Suffolk County Community College s8

Suffolk County Community College Grant Campus BIO244 General Microbiology Spring Semester 2012 Laboratory Schedule Section: 21343, 24726 Tuesday, Thursday (Day) Instructor: P. Seshadri

Date Exercise # Topic(s) 1/24 1-2 Introduction; safety regulations, Physical and Chemical Control of Microorganisms, Culture Media and Tools used in the study of Clinically Significant Microorganisms, Movie - Microscope 1/26 3-1 Microscopy-The use of oil immersion objective lens in the study of microorganisms of medical importance: Bacillus sp., Escherichia coli, Borrelia burgdorferi and yeast. 1/31 12-3 Protozoa of Medical Importance: Entamoeba histolytica, Giardia lamblia, Trichomonas vaginalis, Balantidium coli, Plasmodium sp., Trypanosoma cruzi, and Trypanosoma gambiense. 2/2 12-4 Platyhelminths and Nematodes of Medical Importance: Clonorchis sinensis, Taenia saginata, Taenia solium, Enterobius vermicularis, Necator americanus, Trichinella spiralis, Ascaris lumbricoides, and Trichuris trichiura. 2/7 2-1, 3-3, 3-4 Wet Mounts, Ubiquity of Microorganisms 2/9 1-3, 1-4 Aseptic technique and Isolation of Bacteria including Clinically Significant Bacteria 2/14 2-2 Cultural Characteristics of Bacteria of Medical Importance: Bacillus subtilis, Escherichia coli, Proteus vulgaris, Streptococcus pyogenes, and Pseudomonas aeruginosa. 2/16 3-5, 3-6 Bacterial Smears, Simple Stain, Negative Stain 2/21 3-7 Gram Stain of Medically Significant Bacteria: Bacillus sp., Staphylococcus sp., Escherichia sp., and Neisseria sp. 2/23 3-8, 3-9 Staining Procedures of Clinical Significance 3-10 Capsule Stain of Klebsiella pneumonia, Endospore Stain of Bacillus subtilis. Prepared slides of: Acid-fast Stain of Mycobacterium tuberculosis, Flagella Stain of Proteus vulgaris 2/28 2-8 Culturing Anaerobes of Medical Importance- Clostridium sp.. Unknown Effects of Temperature on Pigmentation Gram stain of Morphological Unknown (End Material for Practical #1) 3/1 2-13, 7-3 Effects of UV Light and Antibiotics on the growth of Bacteria of Medical Importance(check results 28, 29), (Review for Practical) 3/8 ------Practical Exam #1 3/13 5-3, 5-7, Biochemical Tests used in the Identification of Bacteria of Medical Importance: 5-12 Exoenzymes – Carbohydrate Fermentation, ,Nitrate Reduction, Starch Hydrolysis (check results from previous lab session) 3/15 5-4, 5-8, Biochemical Tests used in the Identification of Bacteria of Medical Importance: 5-13, 5-20, Urea Hydrolysis, Hydrogen Sulfide Production, IMViC Test, Motility Test, (check 5-28 results from previous lab session) Date Exercise # Topic(s)

3/20 4-5, 4-6, Biochemical Tests used in the Identification of Bacteria of Medical Importance: 5-21, 5-25 TSI, Selective and Differential Media used in the identification of Bacteria of Medical Importance (check results from previous lab session) 3/22 5-29 API strips – Identification System for Enterobacteriaceae of Medical Importance Handout (check results from previous lab session)

3/27 4-4, 5-5, Pathogenic Staphylococci, Pyogenic Streptococci of Medical Importance) 5-24, (check results – API) 5-25, 5-27 3/29 Discuss Flow chart and Techniques used in the Identification of Unknown Bacteria 11-4 of Medical Importance. Agglutination Assays used in the Identification of Clinically Relevant Bacteria (Salmonella sp.) (Check results from previous lab session) 4/10 Appendix G Agarose Gel Electrophoresis of Lambda Phage DNA – Includes a Discussion on the Handout Clinical Significance of the Procedure 4/12 ______Identification of Clinically Significant Bacterial Unknowns (Gram Stain, MacConkey, BHIA) 4/17 ______Identification of Clinically Significant Bacterial Unknowns (Biochemical Tests)

4/19 ______Identification of Clinically Significant Bacterial Unknowns (results)

4/24 6-1 Assay of Bacteria in Milk – Plate Count, (Read gels) 4/26 Handout Rate of Respiration of Sugars by Saccharomyces cerevisisae – Computer Lab Introduces Students to the role of Computers in data collection and analysis in the research labs and Clinical Labs, (check results from previous lab session) 5/1 9-2 12-1, 12-2 Yeast of Medical Importance: Candida albicans Molds of Medical Importance: Aspergillus sp., Penicillium sp., and Rhizopus sp. Gram stain of yogurt, Gram stain colonies from 6-1. 5/3 Handout Genetic Transformation of Bacteria and Regulation of Gene Expression 10-3 Antibiotic Resistance and inducible genes (check results from previous lab session)

5/8 ------Results of Transformation and Gene Regulation Review for Practical

5/10 ------Practical Exam # 2

5/15 ------Clean-up, Check-out

Tuesday, Thursday (Day) P. Seshadri