Protein G Agarose
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Highly Efficient Purification of Protein Complexes from Mammalian Cells
Highly efficient purification of protein complexes from mammalian cells using a novel streptavidin-binding peptide and hexahistidine tandem tag system: Application to Bruton’s tyrosine kinase Yifeng Li,1 Sarah Franklin,1 Michael J. Zhang,1 and Thomas M. Vondriska1,2,3* 1Department of Anesthesiology, David Geffen School of Medicine, University of California, Los Angeles, CA 2Department of Medicine/Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA 3Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA Received 2 June 2010; Revised 9 September 2010; Accepted 27 October 2010 DOI: 10.1002/pro.546 Published online 15 November 2010 proteinscience.org Abstract: Tandem affinity purification (TAP) is a generic approach for the purification of protein complexes. The key advantage of TAP is the engineering of dual affinity tags that, when attached to the protein of interest, allow purification of the target protein along with its binding partners through two consecutive purification steps. The tandem tag used in the original method consists of two IgG-binding units of protein A from Staphylococcus aureus (ProtA) and the calmodulin- binding peptide (CBP), and it allows for recovery of 20–30% of the bait protein in yeast. When applied to higher eukaryotes, however, this classical TAP tag suffers from low yields. To improve protein recovery in systems other than yeast, we describe herein the development of a three-tag system comprised of CBP, streptavidin-binding peptide (SBP) and hexa-histidine. We illustrate the application of this approach for the purification of human Bruton’s tyrosine kinase (Btk), which results in highly efficient binding and elution of bait protein in both purification steps (>50% recovery). -
Analysis of Proteins by Immunoprecipitation
Laboratory Procedures, PJ Hansen Laboratory - University of Florida Analysis of Proteins by Immunoprecipitation P.J. Hansen1 1Dept. of Animal Sciences, University of Florida Introduction Immunoprecipitation is a procedure by which peptides or proteins that react specifically with an antibody are removed from solution and examined for quantity or physical characteristics (molecular weight, isoelectric point, etc.). As usually practiced, the name of the procedure is a misnomer since removal of the antigen from solution does not depend upon the formation of an insoluble antibody-antigen complex. Rather, antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody (Figure 1). Thus, unlike other techniques based on immunoprecipitation, it is not necessary to determine the optimal antibody dilution that favors spontaneously-occurring immunoprecipitates. Figure 1. Schematic representation of the principle of immunoprecipitation. An antibody added to a mixture of radiolabeled (*) and unlabeled proteins binds specifically to its antigen (A) (left tube). Antibody- antigen complex is absorbed from solution through the addition of an immobilized antibody binding protein such as Protein A-Sepharose beads (middle panel). Upon centrifugation, the antibody-antigen complex is brought down in the pellet (right panel). Subsequent liberation of the antigen can be achieved by boiling the sample in the presence of SDS. Typically, the antigen is made radioactive before the immunoprecipitation procedure, either by culturing cells with radioactive precursor or by labeling the molecule after synthesis has been completed (e.g., by radioiodination to iodinate tyrosine residues or by sodium [3H]borohydride reduction to label carbohydrate). -
Magresyn ® Protein G
MagReSyn® Protein G Immobilized Protein G magnetic 1.4. Additional Equipment and Materials microparticles Magnetic separator, Vortex mixer, Buffers and solutions, end-over-end mixer (optional) Ordering Information Cat. No. Quantity 2. Immunoglobulin Purification Factors that may affect the attachment of antibodies include the isotype of the MR-PRG002 2 ml immunoglobulin, buffer composition and pH, and the presence of MR-PRG005 5 ml contaminants/interfering compounds. The quantity of microparticles needs to be optimized for each individual application. We recommend the application of excess MR-PRG010 2 x 5 ml ligand to ensure saturation of the Protein G microparticles. The binding efficiency can be determined by comparing the ligand concentration before and after coupling. This product is for research use only MagReSyn® Protein G is compatible with various commonly used buffers, including Tris and Phosphate. Recommended buffers include: Binding/wash buffer - TBS (50 mM Tris pH 7.5, 150 mM NaCl, 0.025% Tween® 20) or PBS (50 mM Phosphate pH 7.5, 150 mM Table of Contents: NaCl, 0.025% Tween® 20); Elution Buffer (Native): 0.1 M glycine pH 2.5 or 2.5% acetic 1. Product Description acid; Elution Buffer (Denaturing): SDS-PAGE electrophoresis buffer. 2. Immunoglobulin Purification 3. Immunoprecipitation NOTE: All reagents should be freshly prepared and of analytical grade to ensure 4. Recommended Storage optimal performance. The procedures, methods and buffer solutions described below serve as an example and are not intended to be limiting. MagReSyn® Protein G is 5. Antibody Binding Guide compatible with a range of different buffers for binding of antibodies. -
6511-Protein G-Sepharose
FOR RESEARCH USE ONLY! Protein G-Sepharose rev. 09/16 ° Store at 4 C. Do not freeze. Cat. No. 6511-1 Protein G-Sepharose, 1 ml settled resin 6511-5 Protein G-Sepharose, 5 ml settled resin 6511-25 Protein G-Sepharose, 25 ml settled resin 6511-100 Protein G-Sepharose, 100 ml settled resin 6511-1000 Protein G-Sepharose, 1 L settled resin Support: 6% cross-linked Sepharose beads supplied as 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% Ethanol/H2O. Binding Capacity: >20 mg human or rabbit IgG/ml of settled resin. Flow Rate Tested*: 0.85 cm/min. *Test condition: Linear flow rate determined in 2 ml column with internal diameter of 1.5 cm. Introduction: Protein G is a cell wall protein produced by group G streptococcus. Like protein A, this bacteria-derived protein binds with high affinity & specificity to the Fc portion of most mammalian immunoglobulins. Therefore, Protein G has been widely used for IgG purification. BioVision’s Protein G (Cat. # 6510) is a genetically engineered protein containing three Ig-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. In addition, Protein G-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. -
Structural and Functional Studies on Photoactive Proteins and Proteins Involved in Cell Differentiation
Technische Universität München Fakultät für Organishe Chemie und Biochemie Max-Planck-Institut für Biochemie Abteilung Strukturforschung Biologische NMR-Arbeitsgruppe STRUCTURAL AND FUNCTIONAL STUDIES ON PHOTOACTIVE PROTEINS AND PROTEINS INVOLVED IN CELL DIFFERENTIATION Pawel Smialowski Vollständiger Abdruck der von der Fakultät für Chemie der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Univ.-Prof. Dr. Dr. A. Bacher Prüfer der Dissertation: 1. apl. Prof. Dr. Dr. h.c. R. Huber 2. Univ.-Prof. Dr. W. Hiller Die Dissertation wurde am 10.02.2004 bei der Technischen Universität München eingereicht und durch die Fakultät für Chemie am 17.03.2004 angenommen. PUBLICATIONS Parts of this thesis have been or will be published in due course: Markus H. J. Seifert, Dorota Ksiazek, M. Kamran Azim, Pawel Smialowski, Nedilijko Budisa and Tad A. Holak Slow Exchange in the Chromophore of a Green Fluorescent Protein Variant J. Am. Chem. Soc. 2002, 124, 7932-7942. Markus H. J. Seifert, Julia Georgescu, Dorota Ksiazek, Pawel Smialowski, Till Rehm, Boris Steipe and Tad A. Holak Backbone Dynamics of Green Fluorescent Protein and the effect of Histidine 148 Substitution Biochemistry. 2003 Mar 11; 42(9): 2500-12. Pawel Smialowski, Mahavir Singh, Aleksandra Mikolajka, Narashimsha Nalabothula, Sudipta Majumdar, Tad A. Holak The human HLH proteins MyoD and Id–2 do not interact directly with either pRb or CDK6. FEBS Letters (submitted) 2004. ABBREVIATIONS ABBREVIATIONS -
Thermo Scientific Pierce Protein Purification Technical Handbook
Thermo Scientific Pierce Protein Purification Technical Handbook O O H H2N O N N + Version 2 O Bead Bead H2N Agarose O O NH2 NH2 Table of Contents Introduction for Protein Purification 1-5 Antibody Purification 58-67 Overview 58 Purification Accessories – Protein Extraction, Immobilized Protein L, Protein A, Protein G 59-61 and Protein A/G Binding and Elution Buffers 6-11 IgG Binding and Elution Buffers for 62 Protease and Phosphatase Inhibitors 8 Protein A, G, A/G and L for Protein Purification Melon Gel Purification Products 63 Buffers for Protein Purification 9 Thiophilic Gel Antibody Purification 64 Spin Cups and Columns 10 IgM and IgA Purification 65 Disposable Plastic and Centrifuge Columns 11 Avidin:Biotin Binding 66-69 Fusion Protein Purification 12-21 Biotin-binding Proteins 66 His-Tagged Protein Purification Resin 14-15 Immobilized Avidin Products 67 Cobalt Resin, Spin Columns and 16-17 Immobilized Streptavidin Products 67 Chromatography Cartridges Immobilized NeutrAvidin Products 68 High-quality purification of GST-fusion proteins 18-19 Immobilized Monomeric Avidin and Kit 68 GST- and PolyHis-Tagged Pull-Down Assay Kits 20-21 Immobilized Iminobiotin and Biotin 69 Covalent Coupling of Affinity Ligands FPLC Cartridges 70-77 to Chromatography Supports 22-35 Overview 70 Covalent Immobilization of Ligands 22-25 His-tagged Protein FPLC Purification 71-72 Products for Immobilizing Ligands 26-29 GST-Tagged Protein FPLC Purification 72 through Primary Amines Antibody FPLC Purification 73-75 Products for Immobilizing Ligands 30-32 through Sulfhydryl Groups Phosphoprotein FPLC Purification 75 Products for Immobilizing Ligands 33 Biotinylated Protein FPLC Purification 76 through Carbonyl Groups Protein Desalting 77 Products for Immobilizing Ligands 34-35 through Carboxyl Groups Affinity Supports 78-80 IP/Co-IP 36-45 Immunoprecipitation 36 Traditional Methods vs. -
A Predictive Model of Antibody Binding in the Presence of Igg-Interacting Bacterial Surface Proteins
bioRxiv preprint doi: https://doi.org/10.1101/2020.10.20.347781; this version posted October 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. A predictive model of antibody binding in the presence of IgG-interacting bacterial surface proteins Vibha Kumra Ahnlide1, Therese de Neergaard1, Martin Sundwall1, Tobias Ambjörnsson2, and Pontus Nordenfelt1 1Division of Infection Medicine, Department of Clinical Sciences, Faculty of Medicine, Lund University, Lund, Sweden 2Computational Biology and Biological Physics, Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden Many bacteria can interfere with how antibodies bind to their surfaces. This bacterial antibody targeting makes it challeng- According to our previous findings (22), the IgGFc-binding ing to predict the immunological function of bacteria-associated of M and M-like proteins is more prominent in low IgG antibodies. The M and M-like proteins of group A streptococci concentration environments, corresponding to that of the exhibit IgGFc-binding regions, which they use to reverse IgG human throat milieu. At the higher IgG concentrations in binding orientation depending on the host environment. Unrav- plasma, IgG is mostly bound to the surface proteins via eling the mechanism behind these binding characteristics may identify conditions under which bound IgG can drive an effi- Fab. This suggests that the antibody binding orientation is cient immune response. Here, we have developed a biophysi- dependent on the IgG concentration. -
Chapter 2. Materials and Methods 2 Materials and Methods 2.1
Chapter 2. Materials and Methods 20 2 Materials and Methods 2.1 Materials 2.1.1 Apparatus FLA-3000 Fuji-Film FPLC Amersham Pharmacia Heraeus Biofuge Stratos, Centrifuges Kendro (Honau) LSM 510 Zeiss pH-meter Mettler Toledo GmbH SLM Aminco French pressure SLM Aminco Sorvall RC5B Plus, Centrifuge Kendro Thermo-block Bioblock Scientific Trio-Thermocycler Biometra 2.1.2 Chemicals Acrylamid/Bisacrylamid 37:5:1 Roth, Karlsruhe, Germany Acrylamid 30% Roth, Karlsruhe, Germany Agar AppliChem, Darmstadt, Germany Agarose Serva, Heidelberg, Germany Ammonium persulphate Roth, Karlsruhe, Germany Ampicilin Roche, Mannheim, Germany Boric Acid Sigma, St Loius, MO, USA Coomassie Brilliant Blue R G25 Serva, Heidelberg, Germany Dimetylsulphoxide Sigma, St Loius, MO, USA EDTA Serva, Heidelberg, Germany EGTA Serva, Heidelberg, Germany β-Mercaptoethanol Sigma, St Loius, MO, USA Paraformaldehyde Sigma, St Loius, MO, USA Sodium fluoride Serva, Heidelberg, Germany TEMED Merck, Darmstadt, Germany Tris AppliChem, Darmstadt, Germany Trypsin Biochrom KG, Berlin, Germany Tween-20 Sigma, St Loius, MO, USA Triton X-100 Serva, Heidelberg, Germany Chapter 2. Materials and Methods 21 2.1.3 Standards and kits ECL Western Blotting Reagents Amersham Pharmacia, Uppsala, Sweden Coomassie Protein Assay kit Pierce, Illinois, USA Qiagen Plasmid Mini Kit Qiagen, Hilden The Netherlands Qiagen Plasmid Midi Kit Qiagen, Hilden, The Netherlands QIAquick Gel Extraction Kit Qiagen, Hilden, The Netherlands QIAquick PCR Extraction Kit Qiagen, Hilden, The Netherlands QIAquick Nucleotide Extraction -
A Tool to Detect Cis-Acting Elements Near Promoter Regions in Rice
Planta (2021) 253:40 https://doi.org/10.1007/s00425-021-03572-w ORIGINAL ARTICLE Rice protein‑binding microarrays: a tool to detect cis‑acting elements near promoter regions in rice Joung Sug Kim1 · SongHwa Chae1 · Kyong Mi Jun2 · Gang‑Seob Lee3 · Jong‑Seong Jeon4 · Kyung Do Kim1 · Yeon‑Ki Kim1 Received: 27 October 2020 / Accepted: 8 January 2021 / Published online: 21 January 2021 © The Author(s) 2021 Abstract Main conclusion The present study showed that a rice (Oryza sativa)-specifc protein-binding microarray (RPBM) can be applied to analyze DNA-binding motifs with a TF where binding is evaluated in extended natural promoter regions. The analysis may facilitate identifying TFs and their downstream genes and constructing gene networks through cis-elements. Abstract Transcription factors (TFs) regulate gene expression at the transcriptional level by binding a specifc DNA sequence. Thus, predicting the DNA-binding motifs of TFs is one of the most important areas in the functional analysis of TFs in the postgenomic era. Although many methods have been developed to address this challenge, many TFs still have unknown DNA- binding motifs. In this study, we designed RPBM with 40-bp probes and 20-bp of overlap, yielding 49 probes spanning the 1-kb upstream region before the translation start site of each gene in the entire genome. To confrm the efciency of RPBM technology, we selected two previously studied TFs, OsWOX13 and OsSMF1, and an uncharacterized TF, OsWRKY34. We identifed the ATT GAT TG and CCA CGT CA DNA-binding sequences of OsWOX13 and OsSMF1, respectively. In total, 635 and 932 putative feature genes were identifed for OsWOX13 and OsSMF1, respectively. -
Recombinant Human Igg4 Fc Protein
Leader in Biomolecular Solutions for Life Science Recombinant Human IgG4 Fc Protein Catalog No.: RP00801 Recombinant Sequence Information Background Species Gene ID Swiss Prot As a monomeric immunoglobulin that is predominately involved in the secondary Human P01861 antibody response and the only isotypethat can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, Tags andconstitutes 75% of serum immunoglobulins in humans. IgG antibodies protect No tag the body against the pathogens byagglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated Synonyms cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light Ig gamma-4 chain C region;IgG4 Fc chains (25 kDa) linked bydisulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fabfragment (antigen- binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum amongthe four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragmentis Product Information demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A onthe surface of certain Staphylococcal Source Purification and Streptococcal strains specifically binds with the Fc region of IgGs, and Human Cells > 95% by SDS- hasnumerous applications in biotechnology as a reagent for affinity purification. PAGE. Recombinant IgG Fc Region is suggested torepresent a potential anti- inflammatory drug for treatment of human autoimmune diseases. Endotoxin < 1 EU/μg of the protein by LAL Basic Information method. -
Analysis of the Biochemical and Cellular Activities of Substrate Binding by the Molecular Chaperone Hsp110/Sse1
The Texas Medical Center Library DigitalCommons@TMC The University of Texas MD Anderson Cancer Center UTHealth Graduate School of The University of Texas MD Anderson Cancer Biomedical Sciences Dissertations and Theses Center UTHealth Graduate School of (Open Access) Biomedical Sciences 5-2017 ANALYSIS OF THE BIOCHEMICAL AND CELLULAR ACTIVITIES OF SUBSTRATE BINDING BY THE MOLECULAR CHAPERONE HSP110/SSE1 Veronica M. Garcia Follow this and additional works at: https://digitalcommons.library.tmc.edu/utgsbs_dissertations Part of the Biochemistry Commons, Medicine and Health Sciences Commons, Microbiology Commons, Molecular Biology Commons, and the Molecular Genetics Commons Recommended Citation Garcia, Veronica M., "ANALYSIS OF THE BIOCHEMICAL AND CELLULAR ACTIVITIES OF SUBSTRATE BINDING BY THE MOLECULAR CHAPERONE HSP110/SSE1" (2017). The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access). 771. https://digitalcommons.library.tmc.edu/utgsbs_dissertations/771 This Dissertation (PhD) is brought to you for free and open access by the The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences at DigitalCommons@TMC. It has been accepted for inclusion in The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Dissertations and Theses (Open Access) by an authorized administrator of DigitalCommons@TMC. For more information, please contact [email protected]. ANALYSIS OF THE BIOCHEMICAL AND CELLULAR ACTIVITIES OF SUBSTRATE BINDING BY THE MOLECULAR CHAPERONE HSP110/SSE1 By Veronica Margarita Garcia, B.S. APPROVED: _______________________________ Supervisory Professor Kevin A. Morano, Ph.D. _______________________________ Catherine Denicourt, Ph.D. _______________________________ Theresa M. Koehler, Ph.D. _______________________________ Michael C. -
Sensitive Single Molecule Protein Quantification and Protein Complex
Proteomics 2011, 11, 4731–4735 DOI 10.1002/pmic.201100361 4731 TECHNICAL BRIEF Sensitive single-molecule protein quantification and protein complex detection in a microarray format Lee A. Tessler and Robi D. Mitra Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University, St. Louis, MO, USA Single-molecule protein analysis provides sensitive protein quantitation with a digital read- Received: June 30, 2011 out and is promising for studying biological systems and detecting biomarkers clinically. Accepted: October 3, 2011 However, current single-molecule platforms rely on the quantification of one protein at a time. Conventional antibody microarrays are scalable to detect many proteins simultaneously, but they rely on less sensitive and less quantitative quantification by the ensemble averaging of fluorescent molecules. Here, we demonstrate a single-molecule protein assay in a microarray format enabled by an ultra-low background surface and single-molecule imaging. The digital read-out provides a highly sensitive, low femtomolar limit of detection and four orders of magnitude of dynamic range through the use of hybrid digital-analog quantifica- tion. From crude cell lysate, we measured levels of p53 and MDM2 in parallel, proving the concept of a digital antibody microarray for use in proteomic profiling. We also applied the single-molecule microarray to detect the p53–MDM2 protein complex in cell lysate. Our study is promising for development and application of single-molecule protein methods because it represents a technological bridge between single-plex and highly multiplex studies. Keywords: Antibody microarray / Sandwich immunoassay / Single-molecule detection / Technology / Total internal reflection fluorescence Single-molecule protein detection has the potential to cence (TIRF) imaging has provided a platform for single- benefit systems biology and biomarker studies by providing molecule quantification on planar surfaces.