TPSG1 Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. TPSG1 polyclonal antibody Catalog: BS61326 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: by affinity-chromatography using epitope-specific im- Tryptases comprise a family of trypsin-like serine prote- munogen and the purity is > 95% (by SDS-PAGE). ases, the peptidase family S1. Tryptases are enzymatically Applications: active only as heparin-stabilized tetramers, and they are WB: 1:500~1:1000 resistant to all known endogenous proteinase inhibitors. Storage&Stability: Several tryptase genes are clustered on chromosome Store at 4°C short term. Aliquot and store at -25°C long 16p13.3. There is uncertainty regarding the number of term. Avoid freeze-thaw cycles. genes in this cluster. Currently four functional genes - al- Specificity: pha I, beta I, beta II and gamma I - have been identified. TPSG1 polyclonal antibody detects endogenous levels of And beta I has an allelic variant named alpha II, beta II TPSG1 protein. has an allelic variant beta III, also gamma I has an allelic DATA: variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as me- diators in the pathogenesis of asthma and other allergic and inflammatory disorders. Western blot (WB) analysis of TPSG1 polyclonal antibody at 1:500 di- Product: lution Lane1:HEK293T whole cell lysate Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, Lane2:RAW264.7 whole cell lysate Lane3:PC12 whole cell lysate 50% glycerol, pH7.2 Note: Molecular Weight: For research use only, not for use in diagnostic procedure. ~ 34 kDa Swiss-Prot: Q9NRR2 Purification&Purity: The antibody was affinity-purified from rabbit antiserum Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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UC San Francisco Electronic Theses and Dissertations
UCSF UC San Francisco Electronic Theses and Dissertations Title Protease-activated receptor-2 (PAR2) in epithelial biology Permalink https://escholarship.org/uc/item/2b49z9sm Author Barker, Adrian Publication Date 2013 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California ii To my nephews for being the light of my life To my parents for showing me the way To Philip, your love knows no bounds iii ACKNOWLEDGEMENTS Wow, what a journey! First, I’d like to thank my mentor and advisor, Dr. Shaun Coughlin, for giving me the encouragement and wisdom that I needed to succeed in your lab. One thing I will take away from this experience is how powerful collaboration can be. Having encountered labs that have not been willing to collaborate, you are an inspiration and role-model in your willingness to share your resources and knowledge with the scientific community and the academic world is a better place because of it. To my thesis committee members, Dr. Charly Craik & Dr. Zena Werb. Thank you for the conversations and encouragement. You have given me motivation and kind words in pivotal moments in my career and they have helped me tremendously; more than you’ll ever know. To all the members of the Coughlin lab. We’ve been through so much together, and many of you have been around since the first day I stepped foot into the lab. Extra special thanks to: Dr. Hilary Clay, for help with the zebrafish work and for fighting for my project when it felt like no one else cared; Dr.
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