PDIA2 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science PDIA2 Rabbit pAb Catalog No.: A12789 Basic Information Background Catalog No. This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic A12789 reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically Observed MW active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention 58kDa sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and Calculated MW reductase activity. 57kDa/58kDa Category Primary antibody Applications WB, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 64714 Q13087 IF 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2). Synonyms PDIA2;PDA2;PDI;PDIP;PDIR Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PDIA2 antibody (A12789) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunofluorescence analysis of NIH/3T3 cells using PDIA2 antibody (A12789) at dilution of 1:100. Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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In Silico Prediction of High-Resolution Hi-C Interaction Matrices
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Product Specification Sheet Protein disulfide-isomerase A2 antibody Cat # PDIH11-A Rabbit anti-Human PDIA2 antibody SIZE: 100 µg Cat # PDIM21-A Rabbit anti-Mouse PDIA2 antibody SIZE: 100 µg Protein disulfide isomerase family A member 2 Form & Storage of Antibodies (PDIA2) is a protein that in humans is encoded by the PDIA2 gene. This gene encodes a member of the disulfide isomerase Affinity pure IgG Solution (PDI) family of endoplasmic reticulum (ER) proteins that Concentration: 0.5 mg/ml Volume: 200 µl catalyze protein folding and thiol-disulfide interchange Supplied in PBS, pH 7.4 + 0.1% BSA reactions. PDI is a ubiquitous, highly conserved redox The antibody can be made available carrier free or conjugated chaperone enzyme from the thioredoxin superfamily. PDI has to HRP, Biotin, or FITC on request an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C- Lyophilized powder terminal ER-retention sequence. The protein plays a role in the Reconstitute powder in 200 µl distilled water to 0.5 mg/ml folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, Storage: and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular Short-term: 4oC for 1 month estradiol levels. Diseases associated with PDIA2 include Alpha Thalassemia-Intellectual Disability Syndrome Type 1 Long-term: at –200C or below in suitable aliquots after and Multiple Sulfatase Deficiency. reconstitution for 1 year. Do not expose to multiple free/thaw cycles or store working, diluted solutions.
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Product Data Sheet
For research purposes only, not for human use Product Data Sheet Anti-PDIA2 Antibody Catalog # Source Reactivity Applications CQA5431 Rabbit H, M, R WB, IF/IC Description Rabbit polyclonal antibody to PDIA2 Immunogen Recombinant fusion protein of human PDIA2. The exact sequence is proprietary. Purification The antibody was purified by immunogen affinity chromatography. Specificity Recognizes endogenous levels of PDIA2 protein Clonality Polyclonal Conjugation Form Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol PDIA2 Alternative Names PDIP; Protein disulfide-isomerase A2; Pancreas-specific protein disulfide isomerase; PDIp Entrez Gene 64714 (Human); 69191 (Mouse) SwissProt Q13087 (Human); D3Z6P0 (Mouse) Storage/Stability Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish COHESION BIOSCIENCES LIMITED WEB ORDER SUPPORT CUSTOM www.cohesionbio.com [email protected] [email protected] [email protected] For research purposes only, not for human use Product Data Sheet Western blot analysis of PDIA2 expression in U87MG (A), mouse liver (B), rat liver (C) whole cell lysates. Immunofluorescent analysis of PDIA2 staining in NIH3T3 cells.
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Datasheet Blank Template
SANTA CRUZ BIOTECHNOLOGY, INC. PDIA2 siRNA (h): sc-93245 BACKGROUND STORAGE AND RESUSPENSION Oxidoreductase-protein disulfide isomerase (PDI) is a homodimer that catalyzes Store lyophilized siRNA duplex at -20° C with desiccant. Stable for at least thiol-disulfide exchange, mediates folding of newly synthesized proteins and one year from the date of shipment. Once resuspended, store at -20° C, functions as a molecular chaperone. PDIA2 (protein disulfide-isomerase A2), avoid contact with RNAses and repeated freeze thaw cycles. also known as PDIR (protein disulfide isomerase-related protein), PDI, PDA2 or Resuspend lyophilized siRNA duplex in 330 µl of the RNAse-free water PDIP (protein disulfide isomerase, pancreatic), is a 525 amino acid endoplasmic provided. Resuspension of the siRNA duplex in 330 µl of RNAse-free water reticulum lumen protein that is highly expressed in the pancreas. Belonging to makes a 10 µM solution in a 10 µM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM the protein disulfide isomerase family, PDIA2 contains two thioredoxin (CXXC) EDTA buffered solution. domains,which mediate substrate-specific isomerases and chaperone PDIA2’s redox activity. PDIA2 is suggested to function as an enzyme that catalyzes the APPLICATIONS rearrangement of -S-S- bonds in proteins. PDIA2 is encoded by a gene located on human chromosome 16, which encodes over 900 genes and comprises PDIA2 siRNA (h) is recommended for the inhibition of PDIA2 expression in nearly 3% of the human genome. human cells. REFERENCES SUPPORT REAGENTS 1. Hayano, T. and Kikuchi, M. 1995. Molecular cloning of the cDNA encoding For optimal siRNA transfection efficiency, Santa Cruz Biotechnology’s a novel protein disulfide isomerase-related protein (PDIR).
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