1 Hop proanthocyanidins for the fining of beer 2 3 R. S.T. Linforth*a, K. Westwoodb, A. Somanic, N. Dohertyc, D.J. Cookc 4 5 6 aFood Sciences, School of Biosciences, Sutton Bonington Campus, University of Nottingham. 7 LE12 5RD. UK. 8 bBarth Innovations Ltd, Hop Pocket Lane, Paddock Wood, Kent, TN12 6DQ, United Kingdom 9 cBrewing Science Department, School of Biosciences, Sutton Bonington Campus, University of 10 Nottingham. 11 LE12 5RD. UK. 12 13 14 15 *Corresponding author:
[email protected] 16 17 18 1 19 Abstract 20 Fining agents are used in the clarification of beers; they help to reduce the time required to 21 sediment suspended yeast cells and ensure the clarity and colloidal stability of beer. 22 Following an adventitious observation during dry-hopping experiments, we identified a 23 fining activity associated with Saaz hops. Extracts of hop cones were subsequently shown to 24 have the capacity to flocculate yeast and result in their sedimentation. This activity has since 25 been identified in extracts of many different hop varieties and, significantly in spent hops, 26 the co-product resulting from commercial extraction of hops with either CO2 or ethanol. 27 Here we illustrate the activity of the novel finings extracted from spent hops following CO2 28 extraction of Galena hops. The sediments formed on fining were compact, relative to those 29 obtained when commercial isinglass was used to fine the same beers. The hop extracts were 30 also effective in reducing 90° haze in beers under conditions designed to mimic both cask 31 ale (12°C) and lager (4°C) type applications.