LCGC LCGC NORTH AMERICA

Corporate Capabilities

— 2020 Corporate

CapabilitiesA Guide to Leading Separation Science Suppliers

2020 Application Notes ♦ See page 52 2 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2015 www.chromatographyonline.com

Experience New Benchmarks New Nexera UHPLC series Sets Industry Standard for Intelligence, Effi ciency and Design Incorporating artifi cial intelligence as Analytical Intelligence • Intelligence, utilizing the Internet of Things (IoT), and Real-time mobile phase monitor • Auto-diagnostics/Auto-recovery (fl ow anomalies) featuring the most advanced performance features • Auto fl ow-ramping available, Shimadzu’s new compact Nexera UHPLC Effi ciency Series enables smarter, more effi cient workfl ows, • Highest cooling effi ciency higher productivity, and maximum reliability. • Fastest sampling speeds • Co-injection automation www.NexeraSeries.com Design • Space-saving system design Shimadzu Scientifi c Instruments, 7102 Riverwood Drive, • Enormous sample capacity Columbia, MD 21046, 800-477-1227 • Enhanced CMD

magentablackcyanyellow 36619100386_SHIMADZU_Pgxx_FP_1-1.pgs 09.25.2019 00:45 ADVANSTAR_PDF/X-1a www.chromatographyonline.com DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 3 4 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com

Corporate Capabilities 2020

Corporate Profiles

Advanced Chemistry Development, MACHERY-NAGEL 27 Showdex/Showa Denko Inc. (ACD/Labs) 6 America, Inc. 44 Markes International 28 Advanced Materials Technology 8 SilcoTek Corporation 45 Merlin Instrument Company 29 Antec Scientific 9 Thermo Fisher Scientific 46 MicroSolv Technology Corporation 3 0 Biotage, LLC 10 Tosoh Bioscience LLC 47 Neta Scientific, Inc. 31 Biotech USA LLC 11 UCT, Inc. 48 Optimize Technologies, Inc. 32 CEM Corporation 12 Valvo Instruments Co., Inc. Parker Hannifin 33 (VICI)  49 Chem Service, Inc. 14 Peak Scientific 34 Wyatt Technology 50 CDS Analytical 16 PharmaFluidics 35 YMC America, Inc. 51 Chiral Technologies, Inc. 17 Photonis Scientific, Inc. 36 Ellutia 18 Pickering Labratories, Inc. 37 Application Notes Fritsch Milling & Sizing, Inc. 19 Postnova Analytics Inc. 38 Restek Corporation 52 IDEX Health & Science 20 Proton OnSite 39 Hamilton Company, Inc. 54 GERSTEL, Inc. 22 Polymer Standards Service USA Inc Showdex/Showa Denko Imtakt USA 23 PSSUSA, Inc. 40 America, Inc. 55

KIN-TEK Analytical, Inc. 24 Restek  Corporation 41 MilliporeSigma 56

LECO Corporation 25 SepSolve Analytical Ltd. 42 Pickering Laboratories, Inc. 57

LabVantage Solutions, Inc. 26 Shimadzu Scientific Instruments 43 CDS Analytical 58 www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 5

MANUSCRIPTS: For manuscript preparation guidelines, see chromatographyonline. 485F US Highway One South, MARKETING/OPERATIONS com/lcgc-author-guidelines, or call The Editor, (732) 596-0276. LCGC welcomes unso- Suite 210 Marketing Manager licited articles, manuscripts, photographs, illustrations, and other materials but can- Iselin, NJ 08830 Brianne Pangaro not be held responsible for their safekeeping or return. Every precaution is taken to (732) 596-0276 Fax: (732) 647-1235 [email protected] ensure accuracy, but LCGC cannot accept responsibility for the accuracy of informa- tion supplied herein or for any opinion expressed. C.A.S.T. Data and List Information Melissa Stillwell PUBLISHING/SALES [email protected] SUBSCRIPTIONS: For subscription and circulation information: LCGC, P.O. Box 457, Cranbury, NJ 08512-0457, or call (888) 527-7008 (7:00 a.m.–6:00 p.m. Vice President/Group Publisher Reprints central time). International customers should call +1-218-740-6477. Delivery of Michael J. Tessalone Alexandra Rockenstein LCGC outside the United States is 14 days after printing. For single and back [email protected] [email protected] issues, call (800) 598-6008 or (218) 740-6480. (LCGC Europe and LCGC Asia Associate Publisher Audience Development Manager Pacific are available free of charge to users and specifiers of chromatographic Edward Fantuzzi Jessica Stariha equipment in Western Europe and Asia and Australia, respectively.) [email protected] [email protected] Sales Manager CHANGE OF ADDRESS: Send change of address to LCGC, P.O. Box 457, Brianne Molnar CORPORATE Cranbury, NJ 08512-0457; alternately, send change via e-mail to [email protected] [email protected] or go to the following URL: Chairman & Founder Senior Director, Digital Media http://mmhpubs.mmhgroup.com/Welcome.aspx?pubid=LCGC Mike Hennessy Sr Allow four to six weeks for change. PUBLICATIONS MAIL AGREEMENT Michael Kushner [email protected] No. 40612608. Return all undeliverable Canadian addresses Vice Chairman to: IMEX Global Solutions, P.O. Box 25542, London, ON, N6C Jack Lepping 6B2, CANADA. Canadian GST number: R-124213133RT001. EDITORIAL President & CEO Mike Hennessy Jr C.A.S.T. DATA AND LIST INFORMATION: Contact Melissa Stillwell, Editorial Director Chief Strategy Officer & tel. (218) 740-6831, e-mail [email protected]. Laura Bush [email protected] President, Agency Services REPRINTS: Contact Michael J. Tessalone, George Glatcz e-mail: [email protected] Managing Editor INTERNATIONAL LICENSING: Contact Kim Scaffidi, John Chasse Chief Financial Officer e-mail: [email protected] [email protected] Neil Glasser, CPA/CFE Senior Technical Editor Executive Vice President, CUSTOMER INQUIRIES: Customer inquiries can be forwarded directly to MJH Jerome Workman Operations Life Sciences, Attn: Subscriptions, 2 Clarke Drive, Suite 100, Cranbury, NJ [email protected] Tom Tolvé 08512; e-mail: [email protected] Associate Editor Senior Vice President, Content Cindy Delonas Silas Inman ecycled R Pa % p 0 e r [email protected]

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0 a % Art Director & Enterprise Systems W P r os e John Moricone t Consum Gwendolyn Salas [email protected] Senior Vice President, © 2019 MultiMedia Pharma Sciences, LLC. All rights reserved. No part of this publi- Graphic Designer Development & cation may be reproduced or transmitted in any form or by any means, electronic or Courtney Soden Enterprise Systems mechanical including by photocopy, recording, or information storage and retrieval [email protected] John Paul Uva without permission in writing from the publisher. Authorization to photocopy items for internal/educational or personal use, or the internal/educational or personal Senior Vice President, Audience use of specific clients is granted by MultiMedia Pharma Sciences, LLC. for libraries CONTENT MARKETING Generation & Product Fulfillment and other users registered with the Copyright Clearance Center, 222 Rosewood Dr., Joy Puzzo Danvers, MA 01923, (978) 750-8400, fax (978) 646-8700, or visit http://www.copyright. Custom Content Writer Vice President, Human com online. For uses beyond those listed above, please direct your written request Allissa Marrapodi Resources & Administration to Permission Dept. email: [email protected] [email protected] Shari Lundenberg MultiMedia Pharma Sciences, LLC. provides certain customer contact data (such as Webcast Operations Manager Vice President, Business customer’s name, addresses, phone numbers, and e-mail addresses) to third parties Kristen Moore Intelligence who wish to promote relevant products, services, and other opportunities that may [email protected] be of interest to you. If you do not want MultiMedia Pharma Sciences, LLC. to make Chris Hennessy Project Manager your contact information available to third parties for marketing purposes, simply Vice President, Corporate Vania Oliveira call toll-free (866) 529-2922 between the hours of 7:30 a.m. and 5 p.m. CST and a cus- Branding & B2B Marketing [email protected] tomer service representative will assist you in removing your name from MultiMedia Amy Erdman Pharma Sciences, LLC. lists. Outside the U.S., please phone (218) 740-6477. Digital Production Manager Executive Creative Director, LCGC North America does not verify any claims or other information a­ ppearing in Sabina Advani Creative Services any of the advertisements contained in the publication, and cannot take responsi- [email protected] Jeff Brown bility for any losses or other damages incurred by readers in reliance of such content. Managing Editor, Special Projects To subscribe, call toll-free (888) 527-7008. Outside the U.S. call (218) 740-6477. Kaylynn Chiarello-Ebner [email protected] 6 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Advanced Chemistry Development, Inc. (ACD/Labs)

conditions, and estimate retention times. ⦁⦁ ACD/AutoChrom–comprehensive LC method development and optimization software that supports a Quality by Design (QbD) approach to method design, execution, and reporting. Create a method strategy, perform screening runs, optimize based on the complete Design of Experiments (DoE) space, and implement robustness testing to achieve the optimal method. Directly operate Agilent ChemStation and Waters Empower instruments. ⦁⦁ ACD/MS Workbook Suite–an advanced set of software tools for detecting, identifying, and characterizing LC-MS & GC-MS components. Import datafiles from virtually all major instrument vendors, Company Description extract all relevant chromatographic ACD/Labs is a leading provider of scientific software to R&D components, predict ion fragmentation, organizations. We help our customers assemble digitized screen parameters against spectral and analytical, structural, and molecular information for effective structural libraries, and archive projects in decision-making, problem-solving, and product lifecycle accessible databases. control. Our enterprise technologies enable automation of ⦁⦁ Luminata–decision support molecular characterization, product development, and facilitate software to accelerate chemistry, chemically intelligent knowledge management, data analytics, manufacturing, and controls (CMC) and collaboration—within organizations and between partners. product development. Implement a ACD/Labs provides worldwide sales and support, and bring single vendor-neutral, multi-technique 25 years of experience and success helping organizations in- platform to effectively manage all CMC novate and create efficiencies in their workflows. process scheme, impurity control, drug Advanced Chemistry Development, Inc. stability, formulation development, and (ACD/Labs) Chief Chromatographic Techniques Supported batch genealogy information, according 8 King Street East, Suite 107 ⦁⦁ HPLC to QbD regulatory requirements. Toronto, Ontario, Canada ⦁⦁ UHPLC M5C 1B5 ⦁⦁ LC–MS ⦁⦁ LC–MS/MS TELEPHONE +1 4163683435 ⦁⦁ GC–MS E-MAIL Markets Served [email protected] ACD/Labs solutions are used in a variety of industries, including WEB SITE pharma and biotech, fine chemicals, consumer packaged goods, acdlabs.com agrochemicals, petrochemicals, academia, and government.

YEAR FOUNDED 1994 Major Products/Services ⦁⦁ ACD/Method Selection Suite–LC & GC method simulation software that combines physicochemical property predictions with method development tools to rationally optimize key separation parameters, define better starting Enable Robust Methods, Ensure Data Integrity

Software to streamline method development using in silico optimization ACD/Method Selection Suite

Generate Robust Methods in silico Optimize pH, gradient, temperature, & more through simulation

4 5 35º 40º

6 7 15º 25º Predict the Impact Column pH Temp of Method Changes Visualize the effect of varying methods Minimize Experiments without added Build optimized experiments methods with minimal injections

Follow Quality by Design (QbD) Principles QbD Empower Data Integrity Ensure data Strategy through Databasing management Easily database all complies with QbD method development recommendations outcomes to preserve critical knowledge

www.acdlabs.com/methdev

A trademark of Advanced Chemistry Development, Inc. (ACD/Labs) 8 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Advanced Materials Technology

Major Products/Services At Advanced Materials Technology we combine our collective years of silica engineering experience to deliver HALO® superficially porous particle (U)HPLC col- umns. Unlike other companies, we are solely focused on the development of this technology which delivers highly efficient, robust, and reliable separations to solve customers’ analytical challenges.

Facility Advanced Materials Technology, located in Wilmington, Delaware, USA, consists of R&D, Applications, QA/QC, Manufacturing, and Marketing departments. Chemists and engineers operate in fully equipped state of the art laboratories. AMT is a company of innovators that continues to grow and Company Description deliver enabling separation materials Advanced Materials Technology, founded in 2005, is an to market. Our incredible team is our innovative product development and manufacturing company in greatest resource. the separation sciences focused on ground-breaking technology to solve the unsolvable! From pioneering Fused-Core® technology with the first commercially available sub 3-µm superficially porous particle, the HALO® product line has revolutionized HPLC separations for small molecule and large molecule separations. With over 120 years of ingenuity in design and manufacturing, Advanced Materials Technology continues to be a leader in novel superficially porous particle development enabling high efficiency and reliability for scientists involved in both small and large molecule separations. Advanced Materials Technology Chief Chromatographic Techniques Supported 3521 Silverside Road ⦁ Suite 1-K, Quillen Building ⦁ Reverse-phase (U)HPLC separations Wilmington, DE 19810 ⦁⦁ HILIC separations ⦁⦁ Small molecule (U)HPLC separations E-MAIL ⦁⦁ Large molecule (U)HPLC separations info@advanced-materials-tech. com Markets Served WEB SITE ⦁⦁ Pharmaceutical fused-core.com ⦁⦁ Bio-Pharmaceutical YEAR FOUNDED ⦁⦁ Food and Beverage 2005 ⦁⦁ Environmental ⦁⦁ Industrial ⦁⦁ Cannabis www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 9

Electrochemical Reactors Antec Scientific for MS and Synthesis With the introduction of the ROXY™ EC system, REDOX reactions can be monitored directly by mass spectrometry (MS). The on-line coupling of electrochemical reactors with MS (EC–MS) has found many application areas from proteomics, lipidomics, drug metabolism, drug stability testing, to environmental degradation. Another emerging application of Antec’s flow and batch reactors is in Flow Chemistry for rapid synthesis of drugs, metabolites, and degradants (reference materials).

SenCell™ for Detection Proprietary Adjustable Spacer Technology (AST) for highest sensitivity (US Patent 9,310,330)

µ-PrepCell for Reactions Redox reactions upfront MS: disulfide bond reduction in proteins, mimicking drug metabolism, drug stability testing/ degradation

Company Description SynthesisCell™ for Synthesis For over 29 years, Antec Scientific has been the world Batch reactor for rapid synthesis of mg leading supplier of analytical instrumentation based on quantities of products electrochemistry (EC). Antec’s line of instruments include electrochemical detectors (ECD), analyzers built on HPLC- ECD, and electrochemical reactors for use with MS (EC–MS) and synthesis.

Electrochemical Detectors for HPLC The DECADE™ line of ECDs (Elite™ and Lite™) have become the benchmark in detection, and can be used with any third party (U)HPLC system. Different flow cells are available to cover a broad range of applications from the analysis of antibiotics to Antec Scientific One Boston Place, sugars or pharmaceuticals. 201 Washington Street, Boston, MA 02108 Dedicated Analyzers The ALEXYS™ Analyzers based on HPLC with ECD are turnkey TELEPHONE instruments built for highest performance and to ensure ease- (888) 572-0012 in-use. Guaranteed applications have been developed with E-MAIL the ALEXYS Neurotransmitter Analyzer for sensitive analysis in [email protected] microdialysates and brain tissue. Other application areas are WEB SITE in clinical/diagnostics, food/beverage, environmental, and www.antecscientific.com drugs/pharmaceuticals analysis.

YEAR FOUNDED 1990, since 2010 in USA 10 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Biotage, LLC

Markets Served Separation, synthesis, sample cleanup, extraction, and concentration are important parts of our customers’ workflows. Through our broad selection of innovative products, Biotage helps laboratories streamline these workflows in markets including: environmental, food, agricultural, chemical, petrochemical, forensic, clinical, toxicological, pharmaceutical, and biotechnology.

Major Products/Services Biotage aims to be a natural part of our customers’ workflow. Thanks to our close relationship with end users and our market presence, we are able to identify customer needs and respond to future challenges by introducing new products and solutions. Biotage covers Company Description the entire value chain from research Biotage is a global company offering innovative and effective and development through production, solutions for separation and sample preparation within sales, and service. Our product offerings organic and analytical chemistry, as well as for industrial include: manual and automated applications. Through our broad selection of state-of-the-art extractors, automated purification systems, products, Biotage helps customers streamline their workflows microwave-assisted synthesis, benchtop and reduce the impact of their activities on the environment. nitrogen evaporation systems, peptide By making their work easier, increasing their productivity, and synthesis, and a wide-range of sample offering them more sustainable solutions, Biotage is laying preparation consumables. the foundation for better, more cost-effective manufacturing Biotage, LLC for customers all around the world. Facilities 10430 Harris Oaks Blvd. Two strategic acquisitions have contributed to the recent Global headquarters in Sweden. Suite C growth of Biotage. The acquisition of Horizon Technology Inc. USA headquarters in North Carolina. Charlotte, NC 28269 strengthened the company’s product portfolio and technical Applications laboratories in Sweden and TELEPHONE expertise in environmental and food safety applications. The USA. Sales offices in Sweden, USA, Korea, (704) 654-654-4900 acquisition of PhyNexus Inc. fueled the company’s growth Japan, China, and India. FAX within the chemical separation market, specifically addressing (704) 654-4917 the rapidly growing field of biomolecules.

E-MAIL [email protected] Chief Chromatographic Techniques Supported ⦁⦁ Sample Preparation and Cleanup WEB SITE ⦁⦁ Solid-Phase Extraction www.biotage.com ⦁⦁ Supported Liquid Extraction NUMBER OF EMPLOYEES ⦁⦁ Evaporation and Concentration (USA): 130 ⦁⦁ Protein Precipitation (Elsewhere): 320 ⦁⦁ Flash Chromatography YEAR FOUNDED ⦁⦁ GC 1989 ⦁⦁ GC–MS ⦁⦁ HPLC ⦁⦁ LC–MS www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 11 Biotech USA LLC

Markets Served ⦁⦁ Instrument Developers ⦁⦁ OEM customers ⦁⦁ Distributors ⦁⦁ End users

Major Products/Services ⦁⦁ Degassing & Debubbling ⦁⦁ DEGASi Stand Alone Degassers ⦁⦁ Detectors ⦁⦁ Fittings & Connectors ⦁⦁ HPLC/UHPLC Columns ⦁⦁ Column Hardware ⦁⦁ HPLC Filters & Frits ⦁⦁ Knitted Delay/reaction Coils ⦁⦁ Level Sensor ⦁⦁ Smart Saver – USB Solvent Recycler ⦁⦁ Tubing ⦁⦁ Pumps ⦁⦁ Valves ⦁⦁ OEM Solutions ⦁⦁ Service and Support

Facility Company Description Biotech AB (HQ) is situated on the Onsala Biotech are supplying innovative laboratory products to instru- peninsula on the Swedish Westcoast near ment manufacturers, distributors, and end-users around the Gothenburg City and Landvetter Interna- world. As a Global Premium Distributor for IDEX, we have tional Airport. We operate globally and special know-how about their assortment, and can provide have subsidiaries in US and Japan to give you with the right mix of products for a successful fluidic you the best service and support. Biotech USA LLC system. PO Box 18796 Minneapolis, MN 55418 No troubles with bubbles We specialize in chromatography and finding solutions to TELEPHONE issues with gases and bubbles in fluidic systems. By using (612) 703-5718 our advanced degassers, the laboratory can optimize the per-

FAX formance of its analytical instruments and chromatographic (866) 682-5816 equipment. Our degassing solutions save the industry both time and money by avoiding production disruptions caused E-MAIL by bubbles in the fluidic lines. [email protected] WEB SITE Chief Chromatographic Techniques Supported www.biotechfluidics.com ⦁⦁ HPLC ⦁⦁ UHPLC ⦁⦁ GPC ⦁⦁ Preparative HPLC ⦁⦁ SFC ⦁⦁ Ion Chromatography 12 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com CEM Corporation

Major Products/Services The CEM EDGE is a fully automated extraction system that performs solvent addition, extraction, filtration, and system wash in a fraction of the time of other techniques. The EDGE is a faster and more efficient alternative to QuEChERS for the extraction of pesticides from food matrices. The EDGE is also an ideal system for the extraction of pesticides, cannabinoids, mycotoxins, and terpenes from cannabis and hemp. All cannabis extractions can be performed on a single EDGE system, in series, to achieve the throughput necessary for today’s fast paced cannabis labs. The EDGE also supports extraction of plastics, soils, and more.

Facility Company Description CEM’s global headquarters, research labo- CEM Corporation is a leading global company specializing ratories, and manufacturing facilities are in scientific solutions for critical laboratory applications. We located in Matthews, North Carolina. The design and manufacture systems for analytical laboratories, company has subsidiaries in the United bioscience applications, life sciences, and processing plants Kingdom, Ireland, Germany, Italy, France, worldwide. Our product portfolio includes innovative and Japan, as well as more than 50 dis- instrumentation for sample preparation for elemental or tributors worldwide. chromatographic analysis, chemical synthesis, peptide synthesis, and other applications.

CEM Corporation Chief Chromatographic Techniques Supported PO Box 200, ⦁⦁ LC Matthews, NC 28106 ⦁⦁ LC–MS TELEPHONE ⦁⦁ GC (704) 821-7015 ⦁⦁ GC–MS (800) 726-3331

FAX Markets Served (704) 821-7894 CEM instrumentation is used by chemists, scientists, and

E-MAIL technicians in private industry, as well as leading universities, [email protected] analytical laboratories, and research facilities around the world. We support numerous markets, including the WEB SITE pharmaceutical, chemical, environmental, applied materials, www.cem.com food, and petroleum industries, among others. NUMBER OF EMPLOYEES USA: 260 Elsewhere: 65

YEAR FOUNDED 1978 EDGE

Automated Extraction System

Meet the new extraction system that yields exceptional recoveries in as little as 5 minutes.

Foods Plastics Environmental Cannabis

cem.com/EDGE 14 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Chem Service, Inc.

agencies; food quality control; agricultural testing and research; life science endeavors; environmental control and remediation; college and university work; and the manufacture of chemicals, pulp and paper, and alcoholic beverages.

Major Products/Services ⦁⦁ Pesticide & Metabolite standards ⦁⦁ Cannabis pesticide standards ⦁⦁ Residue solvent standards ⦁⦁ Terpene standards ⦁⦁ Flame retardants/PBDE ⦁⦁ EPA, USP, ISO, and DIN standards ⦁⦁ Explosive residues ⦁⦁ PCB congeners ⦁⦁ Petroleum hydrocarbons ⦁⦁ Fatty acid methyl esters ⦁⦁ High-purity inorganics Company Description ⦁⦁ Surfactants; Anti-oxidants Established in 1962, Chem Service is accredited for ISO 17034 ⦁⦁ Ultraviolet inhibitors and ISO 17025 and registered and certified to the ISO 9001 ⦁⦁ Plasticizers; Biological stains Quality Management System for the design, development, ⦁⦁ Phthalate standards production, distribution, and servicing of organic neat and ⦁⦁ Polynuclear aromatics synthetic reference materials. ⦁⦁ Vitamins Chem Service, Inc., produces high-purity chemicals for use as ⦁⦁ Carbohydrates standards reference materials and for other laboratory purposes. More than ⦁⦁ Forensic standards 95% of their standards grade materials have a certified purity of ⦁⦁ Personal care product standards 98.0% or greater. Standards-grade chemicals are clearly labeled with an expiration date that is based on years of experience in Facility  handling and testing. Products are packaged in small quantities Chem Service’s headquarters are located in Chem Service, Inc. to minimize storage, waste, and disposal requirements. Organic West Chester, PA. A global network of dis- 660 Tower Lane, and inorganic chemicals, solutions, and mixtures are available to tributors for sales outside the U.S. is available West Chester, PA 19381 meet a wide range of specialized laboratory needs. for all of our products. TELEPHONE (800) 452-9994 Chief Analytical Techniques Supported

FAX ⦁⦁ GC–FID (610) 692-8729 ⦁⦁ GC–ECD ⦁⦁ GC–TCD E-MAIL ⦁⦁ GC–MS [email protected] ⦁⦁ HPLC WEB SITE ⦁⦁ FT–IR www.chemservice.com ⦁⦁ UHPLC–MS YEAR FOUNDED ⦁⦁ DSC 1962 ⦁⦁ TGA

Markets Served Chem Service has thousands of worldwide customers in a wide range of sectors, including government and regulatory Analytical Standards and Certified Reference Standards

Certified Reference Standards Established in 1962, we pride ourselves in setting the standard for high purity chemicals. Chem Service is accredited to ISO 17034:2016, ISO/IEC 17025:2005, and registered and certified to the ISO 9001:2015 Quality Management System for the design, development, production, distribution, and servicing of organic neat and synthetic reference materials.

• Setting the standard for over 55 years. • More than 14,000 products to choose from and adding new products daily. • Custom Mixtures, Solutions, and Custom Synthesis available.

chemservice.comchemservice.com • | [email protected] [email protected] • 1-800-452-9994 | 1-800-452-9994 16 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com CDS Analytical

Major Products/Services Sixth-generation Pyroprobe™ with DISC technology offers easy sample loading, unparalleled reproducibility, hybrid Autosampler and superior resolution. The Photoprobe is a new module added to the Pyroprobe™ line for weather degradation studies. The 2nd generation stand-alone Thermal Desorption autosampler supports the Peltier electric cooling to handle C2 to C44 level VOCs. The 7000C is the industry’s first Purge and Trap concentrator that is integrated with PAL System. The Dynamic Headspace Module is capable of thermally extracting the sample at 300 °C. The Empore™ SPE disks, cartridges and 96-well plate is the leading brand in Solid Phase Extraction consumables.

Company Description Facility CDS Analytical has celebrated its 50th anniversary. CDS CDS Analytical’s facility locates in Oxford, Analytical has been offering a full range of GC Sample Pennsylvania. CDS products passed Introduction methods, including Pyrolysis, Thermal Desorption, various safety regulations, including CE Purge and Trap, and Dynamic Headspace. As of today, CDS and NRTL. The phase 2 construction has Analytical gathers world-class engineers and analytical chemists been completed in 2019. With this newly working together to continue the “Made in USA” legend. finished cutting edge GMP-compliant clean room facility, CDS Analytical is CDS Analytical acquired the 3M™ Empore™ line in 2019. The bringing its production compliance to the Empore™ brand solid phase extraction (SPE) membranes has next level. been widely used in environmental, food, biopharmaceutical, and academic fields. With this acquisition, CDS Analytical has CDS Analytical further strengthened its footprint in the sample preparation 465 Limestone Road, market with enhanced offerings in the consumables. Oxford, PA 19363

TELEPHONE Chief Chromatographic Techniques Supported (800) 541-6593 ⦁⦁ Analytical Pyrolysis

FAX ⦁⦁ Purge and Trap (610) 932-4158 ⦁⦁ Thermal Desorption ⦁⦁ Dynamic Headspace E-MAIL [email protected] ⦁⦁ Solid-Phase Extraction WEB SITE Markets Served www.cdsanalytical.com CDS Analytical products have been serving the following markets: YEAR FOUNDED Polymer, Rubber, Environmental, Aerospace, Automobile, Year 1969 Chemical Weapon, Energy, Food and Flavor, Forensics, Paper &

Ink, Pharmaceuticals, Semiconductor, Textiles, and Tobacco. Innovation www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 17 Chiral Technologies, Inc.

⦁⦁ Columns packed with sub-2 and 3-µm particles are available for rapid analyses of drug candidates. ⦁⦁ Bulk chiral stationary phases are supplied for process-scale chiral chromatography. ⦁⦁ The company also provides analytical method development and custom separation services.

Facility Chiral Technologies’ headquarters, including a state-of-the-art technical center, are located in West Chester, PA.

Company Description  Chiral Technologies, Inc. is a subsidiary of Daicel Corporation, the global market leader in enantioselective chromatography, offering the most extensive portfolio of chiral stationary phases available on the market. Enantioselective chromatography is essential to accelerate drug discovery and development processes. Scientists in the pharmaceutical, agricultural and food industries, universities, and government agencies rely on our expertise in enantioselective chromatography to separate chiral molecules into single Chiral Technologies, Inc. enantiomers in research and development departments, and 800 North Five Points Road QC laboratories to initiate pre-clinical trials. West Chester, PA 19380 TELEPHONE Chief Chromatographic Techniques Supported (610) 594-2100 ⦁⦁ High-Performance Liquid Chromatography (HPLC) or 800 6CHIRAL ⦁⦁ Ultra-High Performance Liquid Chromatography (UHPLC) ⦁⦁ Supercritical Fluid Chromatography (SFC) FAX (610) 594-2325 ⦁⦁ Simulated Moving Bed (SMB) technique E-MAIL Markets Served [email protected] Chiral Technologies serves the North and Latin American WEB SITE markets through Chiral Technologies Inc. (West Chester, PA), www.chiraltech.com the European market through Chiral Technologies Europe SAS NUMBER OF EMPLOYEES (Illkirch, France), and the Asian market through Daicel Chiral 25 Technologies India and Daicel Chiral Technologies China, all wholly owned subsidiaries of Daicel Corporation. YEAR FOUNDED 1990 Major Products/Services Daicel chiral columns: ⦁⦁ Revolutionary immobilized CSPs 18 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Ellutia

⦁⦁ Agro-chemical ⦁⦁ Cosmetics ⦁⦁ Toy production ⦁⦁ High purity gas ⦁⦁ Natural gas ⦁⦁ Environmental testing

Major Products/Services ⦁⦁ 200 Series GC: A compact and versatile single channel gas chromatograph ⦁⦁ 500 Series GC: A high-speed GC using resistively heated columns for ultra fast analysis cycle times ⦁⦁ High Purity Gas Analyzer: A rack- mounted GC for detecting impurities in gas to extremely low levels ⦁⦁ 800 Series TEA: Nitro, nitroso, and nitrogen detector that can be interfaced with almost any GC ⦁⦁ Ellution Chromatography Data Station: Software for collecting and processing data from a GC ⦁⦁ 7000 Flowmeter: A compact handheld flowmeter for accurately setting flows Company Description on a GC Ellutia offers a range of gas chromatographs, GC accessories, software, and consumables. Ellutia’s instruments are designed Facilities and manufactured in the UK at the company headquarters. Ellutia’s headquarters are located just Ellutia Ltd The instruments are designed to be more compact and have outside Cambridge in the UK. This facility Colston House greater energy efficiency, while still delivering industry stan- hosts the main research and development 200 Lancaster Way Bs Pk dard analytical performance. Ellutia works closely with its labs and is where all of the instruments Ely, Cambs customers, and regularly produces fully customized systems are manufactured and tested. Outside CB63NX tailored to their individual requirements rather than just deliv- of the UK, Ellutia also has an office in UK ering off the shelf products. It is this working relationship with Germany and has recently opened a new

TELEPHONE customers and the flexibility to adapt that sets Ellutia apart dedicated sales and service organization +44 1353 669916 from other GC manufacturers. in the US.

FAX +44 1353 669917 Chief Chromatographic Technique Supported ⦁⦁ Gas chromatography E-MAIL [email protected] Markets Served WEB SITE Ellutia’s products can be found in a wide range of industries, www.ellutia.com including

NUMBER OF EMPLOYEES ⦁⦁ Brewing and malting 17 ⦁⦁ Food testing ⦁⦁ Packaging testing YEAR FOUNDED 1994 ⦁⦁ Permeation testing ⦁⦁ Education ⦁⦁ Forensic laboratories www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 19 Fritsch Milling & Sizing, Inc.

Company Description FRITSCH is an internationally recognized manufacturer of application-oriented laboratory instruments for sample preparation and particle sizing.

Chief Chromatographic Techniques Supported Laboratory Mills for optimal sample preparation of chromatographic techniques

Markets Served Worldwide in the following industries: Mining, Construction Fritsch Milling materials, Biology, Chemistry, Mechanochemistry, & Sizing, Inc. Agriculture and forestry, Geology/mineralogy, Ceramics/glass 57 Grant Drive Suite G, industry, Plastics/textiles, Foodstuffs, Metallurgy, Environment, Pittsboro, NC 27312 Pharmaceuticals

TELEPHONE (919) 229-0599 Major Products/Services Particle size reduction, milling, grinding, and analysis of E-MAIL particle size and shape. [email protected]

WEB SITE Facility www.fritsch-us.com USA headquarters and applications laboratory in North NUMBER OF EMPLOYEES Carolina. Global headquarters in Germany. Sales offices in 100+ worldwide China, Asia Pacific, Russia, and France.

YEAR FOUNDED 1920-Fritsch GmbH 20 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com IDEX Health & Science

Markets Served Life science, cell isolation, clinical chemistry, diagnostics, droplet-based applications, he- matology, immunoassay, liquid biopsy, liquid chromatography, mass spectrometry, biopro- cessing, multiplexed molecular assays, next generation sequencing, organ-on-a-chip, point of care, target enrichment workflows.

Major Products/Services We develop, engineer, and manufacture optofluidic components and sub-assem- blies for life sciences and healthcare. Column hardware, degassers, fluidic con- nections, manifolds, pumps, RI detectors, sensors, valves, optical filters, cameras, filter wheels, imaging objectives, laser light sources, sub systems, bioprocessing, mi- crofluidic consumables, sample-to-answer Company Description solutions, supporting labware. IDEX Health & Science is the global leader in life science fluidics, microfluidics, and optics, offering a three-fold advan- Facility tage to customers by bringing optofluidic paths to life with Fifteen locations across the globe strategic partnerships, breakthrough solutions, and proven expertise. As one of the few companies in the world with component, sub-system, and application level experts, IDEX Health & Science helps instrument developers solve the most demanding fluidic and optical challenges in a wide array of applications. At IDEX Health & Science, we believe partner- ship will change the way the world innovates, leading to new technologies that improve our health, protect our planet, and IDEX Health & Science enrich our lives. 600 Park Ct. Rohnert Park, CA 94928 Chief Chromatographic Techniques Supported TELEPHONE ⦁⦁ HPLC (707) 588-2000 ⦁⦁ UHPLC

E-MAIL ⦁⦁ UPLC [email protected] ⦁⦁ Mass Spectrometry ⦁⦁ SFC WEB SITE www.idex-hs.com ⦁⦁ NanoHPLC ⦁⦁ Microfluidics NUMBER OF EMPLOYEES 1200

YEAR FOUNDED 2008 HPLC Degassing Control Revolutionized Take Control of Your HPLC Degassing Methods with a High Efficiency Constant Performance Film Degasser

In an unprecedented leap in degassing technology, our experts have pushed the limits of what’s possible when it comes to high performance liquid chromatography. Our new Film Degasser, in a small form factor, uses a patent-pending algorithm that allows you to select an optimal degassing efficiency for any HPLC method, for consistent operation. Utilizing a high-efficiency membrane and unique bioinert flow channel design, it delivers the highest efficiency and lowest fluidic resistance in the industry.

Learn how to take control of your degassing at www.idex-hs.com/FilmDegasser

IDX2972_FilmDegasser-PrintAd-LCGC_R1-FNL.indd 1 10/21/19 2:03 PM 22 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com

⦁⦁ MultiPurpose Sampler MPS: The GERSTEL GERSTEL, Inc. MPS is a highly reliable robotic automa- tion platform, which provides sample handing, preparation and introduction. A large variety of techniques can be performed by the MPS. These are listed below. Company Description ⦁⦁ Automated SPE: Independent of LC–MS Gerstel is a world leader in ana- or GC–MS or combined with sample in- lytical system automation for GC– troduction. Based on standard cartridges. MS and LC–MS instrumentation. ⦁⦁ Automated DPX: Dispersive SPE tech- Gerstel’s automation for sample nique for fast, efficient extraction and preparation, sample introduction, concentration of analytes. and standalone WorkStations is ⦁⦁ Dynamic Headspace (DHS): Concen- compatible with all manufactur- trates VOCs from liquids or solids. ers of analytical technology. Total Ultralow detection limits. DHS Large system solutions from Gerstel sample containers up to 1 L. provide unique advantages by en- ⦁⦁ Thermal Desorption Unit (TDU): Highly hancing productivity and system flexible, automated analysis of up to stability and by improving quality 240 samples. Performs thermal of results while reducing solvent desorption/extraction and pyrolysis. use and overall cost. ⦁⦁ Thermal Desorption System (TDS): Ultra-low detection limits for VOCs and Chief Chromatographic SVOCs up to n-C40+. Techniques Supported ⦁⦁ Twister® Stir Bar Sorptive Extraction ⦁⦁ Autosamplers for GC–MS and (SBSE): Ultratrace level solvent-less LC–MS determination of organic compounds ⦁⦁ Automated sample preparation for GC–MS and LC–MS in aqueous matrices. Up to 1000 times ⦁⦁ Sample Preparation WorkStation for making standards, more sensitive than SPME. preparing dilution series, derivatization, and more ⦁⦁ Cooled Injection System (CIS): Univer- ⦁⦁ Automated solid-phase extraction (SPE) sal PTV inlet for optimized GC perfor- ⦁⦁ Dispersive SPE (DPX) mance. High temperature version up to ⦁⦁ Solid Phase Micro-Extraction (SPME) and Headspace 650 °C, cryostatic cooling. ⦁⦁ Twister® Stir Bar Sorptive Extraction (SBSE) ⦁⦁ Automated Liner Exchange (ALEX): For ⦁⦁ Dynamic headspace and thermal desorption samples with a heavy matrix load. ⦁⦁ PTV and large-volume injection ⦁⦁ Automated Centrifugation, Solvent ⦁⦁ Automated Liner Exchange (ALEX) Evaporation, Weighing, Filtration, GERSTEL, Inc. ⦁⦁ Multicolumn/multidimensional GC systems Vortexing, and Bar Code Reading 701 Digital Drive, Suite J ⦁⦁ Olfactory detection and preparative fraction collection ⦁⦁ Multidimensional GC with Column Linthicum, MD 21090 ⦁⦁ Pyrolysis Switching: Complex separations using two or more columns. TELEPHONE ⦁ (800) 413-8160 Markets Served ⦁ Olfactory Detection Port (ODP): For ⦁⦁ Food/Beverage, Flavors & Fragrances GC or GC–MS; complete with voice FAX ⦁⦁ Personal Care, Cosmetics, and Over-the-Counter Products recognition, intensity indication, and (410) 247-5887 ⦁⦁ Clinical Research & Forensics peak annotation. E-MAIL ⦁⦁ Electronics, Plastics, Polymers, and Chemicals ⦁⦁ Preparative Fraction Collector (PFC): For [email protected] ⦁⦁ Pharmaceutical, CROs, and Bio-therapeutics GC or GC–MS using up to six traps.

WEB SITE ⦁⦁ Environmental and Industrial Hygiene www.gerstelus.com Facility www.gerstel.com Major Products/Services Full applications support laboratory at US ⦁⦁ MAESTRO Software: Intuitive operation software provides headquarters near Baltimore, Maryland, and YEAR FOUNDED 1967 sample prep by mouse-click and productivity tools for auto- a partner applications laboratory in Chicago, mation. MAESTRO software seamlessly integrates into Agilent Illinois. Our sales and applications team helps ChemStation/MassHunter, or runs in the background on customers use GC and LC analysis techniques LECO, Sciex, Thermo Scientific, and other vendors’ systems. to solve real-world challenges. www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 23 Imtakt USA

⦁⦁ Intrada Organic Acid: HILIC and ion- exchange mixed mode developed as a single-column solution for the analysis of all organic acids. ⦁⦁ Dacapo DX-C18: 2.5 µm particle C18 column designed with a revolutionary dual matrix particle technology, optimized for extreme pH conditions. ⦁⦁ Intrada Amino Acid: The world’s first mixed-mode column designed to sepa- rate amino acids without the need for any derivatization. ⦁⦁ Intrada SEC: Silica based SEC column designed for use in SEC-MS compatible conditions. GPC and GFC. ⦁⦁ SCHERZO SM-C18: C18 multi-mode column with both anion and cation exchange. ⦁⦁ PRESTO FF-C18: Consists of C18 bonded Company Description  to monodispersed 2 μm non-porous Our HPLC columns have been designed and manufactured silica. It is useful for both biopolymer and by Imtakt Corporation with exceptional craftsmanship and synthetic polymer separations. Ideal for decades of industry experience. Truly some of the most unique intact antibody and ADC analysis. column chemistries in the market today, our columns give ⦁⦁ CADENZA CD-C18: High resolution; 25– chromatographers: 50% lower pressure; best with hydropho- ⦁⦁ High resolution bic compounds, molecular recognition, ⦁⦁ 25–50% lower pressure and small molecule isomer separation. ⦁⦁ Novel chemistry. ⦁⦁ Cadenza HS-C18: High resolution C18 col- umn designed to accept direct injection of Chief Chromatographic Techniques Supported biological samples. No need to protein pre- Imtakt USA ⦁⦁ High performance liquid chromatography cipitate your samples for high throughput 2892 NW Upshur St. Suite A ⦁⦁ Ultrahigh-pressure liquid chromatography early drug discovery or clinical lab testing. Portland, OR 97210 ⦁⦁ Liquid chromatography–mass spectrometry ⦁⦁ UNISON UK-AMINO: Revolutionary aque- TELEPHONE ⦁⦁ Reversed-phase ous durability for aminopropyl phase; (215) 665-8902 ⦁⦁ Normal-phase aqueous to non-aqueous normal phase Toll Free: ⦁⦁ Anion exchange separation. (888) 456-HPLC ⦁⦁ Cation exchange FAX ⦁⦁ Multi-mode Facility (501) 646-3497 ⦁⦁ Small-molecule separations We are located in Portland, Oregon, and ⦁ E-MAIL ⦁ Protein separations deliver next day for 95% of North American [email protected] orders, and always within one week. Markets Served WEB SITE All LC and LC–MS markets, including: pharmaceutical, biotech, www.imtaktusa.com contract research, forensic, clinical diagnostic, food and YEAR FOUNDED beverage, industrial, chemical, environmental, and academic 2003 research.

Major Products/Services  A sample of some HPLC column phases from Imtakt includes: 24 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com KIN-TEK Analytical, Inc.

manufacturers, R&D, Universities, and many more!

Major Products/Services FlexStream™ Gas Standard Generator Automated expandable modular permeation system. 491Flex™ Gas Standard Generator Manually operated expandable modular permeation system. EcoFlex™ Gas Standard Generator Manually operated stand-alone permeation module.

Span Pac™ H2O Trace Moisture Generator Manually operated trace moisture permeation system. Span Pac™ I Industrial Gas Standard Generator Industrial permeation system for on-line calibration of process GCs. Company Description Span Check™ Portable Gas Standard KIN-TEK Analytical, Inc. (KIN-TEK), manufactures and sells Generator Portable permeation instrument permeation devices and instrumentation used for creating for field calibration and verification of trace concentration calibration gas standards for simple and monitors and detectors. complex gas mixtures. KIN-TEK was founded in 1970, and CO395 Flex™ Certification Oven developed Trace Source™ Permeation Tube technology used Stand-alone permeation tube oven. in KIN-TEK’s Gas Standard Generators to provide accurate, on- Trace Source™ Disposable and Refillable demand calibration standards (ppmv to pptrv). Calibration gas Permeation Tubes >550 certified standards. standards are used for calibrating gas chromatographs, and over 550 chemical compounds are available, each with a Calibration Recertification/Calibration Services, Certificate traceable to the National Institute of Standards and Installation, and Training Services are offered Technology (NIST). KIN-TEK provides trace calibration gas for KIN-TEK Standard Gas Generators and KIN-TEK Analytical, Inc. solutions to customers worldwide for laboratory, field (portable), Trace Source™ Permeation Tubes. 504 Laurel St and process industries! La Marque, TX 77568 Facility TELEPHONE Chief Chromatographic Techniques Supported Our corporate headquarters is in La Marque, (409) 938-3627 ⦁⦁ Calibration gases Texas. All manufacturing, certifications, and

FAX ⦁⦁ Gas standards operations take place at this facility. (409) 938-3710 ⦁⦁ GC calibration ⦁⦁ GC/MS calibration E-MAIL [email protected] ⦁⦁ Trace gas standards ⦁⦁ Permeation tubes WEB SITE ⦁⦁ Permeation tube supplies www.kin-tek.com

YEAR FOUNDED Markets Served 1970 KIN-TEK delivers trace gas calibration product solutions and services worldwide to solve customer problems in the laboratory, field (por- table), and process industries. Industries served include Analyzer Manufacturers, Aerospace and Aviation, Environmental, Food & Beverage, Petrochemical, Pharmaceutical, Refineries, Semiconductor www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 25 LECO Corporation

and quantify both target and unknown analytes, dramatically reducing overall analysis times and increasing productivity.

Products include: ⦁⦁ Pegasus® BT GC–TOFMS: The tried- and-trusted reliability and durability of our Pegasus brand in a convenient benchtop unit ⦁⦁ Pegasus BT 4D GCxGC–TOFMS: Four dimensions of analytical resolution offer a more complete analysis in a benchtop instrument ⦁⦁ Pegasus GC-HRT+: GCMS with industry- leading resolution (up to 50,000) and mass accuracy for high-information content analysis ⦁⦁ Pegasus GC-HRT+ 4D: Combining the highest performance GCxGC and TOFMS Company Description on the market with High Resolution For over 80 years, industries around the world have trusted LECO Deconvolution®(HRD®) to deliver technologically advanced products and solutions. Today’s technologies for separation science reflect LECO’s commitment to Facility understanding your lab’s challenges and providing solutions that can LECO’s global headquarters in St. Joseph, Michi- investigate highly complex samples while streamlining your analysis. gan include the Elizabeth S. Warren Technical LECO instruments offer unparalleled separation, accuracy, resolving Centre, a 28,000 square-foot facility exclusively power, deconvolution, and speed to increase your lab’s productivity dedicated to the research and development of using GC–TOFMS, GCxGC, GCxGC–TOFMS, and high-resolution GC– innovative instrumentation and equipment for and GCxGC–TOFMS. For more information, please visit them on the LECO’s separation science line. LECO has more LECO Corporation web at www.leco.com. than 25 subsidiaries worldwide, plus additional 3000 Lakeview Avenue international distributors. St. Joseph, MI 49085 Chief Chromatographic Techniques Supported ⦁ TELEPHONE ⦁ GC–TOFMS (800) 292-6141 ⦁⦁ High Resolution GC–TOFMS (269) 985-5496 ⦁⦁ GCxGC–FID/ECD ⦁⦁ GCxGC–TOFMS FAX ⦁⦁ High Resolution GCxGC–TOFMS (269) 982-8987 E-MAIL Markets Served [email protected] LECO Separation Science instruments are used in a variety of WEB SITE applications, helping users to uncover unidentified compounds in www.leco.com their samples. Markets include metabolomics, consumer product

NUMBER OF EMPLOYEES safety, food, flavor, fragrance, environmental, and energy and 717 fuels analysis.

YEAR FOUNDED

1936 Major Products/Services An ideal choice for laboratories investigating highly complex samples, LECO products feature exclusive ChromaTOF® brand software with all the tools needed to easily locate, identify, 26 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com LabVantage Solutions, Inc.

Major Products/Services LabVantage LIMS­—available in Enterprise, Express, and Accelerator editions for specific industries—is entirely configurable, without needing any coding. It features modern technology and architecture that enable compliant user-access from any device; its zero footprint means no client programs or plugins to install and validate; and it can be installed on-premise or via cloud-hosting services. The LIMS platform embeds an optional ELN, LES, and, soon, SDMS. LabVantage customers are supported locally from nearly two dozen global offices with support and service contracts, as well as training.

Company Description LabVantage Solutions is the recognized leader in enterprise laboratory software and service. Its integrated informatics platform includes laboratory information management systems (LIMS), an electronic laboratory notebook (ELN), a laboratory execution system (LES), and, starting in 2020, a scientific data management system (SDMS). These systems support hundreds of concurrent users and seamlessly interface with instrument and other enterprise systems. More than 1500 customer sites across a wide range of industries rely on LabVantage’s highly configurable, purpose- LabVantage Solutions, Inc. built, 100% web browser-based platform to innovate faster in 265 Davidson Avenue, Suite the R&D cycle, improve manufactured product quality, achieve 220, Somerset, NJ, 08873 accurate record-keeping, and comply with global regulatory TELEPHONE requirements. (908) 707-4100

FAX Chief Chromatographic Techniques Supported (732)560-0121 LabVantage supports all analytical techniques, including all

E-MAIL chromatographic techniques, by interfacing to any commercial [email protected] chromatography data system (CDS). WEB SITE Markets Served Labvantage.com LabVantage supports more than 1500 customer sites in the NUMBER OF EMPLOYEES life science, pharmaceutical, medical device, biobank, food Approximately 500 & beverage, consumer packaged goods, oil & gas, genetics/ YEAR FOUNDED diagnostics, and healthcare industries. With nearly two dozen 1983 offices worldwide, LabVantage provides local support to its global customers. www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 27 MACHEREY-NAGEL

is still packed in stainless steel columns by

MN. These columns with various C18 modi- fications are validated in many drug testing methods. In recent years, new highly pure and ultra-spherical HPLC and UHPLC silica gels followed. Today the column range of totally po- rous NUCLEODUR® and fused-core NU- CLEOSHELL® comprise more than 25 station- ary phases–‑including a hydrolytically stable biphenyl phase with enhanced retention for aromatic and unsaturated substances. More information: www.mn-net.com/HPLC MACHEREY-NAGEL, a pioneer in thin- layer chromatography is manufacturing glass plates as well as aluminum and plastic Company Description sheets coated with silica gels and other Since 1911, MACHEREY-NAGEL represents high quality, innova- sorbents for TLC and HPTLC. The modern tion, and reliability in analytical chemistry and life science. As one glass plates SIL HD and Nano-SIL HD are of today’s leading manufacturers, MACHEREY-NAGEL offers a characterized by outstanding dyeability, good broad range of products for chromatography, filtration, rapid tests, wettability, and abrasion resistance due to an water analysis, and bioanalysis. optimized binder system. MN´s expertise in manufacturing of chromatography media More information: www.mn-net.com/TLC is based on many years of experience in silica technology. The Classical CHROMABOND® silica gel development of the spherical HPLC silica NUCLEOSIL® and the phases with various modifications (for ex-

introduction of commercially available ready-to-use TLC plates ample, C18, C8, NH2, OH, CN) and modern and sheets are just two milestones emphasizing the pioneering polymeric resins are widely used for sample tasks of MN in promoting these separation techniques. preparation in environmental analysis, food safety control, or clinical applications. The Chief Chromatographic Techniques Supported hydrophilic-lipophilic balanced polymeric MACHEREY-NAGEL ⦁⦁ SPE and Flash Chromatography SPE adsorbent CHROMABOND® HLB and GmbH & Co. KG ⦁⦁ HPLC the CHROMABOND® HR-Xpert line of poly- Neumann-Neander-Str. 6-8, ⦁⦁ TLC styrene-divinyl based reversed-phase and 52355 Düren, Germany ⦁⦁ GC mixed-mode ion-exchange resins offers a full [email protected] ⦁⦁ Chromatographic accessories (such as syringe filters, vials, toolbox of innovative SPE products. MACHEREY-NAGEL. and caps) More information: www.mn-net.com/SPE 2850 Emrick Blvd., Bethlehem, PA 18020, USA Markets Served Facility [email protected] MN serves laboratories all over the world with HPLC, GC, and The headquarters in Germany and SPE columns, TLC plates and sheets, and syringe filters, as well as branches in the United States, Switzerland, WEB SITE www.mn-net.com suitable vials and caps. Customers from many different industries and France, as well as a globally operating (such as the food and beverage industry, pharmaceutical industry network of distributors in more than 150 NUMBER OF EMPLOYEES and healthcare, chemical industry, institutes for environmental countries, ensures worldwide availability ca. 650 analysis, biotechnology, universities and research institutes) of MN products and services. YEAR FOUNDED benefit from optimal and reliable solutions for analytical tasks in 1911 method development and routine analysis.

Major Products/Services  NUCLEOSIL®, one of the first spherical silica gels in the 1970s, 28 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Markes International

Major Products/Services Markes is globally recognized for its innovation, high-quality products, unrivalled technical expertise, and high level of customer service within the field of analytical TD. As a global technology leader of thermal desorption for GC, Markes has introduced many highly successful products to the analytical laboratory: ⦁⦁ Centri multi-technique sample precon- centration and injection platform, de- livering enhanced analytical sensitivity and higher throughput ⦁⦁ UNITY-xr universal thermal desorption unit for sorbent sampling tubes ⦁⦁ TD100-xr fully automated TD system for sorbent tubes ⦁⦁ ULTRA-xr TD autosampler ⦁⦁ CIA Advantage-xr system for automated Company Description cryogen-free analysis of air and gas Markes International is a multi-award-winning specialist sampled using canisters manufacturer of instrumentation for trace organic analysis. ⦁⦁ TT24-7 for continuous on-line monitoring Markes has a well-deserved reputation for its application ⦁⦁ Micro-Chamber/Thermal Extractor for fast expertise in the field of sample preparation (thermal sampling of emissions from products and desorption, SPME, headspace and high-capacity sorptive materials extraction) and supplying a comprehensive range of ⦁⦁ Wide range of accessories and supplies instruments, sampling equipment, and supplies that enhance for sample preparation Markes International Ltd. the analytical capability of GC–MS. Gwaun Elai Medi-Science Campus, Llantrisant, RCT, Facility CF72 8XL, UK Chief Chromatographic Techniques Supported Markes International’s factory, technical Markes International GmbH ⦁⦁ Thermal desorption (TD) as a sample concentration and center, and headquarters are located in Bieberer Straße 1–7, 63065 introduction technique for GC South Wales, UK. The company also has Offenbach am Main, Germany ⦁⦁ Automated sample preparation and concentration technical centers in Germany, the United Markes International, Inc. (headspace, headspace–trap, SPME, SPME–trap, and high- States, and China. It also supports a global 2355 Gold Meadow Way, capacity sorbent extraction) distributor network. Markes International Gold River, CA 95670, USA is part of the Schauenburg Analytics Ltd Markes Instruments (Shanghai) Markets Served group of companies, which includes its Co., Ltd, Unit 1002, Building 1, ⦁⦁ Environmental sister company SepSolve. No. 418 Guilin Road, Shanghai, ⦁⦁ Food and drink 200233, P. R. China ⦁⦁ Forensic TELEPHONE ⦁⦁ Defense and Homeland Security +44 (0)1443 230935 (UK) ⦁⦁ Consumer and industrial products +49 (0)69 6681089-10 (Germany) ⦁⦁ Automotive +1 866-483-5684 (toll-free) (USA) ⦁⦁ Human health +86 21 5465 1216 (China) ⦁⦁ Chemical ecology WEB SITE Markes’s global customer base includes major industry, www.markes.com government agencies, academia, and the contract service NUMBER OF EMPLOYEES: 160 laboratory sector. YEAR FOUNDED: 1997 www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 29 Merlin Instrument Company

Major Products/Services The Merlin Microseal is a microvalve alternative to the conventional silicone rubber septa used in gas chromatography (GC). Its unique design gives it high pressure capability and resistance to wear, which results in long life and excellent chromatographic performance. The Microseal is available for all major GC manufacturers’ instruments. The Merlin MicroShot injector enables convenient and repeatable manual injec- tions for Gas Chromatography (GC). Each injector is calibrated to a set volume and injection is made by a simple fast motion.

Company Description Merlin Instrument Company designs and manufactures innovative, high-quality accessories for gas chromatography and other analytical techniques. Our products are compatible with all major GC instruments, and are widely distributed by GC manufacturers and chromatography supplies companies. We offer the Merlin Microseal, a long-life alternative to Merlin Instrument conventional GC septa, and the Merlin Microshot, a simple Company tool for precise and repeatable manual GC injections. We also 7000 S. Broadway partner with instrumentation companies and users to develop STE 1As custom sampling and sealing solutions for a wide range of Centennial, CO 80122 applications. TELEPHONE (303) 794-4135

E-MAIL [email protected] Chief Chromatographic Techniques Supported ⦁⦁ GC WEB SITE ⦁ www.merlinic.com ⦁ MS

YEAR FOUNDED 1987 30 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com MicroSolv Technology Corporation

Facilities Production facility, technical support laboratory, and headquarters are in the greater Wilmington, North Carolina area.

Company Description A privately held firm in the greater Wilmington, NC area that acquires, develops, and manufactures new technologies and products for separation sciences. They serve pharma, chemistry, medical and clinical R&D, environmental, forensic, and biological laboratories worldwide through their distribution network.

Chief Chromatographic Techniques Supported MicroSolv Technology ⦁⦁ HPLC Corporation ⦁⦁ LC–MS 9158 Industrial Blvd. NE ⦁⦁ Capillary electrophoresis Leland, NC 28451

TELEPHONE Market Served (732) 380-8900 All analytical and biology laboratories that use HPLC, LC–MS, GC, GC–MS, SFC, or CE in all markets segments and in all FAX countries around the world. (910) 769-9435

E-MAIL [email protected] Major Products/Services WEB SITE Silica hydride based HPLC columns under the www.mtc-usa.com Cogent TYPE-C™ Silica brand name, the RSA™ brand

NUMBER OF EMPLOYEES autosampler vials (Reduced Surface Activity), AQ™ Under 25 Advanced Quality Syringe Filters, and the CElixir™ brand of dynamic coatings for CE. An online learnables YEAR FOUNDED section serves chromatographers and provides free 1992 access to chromatographic methods and tips for making chromatography great and for problem solving. www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 31 Neta Scientific, Inc.

Major Products/Services We enable our customers’ mission by centralizing access to superior laboratory supplies and supply chain management. Our unique ability to provide pass-through support for major brand suppliers, as well as OEM contracts, parts, consumables, and services, give you back the time you need.

Facility The corporate office and main warehouse are based in Hainesport, New Jersey. We also maintain additional representation throughout the United States.

Company Description For 20 years, Neta Scientific, Inc. has been a leading distributor of laboratory supplies and solutions. Our mission is to offer our customers access to a robust catalog of world-leading brands to more effectively conduct cutting- edge research and development. We are an MBE and WBE certified business headquartered in Hainesport, NJ, with representation throughout the United States.

Chief Chromatographic Techniques Supported ⦁⦁ Chromatography Neta Scientific, Inc. ⦁⦁ Life Sciences 4206 Sylon Blvds ⦁⦁ Labware Hainesport, NJ 08036 ⦁⦁ Chemicals & Reagents TELEPHONE ⦁⦁ Lab Equipment (800) 343-6015 ⦁⦁ Safety & Cleaning FAX (609) 265-8213 Markets Served

E-MAIL Neta Scientific serves research and development labs [email protected] throughout North America, encompassing the pharmaceutical, biotech, life sciences, academia and research institutions, WEB SITE government facilities, food and flavors, industrial testing, and www.netascientific.com chemical industries. NUMBER OF EMPLOYEES 40+

YEAR FOUNDED 1999 32 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Optimize Technologies, Inc.

Major Products/Services ⦁⦁ EXP (UHPLC) fittings, filters, nano- technologies, trap columns, analytical 34 YEARS OF INNOVATION columns, and guard columns rated to 20,000+ psi (1400+ bar) ⦁⦁ OPTI-MAX Cartridge Check Valve systems for multiple brand/model pumps ⦁⦁ Replacement seals and pistons for multiple brand/model pumps ⦁⦁ Guard columns in various stationary phases and custom packed

® ⦁⦁ Filtration products for reservoirs, pre-column, and in-line applications ⦁⦁ Autosampler and injection valve replacement parts ⦁⦁ Trapping columns in various sizes and formats for LC–MS applications ⦁⦁ Quick connect hardware for fittings, columns, and pre-column applications ⦁⦁ Custom design services and manufacturing for specific instruments and applications

Company Description Facility In 1985, Optimize Technologies was founded on the simple idea Optimize products are designed and of offering inventive products for HPLC at the highest quality. manufactured at our facility in Oregon City, Since then, the company has grown from simple beginnings Oregon, in our state-of-the-art CNC shop, to the present worldwide recognized brand. Although the and tested in our on-site laboratory. In company has grown, the foundation remains the same: Optimize addition to highly qualified machinists, we Technologies is dedicated to providing the highest quality HPLC, have engineers and chemists collaborating UHPLC, and LC–MS products available. We combine innovative under one roof to design and manufacture Comp Info 1 Name design and superior performance to create products that are products specifically suited for the scientific Optimize Technologies,Address Inc. straightforward lab solutions. We promise to bring you innovative industry. 13993Address Fir Street, products that offer unmatched performance and quality—all OregonCity, City, ST OR ZIP 97045 backed with responsive and effective customer service.

TELEPHONE (503)(xxx) 557-9994xxx-xxxx Chief Chromatographic Techniques Supported ⦁⦁ HPLC FAX ⦁⦁ UHPLC (503)(xxx) 557-9995xxx-xxxx ⦁⦁ Fluid transfer components E-MAIL ⦁⦁ LC–MS [email protected] [email protected] ⦁⦁ 2D LC–MS WEB SITE www.optimizetech.comwww.xxxxxxx Markets Served ⦁⦁ Chromatography ⦁⦁ Nuclear pharmacy NUMBERYEAR OF FOUNDED EMPLOYEES 1985xxx ⦁⦁ Pharmaceuticals ⦁⦁ Clinical ⦁⦁ Drug discovery ⦁⦁ Environmental YEAR FOUNDED ⦁⦁ Mass spectrometry ⦁⦁ Petrochemical xxx ⦁⦁ Proteomics ⦁⦁ Bio-defense www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 33

Markets Served Parker Hannifin ⦁⦁ Agriculture ⦁⦁ Biotechnology ⦁⦁ Chemicals ⦁⦁ Chemical and explosives detection ⦁⦁ Energy ⦁⦁ Environmental ⦁⦁ Inorganic chemicals ⦁⦁ Instrument development ⦁⦁ Life science ⦁⦁ Organic chemicals ⦁⦁ Paints and coatings ⦁⦁ Petrochemicals ⦁⦁ Pharmaceuticals ⦁⦁ Plastics

Major Products/Services Our gas generators serve: ⦁⦁ Gas chromatographs ⦁⦁ LC–MS ⦁⦁ FT-IR spectrometers ⦁⦁ ICP emission spectrometers ⦁⦁ TOC analyzers ⦁⦁ Atomic absorption spectrophotometers ⦁⦁ Nuclear magnetic resonance (NMR) ⦁⦁ Rheometers/thermal analyzers ⦁⦁ Sample evaporators/concentrators ⦁⦁ Mass spectrometers Company Description Parker Hannifin is a Fortune 250 global leader in motion Facilities and control technologies. For more than 100 years, Parker’s Parker designs, tests, and manufactures engineering expertise and broad range of core technologies all gas generators in Haverhill, MA, USA. have helped solve the world’s greatest engineering challenges. Our ISO 9001 facility employs the latest Parker is a global leader in innovation, industry knowledge, and manufacturing standards to ensure our customer care, with world-class manufacturing that provides for products meet the highest quality, safety, Parker Hannifin superior quality, safety, and sustainability across all our products. and environmental impact standards. Corporation Industrial Gas Filtration Parker’s AGS group developed the first commercial analytical and Generation Division gas generator for laboratory use. Here, we have a passion 242 Neck Road for building high-performance gas generators that provide a Haverhill, MA 01835 quick payback, improve safety, and allow labs to take control TELEPHONE of their supplies. (800) 343-4048

FAX Chief Chromatographic Techniques Supported (978) 556-7501 ⦁⦁ GC–FID, FPD, NPD ⦁⦁ GC–HALL, ECD, TCD, HST-Wire E-MAIL ⦁⦁ Capillary separations [email protected] ⦁⦁ Packed separations WEB SITE labgasgenerators.com

NUMBER OF EMPLOYEES 55,000

YEAR FOUNDED 1924 34 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Peak Scientific

Company Description With over two decades in pioneering reliable gas generator technology, Peak Scientific develops market-leading nitrogen, hydrogen, and zero air systems mainly for the fields of LC–MS and GC. An on-site gas generator from Peak Scientific is the practical and cost-effective alternative to pressurized cylinders. Peak Scientific Our forte is in providing our customers with unrivaled Fountain Crescent peace of mind and hassle free gas generation solutions Inchinnan Business Park for their specific laboratory demands, backed up by our Inchinnan world-class technical support and ongoing service care PA4 9RE throughout the generator’s lifespan. With a rapid response Scotland, UK and offices on every continent, we deliver a local service on a global scale.

TELEPHONE +1-866-647-1649 Chief Chromatographic Techniques Supported ⦁⦁ LC–MS FAX +1-978-608-9503 ⦁⦁ GC E-MAIL Markets Served [email protected] ⦁⦁ Laboratory WEB SITE www.peakscientific.com Major Products/Services NUMBER OF EMPLOYEES ⦁⦁ Nitrogen Generators USA: 50+ ⦁⦁ Hydrogen Generators Elsewhere: 450+ ⦁⦁ Zero Air Generators

YEAR FOUNDED 1997 www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 35 PharmaFluidics

for applications in metabolomics, proteomics and lipidomics, but also mAb and ADC characterization, as well as host cell proteins detection and quantification. For fast and easy clean-up and enrichment of peptide samples prior to injection, PharmaFluidics also has a trapping column compatible with the reversed-phase analytical C18 nano-LC columns.

Facility PharmaFluidics’ headquarters are located at the Tech Lane Ghent Science Park (Campus A) in Ghent (Belgium), a vibrant ecosystem of international corporate R&D centers, public research institutes, and high-tech growth enterprises. International commercialization of the PharmaFluidics’ Company Description products is organized by either a direct Introducing micro-Chip manufacturing technology in sales team in Europe or by indirect sales analytical chromatography, PharmaFluidics uses a lithographic channels the US and the rest of the world. etching process to create a perfectly ordered separation bed on a silicon chip. PharmaFluidics wants to bring liquid chromatography to a next level of efficiency in all aspects, by providing separation devices with an unprecedented reproducibility and robustness, in a format that enables extreme user-friendliness and resolution, and opens the way to further miniaturization and integration.

Chief Chromatographic Techniques Supported ⦁⦁ Nano LC–MS ⦁⦁ Capillary LC–MS

PharmaFluidics Markets Served Technologiepark-Zwijnaarde 82 The µPAC™ micro-Chip based chromatography columns B9052 Ghent (Zwijnaarde) are commercially available for proteomic, lipidomic, and Belgium metabolomic profiling, with specific applications in bio- E-MAIL marker, diagnostics and drug research & development. [email protected] µPAC™ also enables a very detailed characterization of WEB SITE biopharmaceuticals and biosimilars, detecting amino acid www.pharmafluidics.com substitutions and post-translational modifications.

Major Products/Services To serve your custom separation needs, µPAC™ nano LC columns (>2.5 µL/min) (50–200 cm) and µPAC™ capLC column (1–15 µL/min) are equipped with connectors matching your application. Stationary phase chemistry is currently focusing on reversed phase HPLC C18 separations 36 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Photonis Scientific, Inc.

Markets Served Mass spectrometry, nuclear detection, medical instrumentation, pharmaceutical safety, industrial instrumentation, and image intensification, as well as the custom design and manufacture of detectors, sensors, and ion mobility analyzers.

Major Products/Services Photonis offers a complete range of high performance scientific and medical detector products. Our market includes electron multipliers, microchannel plates, mass spectrometry fiber optics and resistive glass, advance performance time-of-flight detectors, image intensifiers, ion mobility analyzers, neutron imagers, and other related products. Our detection products are found in most of today’s technology-based markets, including analytical instrumentation, medical diagnostics, chemistry, drug discovery, high-energy physics, space exploration, and scientific research. Photonis is the largest supplier of standard, retrofit, and custom Company Description detectors in the mass spectrometry, residual Photonis is a multinational high-technology group, with more than gas analyzer, and electron microscope 40 years of experience in manufacturing, sales, and innovation, markets, providing advanced detector designs specializing in charged particle and photon sensor technology. for the highest sensitivity through superior The group operates internationally in the night vision, industrial, signal collection and noise reduction. Photonis Scientific 660 Main Street scientific, and medical markets. Sturbridge Business Park Facilities Sturbridge, MA 01566 Chief Chromatographic Techniques Supported Sturbridge, MA; Lancaster, PA; Brive, ⦁⦁ Liquid chromatography detectors France; Roden, Netherlands TELEPHONE ⦁⦁ Ion mobility spectrometry (508) 347-4000 ⦁⦁ Mass spectrometers FAX ⦁⦁ UV–vis fixed wavelength (508) 347-3849 ⦁⦁ UV–vis variable wavelength E-MAIL ⦁⦁ Gas chromatography detectors and accessories [email protected] ⦁⦁ Detector accessories ⦁⦁ Mass spectrometers: high-resolution WEB SITE ⦁⦁ Mass spectrometers: low-resolution www.photonis.com ⦁⦁ Mass spectrometers, residual gas analysis, magnetic NUMBER OF EMPLOYEES ⦁⦁ Mass spectrometers, residual gas analysis, quadrupole US: 150 ⦁⦁ ICP-MS Elsewhere: 900 ⦁⦁ Time-of-flight mass spectrometry

YEAR FOUNDED ⦁⦁ Other detectors and accessories 1937 ⦁⦁ Supercritical fluid chromatography detectors and accessories ⦁⦁ Radioactivity ⦁⦁ Neutron imaging www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 37 Pickering Laboratories, Inc.

Artificial Perspiration ⦁⦁ Eccrine and Apocrine human mimics ⦁⦁ Corrosion testing ISO 3160 ⦁⦁ Hardware and finishes ANSI-BHMA A 156.18 ⦁⦁ Colorfastness AATCC Test Method 15, ISO 105-B07, ISO 105-E04 ⦁⦁ Automotive materials testing GMW 14334 ⦁⦁ Other official testing procedures (DIN, ISO, BS, EN, AATCC) Artificial Saliva ⦁⦁ Fusayama/Mayer formulation ⦁⦁ Colorfastness DIN 53160 ⦁⦁ Biodegradability AFNOR NF S91-141 ⦁⦁ Drug dissolution testing ⦁⦁ Dental and medical research Artificial Cerumen (earwax) Artificial Sebum Company Description Artificial Urine Formulations Pickering Laboratories, Inc. manufactures products for both analytical laboratories and consumer product testing laboratories. Markets Served Pickering Laboratories designs, Application-Specific Analytical Products manufactures, and sells specialized analytical Our analytical columns, high-purity reagents, and post-column instrumentation and chemistry for the derivatization instruments pair with any manufacturer’s HPLC environmental, pharmaceutical, food testing, to enable specialized application analysis. Some of our more clinical, and cosmetics industries, as well as popular applications include: Amino Acids, Carbamates, biochemical laboratory markets. Pickering Glyphosate, Aminoglycoside Antibiotics, Polyether Antibiotics, Additionally, Pickering Laboratories Laboratories, Inc. Biogenic Amines, Aflatoxins, and Paralytic Shellfish Toxins. provides testing solutions for consumer 1280 Space Park Way Analytical reagents and eluents are prepared according to product testing facilities, the textile industry, Mountain View, CA 94043 official AOAC, EPA, and FDA methods. and electronics manufacturers around the

TELEPHONE Pickering’s post-column derivatization instruments connect world. (800) 654-3330 to virtually any HPLC, and can be easily configured to detect a An extensive network of distributors (650) 694-6700 wide range of compounds with high sensitivity and specificity. represents Pickering Laboratories products worldwide, and can be found on our webpage. FAX (650) 968-0749 Supported Applications ⦁⦁ Glyphosate herbicide Facility E-MAIL ⦁⦁ Carbamate pesticides Our Mountain View, California, headquar- [email protected] ⦁⦁ Biogenic amines ters includes research and development, WEB SITE ⦁⦁ Amino Acids analysis manufacturing, technical support, training, www.pickeringlabs.com ⦁⦁ Polyether antibiotics administration, and customer service. www.pickeringtestsolutions.com ⦁⦁ Mycotoxins Products are manufactured in

NUMBER OF EMPLOYEES ⦁⦁ Cannabinoids accordance with specified quality-control 18 guidance, and a certificate of analysis is Consumer Product Testing Solutions provided for all chemistry products. YEAR FOUNDED 1980 Pickering Laboratories’ Product Testing Solutions are used for product development, quality testing, and research applications. Custom formulations available. 38 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Postnova Analytics Inc.

Facilities NORTH AMERICA Postnova Analytics Inc. 230 South, 500 East, Suite 110 Salt Lake City, UT 84102, USA Tel: +1 801 521-2004 Fax: +1 801 521-2884 [email protected]

EMEAI Postnova Analytics GmbH Max-Planck-Strasse 14 86899 Landsberg, Germany Tel: +49 8191 985 688-0 Fax: +49 8191 985 688-99 [email protected]

UNITED KINGDOM Postnova Analytics UK Ltd. Company Description Unit 64, Malvern Hills Science Park, Postnova Analytics offers the worldwide unique FFF-Platform, Malvern, Worcestershire which is a range of different field-flow fractionation (FFF) WR14 3SZ, UK and light scattering systems (MALS) for advanced separation, Tel: +44 1684 585167 fractionation, and characterization of nanoparticles, proteins, [email protected] polymers, and bio-macromolecules. Postnova offers a truly complete FFF-MALS product range, which is widely used for applications in biopharmaceutical, nanotechnology, environmental, food, cosmetics, and polymer science. The Postnova FFF-Platform can also be ideally coupled to dynamic light scattering (Malvern Zetasizer Nano DLS) and inductively coupled plasma–mass spectrometry (Agilent Technologies ICP-MS), which provides high resolution size and molar mass Postnova Analytics Inc. 230 South, 500 East, Suite 110 separations as well as characterization and element speciation. Salt Lake City, UT 84102 Separation Techniques Provided TELEPHONE ⦁⦁ Field-flow fractionation (FFF) +1 (801) 521-2004 ⦁⦁ Asymmetrical Flow FFF (AF4) FAX ⦁⦁ Electrical Asymmetrical Flow FFF (EAF4) +1 (801) 521-2884 ⦁⦁ Thermal FFF E-MAIL ⦁⦁ Centrifugal FFF [email protected] ⦁⦁ Multi-angle light scattering (MALS)

WEB SITE www.postnova.com www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 39 Proton OnSite

Major Products/Services Proton OnSite offers hydrogen, nitrogen, and zero air gas generators to fit the needs of any laboratory. These units are suited to provide ultra-high purity gas with consistent and predictable results. Also, Proton OnSite is the only provider of “Lab Server” solutions, allowing large labs to supply numerous mass spec instruments from a single, central generator. Our line of on-site gas generators is a simpler, less expensive, and less complex approach to supplying superior gas for laboratory and scientific applications. These generators are compact in size, and available in stackable configurations to maximize your available laboratory space.

Company Description Proton OnSite, a wholly owned subsidiary of Nel ASA, Oslo, Norway (OSE:NEL), manufactures hydrogen, nitrogen, and zero air generators that you can rely on. Our advanced proton exchange membrane (PEM) electrolysis systems, coupled with uncompromising attention to excellence and quality, enable us to deliver, install, and support gas generation on every continent. No other company has as large of a commercial fleet, or as long of a history with reliability, as Proton OnSite. Exclusively focused on customer solutions, we have the experience and products to ensure success. Proton OnSite 10 Technology Dr. Markets Served Wallingford, CT 06492 ⦁⦁ Cannabis sciences TELEPHONE ⦁⦁ Environmental sciences (203) 678-2300 ⦁⦁ Food and beverage industries FAX ⦁⦁ Forensic sciences (203) 949-8016 ⦁⦁ Life sciences

E-MAIL ⦁⦁ Petrochemical industry [email protected] ⦁⦁ Pharmaceutical industry

WEB SITE www.ProtonOnsite.com

NUMBER OF EMPLOYEES 100

YEAR FOUNDED 1996 40 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com

Markets Served Polymer Standards Service Fully featured PSS applications provide solutions for the following markets: ⦁⦁ Biopharma USA Inc. PSSUSA, Inc. ⦁⦁ Chemical manufacturing ⦁⦁ Food analysis ⦁⦁ Medical analysis ⦁⦁ Pharmaceutical analysis

Major Products/Services Reference Materials ⦁⦁ GPC/SEC standards and kits ⦁⦁ Easy-to-use ReadyCal calibration kits ⦁⦁ MALDI kits ⦁⦁ Viscosity and light scattering validation kits ⦁⦁ Certified reference materials ⦁⦁ Deuterated polymers ⦁⦁ Tailor-made polymers High Resolution Columns ⦁⦁ GPC/SEC columns for all organic eluents and all aqueous eluents ⦁⦁ For high and low molecular weight synthetic and biopolymers ⦁⦁ For micro GPC/SEC up to preparative scale ⦁⦁ HighSpeed columns for fast analysis Instrumentation and Software Company Description ⦁⦁ GPC/SEC systems and components PSSUSA is fully dedicated to the advancement of macromolecular ⦁⦁ Light scattering detectors SEC/GPC/LC analytical techniques by developing easy-to-use and ⦁⦁ Viscosity detectors practical solutions for QC and R&D applications, based on our ⦁⦁ dn/dc instrumentation extensive range of purposefully designed products for macromo- ⦁⦁ WinGPC UniChrom software lecular characterization and continuous research into developing ⦁⦁ PoroCheck software for inverse GPC/SEC instrumentation and software to meet existing and future char- Contract analysis (including HT-GPC acterization requirements. These range from a single molar mass and specialty solvents) reference material, an unmatched range of GPC/SEC columns, ⦁⦁ Contract analysis contract analysis and consulting, to turn-key systems for GPC/SEC ⦁⦁ Molar mass determination multi-detection with light scattering, viscometry, mass spectrom- ⦁⦁ Branching/structure information etry, or fully CFR compliant GPC/SEC systems and the developing ⦁⦁ Method development and transfer fields of 2-D, IPC, TGIC, GPEC by determining molecular weight, ⦁⦁ Complete product deformulation PSS Polymer Standards Service USA Inc. PSSUSA, Inc. size, architecture and composition for synthetic polymers, bio- ⦁⦁ Consulting 160 Old Farm Road, polymers, polysaccharides, and proteins. GPC/SEC Academy and Support Amherst, MA 01002 ⦁⦁ Full services from installation to valida- Chromatographic Techniques Supported tion, operation, and repair TELEPHONE ⦁⦁ Size-exclusion chromatography (SEC) ⦁⦁ GPC/SEC and software training schools (413) 835-0265 ⦁⦁ Gel permeation chromatography (GPC) ⦁⦁ GPC/SEC training in-house tailored to E-MAIL ⦁⦁ Gel filtration chromatography (GFC) customers’ requirements [email protected] ⦁⦁ High-Temperature GPC (HT-GPC) ⦁⦁ NetCommunity with application and pub- WEB SITE ⦁⦁ GPC/SEC viscometry / light scattering / triple lication downloads www.pss-polymer.com ⦁⦁ GPC/SEC mass spectrometry

YEAR FOUNDED ⦁⦁ Two-dimensional chromatography Facility 1994 ⦁⦁ Interaction polymer chromatography (IPC) PSSUSA is located in Amherst, MA. Headquarters of PSS Polymer Standards Service GmbH, are located in Mainz, Germany. PSS also has offices in The Netherlands, and works with a global network of distributor partners. www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 41

⦁⦁ Industrial hygiene Restek Corporation ⦁⦁ Petrochemical ⦁⦁ Pharmaceutical

Major Products/Services Plus 1 Service in everything we do. Living this corporate core value every day ensures we will surpass your expectations every time you contact us! Our customer service team will suggest time- and money-saving options, and is dedicated to getting your products to you fast. Our technical service chemists can help you from set-up to method development. Visit our website, where you can blog with our chemists and review an extensive library of technical publications, chromatograms, product documentation, step-by-step guides, interactive calculators, animations, and educational material. Restek’s commitment to continuous innovation in chromatography sets us apart from our competitors. We introduce and stock hundreds of new products every year, designed by chromatographers for chromatographers. Company Description ⦁⦁ Exceptional columns for UHPLC, HPLC, For over 30 years, Restek has been a leader in developing LC–MS, GC, GC–MS, and GC×GC technologies and manufacturing products for gas and liquid ⦁⦁ Innovative accessories, instrument chromatography (GC and LC), including columns, reference replacement parts, and consumables standards, sample preparation materials, accessories, and ⦁⦁ Air monitoring canisters and sampling more. We have decades of hands-on, practical experience supplies in chemistry, chromatography, and engineering, and our ⦁⦁ Sample prep products reputation for going the extra mile with Plus 1 customer ⦁⦁ Reference standards: stock and service and top-performing products is well known custom-prepared formulations throughout the chromatography community. Restek is proud ⦁⦁ Thousands of innovative products, to assist analysts around the world with monitoring the hundreds of chromatograms quality and safety of air, water, soil, food, pharmaceuticals, Restek Corporation and petroleum. We proactively offer integrated solutions— Facilities 110 Benner Circle products, applications, and assistance—perfectly matched to Restek opened for business in 1985 in Bellefonte, PA 16823 your needs, regardless of your industry. www.restek.com a small business incubator in central TELEPHONE Pennsylvania. Today, more than 500 (800) 356-1688 Chief Chromatographic Techniques Supported employee-owners work, play, and FAX ⦁⦁ UHPLC celebrate milestones in a state-of-the-art (814) 353-1309 ⦁⦁ HPLC 140,000-square-foot facility in Pennsylvania, ⦁⦁ LC–MS in our research facility in California, and in E-MAIL [email protected] ⦁⦁ GC our subsidiary locations in China, England, ⦁⦁ GC–MS France, Germany, Italy, and Japan. WEB SITE ⦁ www.restek.com ⦁ GC×GC NUMBER OF EMPLOYEES Markets Served 500 ⦁⦁ Air monitoring YEAR FOUNDED ⦁⦁ Chemical 1985 ⦁⦁ Clinical ⦁⦁ Environmental ⦁⦁ Food safety ⦁⦁ Forensic 42 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com SepSolve Analytical Ltd

Major Products/Services The wide range of products offered by SepSolve include sample preparation equipment (liquid injection, headspace, SPME, and large-volume injection), robotic autosamplers, thermal desorption instruments, GC×GC technology (including the INSIGHT® flow modulator), the BenchTOF™ range of time-of-flight mass spectrometers with groundbreaking Tandem Ionisation® (simultaneous hard- and soft-ionisation technology), and the powerful ChromSpace® software package.

Facility SepSolve Analytical is part of the Schauenburg Analytics Ltd group of companies, which includes its sister company Markes International. It is Company Description headquartered in Peterborough, UK, SepSolve Analytical is a specialist in providing product and and has offices and a demonstration application packages for GC analysts, which extract maximum laboratory in Canada, which services the analytical data and deliver faster throughput for both research whole of North America. SepSolve also and routine GC applications. Addressing the whole workflow works closely with Markes International process, from sampling to detection, SepSolve provides and Schauenburg Analytical Solutions instrumentation, accessories, and know-how for every step (Shanghai) to support customers of the process, and packages are tailored to meet individual worldwide. laboratories’ needs. SepSolve advises global clients on the most SepSolve Analytical Ltd difficult challenges in analytical science–from environmental 4 Swan Court, Cygnet Park, monitoring to petrochemical analysis and food aroma profiling. Peterborough, PE7 8GX, UK Chief Chromatographic Techniques Supported 826 King Street North, ⦁ Waterloo, Ontario, N2J 4G8, ⦁ GC and GC×GC (and associated software) Canada ⦁⦁ TOF MS ⦁⦁ FID TELEPHONE UK: ⦁⦁ Sample preparation and concentration—thermal desorption, +44 (0)1733 669222 SPME and SPME—trap, high-capacity sorptive extraction, TELEPHONE CANADA: headspace and headspace–trap, and large-volume injection +1 519 206 0055

E-MAIL Markets Served [email protected] ⦁⦁ Food and drink ⦁⦁ Petrochemical WEB SITE www.sepsolve.com ⦁⦁ Fragrance ⦁⦁ Environmental NUMBER OF EMPLOYEES ⦁⦁ Biological monitoring 10 ⦁⦁ Tobacco and e-cigarettes YEAR FOUNDED ⦁⦁ Cannabis 2016 www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 43 Shimadzu Scientific Instruments

Major Products/Services Shimadzu offers a wide range of advanced UHPLC/HPLC, GC, and mass spectrometry sys- tems and components. Key systems include: ⦁⦁ Nexera 40 series UHPLC—Incorporating artificial intelligence, utilizing the Internet of Things, and featuring the most advanced performance features available, the 40 series enables smart, efficient workflows and delivers excellent data quality. ⦁⦁ i-Series Plus Integrated HPLC/UHPLC— Providing the ultimate balance of high- level performance and ease-of-use in an integrated HPLC. ⦁⦁ Nexera Prep SFC—Allows users to over- haul their workflow, reduce labor, and maximize lab resources, while achieving superior fractionation recovery rates for Company Description preparative purification. Shimadzu Scientific Instruments (SSI) is the North American ⦁⦁ LCMS-2020/8040/8045/8050/8060— subsidiary of Shimadzu Corp., headquartered in Kyoto, Japan. User-friendly single-quad and triple quad SSI was established in 1975 to provide analytical solutions LC–MS-MS systems deliver outstanding to a wide range of laboratories in the Americas. With a speed, sensitivity, and reliability. vast installed base and preferred vendor status at many ⦁⦁ Q-TOF LCMS-9030—Allows users to ef- institutions, SSI’s instruments are used by top researchers fortlessly conduct critical qualitative and across the globe, customers who can count on the stability, quantitative analysis with genuine confi- experience, and support only Shimadzu offers. dence and ease. Shimadzu Scientific Instruments ⦁⦁ Nexis GC-2030—Offers a modern ap- 7102 Riverwood Drive Chief Chromatographic Techniques Supported proach to a classic chromatographic Columbia, MD 21046 ⦁⦁ Ultrahigh-pressure LC technique, delivering exceptional perfor- ⦁⦁ Ultra-fast LC mance and faster ROI. TELEPHONE ⦁⦁ Analytical/prep HPLC ⦁⦁ System GC—Fully applicated GC systems (800) 477-1227 ⦁ (410) 381-1227 ⦁ Prep SFC based on industry-standard methods. ⦁⦁ Online SFE-SFC ⦁⦁ GCMS-QP2020/SE/TQ8040/TQ8050— FAX ⦁⦁ LC–MS/MS With Smart Technologies, single-quad and (410) 381-1222 ⦁⦁ Q-TOF LC–MS triple-quad GC–MS/MS systems enable E-MAIL ⦁⦁ GC new possibilities in sensitivity, durability, [email protected] ⦁⦁ Comprehensive LC and GC performance, stability, and reliability.

WEB SITE ⦁⦁ GC–MS/MS www.ssi.shimadzu.com Facilities Markets Served Shimadzu’s US headquarters includes a cus- NUMBER OF EMPLOYEES US: 510 Shimadzu’s product line flexibility enables chromatographers in any tomer service and training center, a solution Worldwide: 12,600 environment to select the instrument best suited to their application. center to showcase technologies, and an Shimadzu instruments are found in a wide range of labs, including innovation center for promoting collaborative YEAR FOUNDED Shimadzu Scientific academic research, pharmaceutical, forensic, food and beverages, projects with customers. Shimadzu’s regional Instruments: 1975 petrochemical, life sciences, and environmental. Shimadzu provides facilities, strategically located around the US, Shimadzu Corporation: 1875 free technical support for the life of the instruments, and encourages provide customers with local sales, service, customer alliances to further product development. and technical support. 44 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Shodex/Showa Denko America, Inc.

Facility Showa Denko America, Inc., based in New York, New York, provides technical and sales support for Shodex HPLC columns for customers and distributors in North and South America. Our stock point in New York allows for fast delivery in the United States. Working with our laboratory in Japan, we support customers’ analytical needs.

Company Description Shodex HPLC columns have been manufactured by Showa Denko K.K. in Japan since 1973. Showa Denko K.K. is one of Japan’s largest chemical companies. Our strength in polymer synthesis led to the production of many unique and durable polymer-based columns. The golden-mechanical fish is a symbol of fast and smooth liquid chromatography analysis.

Chief Chromatographic Techniques Supported HPLC column selection, method development, and trouble shooting, as well as providing educational seminars Shodex/Showa Denko America, Inc. Markets Served 420 Lexington Ave. ⦁⦁ North America Suite #2335A ⦁⦁ South America New York, NY 10170 ⦁⦁ HPLC TELEPHONE ⦁⦁ Size exclusion (212) 370-0033

FAX Major Products/Services (212) 370-4566 Shodex carries thousands of different HPLC columns with

E-MAIL various separation modes. We are best known for high quality [email protected] size exclusion and saccharide analysis columns, as well as unique polymer-based reversed and HILIC phase columns. WEB SITE With additional selections of normal phase, ion exchange, ion www.shodexhplc.com chromatography, high temperature, and multimode columns, YEAR FOUNDED we are confident we can provide the most suitable column 1973 for customers’ needs. TM www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 45 SilcoTek® Corporation

⦁⦁ Process ⦁⦁ Analytical ⦁⦁ Bio/Pharma ⦁⦁ Oil & Gas ⦁⦁ Refining ⦁⦁ Petrochemical ⦁⦁ Semiconductor ⦁⦁ Corrosion ⦁⦁ Coking/Fouling ⦁⦁ Automotive ⦁⦁ Aerospace

Major Products/Services Major Products/Services: SilcoTek offers custom silicon coatings to OEMs and labs worldwide. Send in your stainless steel, alloy, glass, or ceramic part and we’ll apply our signature CVD coatings. Our coating options include: Company Description ⦁⦁ SilcoNert®: The most inert coating SilcoTek Corporation is the world’s leading provider of flow available. path deactivations and coatings. Our patented CVD coating ⦁⦁ Dursan®: An inert, high durability technologies improve analytical reliability, increase system coating. Ideal for lab and field analysis. uptime, and boost your bottom line. SilcoTek’s coatings are used ⦁⦁ Silcolloy®: A high purity corrosion in a large, diverse variety of industries and applications where resistant coating. analytical accuracy and superior performance are critical. Whether ⦁⦁ SilcoKlean®: Prevent carbon coking and for industry-leading chemical compatibility, corrosion resistance, fouling in automotive and aerospace. bio-inertness, or hydrophobicity, SilcoTek coatings will expand the ⦁⦁ SilcoGuard®: A low outgassing, high material limits of your instruments and products. purity coating, ideal for research and SilcoTek Corporation semiconductor applications. 225 PennTech Drive, ® Bellefonte, PA 16823 Chief Chromatographic Techniques Supported ⦁⦁ Dursox : A high purity corrosion- ⦁⦁ Sulfur analysis resistant coating. TELEPHONE ⦁⦁ VOC analysis (814) 353-1778 ⦁⦁ Environmental Facility FAX ⦁⦁ Thermal desorption SilcoTek applies coatings to customer- (814) 353-1697 ⦁⦁ Fenceline monitoring supplied products from their newly

E-MAIL ⦁⦁ CEMS renovated 70,000 square foot facility state [email protected] ⦁⦁ Flare subpart JA of the art facility in Bellefonte, Pennsylvania. ⦁⦁ Stack monitoring Want to speak to an expert about your WEB SITE www.SilcoTek.com ⦁⦁ Protein analysis application? ⦁⦁ NOx, SOx testing Email [email protected] NUMBER OF EMPLOYEES ⦁⦁ GC or call +1 (814) 353-1778. 63 ⦁⦁ LC WEB SITE To find a sales representative nearest to you, www.SilcoTek.com Markets Served please visit YEAR FOUNDED SilcoTek coating technologies are utilized in diverse applications and www.silcotek.com/international-reps 2009 industry segments worldwide. Since 1987, customers have relied on SilcoTek® coatings to improve the performance of their products and increase revenue in the following markets and applications: 46 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Thermo Fisher Scientific

Major Products/Services Thermo Fisher’s advanced analytical solu- tions include: ⦁⦁ The Thermo Scientific Orbitrap Exploris 480 mass spectrometer, which combines proven technology, advanced capabilities, and intelligence-driven data acquisition strategies for rigorous, high-throughput protein identification, quantitation and structural characterization of biotherapeutics and translational biomarkers–all within a compact, benchtop solution. ⦁⦁ The Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer is reinvigorating high-end protein research with expanded abilities to characterize and quantify complex biomolecules; the real-time capabilities of the Orbitrap Company Description Eclipse Tribrid mass spectrometer use Thermo Fisher Scientific Inc. is the world leader in serving science, the latest, built-in intelligence-driven with revenues of more than $24 billion and approximately data acquisition strategies to enhance 70,000 employees globally. Our mission is to enable our experimental efficiency and multiplexed customers to make the world healthier, cleaner, and safer. quantitative analysis of proteomes. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, Facility deliver medicines to market, and increase laboratory productivity. Based in Waltham, Massachusetts, Through our premier brands—Thermo Scientific, Applied Thermo Fisher’s global headquarters is Biosystems, Invitrogen, Fisher Scientific, and Unity Lab Services— a 286,000 square-foot facility designed we offer an unmatched combination of innovative technologies, to reflects the company’s commitment purchasing convenience, and comprehensive services. For more to scientific excellence and employee Thermo Fisher Scientific information, please visit www.thermofisher.com. involvement. Workspaces and common 168 Third Avenue areas encourage open communication, Waltham, MA USA 02451 Chief Chromatographic Techniques Supported and state-of-the-art technology connects ⦁⦁ Liquid chromatography the company’s global hub to its centers of TELEPHONE ⦁⦁ High performance liquid chromatography excellence around the world. (781) 622-1000 ⦁⦁ Ultra-high performance liquid chromatography

WEB SITE ⦁⦁ Ion chromatography www.thermofisher.comx ⦁⦁ Gas chromatography ⦁⦁ Chromatography data system software NUMBER OF EMPLOYEES ⦁⦁ Mass spectrometry Approximately 70,000 ⦁ employees worldwide ⦁ Sample preparation YEAR FOUNDED Markets Served 2006 As the global leader in serving science, Thermo Fisher Scientific provides innovative instruments and user-friendly software to industrial, pharmaceutical, educational, food, environmental, and health markets. www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 47 Tosoh Bioscience LLC

Major Products/Services Tosoh Bioscience LLC offers a compre- hensive line of TOYOPEARL, TSKgel, and Ca++Pure-HA bulk resins for process chromatography. A variety of screening tools, including SkillPak™ 1 mL and 5 mL pre-packed columns are available as an additional tool for convenient scale-up. In addition, bulk media volumes of <1 L are offered for process development. Prepacked columns containing TSKgel media are designed for the analysis of proteins, peptides, other biopolymers, low molecular weight compounds, and organic soluble polymers. The packings in the columns are either silica-based or polymer- based material, in particle sizes ranging from 2 μm to 30 μm. The EcoSEC Elite GPC System and the Company Description EcoSEC High Temperature GPC System Tosoh Bioscience LLC is a major supplier of chromatography are dedicated GPC instruments developed products worldwide, particularly to the pharmaceutical, specifically for polymer analysis. These all- biotechnology, and polymer industries. The company is a in-one systems deliver top performance, division of Tosoh Corporation, a global chemical company with reliability, and superior results. headquarters and manufacturing facilities in Japan. Tosoh’s The LenS3™ multi-angle light scattering portfolio of over 500 TSKgel® and TOYOPEARL® products detector allows direct measurement of encompasses all common modes of liquid chromatography. molecular weight and provides best-in- We manufacture analytical U/HPLC columns and provide bulk class sensitivity. chromatographic resins for the purification of biopharmaceutical Tosoh Bioscience LLC drugs in commercial manufacturing processes. Ca++Pure-HA®, Facilities 3604 Horizon Drive a hydroxyapatite resin for biomolecule purification, is a unique Tosoh Bioscience LLC is located in King Suite 100 resin from Tosoh Bioscience LLC in that it is both the ligand and of Prussia, PA. Supply chain offices: King of Prussia, PA 19406 base bead. Tosoh Bioscience LLC offers dedicated systems for Grove City, OH. European operations: TELEPHONE polymer characterization: the EcoSEC Elite® GPC System, EcoSEC® Griesheim, Germany. Asia is served (484) 805-1219 High Temperature GPC System, and LenS3™ MALS detector. by Tosoh Corporaton in Tokyo, Japan, (800) 366-4875 Shanghai, China, and Singapore. Products FAX Chief Chromatographic Techniques Supported manufactured by Tosoh Corporation, Japan. (610) 272-3028 ⦁⦁ Size exclusion

E-MAIL ⦁⦁ Reversed phase, normal phase (hydrophilic interaction) [email protected] ⦁⦁ Ion exchange ⦁⦁ Hydrophobic interaction WEB SITE ⦁ www.tosohbioscience.com ⦁ Affinity ⦁⦁ Mixed-mode NUMBER OF EMPLOYEES 35 Markets Served YEAR FOUNDED Located in King of Prussia, Pennsylvania, Tosoh Bioscience LLC 1987 provides sales and service to pharmaceutical and biotechnology customers in North and South America. 48 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com UCT, Inc.

Markets Served ⦁⦁ Clinical ⦁⦁ Environmental ⦁⦁ Food safety ⦁⦁ Forensic ⦁⦁ Pharmaceutical ⦁⦁ Veterinary

Major Products/Services ⦁⦁ CLEAN SCREEN® SPE columns ⦁⦁ Refine® Ultra Filtration Columns and Well Plates ⦁⦁ CLEAN UP® SPE columns ⦁⦁ STYRE SCREEN® SPE columns ⦁⦁ XtrackT® SPE columns ⦁⦁ ENVIRO-CLEAN® SPE cartridges ⦁⦁ ENVIRO-CLEAN® universal cartridges ⦁⦁ Chlorofiltr® Sorbents ⦁⦁ QuEChERS ⦁⦁ Quick QuEChERS ⦁⦁ SpinFiltr™ ⦁⦁ LipiFiltr® ⦁⦁ SELECTRA® HPLC columns Company Description ⦁⦁ SELECTRASORB bulk sorbents UCT is always at the forefront of sample preparation technology ⦁⦁ SELECTRA-SIL® derivatizing reagents and a leader in chromatography consumables. The company’s ⦁⦁ SELECTRAZYME® & ABALONASE™ wide range of highly reproducible silica and polymeric SPE β-glucuronidase sorbents provides scientists a consistent extraction technique. ⦁⦁ SELECT pH buffer pouches UCT is in a unique position within the sample prep and ⦁⦁ Positive pressure manifolds chromatographic sorbent industry; we are one of the few ⦁⦁ SPeDRY® 32/48 Position Solvent Evaporator UCT, Inc. manufacturers of chromatographic silane materials. This expertise ⦁⦁ Vacuum manifolds 2731 Bartram Road provides us with greater control and extensive knowledge of the Bristol, PA 19007 chemical processes involved in producing high quality bonded Facility TELEPHONE phases. UCT’s commitment to ensuring customer satisfaction UCT is headquartered in Bristol, Pennsylvania, (215) 781-9255 is accomplished by delivering on our promises: top quality, with a research facility in Scranton, Pennsylva- FAX reproducible chromatographic and sample prep products, and nia. Manufacturing and distribution sites are in (215) 785-1226 unmatched technical support. The company’s sample prep Lewistown, Pennsylvania, and Wexford,

E-MAIL product lines include SPE cartridges and well plates, flash Ireland. UCT is represented worldwide by [email protected] chromatography columns, bulk sorbents, QuEChERS tubes, more than 47 partners and distributors. derivatizing reagents, hydrolyzing enzymes, premeasured buffer WEB SITE salts, and extraction manifolds; chromatography products include www.unitedchem.com HPLC columns and GC liners. All products are designed to meet NUMBER OF EMPLOYEES laboratory sample prep and chromatography needs. >100

YEAR FOUNDED Chief Chromatographic Techniques Supported 1986 ⦁⦁ Solid-phase extraction ⦁⦁ QuEChERS ⦁⦁ HPLC www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 49 Valco Instruments Co., Inc. (VICI)

Precision Sampling manufactures analytical quality syringes, sampling probes, as well as standard and custom formed stainless and special alloy tubing. VICI AG International produces some Valco products as well as the Jour line of HPLC fittings and lab safety products. VICI DBS builds our new line of laboratory hydrogen, nitrogen, and zero air gas generators. Our newest products include the C82 line of UHPLC valves, fittings that permit direct connection of 360 µm tubing, the Universal valve actuator, and the C52 platform integrated HPLC injectors and selectors.

Facility With over 150 state-of-the-art CNC machines, our instruments and components are manufactured to exacting Company Description standards at our facilities in Houston, Founded in 1968, VICI is an international group of Texas, USA, and Schenkon, Switzerland. companies. Our products can be found in nearly every Our other manufacturing facilities include national laboratory, military facility, and university research VICI Metronics in Washington, VICI department around the globe. For 50 years VICI has driven Precision Sampling in Louisiana, and the field of chromatography with valves, fittings, detectors, VICI DBS in Italy. VICI Canada serves our and instruments for precision analytical, biomedical, and neighbor to the north from its offices in biocompatible applications. We also customize components Brockville, Ontario. and complete systems to improve chromatographic analyses.

Valco Instruments Co., Inc. Chief Chromatographic Techniques Supported (VICI) ⦁⦁ High Pressure Liquid Chromatography P.O. Box 55603 ⦁⦁ Liquid Chromatography Houston, TX 77255 ⦁⦁ Gas Chromatography TELEPHONE ⦁⦁ Flow Injection Analysis (800) 367-8424 ⦁⦁ Supercritical Fluid Chromatography FAX ⦁⦁ Gas Permeation Chromatography (713) 688-8106 ⦁⦁ Process Gas Chromatography

E-MAIL [email protected] Major Products/Services VICI produces a complete line of valves for analytical chemistry. WEB SITE We specialize in zero dead volume fittings and filters for the www.vici.com separation sciences and are the original creators of the pulse NUMBER OF EMPLOYEES discharge detector (PDD). Our miniPDD consumes less than US: 365 one-fifth the amount of helium required by the PDDs used on Europe: 135 complete GC systems. It has similar sensitivity to the original YEAR FOUNDED PDD with a slightly lower dynamic range. 1968 Our VICI Metronics division produces PEEK, FEP, PFA, and ETFE tubing, ambient temperature gas purifiers, ValcoBond GC capillary columns, and Dynacal gas calibration standards. VICI 50 LCGC NORTH AMERICA CORPORATE CAPABILITIES December 2019 www.chromatographyonline.com Wyatt Technology

Major Products/Services WTC’s family of instruments include: ⦁⦁ DAWN: An 18-angle SEC-MALS instrument used at ambient, elevated, and below am- bient temperatures for polymers, particles, and biopolymers. ⦁⦁ miniDAWN: A SEC-MALS instrument ideal for proteins and peptides and molar mass materials below 1 million Da. ⦁⦁ microDAWN: The world’s first MALS in- strument for UHPLC. Compatible with all UHPLCs for molar mass and size determi- nations. ⦁⦁ Eclipse FFF system: For separation of mac- romolecules and nanoparticles in solution. ⦁⦁ Optilab: The most advanced RI detector on the market with 256 times the detection power and 50 times the dynamic range of any other RI detector. The Optilab can be operated below or above ambient tem- Company Description perature and determine dn/dc at the same Wyatt Technology, a 35-year old family-owned and operated wavelength of light as the light scattering enterprise, is the world’s largest company dedicated to SEC-MALS instrument. instruments for absolute macromolecular characterization. Our ⦁⦁ microOptilab: The world’s first refractive products provide techniques for characterizing macromolecules index (RI) detector for UHPLC. Operates and nanoparticles in solution, in order to determine molar mass, at the same wavelength of light as the size, charge, and interactions. Wyatt’s extensive applications microDAWN. laboratories and its full-time staff (including more than 30 PhD ⦁⦁ WyattQELS: A dynamic light-scattering scientists) means our customers receive deep technical expertise module integrated with the DAWN or and support. To ensure the personal touch, “Light Scattering miniDAWN to determine particle sizes as University” (LSU) is included with most instrument purchases. small as 1 nm. Wyatt Technology 6330 Hollister Ave., We bring you to Santa Barbara, CA, to demystify light scattering, ⦁⦁ ViscoStar viscometer: A state-of-the-art Santa Barbara, CA 93117, and explain how to get the most from your Wyatt Technology intrinsic viscosity instrument that can be USA equipment. Because we don’t just build instruments, we build operated above or below ambient tem- relationships. perature with its precise Peltier thermo- TELEPHONE static controls. (805) 681 9009 Chief Chromatographic Techniques Supported E-MAIL ⦁⦁ SEC & GPC Facility [email protected] ⦁⦁ UHPLC & APC WTC maintains more than 60,000 square WEB SITE ⦁⦁ Field-flow fractionation feet of R&D, manufacturing and application www.wyatt.com laboratories in Santa Barbara, California, USA.

YEAR FOUNDED Markets Served 1982 Wyatt’s products are the most widely used multi-angle light scattering (MALS) instruments in the world. They are employed in thousands of laboratories, including companies involved in biotechnology, pharmaceutical development, and chemical/petrochemical research, in addition to government national laboratories and academic facilities. www.chromatographyonline.com December 2019 LCGC NORTH AMERICA CORPORATE CAPABILITIES 51

maceutical, industrial chemical, and envi- YMC America, Inc. ronmental customers in North and South America.

Major Products/Services ⦁⦁ YMC-Triart ⦁⦁ YMC-BioPro ⦁⦁ Meteoric Core (Core-shell) ⦁⦁ Chiral, CHIRAL ART: Amylose-C, Amylose-C Neo, Cellulose-C, Amylose-SA, Cellulose-SB, Cellulose-SC; Cellulose-SJ; YMC Chiral Series: NEA (R), (S), α-CD BR, β-CD BR, γ-CD BR ⦁⦁ Reversed-phase, ODS: Reversed- phase, ODS: YMC-Triart C18, C18 ExRS, and Bio C18; Pro Series: Pro C18, Hy- drosphere C18, Pro C18 RS; YMC-Pack ODS Series: ODS-A, ODS-AM, ODS-AQ, ODS-AL; J’sphere ODS: ODS-H80, ODS- M80, ODS-L80; YMC-Pack PolymerC18 ⦁⦁ Other than ODS: YMC-Triart Bio C4 Company Description YMC-Triart C8, Phenyl, and PFP; Meteoric YMC America, Inc. is the exclusive sales and support channel for Core C8; Pro C8; Pro C4; YMCbasic, YMC separations leader YMC Co., Ltd., whose products include station- Carotenoid; YMC-Pack Series: C8, C4, ary phase chemistries primarily used for high and low pressure TMS, Ph (Phenyl), CN (Cyano), Protein-RP, separation science. Stationary phases designed for use with YMCbasic, YMC Carotenoid high performance liquid and supercritical fluid chromatography ⦁⦁ Normal phase: YMC-Triart Diol-HILIC; techniques are available as stocked standard items, and custom YMC-Pack Series: SIL, SIL-06, Diol-NP, products are available for specialized application needs. Products CN, PVA-Sil, Polyamine II, NH2, PA-G bonded to spherical silica, hybrid silica, and polymeric-based par- ⦁⦁ Ion-Exchange, YMC-BioPro Series: ticles ranging in size from 1.9 to 150 µm are available in packed QA (porous quaternary amine anion columns or in bulk chemical packaging formats. YMC America exchange), SF (porous sulfopropyl cation maintains offices, application support laboratories, and a ware- exchange), QA-F (non-porous QA), SP-F house in Allentown, Pennsylvania, and manages the applications (non-porous SF) support and sub-distribution of YMC products throughout the ⦁⦁ Size-Exclusion, YMC-Pack Series: Western hemisphere, either through direct contact with its cus- Diol-60, Diol-120, Diol-200, Diol-300; YMC America, Inc. tomers or via a network of distributors throughout the Americas. YMC-SEC MAB, YMC-GPC 941 Marcon Blvd. YMC products are available on a world-wide basis. ⦁⦁ Hydrophobic Interaction: YMC-BioPro Suite 201 HIC BF Allentown, PA 18109 Chief Chromatographic Techniques Supported In addition to all of YMC’s analytical chroma-

TELEPHONE ⦁⦁ HPLC tography offerings, most chromatography (610) 266-8650 ⦁⦁ UHPLC chemistries are scalable to bulk packing ma- ⦁⦁ Supercritical fluid chromatography (SFC) terials for lab-scale and production-scale puri- FAX ⦁⦁ Chiral chromatography fications. YMC also offers preparative systems (610) 266-8652 ⦁⦁ Hydrophobic interaction chromatography (HIC) for discovery, pilot-scale, and production. E-MAIL ⦁⦁ Ion-exchange (biomolecules) chromatography [email protected] ⦁⦁ Size-exclusion chromatography (SEC) Facilities WEB SITE ⦁⦁ Preparative chromatography YMC America, Inc. – Allentown, Pennsylvania www.ymcamerica.com ⦁⦁ HILIC chromatography YMC Co., Ltd. – Kyoto, Tokyo, Japan

NUMBER OF EMPLOYEES ⦁⦁ Superficially porous particle (core-shell) chromatography YMC Europe GmbH – Dinslaken, Germany Worldwide: 350 ⦁⦁ Nano–capillary chromatography YMC India Pvt. Ltd. – New Delhi, India ⦁⦁ Preparative chromatography systems YMC China – Shanghai, China YEAR FOUNDED ⦁⦁ Flow reactor chemistry YMC Korea Co., Ltd. – Gyeonggi-do, Korea 1980 YMC Taiwan Co., Ltd. – Taipei City, Taiwan Markets Served YMC Singapore Tradelinks Pte, Ltd. – Located in Allentown, Pennsylvania, YMC America, Inc., provides Singapore sales and service to pharmaceutical, biotechnology, biophar- YMC Schweiz GmbH – Basel, Switzerland MEDICAL/BIOLOGICAL

Analysis of Fentanyl and Its Analogues in Human Urine by LC–MS/MS Shun-Hsin Liang and Frances Carroll, Restek Corporation Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by LC–MS/MS using a Raptor Biphenyl column.

In recent years, the illicit use of synthetic opioids has 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 skyrocketed, and communities worldwide are now dealing Time (min) with an ongoing epidemic. Of the thousands of synthetic opioid overdose deaths per year, most are related to fentanyl Figure 1: The Raptor Biphenyl column effectively separated all target and its analogues. With their very high analgesic properties, compounds in urine with no observed matrix interferences. Peak elution order: norfentanyl-D , norfentanyl, remifentanil, acetyl fentanyl- synthetic opioid drugs such as fentanyl, alfentanil, remifentanil, 5 13C , acetyl fentanyl, alfentanil, fentanyl-D , fentanyl, carfentanil-D , and sufentanil are potent painkillers that have valid medical 6 5 5 carfentanil, butyryl fentanyl, sufentanil-D5, sufentanil. applications; however, they are also extremely addictive and are targets for abuse. In addition to abuse of these prescription chromatographic analysis using a Raptor Biphenyl column. This drugs, the current opioid crisis is fueled by a growing number method provides accurate, precise identification, and quantitation of illicit analogues, such as acetyl fentanyl and butyryl fentanyl, of fentanyl and related compounds, making it suitable for a variety which have been designed specifically to evade prosecution by of testing applications, including clinical toxicology, forensic drug enforcement agencies. analysis, workplace drug testing, and pharmaceutical research. As the number of opioid drugs and deaths increases, so does the need for a fast, accurate method for the simultaneous analysis Experimental Conditions of fentanyl and its analogues. Therefore, we developed this LC– MS/MS method for measuring fentanyl, six analogues, and one Sample Preparation metabolite (norfentanyl) in human urine. A simple dilute-and-shoot The analytes were fortified into pooled human urine. An 80 µL sample preparation procedure was coupled with a fast (3.5 min) urine aliquot was mixed with 320 µL of 70:30 water–methanol so- lution (fivefold dilution) and 10 µL of internal standard Table I: Analyte transitions (40 ng/mL in methanol) in a Thomson SINGLE StEP fil- ter vial (Restek cat. #25895). After filtering through the Precursor Product Ion Product Ion Analyte Internal Standard Ion Quantifier Qualifier 0.2 µm PVDF membrane, 5 µL was injected into the LC–MS/MS. Norfentanyl 233.27 84.15 56.06 Norfentanyl-D5 Acetyl fentanyl 323.37 188.25 105.15 Acetyl fentanyl-13C 6 Calibration Standards Fentanyl 337.37 188.26 105.08 Fentanyl-D 5 and Quality Control Samples Butyryl fentanyl 351.43 188.20 105.15 Carfentanil-D 5 The calibration standards were prepared in pooled hu- Remifentanil 377.37 113.15 317.30 Norfentanyl-D 5 man urine at 0.05, 0.10, 0.25, 0.50, 1.00, 2.50, 5.00,

Sufentanil 387.40 238.19 111.06 Sufentanil-D5 10.0, 25.0, and 50.0 ng/mL. Three levels of QC samples

Carfentanil 395.40 113.14 335.35 Carfentanil-D5 (0.75, 4.0, and 20 ng/mL) were prepared in urine for 13 Alfentanil 417.47 268.31 197.23 Acetyl fentanyl- C6 testing accuracy and precision with established cali- bration standard curves. Recovery analyses were per- Norfentanyl-D5 238.30 84.15 — — 13 formed on three different days. All standards and QC Acetyl fentanyl- C6 329.37 188.25 — — samples were subjected to the sample preparation pro- Fentanyl-D 342.47 188.27 — — 5 cedure described. Sufentanil-D 392.40 238.25 — — 5 LC–MS/MS analysis of fentanyl and its analogues was Carfentanil-D 400.40 340.41 — — 5 performed on an ACQUITY UPLC instrument coupled

52 THE APPLICATION NOTEBOOK – DECEMBER 2019 MEDICAL/BIOLOGICAL

with a Waters Xevo TQ-S mass spectrometer. Instrument conditions to 50 ng/mL for fentanyl, alfentanil, acetyl fentanyl, butyryl fen- were as follows, and analyte transitions are provided in Table I. tanyl, and sufentanil; from 0.10 to 50 ng/mL for remifentanil; and from 0.25 to 50 ng/mL for norfentanyl and carfentanil. All analytes Analytical column: Raptor Biphenyl (5 µm, showed acceptable linearity with r2 values of 0.996 or greater and 50 mm × 2.1 mm; cat. #9309552) deviations of <12% (<20% for the lowest concentrated standard). Guard column: Raptor Biphenyl EXP guard column cartridge, (5 µm, 5 mm × 2.1 mm; Accuracy and Precision cat. #930950252) Based on three independent experiments conducted on multiple Mobile phase A: 0.1% Formic acid in water days, method accuracy for the analysis of fentanyl and its ana- Mobile phase B: 0.1% Formic acid in methanol logues was demonstrated by the %recovery values, which were Gradient Time (min) %B within 10% of the nominal concentration for all compounds at all 0.00 30 QC levels. The %RSD range was 0.5–8.3% and 3.4–8.4% for in- 2.50 70 traday and interday comparisons, respectively, indicating accept- 2.51 30 able method precision (Table II). 3.50 30 Flow rate: 0.4 mL/min Conclusions Injection volume: 5 µL A simple dilute-and-shoot method was developed for the quantita- Column temp.: 40 °C tive analysis of fentanyl and its analogues in human urine. The Ion mode: Positive ESI analytical method was demonstrated to be fast, rugged, and sen- sitive with acceptable accuracy and precision for urine sample Results analysis. The Raptor Biphenyl column is well suited for the analy- Chromatographic Performance sis of these synthetic opioid compounds, and this method can All eight analytes were well separated within a 2.5-min gradient be applied to clinical toxicology, forensic analysis, workplace drug elution (3.5-min total analysis time) on a Raptor Biphenyl column testing, and pharmaceutical research. (Figure 1). No significant matrix interference was observed to neg- atively affect quantification of the fivefold diluted urine samples. The 5-µm particle Raptor Biphenyl column used here is a superfi- cially porous particle (SPP) column. It was selected for this meth- od in part because it provides similar performance to a smaller particle size fully porous particle (FPP) column, but it generates less system back pressure.

Linearity Linear responses were obtained for all compounds and the calibra- Restek Corporation tion ranges encompassed typical concentration levels monitored 110 Benner Circle, Bellefonte, PA 16823 for both research and abuse. Using 1/x weighted linear regression tel. 1 (814) 353-1300 (1/x2 for butyryl fentanyl), calibration linearity ranged from 0.05 Website: www.restek.com

Table II: Accuracy and precision results for fentanyl and related compounds in urine QC samples

QC Level 1 (0.750 ng/mL) QC Level 2 (4.00 ng/mL) QC Level 3 (20.0 ng/mL)

Average Conc. Average % Average Conc. Average % Average Conc. Average % Analyte %RSD %RSD %RSD (ng/mL) Accuracy (ng/mL) Accuracy (ng/mL) Accuracy Acetyl fentanyl 0.761 102 1.54 3.99 99.7 2.08 19.9 99.3 0.856 Alfentanil 0.733 97.6 3.34 3.96 98.9 8.38 20.9 104 6.73 Butyryl fentanyl 0.741 98.9 6.29 3.77 94.3 6.01 20.8 104 4.95 Carfentanil 0.757 101 7.34 3.76 94.0 4.64 20.6 103 4.24 Fentanyl 0.761 102 1.98 3.96 99.1 2.31 19.9 99.6 1.04 Norfentanyl 0.768 103 6.50 4.04 101 1.84 20.1 101 2.55 Remifentanil 0.765 102 3.42 3.97 99.2 3.68 20.8 104 4.14 Sufentanil 0.752 100 1.67 3.93 98.3 1.28 20.1 100 0.943

THE APPLICATION NOTEBOOK – DECEMBER 2019 53 FOOD/BEVERAGE

Separation of Selected Components of the Allergens Contained in Balsam of Peru Adam L. Moore, PhD, Hamilton Company

The essential oil balsam of Peru is commonly found in a multitude of products, including, but not limited to, perfumes, cosmetics, sunscreens, and flavored beverages. The fragrant 0.9 liquid is harvested from trees grown in Central America, and 0.8 1 2 smells richly of cinnamon, vanilla, and clove. The oil has been 0.7 used in three main areas: as a medicinal product pertaining 0.6 0.5 to its antiseptic, antifungal, and antiparasitic natures; as a 5 0.4 flavor enhancer for food and drinks; and as a fragrance in AU 0.3 4 perfumes and toiletries. Despite the utility of the mixture, it 0.2 3 has been shown that allergies can arise from some of the 0.1 individual components. Common symptoms generally present 0 -0.1 1 2 3 4 5 6 in the form of contact dermatitis and eczema. Use of such 0 Time (minutes) components in the manufacturing of the above mentioned Compounds: products requires quantification of the individual compounds. 1: Benzyl Alcohol 3: Farnesol 5: Benzyl Cinnaminate Therefore, we have developed an HPLC method that separates 2: Trans-Cinnamaldehyde 4: Benzyl Benzoate five components known to have allergenic properties. The method can be used with either the PRP-C18 (5 μm) or the Figure 1: PRP-1 separation of esters in balsam of Peru PRP-1 (5 μm) column. The two chromatograms illustrate the versatility and selectivity of the PS-DVB backbone found in all of Hamilton Company columns. 0.6 1 0.5

0.4 2

0.3 AU 5 0.2 4 0.1 3 0 0 1 2 3 4 5 6 7 8 Time (minutes)

Column Information Compounds: 1: Benzyl Alcohol 3: Farnesol 5: Benzyl Cinnaminate Packing Material Dimensions Part Number 2: Trans-Cinnamaldehyde 4: Benzyl Benzoate PRP-1 (5 μm) 150 x 4.6 mm 79641 PRP-C18 (5 μm) 150 x 4.6 mm 79676 Figure 2: PRP-C18 separation of esters in balsam of Peru Chromatographic Conditions 0.00–2 min, 75% B Gradient 2.01–5 min, 75–100% B 5.01–8 min, 100% B Temperature Ambient Injection Volume 10 μL Detection UV at 214 mm Eluent A Water Eluent B Acetonitrile Hamilton Company, Inc. 2.0 mL/min (PRP-1) Flow Rate 4970 Energy Way, Reno, NV 89502 2.5 mL/min (PRP-C18) tel. (775)-858-3000, (800) 648-5950 Website: www.hamiltoncompany.com

54 THE APPLICATION NOTEBOOK – DECEMBER 2019 FOOD/BEVERAGE

Shodex Rapid SUGAR Series: Fast Fermentation Monitoring and Sourdough Cultures Alexander Schrum, Ronald Benson, and Marlon Rettis,  Showa Denko America, Inc.

Living organisms (yeast and bacteria) were not identified as the agents that produced or spoiled common food items until the 19th century. Bak- ing, especially of bread, was among the first industries to be transformed by the emerging scientific understanding of fermentation. The introduc- tion of commercialized yeast in the early 20th century steadily reduced Figure 1: RI chromatogram of fermentation standards, 0.1% w/v the time needed to bake bread both at home and on the production each, 5 uL injection. line, making way for modern packaged breads (1). However, traditional fermentation techniques, including sourdough breads, have resurged in popularity. Typical sourdough cultures are created from yeast and bacte- ria populations naturally present in the air and on many surfaces, provid- ing sourdough loaves with their characteristic rise and “tangy” flavor by converting fermentable sugars into CO2 and flavor compounds like lactic acid. In this application note, a “homemade” sourdough starter culture was analyzed to determine how key fermentation products changed over the course of 24 h with the new Rapid SUGAR SH1011 8C column. The Shodex™ Rapid SUGAR SH1011 8C column contains a styrene Figure 2: Sourdough timepoints: Pink is time 0, green is 12 h, black is 24 h. Peak 1: glucose and sample matrix. Peak 2: lactic acid. Peak 3: divinylbenzene base material ligand exchange column, and is designed glycerol. Peak 4: ethanol for rapid fermentation monitoring, allowing for the analysis of saccha- rides, organic acids, and ethanol in less than 5 min. The method uses Results and Discussion simple aqueous conditions and RID detection, ideal for large sample This simple method using 1 mM sulfuric acid as the eluent demon- workflows and QC environments. strated a successful simulated analysis of fermentation byproducts in less than 5 min, including the column equilibration time. Experimental For the analysis of the sourdough starter culture, a guard column Seven common fermentation compounds (maltotriose, maltose, was used: Shodex™ SUGAR SH-G (6.0 × 50 mm id, 10 μm) (Figure 2). glucose, lactic acid, acetic acid, glycerol, and ethanol) were used as A method for rapid fermentation monitoring was developed us- standards. The sourdough starter culture was adapted from King Arthur ing the Shodex SUGAR SH1011 8C column. Fermentation sample (2), with the procedure repeated daily for two weeks. measurement was completed in 6 min. The column showed good robustness when used with complex sample matrices like sourdough Sample preparation cultures. The results show that the SH1011 8C column can be used Approximately 10 g of sample was homogenized with 90 mL of distilled to monitor fermentation products like organic acids and ethanol. water (for 30 s, with the blender on maximal speed). Five milliliters of 1 mol/L HClO4 solution was added to a 10 mL aliquot of the homogenate. References The mixture was centrifuged for 15 min at 4000 g at 15 1C, the superna- (1) J. Marx, J.H. Litchfield, A Revolution in Biotechnology (Cambridge University tant was neutralized (pH 7.0) with 2 mol/L KOH, and the volume was ad- Press, 1989). justed to 25 mL with distilled water. After 30 min precipitation on ice, the (2) The King Arthur Flour Baker’s Compan- solution was filtered on 0.45 mm cellulose filter, adapted from reference 3. ion: The All-Purpose Baking Cookbook Samples were taken starting on Day 15 at three time points: (The Countryman Press, 2012) Sample 1: Immediately after mixing in flour and water, labelled time 0 (3) D. Lefebvre, V. Gabriel, Y. Vayssier, and Sample 2: 12 h after time 0 C. Fontagné-Faucher, Food Sci. Tech 35. Sample 3: Day 16, approximately 24 after time 0 407–414 (2002). Shodex SUGAR SH1011 8C (8.0 mm I.D. × 100 mm, 6 μm) was used with a Shodex RI-501 detector. The eluent conditions were: 1 mM Shodex™/Showa Denko America, Inc.

H2SO4 aq. The column was kept at 75 ºC, and the flow rate was 1.0 mL/ 420 Lexington Avenue Suite 2335A, New York, NY, 10170 min. The chromatography was performed on a Shimadzu Prominence tel. (212) 370-0033, X116, fax: (212) 370-4566 I series LC 2030 instrument. Website: www.shodexhplc.com

THE APPLICATION NOTEBOOK – DECEMBER 2019 55 PHARMA/DRUG

Maximum Resolution for Biomolecule Separation MilliporeSigma

MilliporeSigma ranges of protein and peptide columns have been specically engineered for fast, high-resolution separation of biomolecules. The Fused-Core® superficially porous particles (SPP) contained within BIOshell™ columns are strategically designed to

provide highly efficient separations of proteins and peptides 1 3 5 200 BIOshell® 160 Å 4 ™ 2 BIOshell UHPLC and HPLC columns deliver maximum speed 150 Peptide C18 100 6 and efficiency and the low backpressure makes them suitable for use 50 0 0 1 2 3 4 5 6 78 9 10 11 12 13 14 15 on both UHPLC and HPLC systems. The Fused-Core particles with -50 1 3 5 200 ® 4 BIOshell 160 Å 2 pore sizes from 90 Å to 1000 Å allow superior separation from glycans 150 Peptide CN 100 6 up to very large proteins. Optimum separation of peptides of <20 kDa 50 0 0 1 2 3 4 56 78 9 10 11 12 13 14 15 for peptide mapping and proteomic applications can be achieved -50 1 3 5 200 BIOshell® 160 Å 4 2 with BIOshell Peptide columns and for the separation of monoclonal Absorbance (mAU) 150 Peptide Phenyl-Hexyl 100 6 antibodies (mAbs) or proteins >100 kDa in biotherapeutic drug 50 0 0 1 2 3 4 56 78 9 10 11 12 13 14 15 development. BIOshell IgG columns with a pore size of 1000 Å show -50 Time (min) columns: BIOshell™ 160 Å Peptide C18, CN & Phenyl-Hexyl, 1. Tyr-Tyr-Tyr clear advantages over common 300 Å pores. 15 cm x 2.1 mm I.D., 2.7 μm 2. Angiotensin II mobile phase A: water/0.1 % TFA 3. Angiotensin-(1-12) mobile phase B: ACN/0.1 % TFA 4. Melittin 5. Sauvagine gradient: 0-60 % B in 15 min 6. β-Endorphin 0.4 mL/min temp: 60 °C detection: 220 nm BIOshell IgG 1000 Å C18 and BIOshell IgG 1000 Å Diphenyl injection: 2 μL These two new column materials expand the choices for selectivity, in addition to the well-established BIOshell IgG 1000 Å C4 phase, Figure 2: Separation of peptide mix. for maximum resolution in the separation of monoclonal antibodies, antibody–drug conjugates, antibody fragments, and large proteins with molecular weights ≤500 kDa.

BIOshell 160 Å Peptide Phenyl-Hexyl

The new BIOshell 160 Å Peptide Phenyl-Hexyl offers an alternative Fused-Core®

Fused-Core® selectivity to the C18 and CN phase chemistries of the BIOshell™ Fused-Core® Fused-Core®

1.2 μm 1.7 μm 3.0 μm 3.3 μm

160 Å Peptide column line for peptide mapping and proteomic 0.4 μm 0.5 μm 0.2 μm 0.6 μm

2.0 μm 2.7 μm 3.4 μm 5 μm applications requiring high-resolution separation of peptides and USP Pore Max Code Size Temp.* 2 μm 2.7 μm 3.4 μm 5 μm polypeptides <20 kDa. Glycan L95 X

Peptide C18 L1 160 Å X X X

Peptide CN L10 160 Å X X For more information, visit SigmaAldrich.com/BIOshell Peptide Phenyl-Hexyl L11 160 Å X Protein C18 L1 400 Å X

Protein C4 L26 400 Å X

lgG C18 L1 1000 Å X

lgG C4 L26 1000 Å X

lgG Diphenyl L11 1000 Å X

*at low pH.

BIOshell® 1000 Å lgG C4 ® 40000 BIOshell 1000 Å Diphenyl Figure 3: BIOshell UHPLC and HPLC columns. BIOshell® 1000 Å C18 30000

UV 20000

10000

0 4 6 8 10 12 14 16 Time columns: BIOshell™ IgG 1000 Å C4, 15 cm x 2.1 mm I.D., 2.7 μm (min) Bioshell™ IgG 1000 Å C18, 15 cm x 2.1 mm I.D., 2.7 μm BIOshell™ IgG 1000 Å Diphenyl, 15 cm x 2.1 mm I.D., 2.7 μm 0.4 mL/min mobile phase A: water/0.1 % TFA mobile phase B: ACN/0.1 % TFA gradient: 32-40 % B in 16 min inj. vol.: 2 μL of 2 mg/mL trastuzumab in water/0.1% TFA MilliporeSigma temp: 80 °C detection: PDA at 280 nm 400 Summit Drive, Burlington, MA 01803 tel. (800) 645-5476 Figure 1: IgG separation on 1000 Å SPP particles. Website: SigmaAldrich.com

56 THE APPLICATION NOTEBOOK – DECEMBER 2019 PHARMA/DRUG

Amino Acid Analysis According to European Pharmacopoeia 8.0 Maria Ofitserova, Sareeta Nerkar, Pickering Laboratories, Inc.

The European Pharmacopoeia (Ph. Eur.) defines requirements for Post-column reagent the qualitative and quantitative composition of medicines, as well as • Trione® Ninhydrin Reagent the tests to be carried out on medicines and on the substances and To make it easier to start using Pickering Laboratories methods, we materials used in their production. The European Pharmacopoeia offer chemistry kits that include: analytical column, GARD, buffers, and its requirements are legally binding in the member states of reagents, and standards for amino acids analysis. All parts of the the European Pharmacopoeia Convention and the European Union. kit can be ordered individually if needed. Please contact Pickering Pickering Laboratories, Inc. offers a complete solution for Amino Laboratories if you have any questions regarding this application. acids analysis according to European Pharmacopoeia 8.0. This includes the Onyx PCX post-column derivatization instrument, analytical columns and GARDs, buffers, and Trione® Ninhydrin reagents. The Onyx PCX is capable of performing column temperature gradients that allow easily modified conditions and improved run times, and amino acids separations. The methods presented in this application note were optimized to comply with the system-suitability requirements of Europoean Pharmacopoeia 8.0 methods. Ammonia

Equipment Lysine • Quaternary HPLC pump, autosampler, UV–vis detector Threonine • Onyx PCX post-column derivatization system -- Valine Methionine Isoleucine Leucine Phenylalanine Analytical columns and eluants Proline -- • For sodium-based methods: High-efficiency sodium cation- -- exchange column, 4.6 x 110 mm, catalog number 1154110T. 0 10 20 30 ------40 ------min ------Eluants: Na315, Na425, Na640, RG011. • For lithium-based methods: High-efficiency lithium cation- exchange column, 4.6 x 75 mm, catalog number 0354675T. Figure 2: Sodium chromatogram of alternative amino acids analyzed Eluants: 1700-1125, Li365, Li375, RG003. using Ph. Eur. 8.0 methods (3 ug/mL each, 50 uL injection). Ammonia Ammonia Leucine Isoleucine Cystine Serine Proline Alanine Cysteine Lysine Threonine -- 20 0 10 30 40 50 min Valine Isoleucine Leucine Cystine Arginine Figure 3: Lithium chromatogram of amino acids used as reference Proline -- -- solutions for cysteine analysis (3 ug/mL each, 50 uL injection).

min 0 10 20 30 40 Pickering Laboratories, Inc. 1280 Space Park Way, Mountain View, CA 94043 Figure 1: Sodium chromatogram of amino acids analyzed using Ph. tel. (800) 654-3330, (650) 694-6700, [email protected] Eur. 8.0 methods (3 ug/mL each, 50 uL injection). Website: www.pickeringlabs.com

THE APPLICATION NOTEBOOK – DECEMBER 2019 57 POLYMER

Evolved Gas Analysis and Multi-Step Pyrolysis: Two Powerful Tools for Polymer Analysis Karen Sam, CDS Analytical

The plastics and rubber industry plays a significant role in bringing us to the modern age, which emphasizes the balance between life convenience and environmental sustainability. From the very beginning of this industry, four different categories of additives were incorporated into these­ products as stabilizers, plasticizers, lubricants and flame retardants to either enhance the physical performance of the finished product or assist the manufacturing process. A vast number of chemical species are being used as polymer additives nowadays. The molecular weight varies from 200 to 1000 Da with a wide range of boiling points. These additives can become a major challenge in the quality control process. A single-step pyrolysis GC–MS analysis often produces cluttered chromatograms without adequate separation within residual solvents, pyrolysis fragments, contaminants, and additives. Evolved gas analysis (EGA), in conjunction with multi- step pyrolysis, can address this challenge effectively. EGA provides Figure 1: EGA plots of clear vinyl (left) and green vinyl (right), TICs thermal information that can be used to select appropriate multi-step (bottom), and EICs. thermal extraction temperatures. Each thermally sliced chromatogram provides ample separation to yield well-resolved chemical information.

Experimental Setup A clear vinyl transparency sheet underwent EGA at 50 °C per min from 50 °C to 800 °C. This temperature ramp up rate is 2.5 times faster than the ramp up of traditional thermal gravimetric analysis (TGA). A short- fused silica column was used. Approximately 100 µg of vinyl polymer was added to the drop-in-sample Chamber (DISC) tube, and analyzed with a CDS 6150 Pyroprobe, which was connected to a GC–MS system. Pyroprobe Setpoints: Initial: 50 °C Figure 2: Clear Vinyl at 250 °C (top), 350 °C(middle) and 500 °C (bottom). Final: 800 °C for HCl. This represents the first step of polyvinyl chloride pyrolysis: Ramp Rate: 50 °C per min removal of the chlorine side group. The last region has multiple masses DISC Interface: 300 °C associated with it; m/z = 91, 106, and 115 indicate aromatics, and Transfer Line: 300 °C represent the stabilization of the remaining polymer chain. Valve Oven: 300 °C After obtaining the EGA information, 250 °C, 350 °C, and 500 °C GC-MS were chosen to separate semi-volatiles and polymeric components Column: fused silica (1m x 0.10mm) for GC–MS analysis. Dioctyl phthalate (Figure 2) was identified in the Carrier: helium 1.25mL/min, 75:1 split 250 °C thermal slice. At 400 °C, dioctyl phthalate is still extracting Oven: isothermal 300 °C as the vinyl breaks down, releasing HCl. At 500 °C, the remaining Ion Source: 230 °C portion of the vinyl are stabilized to aromatics. Mass Range: 35–600 amu Conclusion Results Evolved gas analysis (EGA) in conjunction with multi-step pyrolysis can Figure 1, which displays the EGA from the transparency sheet, including be used to effectively determine multi-step pyrolysis parameters, simpli- a total ion chromatogram (TIC), and Extracted Ion Chromatograms (EIC), fying chromatography results of complex polymeric matrices. has three defined regions of thermal outgassing. These peak regions occur at 250 °C, 350 °C, and 500 °C. The first region is contributed by CDS Analytical semi-volatiles (m/z = 149) from phthalate plasticizers desorbed from 465 Limestone Road, Oxford, PA 19363 the vinyl. The second and third regions are from material decomposition. tel. (800) 541-6593 The second region has an abundance of m/z = 36 with a top match Website: www.cdsanalytical.com

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