1 2 3 4 5 6 7 Ecological and functional capabilities of an uncultured 8 Kordia sp. 9 10 Royo-Llonch M1,4, Sánchez P1, González JM2, Pedrós-Alió C3, Acinas SG1* 11 12 _____________________________________________________________________ 13 14 1 Department oF Marine Biology and Oceanography, Institut de Ciències del Mar (ICM), 15 CSIC, Barcelona, Spain. 16 2 Department oF Microbiology, University oF La Laguna, La Laguna, Spain 17 3 Systems Biology Program, Centro Nacional de Biotecnología (CNB), CSIC, Madrid, Spain 18 4 Departament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma 19 de Barcelona, Bellaterra, Spain 20 21 *Correspondence: 22 Dr. Silvia G. Acinas 23
[email protected]; 24 25 Keywords: SAG , Kordia, co-assembly, photoheterotrophy, metagenomics 26 1 27 Abstract 28 Cultivable bacteria are only a Fraction oF the diversity in microbial communities. However, 29 the oFFicial procedures For classiFication and characterization of a novel prokaryotic 30 species still rely on isolates. Thanks to Single Cell Genomics, it is possible to retrieve 31 genomes From environmental samples by sequencing them individually, and to assign 32 speciFic genes to a speciFic taxon, regardless oF their ability to grow in culture. In this 33 study, we perFormed a complete description oF the uncultured Kordia sp. 34 TARA_039_SRF, a proposed novel species within the genus Kordia, using culture- 35 independent techniques. The type material was a high-quality draFt genome (94.97% 36 complete, 4.65% gene redundancy) co-assembled using 10 nearly identical Single 37 AmpliFied Genomes (SAGs) From surFace seawater in the North Indian Ocean from the 38 Tara Oceans expedition.