grant funding from The Lonely Whale Foundation. 3 OIST Internal Seminar Series, Coordinator, OIST, 2016–present Bi-weekly general audience research seminars featuring OIST PhD students and postdocs, including practice talks and feedback sessions. Academic Officer (Elected Position) to the Student Council, OIST, 2017 Represented students on educational issues to university leadership and opened new avenues for student feedback. Established the Students’ Choice Teaching Award to recognize excellent teaching and mentoring. Media “Marine ecology and ocean activism”, episode 206, Wonderlabs Podcast, Chris Richardson http://wonderlabs.libsyn.com/206-marine-ecology-and- ocean-activism “Symbiotic Plankton: Providers or Parasites?”, by Sophia Protheroe, OIST news center, May 21, 2018 https://www.oist.jp/news-center/news/2018/5/21/symbiotic-plankton- providers-or-parasites “Visitors to Okinawa invited to be citizen scientists as tourism takes its toll on coral”, by Jessica Kozuka, The Japan Times, April 15, 2018 https:// www.japantimes.co.jp/community/2018/04/15/general/visitors-okinawa- invited-citizen-scientists-tourism-takes-toll-coral/#.XU0sLJMza34 Society Association for the Sciences of Limnology and Oceanography (ASLO) Memberships International Society of Protistologists (ISOP) American Society for Microbiology (ASM) Society for Women in Marine Science (SWMS) 4.
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  • Phaeocystis Cf. Globosa F
    HELGOL,~NDER MEERESUNTERSUCHUNGEN Helgolfinder Meeresunters. 49, 283-293 (1995) Trophic interactions between zooplankton and Phaeocystis cf. globosa F. C. Hansen Netherlands Institute for Sea Research; PO Box 59, 1790 AB Den Burg, The Netherlands ABSTRACT: Mesozooplankton grazing on Phaeocystis cf. globosa was investigated by laboratory and field studies. Tests on 18 different species by means of laboratory incubation experiments, carried out at the Biologische Anstalt Helgotand, revealed that Phaeocystis was ingested by 5 meroplanktonic and 6 holoplanktonic species; filtering and ingestion rates of the latter were determined. Among copepods, the highest feeding rates were found for Calanus helgolandicus and Temora longicornis. Copepods fed on all size-classes of Phaeocystis offered (generally 4-500 [~m equivalent spherical diameter [ESD]), but they preferred the colonies. Female C. helgolandicus and female 7". longicornis preferably fed on larger colonies (ESD > 200 [~m and ESD > 100 gm, respec- tively. However. a field study, carried out in the Marsdiep ~Dutch Wadden Seal showed phytoplank. ton grazing by the dominant copepod -femora longicorms to be negligible during the Phaeocysti, spring bloom. T longicornis gut fluorescence was inversely related to Phaeocystis dominance. Th~ hypothesis has been put forward that 7". 1ongicornis preferentially feeds on microzooplankton and b) this may enhance rather than depress Phaeocystis blooms. Results from laboratory incubatioc experiments, including three trophic levels - Phaeocystis cf globosa (algael. Strombidinopsis sp Iciliatel and Temora longicornis (copepod) - support this hypothesis. INTRODUCTION The colony-forming prymnesiophyte Phaeocystis cf. 91obosa builds up high bio- masses in the continental coastal areas of the North Sea during intense phytoplankton blooms tn spring and summer, where it can be the dominant species (Joiris et al., 1982; Veldhuis et al., 1986).
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    Deep-Sea Research II ] (]]]]) ]]]–]]] Contents lists available at SciVerse ScienceDirect Deep-Sea Research II journal homepage: www.elsevier.com/locate/dsr2 Giantism and its role in the harmful algal bloom species Phaeocystis globosa Walker O. Smith Jr.a,n, Xiao Liu a,1, Kam W. Tang a, Liza M. DeLizo a, Nhu Hai Doan b, Ngoc Lam Nguyen b, Xiaodong Wang c a Virginia Institute of Marine Science, College of William & Mary, Gloucester Pt., VA 23062, United States b Institute of Oceanography, Vietnam Academy of Science & Technology, 01 Cau Da, Nha Trang, Viet Nam c Research Center for Harmful Algae and Aquatic Environment, Jinan University, Guangzhou, China article info abstract The cosmopolitan alga Phaeocystis globosa forms large blooms in shallow coastal waters off the Viet Keywords: Nam coast, which impacts the local aquaculture and fishing industries substantially. The unusual Phaeocystis feature of this alga is that it forms giant colonies that can reach up to 3 cm in diameter. We conducted Colonies experiments designed to elucidate the ecophysiological characteristics that presumably favor the Size development of giant colonies. Satellite images of chlorophyll fluorescence showed that the coastal Envelope bloom was initiated in summer and temporally coincident with the onset of monsoonally driven DOC upwelling. While determining the spatial distribution of Phaeocystis was not feasible, we sampled it in Sinking the near-shore region. A positive relationship was found between colony size and colonial cell densities, in contrast to results from the North Sea. Mean chlorophyll a concentration per cell was 0.45 pg cellÀ1, lower than in laboratory or temperate systems.
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  • Differences in the Formation Mechanism of Giant Colonies in Two Phaeocystis Globosa Strains
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    bioRxiv preprint doi: https://doi.org/10.1101/461756; this version posted November 5, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 Differential gene expression supports a resource-intensive, defensive role for colony 2 production in the bloom-forming haptophyte, Phaeocystis globosa 3 4 Margaret Mars Brisbina and Satoshi Mitaraia 5 6 a Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, 7 Okinawa, Japan 8 9 Correspondence 10 M. Mars Brisbin, Marine Biophysics Unit, Okinawa Institute of Science and Technology 11 Graduate University, 1919-1 Tancha, Onna-son, Okinawa, Japan 12 e-Mail: [email protected] 13 14 Abstract 15 16 Phaeocystis globosa forms dense, monospecific blooms in teMperate, northern waters. Blooms 17 are usually dominated by the colonial morphotype—non-flagellated cells eMbedded in a secreted 18 Mucilaginous mass. Colonial Phaeocystis blooms significantly affect food-web structure and 19 function and negatively iMpact fisheries and aquaculture, but factors initiating colony production 20 reMain enigmatic. Destructive P. globosa blooms have been reported in tropical and subtropical 21 regions more recently and warm-water blooms could become more comMon with continued 22 cliMate change and coastal eutrophication. We therefore assessed genetic pathways associated 23 with colony production by investigating differential gene expression between colonial and 24 solitary cells in a warm-water Phaeocystis globosa strain. Our results illustrate a transcriptional 25 shift in colonial cells with Most of the differentially expressed genes downregulated, supporting a 26 reallocation of resources associated with colony production.
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  • Articles Combined (See, 5 %–32 % and 17 %–63 % Between 30–90 and 60–90◦ S, Re- E.G., Turner, 2015)
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  • Manganese and Iron Deficiency in Southern Ocean Phaeocystis Antarctica Populations Revealed Through Taxon-Specific Protein Indic
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  • COCCOLITHOPHORES Optical Properties, Ecology, And
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