BD Stem Cell Resource Markers of Self-Renewal and Differentiation

23-9953-02 Self Renewing Pathway

TGF-β/Activin Sperm Liver Thymus Pancreas Thyroid Lung Intestine Wnt BMP-SMad Erk-MAPK Skin and Hair JAK-STAT Wnt TGF-β/Activin BMP4 bFGF LIF LIFR gp130 Egg

p p p Jak β-catenin Smads 175 MEK/ERK

Stat3 C-myc Sox2 Oct4 Nanog Smads 2/3 Neural Crest Primordial Germ Cell Stem Cell Neural Stem Cell Self Renewal Ectoderm Embryonic Stem Cell or Induced Pluripotent Endoderm Neurons, Glia Stem Cell Smooth Muscle Cardiac Tissue Chondrocytes Glial Restricted Progenitor Osteocytes

Neuronal Type 1 Astrocyte Oligodendrocyte Restricted Progenitor Progenitor Mesoderm Mesenchymal Stem Cell

Type 2 Astrocyte Endothelium Oligodendrocyte Neuron Heart Skeletal Muscle Kidney Smooth Muscle Myoblast Adipocyte (Fat) Chondrocyte Fibroblast Osteoblast (Bone) Hemangioblast (Cartilage)

Myotube (Muscle) Plasmacytoid Dendritic Cell Hematopoietic Stem Cell Committed Lymphoid Progenitor

Monoblast Megakaryoblast Proerythroblast Pre-NK Cell Thymocyte Pre B Cell Myeloblast

Erythroblast Progranulocyte Monocyte Myeloid Dendrtitic Cell Megakaryocyte NK Lymphoblast T-Lymphoblast B-Lymphoblast

Normoblast Neutrophilic Eosinophilic Basophilic Myelocyte Myelocyte Myelocyte NK Cell T Cell B Cell Macrophage Thrombocytes Reticulocyte Neutrophilic Eosinophilic Basophilic (Platelets) Band Cell Band Cell Band Cell Plasma Cell

Erythrocyte Neutrophil Eosinophil Basophil (Red Blood Cell)

White colored markers are available from BD Biosciences

Ectoderm Markers Embryonic Stem Cells Mesoderm Markers Endoderm Markers Stem Cell Relevant Signaling Astrocytes Neuronal Markers Embryonic Stem Cell Markers Early Mesoderm Markers Heart - Cardiogenesis Markers Hematopoietic Stem Cell Markers Mesenchymal Stem Cell Differentiation Markers Mesenchymal Stem Cell Markers (Fat) Definitive Endoderm Markers Erk-MAPK Signaling TGF Beta Signaling A2B5 GFAP α-Synuclein MAP2B Alkaline Phosphatase Nanog (Human) BMP2 Cryptic α-Actinin CD309 (VEGFR2, KDR) Negative Markers Chondrocyte (Cartilage) Markers Osteoblast (Bone) Markers Negative Markers B-Catenin HNF-1β (TCF-2) basic FGF Mek1 BMPR-II Id3 CD44 Ran-2 α-Synuclein (pY125) MASH1 CD9 Nanog (Mouse) BMP4 MIXL1 Annexin VI CD325 (N-Cadherin) CD184 (CXCR4) HNF-3β (FoxA2) C-Raf Mek1 (pS298) CD119 (IFN-γ receptor α-chain) p16 INK4 FGFR3 S100B b-Tubulin (Tuj1) mGluR1 CD15 (SSEA-1, Lewis X) Nucleostemin Brachyury NF-YA Cardiac specific myosin Connexin-43 CD2 CD24 Aggrecan Matrillin-4 Alkaline Phosphatase IGFBP-3 CD11b (Integrin αM chain) CD79a CD325 (N-Cadherin) Sox17 ERK Mek1/2 (pS222) c-Myc p107 CD24 mGluR1α CD24 Oct3/4 CD31 (PECAM1) Nodal Cardiac Troponin I Desmin CD3 CD33 CD44 Osteonectin Collagen 1 Osteocalcin CD14 CD117 (SCF R, c-kit) GATA4 ERK1 Mek2 DP-1 p300 Early Ectoderm Markers CD56 (NCAM) Nestin CD29 (Integrin β1) Oct3/4A CD34 (Mucosialin, gp 105-120) Sca-1 (Ly6A/E) Cardiac Troponin T Flk-1 (KDR, VEGF-R2, Ly-73) CD4 CD36 CD151 Sox9 Fibronectin RUNX2 CD19 HLA-DR ERK1/2 (pT202/pY204) p38 MAPK (PT180/pY182) E2F-1 PP2A Catalytic α Nestin TP63 CD81 (TAPA-1) NeuroD1 CD30 Podocalyxin CD325 (N-Cadherin) Snail Caveolin-2 GATA4 CD7 CD38 Collagen II CD45 (Leukocyte Common Hepatic Endoderm Markers ERK2 Ras E2F-2 ROCK-I Notch1 CD90 (Thy-1) Neurofilament NF-M CD49f (Integrin α6) Podocalyxin Associated Caveolin-3 GATA6 CD8 CD45 (Leukocyte Common Antigen, Ly-5) ERK3 E2F-3 ROCK-II Myoblast (Muscle) Markers α-Fetoprotein CD90 (Thy-1) CD90.1 (Thy-1.1) Neuron-restricted progenitors CD133 (AC133, Prominin-1) Keratin Sulfate CD13 HAND1 CD10 Antigen, Ly-5) Id1 Smad2/3 Endothelial Cell Markers Positive Markers Albumin CD326 (EpCAM) Neural Crest Cell Markers CD90.2 (Thy-1.2) Neuropilin-2 CD194 (CCR4) PRC2 (Suz12) CD31 (PECAM1) HAND2 CD11b CD45R (B220) Acetylcholinesterase Lactate Dehydrogenase (LDH) Jak-STAT Signaling Id2 SP1 CD14 CD151 ASGPR 1 GATA4 CD112 Nicastrin CD324 (E-Cadherin) Rex-1 CD34 (Mucosialin, gp 105-120) Islet 1 CD13 CD48 Actin Myf-5 CD9 CD73 (Ecto-5’-nucleotidase) CD49d (Integrin α4) MASH1 CD15 (SSEA-1, Lewis X) CD152 (CTLA-4) CD26 HNF-1α CD130 (gp130) STAT4 CD271 (p75, NGFR/NTR) p150 Glued CD326 (EpCAM) Ronin CD66 M-Cadherin CD14 CD56 (NCAM) B-Enolase (ENO-3) MyoD CD10 CD90 (Thy-1) Wnt Signaling CD57 (HNK-1) Neurogenin 3 CD18 (Integrin β2 chain, CR3/CR4) CD157 CD29 (Integrin β1) HNF-1β (TCF-2) STAT1 (C-TERMINUS) STAT4 (pY693) CD325 (N-Cadherin) Pax-5 CD338 (ABCG2) SFRP2 CD66c MLC2a CD15 (SSEA-1, Lewis X) CD97 CD146 (MCAM, MUC18) Pax3 CD13 CD105 (Endoglin) CD271 (p75, NGFR/NTR) Notch1 CD29 (Integrin β1) CD166 (ALCAM) CD44H (Pgp-1, H-CAM) LDLR STAT1 (pY701) STAT5 (MGF) 14-3-3 GSK-3β CD15s (Sialyl Lewis x) CD114 (G-CSF Receptor) CD325 (N-Cadherin) Pax7 CD29 (Integrin β1) CD106 (VCAM-1) ChAT P-glycoprotein c-Myc Smad2/3 CD31 (PECAM1) CD184 (CXCR4) CD106 (VCAM-1) MLC2v STAT3 STAT5 (MGF) (PY694) α-Catenin GSK-3β (pY216) CD16 CD138 (Syndecan-1) Desmin PK-M CD44 CD146 (MCAM, MUC18) CD49f (Integrin α6) Tat-SF1 Neural Stem Cell Markers Contactin PSD-95 Cripto Sox2 CD34 (Mucosialin, gp 105-120) CD192 (CCR2) CD117 (SCF R, c-kit) Myogenin STAT3 (pS727) STAT6 β-Catenin HDAC3 Doublecortin Serotonin Receptor 5-HT 2AR DPPA4 SSEA-3 CD140a (PDGFR ) Myosin Heavy Chain CD19 CD235a CD49d (Integrin α4) CD166 (ALCAM) A2B5 CD349 (Frizzled-9) CD36 CD201 (EPCR) a STAT3 (pY705) STAT6 (pY641) δ-Catenin Jagged1 GABA B Receptor Serotonin Receptor 5-HT 2BR DPPA5 (ESG1) SSEA-4 CD144 (VE-cadherin) NF-YA CD20 GATA3 CD49e (Integrin α5) Fibronectin Intestinal Stem Cell Markers BMI-1 Ki-67 CD45 (Leukocyte Common CD202b (TIE2) Mesenchymal Stem Cell Markers (Bone Marrow) γ-Catenin Lgr5 Gad65 SMN FGF-4 SSEA-5 CD166 (ALCAM) Nkx2.5 CD54 (ICAM-1) HLA I BMI-1 Lgr5 CD15 (SSEA-1, Lewis X) MSI1 (Musashi-1) Antigen, Ly-5) CD202b (TIE2) (pY992) Negative Markers Positive Markers Notch Signaling Akt Neurogenin 3 GAP-43 (Neuromodulin) Synapsin I FoxD3 STAT3 CD172a (SIRPA) P-glycoprotein (MDR) Positive Markers CD55 Vimentin EphB2 Sox9 CD15s (Sialyl Lewis x) MSl2 (Musashi-2) CD45R (B220) CD202b (TIE2) (pY1102) CBP (CREB-Binding Protein) Notch1 CaM Kinase II Nicastrin GluR δ 2 Synaptophysin Frizzled-5 STAT3 (pS727) CD202b (TIE2) SSEA-1 CD11a (Integrin αL chain) CD34 (Mucosialin, gp 105-120) CD10 CD117 (SCF R, c-kit) CD59 CD24 Nanog (Mouse) CD49d (Integrin α4) CD209 BMI-1 CDw93 (C1qRp) Jagged1 Notch2 Casein Kinase I ε Notch1 GluR2 Synaptotagmin GCNF (NR6A1) STAT3 (pY705) CD202b (TIE2) (pY992) Vimentin CD11b (Integrin αM chain) CD45 (Leukocyte Common CD13 CD120a (TNF Receptor Type I) Pancreatic Endoderm Markers CD29 (Integrin β1) Nestin CD49e (Integrin α5) CD209a (CIRE, DC-SIGN) CaM Kinase IV CDw338 (ABCG2) Jagged2 Notch3 Casein Kinase II α PP2A Catalytic α GluR5/6/7 Syntaxin GCTM-2 STAT3-interacting protein 1 CD202b (TIE2) (pY1102) CD14 Antigen, Ly-5) CD14 CD120b (TNF Receptor Type II) Skeletal Muscle Markers CD49f (Integrin 6) Neurogenin 3 CD49f (Integrin α6) Noggin CD49f (Integrin α6) CD213a1 CD31 (PECAM1) DAZL α Neurogenin 3 Notch4 Casein Kinase II β SNAI2 (Slug) Glutamine Synthetase Tau GDF-3 TRA-1-60 Antigen CD19 CD79a CD15 (SSEA-1, Lewis X) CD146 α-Actinin Myf5 CD142 Neuropilin-2 CD54 (ICAM-1) Notch1 CD54 (ICAM-1) CD213a2 CD34 (Mucosialin, gp 105-120) Flk-1 (KDR, VEGF-R2, Ly-73) Nicastrin CBP (CREB-Binding Protein) Wnt16 HNF-3 (FoxA2) TrkB Hsp27 TRA-1-81 Antigen Hemangioblast Markers CD81 (TAPA-1) Pax-6 b CD56 (NCAM) CD249 CD40f (Integrin a6 chain) FOXO1 CD31 (PECAM1) HLA-DR CD29 (Integrin β1) CD166 Actin MyoD Chromogranin A Nkx2.2 Islet-1 Tubby Lefty-A UTF-1 CD95 (Fas/APO-1) Prominin-2 CD62E (E-Selectin) CD252 (OX-40 Ligand) ACE CD324 (E-Cadherin) CD44 G-CSF CD44 (Pgp-1, H-CAM) CD166 (ALCAM) B-Enolase (ENO-3) Myogenin Gad65 Nkx6.1 Jagged1 Tyrosine Hydroxylase Lin-28 Zic3 CD133 (AC133, Prominin-1) PSA-NCAM CD62L (L-Selectin) CD253 (TRAIL) Brachury EphB4 CD59 Gfi-1 CD49d (Integrin α4) CD271 (p75, NGFR/NTR) CD146 (MCAM, MUC18) Myosin heavy chain Glucagon Pancreatic polypeptide MAP2 (a+b) Vimentin CD146 (MCAM, MUC18) Sox1 CD62P (P-Selectin) CD262 (TRAIL-R2, DR5) CD31 (PECAM1) Flk-1 (KDR, VEGF-R2, Ly-73) CD90 (Thy-1) HOXB4 CD49e (Integrin α5) Flk-1 (KDR, VEGF-R2, Ly-73) CD325 (N-Cadherin) Myosin light chain Glut2 Pax-6 Induced Pluripotency Markers CD51 (Integrin αV) GD2 Desmin Titin CD184 (CXCR4) Sox2 Oligodendrocyte Markers CD102 CD322 CD34 (Mucosialin, gp 105-120) GATA1 CD90.1 (Thy-1.1) Jak2 Insulin PDX-1 CD271 (p75, NGFR/NTR) Vimentin c-Myc Nanog CD105 (Endoglin) CD325 (N-Cadherin) CD133 (AC133, Prominin-1) LMO-2 CD105 (Endoglin) Lmo2 CD54 (ICAM-1) Ly-6A/E (Sca-1) M-Cadherin Islet-1 PTF1A CD338 (ABCG2) CD44 O1 KLF4 Oct3/4 CD106 (VCAM-1) CDw93 (C1qRp) CD144 (VE-cadherin) Podocalyxin CD106 (VCAM-1) Ly-6A/E (Sca-1) CD56 (NCAM) Nucleostemin Islet-2 Somatostatin CD44H (Pgp-1, H-CAM) O4 Lin-28 Sox2 CD109 CDw145 CD202b (TIE2) Runx1 (CBFA2) CD117 (SCF R, c-kit) MRP1 CD71 (Transferrin Receptor) SSEA-4 Smooth Muscle Markers NeuroD1 Synaptophysin CD73 (Ecto-5’-nucleotidase) STRO-1 CD140a (PDGFR α) Olig1 CD112 Flk-1 (KDR, VEGF-R2, Ly-73) CD202b (TIE2) (pY992) Tal (SCL) CD133 (AC133, Prominin-1) NF-YA Calmodulin Smooth muscle myosin CD90 (Thy-1) TAZ Primitive Gut Tube Markers Galactocerebroside C Olig2 Primordial Germ Cell Markers CD116 (GM-CSF Receptor) HIF-1α CD202b (TIE2) (pY1102) CD164 Notch1 Desmin Vimentin MBP RIP CD117 (SCF R, c-kit) IP-10 CD184 (CXCR4) P-glycoprotein CD105 (Endoglin) Vimentin CDX2 HNF-1β (TCF-2) Blimp-1 Nanos CD120a (TNF Receptor Type I) Ly-6A/E (Sca-1) CD201 (EPCR) Runx1 (CBFA2) CD106 (VCAM-1) CD15 (SSEA-1, Lewis X) Oct3/4 Skin Precursor Cell Markers CD120b (TNF Receptor Type II) Nestin CD202b (TIE2) Sox7 CD117 (SCF R, c-kit) Piwi/Hiwi CD121a (IL-1 Receptor, Type I/p80) STAT3 CD202b (TIE2) (pY992) Tal (SCL) CRABP2 Sca-1 (Ly6A/E) DAZL Steel CD124 (IL-4 Receptor ) STAT3 (pS727) CD202b (TIE2) (pY1102) WASP (Wiskott-Aldrich Fibronectin Vimentin FABP9 Stella (Dppa3) α CD141 (Thrombomodulin) STAT3 (pY705) CD325 (N-Cadherin) Syndrome Protein) Nestin Fragilis VASA (DDX4) CD144 (VE-cadherin) STAT3-interacting protein 1 Visual System Trophoblast Markers CD146 (MCAM, MUC18) Vimentin CD147 (Neurothelin) CD68 Pax-6 CDX2 Sox7 CD325 (N-Cadherin) Recoverin Cytokeratin 7 Trop-2