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Asian Pac J Trop Biomed 2014; 4(3): 209-212 209

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Asian Pacific Journal of Tropical Biomedicine

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Document heading doi:10.1016/S2221-1691(14)60233-1 2014 by Asian Pacific Journal of Tropical Biomedicine. All rights reserved. 襃 Survey on cattle ticks in Nur, north of

Ashkan Ghasemi Moghaddam1, Mozafar Razavi Seyed1, Mehrshad Rasouli1, Sajad Hosseinzade1, Mohammad Mehdi * Darvishi2, Rakhshanpour3, Mohammad Taghi Rahimi4,5 1Young Researchers Club, Islamic Azad University, Branch, Iran 2Department of Veterinary Parasitology, Semnan University, Semnan, Iran 3Department of Medical Parasitology and Mycology, School of Public Health, University of Medical Sciences, Tehran, Iran 4Department of Parasitology and Mycology, School of Medicine, University of Medical Science, , Iran 5Young Researchers Club, Islamic Azad University, Babol Branch, Iran

PEER REVIEW ABSTRACT

Peer reviewer Objective: To survey the prevalence of cattle ticks in and prepare a list of tick fauna Mohammad Reza Youssefi, Assistant inMethods: this district. Professor, Department of Veterinary This investigation was carried out on 150 head of cattle ticks of rural areas of Nur Parasitology, Islamic Azad University, city which is located in during spring and summer seasons of 2011. The Bbabol-Branch. collectedResults: ticks were identified using light microscope and available systematic keys. +9811148631 1 563 Tel: A total numberIxodes of ricinus ticks were isolated from examinedBoophilus cattle and annulatus their genus and E-mail: [email protected] numbers including: 51% (111 male and 691 female) and 49% (83 ) Comments maleConclusions: and 678 female , respectively. Results of the current investigationIxodes indicate ricinus the presenceBoophilus of two species annulatus of acarine This is a good study in which the ectoparasites which have potential health risk and . More authors surveyed ticks of cattle in Nur studies are required to increase our data concerning ticks and other ectopreasites of ruminants County. in other areas of Mazandaran province and should be noted to their ability in transmission of The results are valuable as there is infectious agents. not published data regarding ticks of livestock animals in this area. In addition, the proba-ble dangers of the causative agent of disease which are KEYWORDS transmitted by the isolated ticks were highlighted properly. Acarine ecotoparasites, Tick, Livestock, Cattle, Public health risk, Iran Details on Page 211

1. Introduction irritation and anemia. Moreover, ticks are responsible for serious economic losses of animal owners particularly in Ticks ( Arachnida: Acari: Parasitiformes ) are livestock industries[1,2]. Ticks are classified into two major hematophagous arthropods and obligatory ectoparasites families, Ixodidae (hard ticks) and Argasidae (soft tick). The which are considered significant in both veterinary and family Ixodidae comprises nearly 700 species including two medical field by acting as vector of serious pathogens major morphological and phylogenetic groups which are the Babesia Thileria Rickettsia Anaplasma such as , , , spp. Metastriata and the Prostriata. They have ability of infesting Borrelia and . When these obligatory ectoparasites attach a wide variety of hosts such as mammals, birds, reptiles and to a host for the aime of blood sucking, they induce skin amphibians. In addition, hard ticks are distributed broadly

*Corresponding author: Mohammad Taghi Rahimi, Department of Parasitology and Article history: Mycology, School of Medicine, Mazandaran University of Medical Science, Sari, Iran Received 19 Dec 2013 and Young Researchers Club, Islamic Azad University, Babol Branch, Iran. Received in revised form 22 Dec, 2nd revised form 30 Dec, 3rd revised form 10 Jan 2013 Tel: +989122930625. Accepted 25 Feb 2014 E-mail: [email protected] Available online 28 Mar 2014 Foundation Project: Supported by the Young Researchers Club, Islamic Azad University, Babol Branch (Grant No. BPJ1391). Ghasemi Moghaddam Ashkan et al./Asian Pac J Trop Biomed 2014; 4(3): 209-212 210 throughout the world[3,4]. Thereafter, isolated ticks were counted and separately The prevalence of the tick species which are involved on stored in 70% ethanol, labeled with the date until the species the transmission and their geographical distribution for the determination. Afterwards, all of crucial characters of tick purpose of control and tick borne diseases are a matter of samples were studied precisely. Their identification was high importance. Although Nur County has considerable undertaken based on taxonomic and structural differences superficial water resources which prepare an ideal area of the species by means of stereoscope (Nikon SMZ1500) for and animal husbandry, there is not enough and light microscope (Nikon. Microphoy-FXA) which was information concerning tick fauna of different hosts in this equipped by camera (Memmert D-91126) according to district. Therefore, the current investigation was conducted available systematic keys[5-7]. to survey the prevalence of cattle ticks in Nur County and prepare a list of tick fauna in this district. 3. Results

2. Materials and methods During the study, among 11 villages, a total number of 1 563 ticks were isolated from 150 examined cattle from 25 herds. 2.1. Study area The mean intensity for each animal was 10.42. Two tick genera and species were observed and recognized during ° ′ ″ ° ’ ° ′ ″ 2 Ixodes ricinus I. ricinus Nur County (36 34 25 N, 52 40 52 00 50 E) (2 675.00 km ) is the study including: ( ) 802 (51%) (111 Boophilus annulatus B. annulatus located on the coast and belongs to Mazandaran male, 691 female) and ( ) 761 province. This area embraces abundant superficial water (49%) (51 male and 710 female) (Figures 2 and 3). Totally 1 401 resources which is proper for agriculture and animal female and 162 male ticks were extracted from examined husbandry. This district contains pastures and forests which cattle. Moreover, no soft tick (Argasidae family) was are favorable for cattle grazing (Figure 1). observed.

µ 1 000 m

Ardabil East Azarbaijan Caspian Sea

N W orth est Khorasan Azarbaijan Zanjan Gilan Golestan

Qazvin Mazandran Razavi Kurdestan Khorasan

Hamedan TEHRAN Semnan Kermanshah Markazi Qom

Lurestan llam Esfahan

Chahar Mahal South Khuzestan va Bakhtiari Yazd Khorasan

Kohkiuyeh va Boyer ahmad K erman Figure 2. I. ricinus Sistan va Extracted tick ( ) from cattle. Baluchestan Bushehr Hormozgan µ 1 000 m

Sea of Oman Figure 1. Map of Iran, highlighting the position of Nur County in Mazandaran province.

2.2. Tick collection

An investigation was carried out on ticks of 150 heads of cattle from 25 herds of 11 villages during the hot and humid months (the spring and summer) of 2011 from rural areas of Nur County, Mazandaran province, Iran. Tick sampling was undertaken on all body of each animal at different intervals. Figure 3. B. annulatus Extracted tick ( ) from cattle. Ghasemi Moghaddam Ashkan et al./Asian Pac J Trop Biomed 2014; 4(3): 209-212 211 4. Discussion Hy. marginatum Hy. anatolicum (12 5%) (5 2%) Hy. asiaticum . Hy., detritum excavatumDermacentor . , (0 6%) (0 2%) B.. annulatus, Rh. . bursa, and I (0 1%) n the present investigation, two tick generaI. ricinusand species spp. . and and were the most I (51%) [16] from B.xodidae annulatus family were found, including prevalent tick species . (49%) I T 15 and . n addition, the number Ixodesof female he following ixodid tick species were recognized over S Z ticks was noticeably higher than male. pecies of are the survey period fromBoophilus cattle, kohlsisheep and goatsR. sanguineus in agros L (3 6%) vectors of major Borreliadiseases burgdorferisuch as yme disease, caused by mountainousRh. bursa areas: Rhipicephalus . turanicus (4 5%) (21 9%) (2 9%) the spirochaete , human granulocytic Dermacentor. , niveus . , Dermacentor raskemensis . , R M (12 9%) (4 1%) ehrlichiosis, ocky ountain spotted fever tick paralysis, Dermacentor marginatus . , Haemaphysalis punctata . , P [8,9] (7 3%) owassan encephalitisB. annulatus virus and babesiosis . Hyalomma parva . , Hyalomma choldokovskyi I (3 5%) (0 6%) n addition, belongs to subfamily . , Hy. anatolicum excavatum . , Hy. anatolicum R T A (2 0%) (4 8%) hipicephalinae. hey are all one-host ticks. ll stages anatolicum. , Hy. asiaticum asiaticum. , Hy. (5 2%) (7 3%) including larval, nymphal, and adult spend on the sameBoophilus host marginatum marginatum. , Hy. detritum . detritum, S (13 0%) and this is a rare features among ticks. pecies of . , and Rhipicephalus C C (5 9%)[17] I S are vectors of rimean- ongo hemorrhagic fever, ganjon . Hyalomma. n an investigation in avajbolgh, N T K [18] I viruses, bhanja virus inAnaplasma igeria and marginale hogoto virus in enya. and were the most obtained ticks . Hyalomman another M K oreover, rickettsia Boophilus is carried to similar study in erman, prevailing species was A B [19] cattle by all three species of in frica. Boophilusy far, the in examined cattles . most important disease transmitted by a species of The authors draw a conclusion based on the results is Texas cattle fever, also called red-water fever. The agent of current investigation that there is a probable risk of B. bigemina[10] I. ricinus B. annulatus of this disease is a piroplasm, B. bigemina. disease which is transmitted by and . I U S 1906 T Boophilusn the nited tates in , transmitted herefore, continuous parasitological studies and control by and it caused annual losses of 100 million programs are greatly required in order to decrease the dollars[11]. In Tanzania, the annual cost of diseases which spread of tick infestation in these areas. As tick dispersal was estimated by ticks was computed at 364 million may occur all the time owing to tick hosts movement and dollars[12]. Estimations of total economic impact of tick climate change. The domestic ruminant infestation must parasitism is not easy precisely due to many contributing not be neglected and must be taken serious because of two factors, including reduction of weight gaining, decrease of and three host ticks. And this important fact demonstrates milk production, expense of tick control, nutritional state their prominent role in transmitting and spreading several I and breed of cattle. et al dangerous diseases both in humans and animals. n According to literature review, Shemshad . introduced addition, further researches are required due to considerable the following tick faunaHaemaphysalis from cattle, sheep concinna and goats in unexplored area of our country in order to increase our B Z C (0 63%) Haemaphysalisoeen ahra ounty:sulcata Hyalomma anatolicum . , knowledge about tick and ecotoparasites of livestock and (12 66%) I Hy. anatolicum . Hyalomma, asiaticum Hy. their infestations and probable zoonoses diseases in ran. ( ) (3 80%) ( asiaticum Hyalomma . , detritum Hy. detritum ) (3 16%) ( ) (5 70%) Hyalomma .dromedarii, Hyalomma marginatum . , Conflict of interest statement (28 48%) Hy. marginatum . Hyalomma, schulzei ( ) (13 29%) (1 89%) Rhipicephalus bursa Rh.. bursa, Rhipicephalus . , ( ) (3 16%) sanguineus R. sanguineus . and ’ We declare that we have no conflict of interest. ( ) (3 16%) T Hyalomma dromedarii. , and for akistanHy.s (9 86%) marginatumlivestock were Hyalomma schulzei . , R. Acknowledgements (13 29%) (1 89%) sanguineus . , . and (3 16%) [13] . , respectively . Ixodes, Boophilus, I K (C ) W M S Dermacentor, n elardasht Haemaphysalis, halous Hyalomaarea , Rhipicephalus e wish to express our sincere thanks to r. oleymani for andI. ricinus his kind collaboration and assistance and also his valuable were reported from cattle and sheep that (26.8%) consultation during survey. This work was supported by the Y R C I A U B wasB. annulatus the most dominant tick species which was followed by oung esearchers lub, slamic zad niversity, abol (20.5%) in which our results are in agreement Branch (Grant No. BPJ1391). to their finding[14]. The occurrence of ticks on cattle in G C M 20 0% R.haemshahr sanguineus ounty, azandaran was reported . and Comments were the most abundant species in the study [15] area . Background In another investigation during 2004-2005, the following E species were identified from cattleB. ofannulatus eastern and westernRh. ctoparasites play a major role in transmission of disease M (51 3%) A bursaareas in azandaranHaemaphysalis province: punctata I. ricinus . , to livestock. nnually they impose a considerable burden (16.8%), (6.3%), (6.8%), on livestock industries. Moreover, we cannot ignore their Ghasemi Moghaddam Ashkan et al./Asian Pac J Trop Biomed 2014; 4(3): 209-212 212 importance as carrier of zoonose disease to human. York: Mcm Graw Hill; 2006; p. 570. I think, the background of the study contains important [5] E strada-Pena A, Bouattour A, Camicas JL, Walker AR. Ticks aspects of ticks both in medical and veterinary fields. of domestic animals in Mediterranean Region: a guide to identification of species. Zaragoza: University of Zaragoza; 2004, Research frontiers p. 43-131. Survey on ectoparasite, particularly ticks of livestock [6] K eirans JE, LitwakI TR. Pictorial key to the adults of hard ticks, family Ixodidae (Ixodida: Ixodoidea), east of the Mississippi River. which are usually in close contact with human, is of critical J Med Entomol 26 importance to economic and human health. As control 1989; (5): 435-448. of ectoprasites population is not easy, it requires a great [7] W alker AR, Bouattour A, Camicas JL, Estrada- Pena A, Horak amount of budget, energy and time. IG, Latif A, et al. Ticks of domestic animals in Africa: a guide to identification of species. London: Bioscience Reports; 2003, p. 74- Related reports 221. I K (C ) [8] R amesh G, Borda JT, Dufour J, Kaushal D, Ramamoorthy R, n elardashtI. ricinus halous area , the most dominant tick (26 8%) Lackner AA, et al. Interaction of the Lyme disease dpirochete was reported . that is in agreement with the Borrelia burgdorferi with brain parenchyma elicits inflammatory results of this research, althoughet al variety ofHy. their dromedarii tick species S mediators from glial cells as well as glial and neuronal apoptosis. were different. hemshad . reported as Am J Pathol 173 a the most dominant tick (28.48%) in Boeen Zahra County. 2008; (5): 1415-1427. T [9] S haw SE, Day MJ, Birtles RJ, Breitschwerdt EB. Tick-borne his contrast is probably due to different geographical and Trends Parasitol 17 climate condition. infectious diseases of dogs. 2001; : 74-80. [10] S hkap V, Kocan K, Molad T, Mazuz M, Leibovich B, Krigel Y, et al. and breakthroughs Anaplasma marginale Experimental transmission of field and the A. centrale Hyalomma excavatum Rhipicephalus G vaccine strain by , enerally, preparing data from an area where there is not sanguineus Rhipicephalus Boophilus annulatus Vet B and ( ) ticks. any information is a good idea. esides, introduction of two Microbiol 134 ticks which are considered as a serious danger not only for 2009; (3-4): 254-260. human but also for livestock industry is valuable. [11] M organ NO. Potential impact of alien arthropod pests and vectors of animal diseases on the U.S. livestock industry. In: Pimentel D, Applications CRC Handbook of pest management in agriculture editor. . 2 ed. This is noteworthy to know the prevalence and mean Boca Raton: CRC Press Inc.; 1991, p. 99-105. [12] K ivaria FM. Estimated direct economic costs associated with intensity of tick in this area because we can estimate the Trop Anim Health Prod tick-borne diseases on cattle in Tanzania. economic impact of tick parasitism in livestock and also we 38 are able to design a control program in order to prevent of 2006; : 291-299. probable zoonose disease and economical losses. [13] S hemshad M, Shemshad K, Sedaghat MM, Shokri M, Barmaki A, Baniardalani M, et al. First survey of hard ticks (Acari: Ixodidae) Peer review on cattle, sheep and goats in Boeen Zahra and Takistan counties, Asian Pac J Trop Biomed 2 This is a good study in which the authors surveyed ticks Iran. 2012; (6): 489-492. N C T [14] Y oussefi MR, Keighobadi M, Asnaashari MY. Ixodid tick species of cattle in ur ounty. he results are valuable as there is J Entomol infesting sheep and cattle in part (Chaloos). not published data regarding ticks of livestock animals in 5 this area. In addition, the probable dangers of the causative 2008; (1): 56-58. agent of disease which are transmitted by the isolated ticks [15] N asibeh HV, Zakkyeh T, Hassan V, Reza Y, Morteza HV, Ail OM. Survey of tick species parasiting domestic ruminants in were highlighted properly. Asian Pac J Ghaemshahr county, Mazandaran province, Iran. Trop Med 3 2010; (10): 804-806. [16] R azmi GR, Glinsharifdini M, Sarvi S. Prevalence of Ixodid ticks References Korean J Parasitol on cattle in Mazandaran province, Iran. 2009; 4 (45): 307-310. Veterinary ectoparasites: biology, pathology [1] W all R, Shearer D. [17] N abian S, Rahbari S. Occurrence of soft and hard ticks on and control Iranian J . 2nd ed. Oxford: Blackwell Science; 2001, p. 1-2, 27- ruminants in Zagros Mountainous Areas of Iran. Arthropod-Borne Dis 2 31, 66, 76, 80-81, 149-150, 166-167, 172-177, 179-181. 2008; (1): 16-20. [2] S hemshad K, Rafinejad J, Kamali K, Piazak N, Sedaghat MM, [18] E smailnia K, Aharipour V. Survey of sheep and goat in Shemshad M, et al. Species diversity and geographic distribution Savajbulagh and Abic area. Proceedings of the 5th National of hard ticks (Acari: Ixodoidea: Ixodidae) infesting domestic Iranian Congress of Parasitology; 2005 November 15-17; Tehran, Parasitol Res 110 ruminants, in , Iran. 2011; (1): Iran. p. 178. 373-380. [19] R adfar MH. Evaluation of tick ininfestaion in cattle, sheep, goat Lecture notes on medical entomology [3] S ervice MW. . Oxford: Black and camel in South East of Iran. Proceedings of the 5th National Well Scientific Publication; 1986, p. 338. Iranian Congress of Parasitology; 2005 November 15-17; Tehran, Foundations of parasitology [4] L arry SR, Janovy J. . 7th ed. New Iran. p. 193.