Heterologous protein expression in Pichia thermomethanolica BCC16875, a thermotolerant methylotrophic yeast and characterization of N-linked glycosylation in secreted protein Sutipa Tanapongpipat1, Peerada Promdonkoy1, Toru Watanabe2, Witoon Tirasophon3, Niran Roongsawang1, Yasunori Chiba2 & Lily Eurwilaichitr1 1Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand; 2Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan; and 3The Institute of Molecular Biosciences, Mahidol University, Nakhonpathom, Thailand Correspondence: Sutipa Tanapongpipat, Abstract Bioresources Technology Unit, National Center for Genetic Engineering and This study describes Pichia thermomethanolica BCC16875, a new methylotrophic Biotechnology, National Science and yeast host for heterologous expression. Both methanol-inducible alcohol Technology Development Agency, 113 oxidase (AOX1) and constitutive glyceraldehyde-3-phosphate dehydrogenase Phahonyothin Road, Khlong Nueng, Khlong (GAP) promoters from Pichia pastoris were shown to drive efficient gene Luang, Pathum Thani 12120, Thailand. expression in this host. Recombinant phytase and xylanase were expressed from Tel.: + 66 2 5646700 ext. 3472; both promoters as secreted proteins, with the former showing different patterns fax: + 66 2 5646707; e-mail:
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