Clear Whey Protein Isolate

INGREDIENTS strength and muscle recovery and reducing exercise- induced fatigue* • Provides optimal protein intake for the ageing population* • 100% Hydrolyzed Whey Protein*

Cherry, 19.5 OZ (1.2 LB) 546g Supplemental Facts

Doctor’s Best Clear Whey Protein Isolate is a water-soluble whey protein isolate powder that delivers the highest quality whey protein in a clear solution. It is produced by utilizing a proprietary filtration process that isolates native whey proteins in a highly concentrated form to provide maximum functionality and exceptional nutritive value, along with all the essential amino acids (EAAs) including the 3 BCAAs (branched-chain amino acids: Leucine, Isoleucine, and Valine) crucial for muscle growth.

While it is well-known that taking whey protein with BCAAs before any workout can help physical performances, Doctor’s Best Clear Whey Protein Isolate is the ideal whey protein isolate loaded with the complete spectrum of all three BCAAs that can be taken during your workout thanks to the exceptional clean taste it gives. By consuming BCAAs during training, it will help reducing the muscle breakdown, promoting endurance and refueling the lost energy and nutrition during the workout. As a result of its patented filtration process, Doctor’s Best Clear Whey Protein Isolate offers the utmost clean formula that is easily mixed and absorbed by the body and can be taken by competitive and recreational athletes. While ingestion of proteins is important for maintenance of a variety of body functions, whey protein has become a significant asset in medical field to improve bone, cardiovascular, and immunity health for the general population, from the young to the eldest.*1-5 In particular, in the search of maintaining a healthy active lifestyle, whey protein supplementation for older individuals has become an essential health nutritive value to fight against loss of muscle mass.*6-7 BENEFITS • Provides a very pure form of whey protein isolate with more than 90% protein with minimal lactose (<1%) and virtually no fat* • Provides a complete spectrum of all nine essential amino acids including the 3 BCAAs (Branched Chain Amino Acids) necessary for increasing muscle growth and lean body mass and decreasing muscle breakdown and soreness* • Has been specially formulated to be taken during workout for optimal results (it can also be taken before and after)* * These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. • Helps improve overall performance by increasing EXTENDED BENEFITS (Branched Chain Provides a very pure form of whey protein Amino Acids) isolate with more than 90% protein with minimal necessary for lactose (<1%) and virtually no fat. increasing muscle Whey protein is a type of milk protein produced as a byproduct growth and lean of cheese production and the term “whey” refers to the body mass and decreasing muscle breakdown complex mixture that is separated from the liquid remaining and soreness.* after milk has been curdled and strained. Although, whey protein is well-known worldwide as a superfood, building muscle and improving exercise endurance, it has been used in Helps improve overall performance by various products such as in infant formulas, food supplements, increasing strength and muscle recovery and beverages, and in different applications of medical field to meet 7-8 reducing exercise-induced fatigue.* a variety of health goals for people of all ages.* Historically, Numerous studies in animals and humans have shown that the intake of whey in the form of liquid can be dated back to whey proteins can have tremendous nutraceutical potential around 2500 years ago when Hippocrates recommended for athletes.*7,14 They contain amino acids to support body some drinks to enhance the immune system, power, and the muscle growth rate of the body.*9 Peach Mango, 18.9 OZ (1.17 LB) 529.2g However, based on current research, not all whey proteins are Supplemental Facts equal. Indeed, whey is available in three major forms: Whey Protein Concentrates, Whey Protein Isolates, and Whey Protein Hydrolysates. When it comes to building muscle growth and improving overall physical endurance, whey protein isolates are the form of supplementation preferred. Whey isolate undergoes different processing than whey concentrate, which results in the isolate containing more protein with less carbs and fat per serving.2 Moreover, whey protein isolates are both easily digested and have excellent metabolic efficiency. They contain the highest percentage of protein source. Doctor’s Best Clear Whey Protein Isolate uses a unique process of filtration allowing to produce a whey isolate powder containing 90% or more protein by weight. In addition to having lower total carbohydrate content, whey isolate also has lower lactose content. This means also that it may be a better choice for those who are lactose intolerant. In addition, Doctor’s Best Clear Whey Protein Isolate contains all the essential amino acids including the three branched-chain amino acids known to stimulate protein synthesis and muscle growth, which is important in muscle building and muscle retention.*10

Has been specially formulated to be taken during workout for optimal results (it can also be taken before and after). It is well-known that whey protein supplements are beneficial to athletes and bodybuilders in promoting muscle growth and improving overall physical endurance and are overall the best protein sources to take when compared to other types of protein supplements (casein and soy).*11 Traditionally, dietary protein supplements are ingested before or after physical exercise. But based on recent new studies, the potential to modulate protein synthesis by taking whey protein supplementation during exercise (or intra-workout) may also be beneficial to the body. Doing so, this could increase muscle protein synthesis rates, stimulate net muscle protein augmentation, and minimize protein degradation, thereby lead to greater gains in fat-free mass and overall enhance physical endurance.*12-13 Doctor’s Best with Clear Whey IsolateTM has a unique formulation that provides a product clear in solution, clean in taste, and without the texture of common whey protein allowing athletes and any individuals looking to increase their protein intake to ingest whey protein isolates during workout for optimal results.*

Provides a complete spectrum of all nine * These statements have not been evaluated by the Food and Drug Administration. essential amino acids including the 3 BCAAs This product is not intended to diagnose, treat, cure or prevent any disease. composition and all the essential amino acids that the body exercise-induced strength loss cannot synthetize to enhance athletic performance.* In caused by muscular fatigue particular, with its unique proprietary filtration process, Clear from working out.*38-40 Whey Protein Isolate is able to isolate native whey proteins in a highly concentrated form to provide maximum functionality, delivering consequently the purest proteins including all the CLINICAL STUDIES EAAs and the three BCAAs (leucine, isoleucine, and valine) vital A study investigated the ability of whey-protein and leucine for muscle growth, strength and recovery, and fat loss.*15-18 supplementation in enhancing physical, body composition, and cognitive performance. Thirty participants completed a Other studies have also linked a positive relationship between modified Air Force fitness test, a computer-based cognition BCAAs intake and the reduction of muscle damage associated test, and a dual-energy X-ray-absorptiometry scan for body during endurance exercise.*19 With respect to lean body mass, composition before and after supplementing their daily diet for Cermak et al. performed a meta-analysis that examined the 8 weeks with either 19.7 g of whey protein and 6.2 g leucine (WPL) impact of protein supplementation on changes in strength or a calorie-equivalent placebo (P). Bench-press performance and fat-free mass. Data from 22 separate published studies increased significantly from Week 1 to Week 8 in the WPL group, that included 680 research participants were included in the whereas the increase in the P group was not significant. Push- analysis. These authors concluded that protein supplementation up performance increased significantly for WPL group, and P demonstrated a positive effect of fat-free mass and lower-body strength in both younger and older participants.*20 Similarly, Fruit Punch, 18.9 OZ (1.17 LB) 529.2g in 2018, Morton et al. performed a meta-review and meta- regression involving 49 studies and 1863 participants and found Supplemental Facts that protein supplementation can contribution to increases in fat-free mass.*21

Recent studies and scientific reviews have highlighted that BCAAs, particularly Leucine, has critical biochemical actions involved in stimulating protein synthesis, inhibiting protein degradation, augmenting the activity of protein and availability of specific eukaryotic cell initiation factors.22-26 In particular, leucine is the main BCAA that modulates the function of proteins in gene translation via activating of rapamycin complex-1 (mTORC1), the mTOR signaling pathway known to be involved in cellular growth, muscle protein synthesis and muscle repair.27-31 While BCAAs are key to increase stimulation of mTORC1 activity, the ingestion of BCAAs alone, without the concurrent ingestion of other EAAs, intact protein or other macronutrients, does not maximally stimulate muscle protein synthesis following exercise despite the stimulation of mTORC1 translation initiation pathways.*18 Doctor’s Best Clear Whey Protein Isolate will deliver all of these essential nutraceutical organic compounds for the best health and physical outcomes.*

Provides optimal protein intake for the ageing population* Maintaining an independent healthy lifestyle is one of the top priorities for the elderly population. Unfortunately, one of the major drawbacks of growing older is the loss of skeletal muscle mass, strength and function, and the gain of body fat that progressively occur due to body composition shifts. Research suggests that one of the potential mechanisms contributing to the loss of skeletal muscle mass is reduced muscle protein synthesis, which was shown to decline by 3.5% per decade.32 Scientific data suggest that increasing dietary protein intake may counterbalance muscle loss in older individuals due to the increased availability of amino acids, which stimulate muscle protein synthesis.*33-36

In addition, based on recent metabolic and epidemiological studies, there is sufficient evidence that protein intake higher than the current dietary recommendations (0.8 g/kg/day) is beneficial for most older adults. Higher protein intakes are associated with increased muscle protein synthesis, which is correlated with increased muscle mass and function. This, in turn, is linked to improved physical function.*37 Other studies have been conducted in the older population to show the * These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. positive effect of taking whey protein supplements on reducing group showed a nonsignificant increase. Total mass, fat-free A study investigated the mass, and lean body mass all increased significantly in the WPL response of myofibrillar- group but showed no change in the P group. No differences muscle protein synthesis to were observed within or between groups for crunches, chin- ingestion BCAAs only (i.e., ups, 3-mile-run time, or cognition. The authors conclude that without concurrent ingestion supplementing with whey protein and leucine may provide of other EAA, intact protein, an advantage to people whose performance benefits from or other macronutrients) following resistance exercise in ten increased upper body strength and/or lean body mass.*15 young participants. These young athletes completed two trials, ingesting either 5.6 g BCAAs or a placebo drink immediately A pilot double-blind cross-over designed study aimed to test after resistance exercise. Myofibrillar-muscle protein synthesis whether BCAA supplementation enhances exercise capacity was measured during exercise recovery with a primed, and lipid oxidation in glycogen-depleted subjects. Volunteers constant infusion of a marker and muscle biopsies pre and were randomly assigned to either the BCAAs (300 mg/kg/ 4 h-post drink ingestion were collected. Blood samples were day) or the placebo (maltodextrin) for 3 days. On the second collected at time-points before and after drink ingestion. Based day, subjects were submitted to an exercise-induced glycogen on the data obtained, myofibrillar-muscle protein synthesis was depletion protocol. They then performed an exhaustive exercise 22% higher in BCAAs group than placebo group. They authors test on the third day, after which time to exhaustion, respiratory exchange ratio (RER), plasma glucose, and other parameters were determined. Results showed BCAAs supplementation Green Apple, 18.8 OZ (1.16 LB) 525g promoted a greater resistance to fatigue when compared Supplemental Facts to the placebo (+17.2%). Moreover, subjects supplemented with BCAAs showed reduced RER and higher plasma glucose levels during the exhaustive exercise test. In conclusion, the authors suggested that BCAAs supplementation may increase resistance to fatigue and enhances lipid oxidation during exercise in glycogen-depleted subjects.*16

A study investigated the effects of daily oral L-leucine ingestion on strength, bone mineral-free lean tissue mass (LTM) and fat mass (FM) of living humans during a 12-week resistance-training program. Twenty-six initially untrained men were randomly assigned to ingest either 4 g/day of L-leucine or a corresponding amount of lactose (placebo group). All participants trained under supervision twice per week following a prescribed resistance training program using eight standard exercise machines. Testing took place at baseline and at the end of the supplementation period. Results showed the leucine group demonstrated significantly higher gains in total 5-repetition max strength (sum of 5-RM in eight exercises). The percentage total 5-RM strength gains were 40.8% and 31.0% for the leucine and placebo groups respectively. The authors concluded that 4 g/ day of L-leucine supplementation may be used as a nutritional supplement to enhance strength performance during a 12- week resistance training program of initially untrained male participants.*24

A study examined the effects of BCAAs supplementation on markers of muscle damage induced via a sport specific bout of damaging exercise (100 consecutive drop-jumps) in twelve trained volunteers. They were randomly assigned to a supplement or placebo group. Creatine kinase (CK), maximal voluntary contraction (MVC), muscle soreness (DOMS), and other markers of muscle damage were measured. All variables were measured immediately before the damaging exercise and at 24, 48, 72 and 96 h post-exercise. Results showed significant time effect for all variables. There were significant group effects showing a reduction in CK efflux and muscle soreness in the BCAA group compared to the placebo. Furthermore, the recovery of MVC was greater in the BCAA group. The authors concluded that BCAAs administered before and following damaging resistance exercise reduces indices of muscle damage and accelerates recovery in resistance-trained males. They concluded that BCAAs provided greater bioavailability of substrate to improve protein synthesis and thereby the extent of secondary muscle damage associated with strenuous * These statements have not been evaluated by the Food and Drug Administration. resistance exercise.*41 This product is not intended to diagnose, treat, cure or prevent any disease. concluded that ingesting BCAAs alone can increase post- exercise stimulation of myofibrillar-muscle protein synthesis through the mTORC1 signaling.18

A study investigated the effect of protein co-ingestion with carbohydrate on muscle protein synthesis during resistance- type exercise. Ten healthy males were studied in the evening after they consumed a standardized diet throughout the day. Subjects participated in two experiments in which they ingested either carbohydrate (CHO) or carbohydrate with protein (CHO- PRO) during a 2-h resistance exercise session. Subjects received a bolus of test drink before and every 15 min during exercise. Blood and muscle biopsies were collected to assess whole body and muscle protein synthesis rates during exercise. Protein coingestion lowered whole body protein breakdown rates by 8.4 3.6% compared with the ingestion of carbohydrate only, and augmented protein oxidation and synthesis rates by 77 17 and 33 3%, respectively. Consequently, whole body net protein balance was negative in CHO group, whereas a positive net balance was achieved after the CHO-PRO treatment. The authors concluded that even protein co-ingestion stimulates whole body and muscle protein synthesis rates during resistance-type exercise.*12

A clinical evaluated the effect of a 12-week leucine-rich amino acid supplementation in combination with moderate training among forty-eight healthy subjects (aged 55-75 years old) who exercised for 30 minutes three times per week and received either a leucine-rich amino acid supplementation or a placebo. Before and after supplementation, volunteers performed an exhaustive eccentric exercise protocol. Many factors and markers (i.e. Maximal concentric strength, muscle soreness, creatine kinase),were assessed before exercise and after 3, 24, 48 and 72 hours. Results showed the supplementing with leucine resulted in reduced loss of strength at 0 and 3 hours after downhill walking compared with the placebo. The principle findings show that leucine-rich amino acid supplementation can counteract the negative effects of eccentric exercise. The treatment resulted in a reduction of exercise-induced strength loss.*39

The VIVE-2 Study, a Randomized, Double-Blind, Placebo- Controlled Trial, was conducted to examine the impact of Nutritional Supplementation with Physical Activity on Muscle Composition in Mobility-Limited among one hundred and forty- nine Older Adults (age: 78.5 (5.4) years). All subjects participated in a physical activity program. Subjects were randomized to consume a daily nutritional supplement (150 kcal, 20 g whey protein, 800 IU vitamin D, 119 mL beverage) or placebo. In a pre-specified secondary analysis, total- body composition (dual energy X-ray absorptiometry), thigh composition (computed tomography), and muscle strength, power, and quality were assessed before and after the 6-month intervention. After the intervention period, the treatment and placebo groups demonstrated improvements in muscle strength, body composition, and thigh composition. Nutritional supplementation lead to further losses of intermuscular fat and increased normal muscle density compared to the placebo. The authors concluded that nutritional supplementation provided additional benefits to mobility-limited older adults undergoing exercise training.*40

* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. 24. Ispoglou T et al. Daily SCIENTIFIC REFERENCES L-leucine supplementation 1. Solak BB & Akin N. Health benefits of whey protein: A Review. J in novice trainees during Food Sc Engineering. 2012;2:129-137. a 12-week weight training 2. Shankar JR & Bansal GK. A study on health benefits of whey program. Int J Sports Physiol proteins. Inter J Adv Biotechnol Res. 2013;4:15-19. Perform. 2011;6:38–50. 3. Patel S. Emerging trends in nutraceutical applications of whey 25. Fouré A & Bendahan D. protein and its derivatives. J Food Sci Technol. 2015;52:6847–6858. Is branched-chain amino acids supplementation an efficient 4. Witard OC et al. Protein Considerations for optimizing skeletal nutritional strategy to alleviate skeletal muscle damage? A muscle mass in healthy young and older adults. Nutrients. 2016, 8, systematic review. 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Leucine supplementation of a low- protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: A double-blind, randomized trial. Am. J. Clin. Nutr. 2014, 99, 276–286. 23. Anthony J.C et al. Orally administered leucine stimulates protein synthesis in skeletal muscle of postabsorptive rats in association * These statements have not been evaluated by the Food and Drug Administration. with increased eIF4F formation. J. Nutr. 2010, 130, 139–145. This product is not intended to diagnose, treat, cure or prevent any disease.

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