Anti-ERO1L Antibody (ARG42132)

Product datasheet [email protected]

ARG42132 Package: 50 μg anti-ERO1L antibody Store at: -20°C

Summary

Product Description Goat Polyclonal antibody recognizes ERO1L

Tested Reactivity Hu

Predict Reactivity Ms, Rat, Cow, Dog, Pig

Tested Application IHC-P, WB

Host Goat

Clonality Polyclonal

Isotype IgG

Target Name ERO1L

Antigen Species Human

Immunogen Synthetic peptide around the internal region of Human ERO1L. (C-QSDEVPDGIKSASY) (NP_055399.1)

Conjugation Un-conjugated

Alternate Names ERO1-like protein alpha; ERO1L; Endoplasmic oxidoreductin-1-like protein; ERO1-alpha; ERO1-L-alpha; Ero1alpha; Oxidoreductin-1-L-alpha; EC 1.8.4.-; ERO1LA; ERO1-L

Application Instructions

Application table Application Dilution

IHC-P 5 µg/ml

WB 0.1 - 0.3 µg/ml

Application Note WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control A431

Calculated Mw 54 kDa

Observed Size ~ 70 kDa

Properties

Form Liquid

Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.

Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.

Preservative 0.02% Sodium azide

Stabilizer 0.5% BSA www.arigobio.com 1/2 Concentration 0.5 mg/ml

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol ERO1A

Gene Full Name endoplasmic reticulum oxidoreductase alpha

Function Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. [UniProt]

Cellular Localization Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. Note=The association with ERP44 is essential for its retention in the endoplasmic reticulum. [UniProt]

Images

ARG42132 anti-ERO1L antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42132 anti-ERO1L antibody at 5 µg/ml dilution followed by AP-staining.

ARG42132 anti-ERO1L antibody WB image

Western blot: 35 µg of A431 cell lysate (in RIPA buffer) stained with ARG42132 anti-ERO1L antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.