Vibrio: Myths Vs. Facts “Flesh-Eating Bacteria” Is a Phrase Often At- Weakened Immune Systems Are at Higher Risk Inside This Issue
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ABSTRACT DRAKE, STEPHENIE LYNN. the Ecology Vibrio
ABSTRACT DRAKE, STEPHENIE LYNN. The Ecology Vibrio vulnificus and Vibrio parahaemolyticus from Oyster Harvest Sites in the Gulf of Mexico. (Under the direction of Dr. Lee-Ann Jaykus). The Vibrionaceae are environmentally ubiquitous to estuarine waters. Two species in particular, V. vulnificus and V. parahaemolyticus, are important human pathogens that are transmitted by the consumption of contaminated molluscan shellfish. There is limited information available for the recent risk assessments; accordingly, the purpose of this study was to address some of these data gaps in the V. vulnificus and V. parahaemolyticus risk assessments. The objectives of this study were to (i) quantify the levels of total estuarine bacteria, total Vibrio spp., and specific levels of non-pathogenic and pathogenic V. vulnificus and V. parahaemolyticus over the harvest period; and (ii) determine if length of harvest time affects the levels of V. vulnificus. Oyster and water samples were harvested seasonally from 3 U.S. Gulf Coast sites over 2 years. Environmental parameters were monitored during harvesting. Both surface and bottom water samples (1 L) were taken at the beginning of harvesting and at the end of harvesting. Oyster samples (15 specimens for each time point) were taken at 0, 2.5, 5.0, 7.5, and 10 hrs intervals after being held at ambient temperature during harvesting. Samples were processed for many different bacteria. For enumeration of total V. parahaemolyticus, pathogenic V. parahaemolyticus, and V. vulnificus was done using colony lift hybridization (tlh, tdh+ and/or trh+, and vvhA gene targets, respectively). MPN methods were also used to obtain estimates of pathogenic V. -
Kellie ID Emergencies.Pptx
4/24/11 ID Alert! recognizing rapidly fatal infections Susan M. Kellie, MD, MPH Professor of Medicine Division of Infectious Diseases, UNMSOM Hospital Epidemiologist UNMHSC and NMVAHCS Fever and…. Rash and altered mental status Rash Muscle pain Lymphadenopathy Hypotension Shortness of breath Recent travel Abdominal pain and diarrhea Case 1. The cross-country trucker A 30 year-old trucker driving from Oklahoma to California is hospitalized in Deming with fever and headache He is treated with broad-spectrum antibiotics, but deteriorates with obtundation, low platelet count, and a centrifugal petechial rash and is transferred to UNMH 1 4/24/11 What is your diagnosis? What is the differential diagnosis of fever and headache with petechial rash? (in the US) Tickborne rickettsioses ◦ RMSF Bacteria ◦ Neisseria meningitidis Key diagnosis in this case: “doxycycline deficiency” Key vector-borne rickettsioses treated with doxycycline: RMSF-case-fatality 5-10% ◦ Fever, nausea, vomiting, myalgia, anorexia and headache ◦ Maculopapular rash progresses to petechial after 2-4 days of fever ◦ Occasionally without rash Human granulocytotropic anaplasmosis (HGA): case-fatality<1% Human monocytotropic ehrlichiosis (HME): case fatality 2-3% 2 4/24/11 Lab clues in rickettsioses The total white blood cell (WBC) count is typicallynormal in patients with RMSF, but increased numbers of immature bands are generally observed. Thrombocytopenia, mild elevations in hepatic transaminases, and hyponatremia might be observed with RMSF whereas leukopenia -
Burkholderia Cenocepacia Integrates Cis-2-Dodecenoic Acid and Cyclic Dimeric Guanosine Monophosphate Signals to Control Virulence
Burkholderia cenocepacia integrates cis-2-dodecenoic acid and cyclic dimeric guanosine monophosphate signals to control virulence Chunxi Yanga,b,c,d,1, Chaoyu Cuia,b,c,1, Qiumian Yea,b, Jinhong Kane, Shuna Fua,b, Shihao Songa,b, Yutong Huanga,b, Fei Hec, Lian-Hui Zhanga,c, Yantao Jiaf, Yong-Gui Gaod, Caroline S. Harwoodb,g,2, and Yinyue Denga,b,c,2 aState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China; bGuangdong Innovative Research Team of Sociomicrobiology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China; cIntegrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; dSchool of Biological Sciences, Nanyang Technological University, Singapore 637551; eCenter for Crop Germplasm Resources, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; fState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; and gDepartment of Microbiology, University of Washington, Seattle, WA 98195 Contributed by Caroline S. Harwood, October 30, 2017 (sent for review June 1, 2017; reviewed by Maxwell J. Dow and Tim Tolker-Nielsen) Quorum sensing (QS) signals are used by bacteria to regulate N-octanoyl homoserine lactone (C8-HSL). The two QS systems biological functions in response to cell population densities. Cyclic have both distinct and overlapping effects on gene expression diguanosine monophosphate (c-di-GMP) regulates cell functions in (16, 17). response to diverse environmental chemical and physical signals One of the ways in which QS systems can act is by controlling that bacteria perceive. In Burkholderia cenocepacia, the QS signal levels of intracellular cyclic diguanosine monophosphate (c-di-GMP) receptor RpfR degrades intracellular c-di-GMP when it senses the in bacteria (18–21). -
Cholera Transmission: the Host, Pathogen and Bacteriophage Dynamic
REVIEWS Cholera transmission: the host, pathogen and bacteriophage dynamic Eric J. Nelson*, Jason B. Harris‡§, J. Glenn Morris Jr||, Stephen B. Calderwood‡§ and Andrew Camilli* Abstract | Zimbabwe offers the most recent example of the tragedy that befalls a country and its people when cholera strikes. The 2008–2009 outbreak rapidly spread across every province and brought rates of mortality similar to those witnessed as a consequence of cholera infections a hundred years ago. In this Review we highlight the advances that will help to unravel how interactions between the host, the bacterial pathogen and the lytic bacteriophage might propel and quench cholera outbreaks in endemic settings and in emergent epidemic regions such as Zimbabwe. 15 O antigen Diarrhoeal diseases, including cholera, are the leading progenitor O1 El Tor strain . Although the O139 sero- The outermost, repeating cause of morbidity and the second most common cause group caused devastating outbreaks in the 1990s, the El oligosaccharide portion of LPS, of death among children under 5 years of age globally1,2. Tor strain remains the dominant strain globally11,16,17. which makes up the outer It is difficult to gauge the exact morbidity and mortality An extensive body of literature describes the patho- leaflet of the outer membrane of Gram-negative bacteria. of cholera because the surveillance systems in many physiology of cholera. In brief, pathogenic strains har- developing countries are rudimentary, and many coun- bour key virulence factors that include cholera toxin18 Cholera toxin tries are hesitant to report cholera cases to the WHO and toxin co-regulated pilus (TCP)19,20, a self-binding A protein toxin produced by because of the potential negative economic impact pilus that tethers bacterial cells together21, possibly V. -
Vol. XXV Issue No. 2 June 2004
Vol. XXV Issue No. 2 June 2004 Report on the Enhanced Surveillance for Chlamydia West Nile Virus - 2003 Summary and in Women - South Carolina, 1998-2002 the Upcoming Season WAYNE A. DUFFUS, MD, PhD LENA M. BRETOUS, MD, MPH Medical Consultant Medical Consultant Extent of Chlamydia Problem Chlamydia remains a significant health threat among In 2003, South Carolina recorded six human cases of women and adolescents. Most estimates predict that 1 in 20 West Nile Virus (WNV). Surveillance numbers for other WNV sexually active woman of childbearing age or 1 in 10 adoles- positives in 2003 include: 282 birds, 54 equine, 3 mosquito cent girls are infected with chlamydia. In fact, 90% of all pools, and 1 alpaca. See table on page reported cases are in individuals less than 24 years of age. three for human case description. INSIDE THIS ISSUE Unfortunately, 60 – 80% of infected women have no symp- Because of the long stretch of toms, and therefore are not aware of their infection and may warm weather in spring and fall, in- Report on the Enhanced not seek health care. fected mosquitoes have a longer win- Surveillance for Chlamydia is the most commonly reported sexually dow to spread the disease in South Chlamydia in Women - South Carolina, transmitted infection among women in the United States (US). Carolina. The first human WNV case 1998 - 2002 The southeast led the nation in chlamydia prevalence in 2002. in South Carolina during 2003 was also Pg. 1 For example, there were 451.1 cases/100,000 persons diag- the first recorded case in the country nosed in the U.S. -
WO 2014/134709 Al 12 September 2014 (12.09.2014) P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2014/134709 Al 12 September 2014 (12.09.2014) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A61K 31/05 (2006.01) A61P 31/02 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, PCT/CA20 14/000 174 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 4 March 2014 (04.03.2014) KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (30) Priority Data: ZW. 13/790,91 1 8 March 2013 (08.03.2013) US (84) Designated States (unless otherwise indicated, for every (71) Applicant: LABORATOIRE M2 [CA/CA]; 4005-A, rue kind of regional protection available): ARIPO (BW, GH, de la Garlock, Sherbrooke, Quebec J1L 1W9 (CA). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (72) Inventors: LEMIRE, Gaetan; 6505, rue de la fougere, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Sherbrooke, Quebec JIN 3W3 (CA). -
Inverse Regulation of Vibrio Cholerae Biofilm Dispersal by Polyamine Signals Andrew a Bridges1,2, Bonnie L Bassler1,2*
RESEARCH ARTICLE Inverse regulation of Vibrio cholerae biofilm dispersal by polyamine signals Andrew A Bridges1,2, Bonnie L Bassler1,2* 1Department of Molecular Biology, Princeton University, Princeton, United States; 2The Howard Hughes Medical Institute, Chevy Chase, United States Abstract The global pathogen Vibrio cholerae undergoes cycles of biofilm formation and dispersal in the environment and the human host. Little is understood about biofilm dispersal. Here, we show that MbaA, a periplasmic polyamine sensor, and PotD1, a polyamine importer, regulate V. cholerae biofilm dispersal. Spermidine, a commonly produced polyamine, drives V. cholerae dispersal, whereas norspermidine, an uncommon polyamine produced by vibrios, inhibits dispersal. Spermidine and norspermidine differ by one methylene group. Both polyamines control dispersal via MbaA detection in the periplasm and subsequent signal relay. Our results suggest that dispersal fails in the absence of PotD1 because endogenously produced norspermidine is not reimported, periplasmic norspermidine accumulates, and it stimulates MbaA signaling. These results suggest that V. cholerae uses MbaA to monitor environmental polyamines, blends of which potentially provide information about numbers of ‘self’ and ‘other’. This information is used to dictate whether or not to disperse from biofilms. Introduction Bacteria frequently colonize environmental habitats and infection sites by forming surface-attached multicellular communities called biofilms. Participating in the biofilm lifestyle allows bacteria to col- lectively acquire nutrients and resist threats (Flemming et al., 2016). By contrast, the individual free- *For correspondence: swimming state allows bacteria to roam. The global pathogen Vibrio cholerae undergoes repeated [email protected] rounds of clonal biofilm formation and disassembly, and both biofilm formation and biofilm exit are central to disease transmission as V. -
Vibrio Vulnificus Fact Sheet
DISTRICT OF COLUMBIA DEPARTMENT OF HEALTH Division of Epidemiology, Disease Surveillance and Investigation 899 N. Capitol Street, NE, Suite 580 Washington, D.C. 20002 (202) 442-9371 * Fax (202) 442-8060 www.dchealth.dc.gov What is the treatment for Vibrio vulnificus What is Vibrio vulnificus? infection? Vibrio vulnificus is a bacterium in the same family that Vibrio vulnificus infection is treated with antibiotics, causes cholera. The bacterium lives in warm seawater such as tetracycline, doxycycline, or cephalosporin and is a salt-requiring organism. It is frequently isolated (e.g., ceftazidime). from oysters and other shellfish in warm coastal waters during the summer months. How can Vibrio vulnificus infection be prevented? What is Vibrio vulnificus infection? • Do not eat raw oysters or other raw Vibrio vulnificus infection is an acute illness that shellfish. produces septicemia (blood infection) in persons with • Cook shellfish (oysters, clams, mussels) chronic liver disease, chronic alcoholism, or those who thoroughly. Boil until the shells open and are immunocompromised. When open wounds are continue boiling for 5 minutes, or steam exposed to warm seawater, Vibrio vulnificus infections until the shells open during cooking. Do not may lead to skin breakdown and ulceration. Bloodstream eat shellfish that do not open during infections, common in immunocompromised persons, cooking. Boil shucked oysters for at least 3 can be fatal. The majority of infections in the United minutes, or fry them in oil at least 10 States are reported from the Gulf Coast states. minutes at 375°F. • Avoid cross-contamination of cooked How does Vibrio vulnificus infection occur? seafood and other foods with raw seafood Infection occurs after eating contaminated raw or and their juices. -
Investigation Into Rising Vibrio Populations in Eastern Coast Oysters Eric Grigg, Donna L
Investigation into Rising Vibrio Populations in Eastern Coast Oysters Eric Grigg, Donna L. Rhoads, M.Sc. Introduction Results and Discussion Conclusion Vibrio parahaemolyticus is a gram-negative, rod-shaped, and halophilic bacteria that is the leading cause of Oyster Collection: The study found an increasing trend of both Vibrio parahaemolyticus CFUs and Vibrio vulnificus CFUs. This seafood-borne bacterial gastroenteritis. The gastroenteritis is caused when Vibrio parahaemolyticus is consumed with shows that the risk of infection with Vibrio bacteria via raw oysters rises during the summer months. In addition, raw oysters and various other forms of seafood. The gastroenteritis is mainly characterized by watery diarrhea along Our results show an overall increase in Vibrio CFUs over the course of the summer. Our first four collections show a constant heavy rainfall seems to have an effect on Vibrio populations. After Hurricane Arthur passed, the CFUs for Vibrio with nausea, vomiting, abdominal cramps, chills, and fever.1 Vibrio vulnificus causes the same gastroenteritis, but can increase in number of Vv CFUs until our fifth collection. The fifth collection was performed after Hurricane Arthur passed through the vulnificus dropped sharply. This occurred again after heavy rainfall during the eighth week of the study. Given these infect the bloodstream of immunocompromised people causing fever, chills, lowered blood pressure, and skin lesions.2 Connecticut area around the 4th of July. The hurricane contributed to the drop in Vv numbers by decreasing the salinity of the water results, routine surveillance of oyster beds should be instituted. In addition, a more thorough means of cleaning the Fifty percent of people infected with Vibrio vulnificus succumb to the infection.2 and increasing the amount of sediment and runoff from the mainland. -
Kentucky Reportable Disease Form Department for Public Health Division of Epidemiology and Health Planning 275 East Main St., Mailstop HS1E-C
EPID 200 (Rev. Jan/03) Kentucky Reportable Disease Form Department for Public Health Division of Epidemiology and Health Planning 275 East Main St., Mailstop HS1E-C Frankfort, KY 40621-0001 Disease Name_____________________ Mail Form to Local Health Department DEMOGRAPHIC DATA Patient’s Last Name First M.I. Date of Birth Age Gender / / M F Unk Address City State Zip County of Residence Phone Number Patient ID Number Ethnic Origin Race His. Non-His. W B A/PI Am.Ind. Other DISEASE INFORMATION Disease/Organism Date of Onset Date of Diagnosis / / / / List Symptoms/Comments Highest Temperature Days of Diarrhea Hospitalized? Admission Date Discharge Date Died? Date of Death Yes No / / / / Yes No Unk / / Hospital Name: Is Patient Pregnant? Yes No If yes, # wks_____ School/Daycare Associated? Yes No Outbreak Associated? Yes No Name of School/Daycare: Food Handler? Yes No Person or Agency Completing form: Attending Physician: Name: Agency: Name: Address: Address: Phone: Date of Report: / / Phone: LABORATORY INFORMATION Date Name or Type of Test Name of Laboratory Specimen Source Results ADDITIONAL INFORMATION FOR SEXUALLY TRANSMITTED DISEASES ONLY Method of case detection: Prenatal Community & Screening Delivery Instit. Screening Reactor Provider Report Volunteer Disease: Stage Disease: Site: (Check all that apply) Resistance: Primary (lesion) Secondary (symptoms) Gonorrhea Genital, uncomplicated Ophthalmic Penicillin Syphilis Early Latent Late Latent Chlamydia Pharyngeal PID/Acute Tetracycline Congenital Other Chancroid Anorectal Salpingitis Other ___________ Other___________________ Date of spec. Laboratory Name Type of Test Results Treatment Date Medication Dose Collection If syphilis, was previous treatment given for this infection? Yes No If yes, give approximate date and place_______________________________________________________________ 902 KAR 2:020 requires health professionals to report the following diseases to the local health departments serving the jurisdiction in which the patient resides or to the Kentucky Department for Public Health (KDPH). -
Calhoun County Reportable Disease
Page 1 of 5 FLORIDA DEPARTMENT OF HEALTH Date: 10/13/2014 MERLIN Registry System Time: 04:14:54 pm CONFIRMED, PROBABLE, SUSPECT, UNKNOWN CASES OF MULTIPLE DISEASES WITH REPORT DATE FROM 12/29/2013 TO Period Comparison Selection Date Compare Date 1 Compare Date 2 Compare Date 3 12/29/2013 - 10/13/2014 12/29/2012 - 10/13/2013 12/29/2011 - 10/13/2012 12/29/2010 - 10/13/2011 Disease Name Cases Rates* Cases Rates* Cases Rates* Cases Rates* County: CALHOUN Brucellosis - 02300 0 0.00 0 0.00 1 6.81 0 0.00 Campylobacteriosis - 03840 0 0.00 0 0.00 3 20.44 0 0.00 Cryptosporidiosis - 13680 1 6.82 0 0.00 0 0.00 0 0.00 Giardiasis, Acute - 00710 0 0.00 0 0.00 1 6.81 0 0.00 Haemophilus influenzae Invasive Disease - 0 0.00 1 6.77 0 0.00 0 0.00 03841 Hepatitis B, Chronic - 07032 1 6.82 0 0.00 2 13.63 0 0.00 Hepatitis C, Chronic - 07054 22 149.95 11 74.44 11 74.95 15 102.46 Lead Poisoning - 94890 0 0.00 1 6.77 0 0.00 1 6.83 Meningococcal Disease - 03630 0 0.00 0 0.00 0 0.00 1 6.83 Rabies, Animal - 07102 0 0.00 0 0.00 0 0.00 1 6.83 Rabies, Possible Exposure - 07101 1 6.82 0 0.00 4 27.26 2 13.66 Salmonellosis - 00300 1 6.82 3 20.30 4 27.26 3 20.49 Varicella (Chickenpox) - 05290 0 0.00 0 0.00 0 0.00 2 13.66 Vibriosis (Vibrio parahaemolyticus) - 00540 0 0.00 1 6.77 0 0.00 0 0.00 Total: 26 177.23 17 115.05 26 177.16 25 170.77 STATEWIDE TOTAL Amebic Encephalitis - 13620 1 0.01 1 0.01 0 0.00 1 0.01 Anthrax - 02200 0 0.00 0 0.00 0 0.00 1 0.01 Arboviral Disease, Other - 06000 1 0.01 0 0.00 0 0.00 0 0.00 Arsenic Poisoning - 98080 2 0.01 12 0.06 6 0.03 4 0.02 Botulism, -
Vibrio Inhibens Sp. Nov., a Novel Bacterium with Inhibitory Activity Against Vibrio Species
The Journal of Antibiotics (2012) 65, 301–305 & 2012 Japan Antibiotics Research Association All rights reserved 0021-8820/12 www.nature.com/ja ORIGINAL ARTICLE Vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against Vibrio species Jose´ Luis Balca´zar1,2, Miquel Planas1 and Jose´ Pintado1 Strain BFLP-10T, isolated from faeces of wild long-snouted seahorses (Hippocampus guttulatus), is a Gram-negative, motile and facultatively anaerobic rod. This bacterium produces inhibitory activity against Vibrio species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-10T was a member of the genus Vibrio and was most closely related to Vibrio owensii (99%), Vibrio communis (98.9%), Vibrio sagamiensis (98.9%) and Vibrio rotiferianus (98.4%). However, multilocus sequence analysis using gyrB, pyrH, recA and topA genes revealed low levels of sequence similarity (o91.2%) with these closely related species. In addition, strain BFLP-10T could be readily differentiated from other closely related species by several phenotypic properties and fatty acid profiles. The G þ C content of the DNA was 45.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-10T represents a novel species within the genus Vibrio, for which the name Vibrio inhibens sp. nov. is proposed. The type strain is BFLP-10T (¼ CECT 7692T ¼ DSM 23440T). The Journal of Antibiotics (2012) 65, 301–305; doi:10.1038/ja.2012.22; published online 4 April 2012 Keywords: polyphasic taxonomic analysis; seahorses; Vibrio inhibens INTRODUCTION Physiological and biochemical characterization The family Vibrionaceae currently comprises six validly published Gram reaction was determined using the non-staining (KOH) method.13 Cell genera: Vibrio,1 Photobacterium,2 Salinivibrio,3 Enterovibrio,4 Grimontia5 morphology and motility were studied using phase-contrast microscopy and 14 and Aliivibrio.6 Vibrio species are common inhabitants of aquatic electron microscopy as previously described by Herrera et al.